##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139234_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9219827
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5127655865994	33.0	33.0	33.0	33.0	33.0
2	32.53227994408138	33.0	33.0	33.0	33.0	33.0
3	32.520658467886655	33.0	33.0	33.0	33.0	33.0
4	36.42401674131196	37.0	37.0	37.0	37.0	37.0
5	36.463873345996625	37.0	37.0	37.0	37.0	37.0
6	36.462786340784916	37.0	37.0	37.0	37.0	37.0
7	36.44098755866027	37.0	37.0	37.0	37.0	37.0
8	36.43666198942778	37.0	37.0	37.0	37.0	37.0
9	36.46031319242758	37.0	37.0	37.0	37.0	37.0
10-11	36.407616325121936	37.0	37.0	37.0	37.0	37.0
12-13	36.387230313540584	37.0	37.0	37.0	37.0	37.0
14-15	38.2928790312443	40.0	37.0	40.0	37.0	40.0
16-17	38.25025556336361	40.0	37.0	40.0	37.0	40.0
18-19	38.292789767096494	40.0	37.0	40.0	37.0	40.0
20-21	38.297484094139094	40.0	37.0	40.0	37.0	40.0
22-23	38.296183248518915	40.0	37.0	40.0	37.0	40.0
24-25	38.26681697556282	40.0	37.0	40.0	37.0	40.0
26-27	38.13378043192934	40.0	37.0	40.0	33.0	40.0
28-29	38.05800243729802	40.0	37.0	40.0	33.0	40.0
30-31	37.95968386548965	40.0	37.0	40.0	33.0	40.0
32-33	37.87045457097312	40.0	37.0	40.0	33.0	40.0
34-35	37.818585079562176	40.0	37.0	40.0	33.0	40.0
36-37	37.73974507442482	40.0	37.0	40.0	33.0	40.0
38-39	37.66848387616214	40.0	37.0	40.0	33.0	40.0
40-41	37.65580763746673	40.0	37.0	40.0	33.0	40.0
42-43	37.60002948512735	40.0	37.0	40.0	33.0	40.0
44-45	37.51723820053029	40.0	37.0	40.0	33.0	40.0
46-47	37.46610491172403	40.0	37.0	40.0	33.0	40.0
48-49	37.46263755183128	40.0	37.0	40.0	33.0	40.0
50-51	37.34396741128951	40.0	37.0	40.0	33.0	40.0
52-53	37.2739947289719	40.0	37.0	40.0	33.0	40.0
54-55	37.1076920459345	40.0	37.0	40.0	33.0	40.0
56-57	36.980284975210026	37.0	37.0	40.0	33.0	40.0
58-59	36.879169523505475	37.0	37.0	40.0	33.0	40.0
60-61	36.74194084536048	37.0	37.0	40.0	33.0	40.0
62-63	36.611834943731736	37.0	37.0	40.0	33.0	40.0
64-65	36.44682589250449	37.0	37.0	40.0	33.0	40.0
66-67	36.245342217050904	37.0	37.0	40.0	33.0	40.0
68-69	36.027675714859	37.0	37.0	40.0	33.0	40.0
70-71	35.771809294329806	37.0	35.0	37.0	33.0	40.0
72-73	35.5761082155465	37.0	33.0	37.0	33.0	40.0
74-75	35.38610337291672	37.0	33.0	37.0	33.0	40.0
76-77	33.5732639759498	35.0	33.0	37.0	27.0	37.0
78-79	34.876306600885655	37.0	33.0	37.0	33.0	37.0
80-81	34.94087176070812	37.0	33.0	37.0	33.0	37.0
82-83	34.8139510140074	37.0	33.0	37.0	33.0	37.0
84-85	34.67830442591823	37.0	33.0	37.0	33.0	37.0
86-87	34.585056432444844	37.0	33.0	37.0	33.0	37.0
88-89	34.46852403855358	37.0	33.0	37.0	30.0	37.0
90-91	34.41148470614675	37.0	33.0	37.0	30.0	37.0
92-93	34.35678209542992	37.0	33.0	37.0	30.0	37.0
94-95	34.3764892233755	37.0	33.0	37.0	33.0	37.0
96-97	34.36627926117502	37.0	33.0	37.0	33.0	37.0
98-99	34.36832040350066	37.0	33.0	37.0	33.0	37.0
100	34.350454118119515	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	0.0
14	1.0
15	0.0
16	0.0
17	1.0
