FastQCFastQC Report
Fri 27 May 2016
SRR2139233_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139233_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10628580
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA166850.054.6518171
GTATCAA303250.038.1184461
TCAACGC375300.030.2834454
ATCAACG381100.029.7989753
TATCAAC386700.029.607132
CAACGCA386200.029.4184135
AACGCAG393250.028.9359116
ACGCAGA448450.025.3446437
GTACATG375700.023.437011
TACATGG376600.022.9825272
CGCAGAG498150.022.8340748
ACATGGG378850.022.3683743
GCAGAGT520150.021.6988839
ATACAAC119800.021.480026
TATATAC148050.021.4348753
GAGTACT307350.021.3040912-13
TATACAA140050.020.611885
TGGTATC71250.020.5042362
CATGGGG278400.020.1503664
CAGAGTA509750.019.80779310-11
Produced by FastQC (version 0.11.5)