18	4.0
19	19.0
20	101.0
21	574.0
22	2017.0
23	5605.0
24	11605.0
25	21798.0
26	37344.0
27	58593.0
28	86561.0
29	121542.0
30	163926.0
31	218156.0
32	285339.0
33	375273.0
34	502103.0
35	706206.0
36	1176715.0
37	2615207.0
38	2766628.0
39	64507.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.82101540516975	20.480525285344292	14.35387019734752	24.344589112138436
2	14.418448415572222	20.734336989186456	42.2579729532886	22.589241641952718
3	17.733174386026985	26.834711757606733	31.096212542816694	24.335901313549595
4	12.06842021004515	18.587167175792093	38.21204347371485	31.132369140447903
5	13.002196245113925	37.97917249423443	35.57763068656277	13.441000574088863
6	29.223871376119163	37.97233609535639	18.684122843285742	14.119669685238701
7	25.597909808936762	33.1236150092621	22.668917757350545	18.60955742445059
8	24.351465596914128	36.45980559071227	21.35599724376607	17.832731568607525
9	25.30723190359212	16.68947801298224	20.625159235634246	37.3781308477914
10-11	24.061999479470852	26.53251173102299	28.696281654970306	20.709207134535852
12-13	25.187658117155152	24.067598896942187	28.426602457204993	22.31814052869767
14-15	22.386084374151782	25.496806704551094	26.099347511838094	26.017761409459034
16-17	21.93683786040671	28.737643341897844	26.727941858345066	22.59757693935038
18-19	21.82413509494267	27.321505056439776	28.83989037972188	22.014469468895676
20-21	22.59718783652988	26.75165014716272	28.647229397639677	22.003932618667722
22-23	22.455272832198137	27.104571483738976	28.409483146701202	22.03067253736168
24-25	22.28908525383721	27.284201807732185	28.671513874802372	21.755199063628233
26-27	22.136549735562124	27.619987459456784	28.712080567726044	21.531382237255052
28-29	21.842915319047048	27.170802800156117	28.9811444489838	22.005137431813033
30-31	22.24545309500807	27.557079048373833	28.47294750725448	21.724520349363615
32-33	22.78160291918696	27.020216932438156	28.580112343615276	21.618067804759605
34-35	22.153999869224076	27.078774985246667	28.81999897677414	21.947226168755115
36-37	21.924497344968106	27.45610516595956	28.639115556725454	21.98028193234688
38-39	21.973293143213475	26.928717000706108	28.833265613541865	22.264724242538552
40-41	22.138131493314166	26.871731351500138	28.93088525980383	22.059251895381866
42-43	22.341271853574433	26.76394212948212	28.799699239887378	22.095086777056068
44-45	22.04514264703744	27.31928483304075	28.41551525528499	22.220057264636818
46-47	22.587914953743432	26.89183182884365	27.719889989814572	22.800363227598353
48-49	22.35039793986306	26.96294258701002	27.944498184312366	22.74216128881455
50-51	22.210414162402504	27.260574230908887	28.0025813694894	22.526430237199214
52-53	22.339982284660493	27.485382034434835	27.999440716145646	22.17519496475903
54-55	22.294272649140133	27.450905748409422	27.63552820460519	22.61929339784525
56-57	22.622440924889613	27.80853673102584	27.15391677690937	22.41510556717518
58-59	22.23986423839605	28.06684342654806	27.70281709782006	21.99047523723583
60-61	22.587212821748313	28.304206072564064	27.062212250442823	22.0463688552448
62-63	22.295004789786308	28.363633037105174	27.115458254815895	22.225903918292627
64-65	22.37914781401253	28.087969034975142	27.186386141117367	22.346497009894957
66-67	22.566278701941663	27.93371125583077	27.04111413493741	22.458895907290156
68-69	22.544107085303306	27.680691856938743	27.262207486023026	22.51299357173492
70-71	22.543765383547925	27.345394643124095	27.362251922533343	22.748588050794638
72-73	22.52452953620729	27.501972645975805	27.353707695980507	22.619790121836402
74-75	22.517307977857307	27.583325520291574	27.232334799448722	22.6670317024024
76-77	22.480190097231162	27.54357468623912	27.18875956639361	22.78747565013611
78-79	22.551974137581045	27.584483928463865	27.106953669534516	22.75658826442057
80-81	22.572540320873273	27.473822452553193	27.17097706463078	22.782660161942754
82-83	22.69869708703339	27.405674159877623	27.31072106348551	22.58490768960348
84-85	22.887560941576282	27.330800150052102	27.107540555875097	22.674098352496515
86-87	22.546575736810862	27.47328238315678	27.23301605706372	22.74712582296864
88-89	22.695115838292715	27.327424791086933	27.20279453496641	22.774664835653937
90-91	22.71276242007304	27.247435050280234	27.34360916830683	22.6961933613399
92-93	22.834844502856548	27.27436281515192	27.26903720230863	22.621755479682896
94-95	22.667646177870704	27.353364119355938	27.2633833412334	22.71560636153996
96-97	22.64313063961303	27.41218504824815	27.455261899248057	22.489422412890764
98-99	23.82070838965875	29.316984954914975	27.613319546889258	19.248987108537012
100	22.72048004239483	30.46439258313424	21.310206896015977	25.504920478454952
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1665.0
1	1673.0
2	2855.5
3	4317.5
4	6214.0
5	10578.0
6	13985.5
7	15841.0
8	16627.5
9	15843.0
10	14189.0
11	12613.0
12	13884.0
13	19050.0
14	30465.5
15	45821.0
16	58320.5
17	65658.5
18	67353.5
19	63410.0
20	56357.0
21	48672.5
22	43192.5
23	42434.5
24	46215.0
25	55655.5
26	70862.5
27	87840.0
28	101967.5
29	117582.0
30	133654.0
31	148388.5
32	166864.5
33	187083.0
34	208434.0
35	229779.5
36	254329.5
37	275713.0
38	285407.0
39	294928.0
40	311916.5
41	324845.0
42	331642.0
43	342039.5
44	356188.5
45	372145.5
46	395387.0
47	418700.5
48	420825.5
49	417770.0
50	421696.0
51	418350.5
52	398953.0
53	371440.0
54	345610.5
55	318017.5
56	291393.0
57	263614.0
58	232589.0
59	197211.0
60	157714.5
61	122199.0
62	92812.0
63	68580.0
64	50707.0
65	37347.0
66	27445.0
67	20935.0
68	15786.5
69	11365.5
70	8090.5
71	5578.0
72	3800.0
73	2613.5
74	1741.0
75	1147.0
76	768.0
77	501.5
78	356.5
79	262.5
80	185.5
81	122.5
82	82.5
83	54.5
84	36.0
85	29.5
86	25.5
87	19.0
88	12.0
89	8.0
90	4.5
91	3.0
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.001030388097303778
5	0.0
6	1.626928574690176E-4
7	0.0
8	0.0
9	0.0
10-11	0.003931744055501259
12-13	2.169238099586901E-5
14-15	0.0012961197645031735
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	140.0
20-21	692.0
22-23	1517.0
24-25	3024.0
26-27	5339.0
28-29	9448.0
30-31	22932.0
32-33	30103.0
34-35	38965.0
36-37	52933.0
38-39	59596.0
40-41	64217.0
42-43	72955.0
44-45	84171.0
46-47	80459.0
48-49	74393.0
50-51	63765.0
52-53	65112.0
54-55	78445.0
56-57	84600.0
58-59	79821.0
60-61	83204.0
62-63	84692.0
64-65	87194.0
66-67	93654.0
68-69	70948.0
70-71	69845.0
72-73	66659.0
74-75	68884.0
76-77	66978.0
78-79	73352.0
80-81	75951.0
82-83	73837.0
84-85	74923.0
86-87	79872.0
88-89	86624.0
90-91	91641.0
92-93	95663.0
94-95	112006.0
96-97	306549.0
98-99	563247.0
100-101	5921477.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.220949387157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.14143393765295	70.77709995374396
2	8.526601247227985	13.850772966911482
3	2.347853723341341	5.720847252958655
4	0.9337690404236241	3.0336643188616534
5	0.4175168504576341	1.6955557489652349
6	0.22464740926828544	1.0947645514881217
7	0.1270042633186817	0.7220784790071865
8	0.08079767255996327	0.524997093887429
9	0.05236441902990849	0.3827779044944471
>10	0.1443706554383406	1.7230855135927698
>50	0.002236361404242104	0.12134835345636542
>100	0.0012656628246076782	0.18145999140211708
>500	4.6252351586314685E-5	0.03493693710626957
>1k	9.250470089866168E-5	0.1366109341242114
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0846190497934505E-5	0.0
2	0.0	0.0	0.0	2.169238099586901E-5	1.0846190497934505E-5
3	0.0	0.0	0.0	2.169238099586901E-5	1.0846190497934505E-5
4	0.0	0.0	0.0	2.169238099586901E-5	1.0846190497934505E-5
5	0.0	0.0	0.0	2.169238099586901E-5	1.0846190497934505E-5
6	0.0	0.0	0.0	2.169238099586901E-5	2.169238099586901E-5
7	0.0	0.0	0.0	3.253857149380352E-5	2.169238099586901E-5
8	0.0	0.0	0.0	3.253857149380352E-5	4.338476199173802E-5
9	0.0	0.0	0.0	5.423095248967253E-5	4.338476199173802E-5
10-11	0.0	0.0	0.0	5.9654047738639785E-5	4.338476199173802E-5
12-13	0.0	0.0	0.0	7.592333348554153E-5	4.338476199173802E-5
14-15	1.0846190497934505E-5	0.0	0.0	8.13464287345088E-5	5.423095248967253E-5
16-17	2.169238099586901E-5	0.0	0.0	1.247311907262468E-4	7.592333348554153E-5
18-19	2.169238099586901E-5	0.0	0.0	1.626928574690176E-4	8.676952398347604E-5
20-21	2.169238099586901E-5	0.0	0.0	1.8980833371385386E-4	8.676952398347604E-5
22-23	2.169238099586901E-5	0.0	0.0	2.3319309570559186E-4	9.219261923244329E-5
24-25	2.7115476244836265E-5	0.0	0.0	3.036933339421662E-4	1.0303880973037781E-4
26-27	3.253857149380352E-5	0.0	0.0	6.941561918678083E-4	1.0846190497934506E-4
28-29	3.253857149380352E-5	0.0	0.0	0.0020499300041096217	1.0846190497934506E-4
30-31	3.253857149380352E-5	0.0	0.0	0.005726788582909419	1.0846190497934506E-4
32-33	5.423095248967253E-5	0.0	0.0	0.012944928359284832	1.1388500022831231E-4
34-35	6.507714298760703E-5	0.0	0.0	0.021670688614873142	1.1930809547727957E-4
36-37	7.592333348554153E-5	0.0	0.0	0.03173595339695637	1.1930809547727957E-4
38-39	7.592333348554153E-5	0.0	0.0	0.0472622750947496	1.1930809547727957E-4
40-41	7.592333348554153E-5	0.0	0.0	0.061161668217852676	1.1930809547727957E-4
42-43	7.592333348554153E-5	0.0	0.0	0.07489836848348673	1.2473119072624683E-4
44-45	8.13464287345088E-5	0.0	0.0	0.08827172136743996	1.3015428597521408E-4
46-47	9.219261923244329E-5	0.0	0.0	0.10079364829730536	1.3015428597521408E-4
48-49	9.761571448141055E-5	0.0	0.0	0.1098936021250724	1.4100047647314857E-4
50-51	9.761571448141055E-5	0.0	0.0	0.11607050761364611	1.4100047647314857E-4
52-53	9.761571448141055E-5	0.0	0.0	0.12188948881578798	1.5184666697108307E-4
54-55	9.761571448141055E-5	0.0	0.0	0.12737766120774283	1.626928574690176E-4
56-57	9.761571448141055E-5	0.0	0.0	0.13194390740737327	1.7353904796695209E-4
58-59	9.761571448141055E-5	0.0	0.0	0.1364938843212568	1.843852384648866E-4
60-61	1.0846190497934506E-4	0.0	0.0	0.1413584007595804	1.843852384648866E-4
62-63	1.0846190497934506E-4	0.0	0.0	0.14578364648273767	1.952314289628211E-4
64-65	1.0846190497934506E-4	0.0	0.0	0.15020346911064603	1.952314289628211E-4
66-67	1.0846190497934506E-4	0.0	0.0	0.15468836888154192	2.0607761946075563E-4
68-69	1.0846190497934506E-4	0.0	0.0	0.15940103865289446	2.2234690520765737E-4
70-71	1.0846190497934506E-4	0.0	0.0	0.16391847699528417	2.3861619095455914E-4
72-73	1.0846190497934506E-4	0.0	0.0	0.1671343724779218	2.8200095294629715E-4
74-75	1.0846190497934506E-4	0.0	0.0	0.169748304387924	2.8200095294629715E-4
76-77	1.0846190497934506E-4	0.0	0.0	0.17234054391693032	2.8200095294629715E-4
78-79	1.0846190497934506E-4	5.423095248967253E-6	0.0	0.17388070296763702	2.8200095294629715E-4
80-81	1.0846190497934506E-4	1.0846190497934505E-5	0.0	0.1744284355877827	3.036933339421662E-4
82-83	1.1388500022831231E-4	1.0846190497934505E-5	0.0	0.17476466749321867	3.253857149380352E-4
84-85	1.2473119072624683E-4	1.0846190497934505E-5	0.0	0.1749110910649408	3.253857149380352E-4
86-87	1.3015428597521408E-4	1.0846190497934505E-5	0.0	0.17502497606516912	3.253857149380352E-4
88	1.3015428597521408E-4	1.0846190497934505E-5	0.0	0.17503582225566705	3.253857149380352E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	12535	0.0	51.951363	1
GGTATCA	14290	0.0	49.30202	1
TATACAC	16525	0.0	41.558487	3
TTATACA	17320	0.0	38.966866	2
GTATCAA	25810	0.0	36.379105	1
ATCAACG	32245	0.0	28.686031	3
TCAACGC	32425	0.0	28.55335	4
TACACAT	24465	0.0	28.505125	5
TATCAAC	32820	0.0	28.286598	2
CAACGCA	32895	0.0	28.133894	5
AACGCAG	33695	0.0	27.491047	6
ACACATC	25450	0.0	26.188084	6
ATACACA	28195	0.0	25.468275	4
ACATCTC	26785	0.0	24.835709	8
ACGCAGA	38420	0.0	24.154043	7
CACATCT	27510	0.0	24.119267	7
CGCAGAG	38555	0.0	24.036932	8
CATCTCC	30985	0.0	21.209867	9
GCAGAGT	44460	0.0	20.758896	9
GTACATG	31865	0.0	20.330307	1
>>END_MODULE