##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139233_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10628580
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58868155482671	33.0	33.0	33.0	33.0	33.0
2	32.59391884899018	33.0	33.0	33.0	33.0	33.0
3	32.58597658388985	33.0	33.0	33.0	33.0	33.0
4	36.505533853064094	37.0	37.0	37.0	37.0	37.0
5	36.54189534255752	37.0	37.0	37.0	37.0	37.0
6	36.540877991227426	37.0	37.0	37.0	37.0	37.0
7	36.51955661057263	37.0	37.0	37.0	37.0	37.0
8	36.51464184303077	37.0	37.0	37.0	37.0	37.0
9	36.53296432825457	37.0	37.0	37.0	37.0	37.0
10-11	36.48766589704363	37.0	37.0	37.0	37.0	37.0
12-13	36.46968978922867	37.0	37.0	37.0	37.0	37.0
14-15	38.467557942829615	40.0	37.0	40.0	37.0	40.0
16-17	38.44168449595336	40.0	37.0	40.0	37.0	40.0
18-19	38.476552418102884	40.0	37.0	40.0	37.0	40.0
20-21	38.472208826424975	40.0	37.0	40.0	37.0	40.0
22-23	38.47185764747614	40.0	37.0	40.0	37.0	40.0
24-25	38.447109915880105	40.0	37.0	40.0	37.0	40.0
26-27	38.33591961619242	40.0	37.0	40.0	37.0	40.0
28-29	38.2836301597095	40.0	37.0	40.0	37.0	40.0
30-31	38.2027316653178	40.0	37.0	40.0	37.0	40.0
32-33	38.12226604426401	40.0	37.0	40.0	37.0	40.0
34-35	38.096568682959195	40.0	37.0	40.0	33.0	40.0
36-37	38.032240434844724	40.0	37.0	40.0	33.0	40.0
38-39	37.97424546768974	40.0	37.0	40.0	33.0	40.0
40-41	37.9279963106482	40.0	37.0	40.0	33.0	40.0
42-43	37.86166671019649	40.0	37.0	40.0	33.0	40.0
44-45	37.780282950154	40.0	37.0	40.0	33.0	40.0
46-47	37.721823451153575	40.0	37.0	40.0	33.0	40.0
48-49	37.67713523965213	40.0	37.0	40.0	33.0	40.0
50-51	37.58272059954182	40.0	37.0	40.0	33.0	40.0
52-53	37.46683642137786	40.0	37.0	40.0	33.0	40.0
54-55	37.27822112772489	40.0	37.0	40.0	33.0	40.0
56-57	37.15713457228347	37.0	37.0	40.0	33.0	40.0
58-59	37.01031789396487	37.0	37.0	40.0	33.0	40.0
60-61	36.86615191984287	37.0	37.0	40.0	33.0	40.0
62-63	36.69960884020615	37.0	37.0	40.0	33.0	40.0
64-65	36.49498104766711	37.0	37.0	40.0	33.0	40.0
66-67	36.273713575677945	37.0	37.0	40.0	33.0	40.0
68-69	36.057562903827105	37.0	37.0	40.0	33.0	40.0
70-71	35.80517334284215	37.0	33.0	37.0	33.0	40.0
72-73	35.621476300175985	37.0	33.0	37.0	33.0	40.0
74-75	35.43958927168559	37.0	33.0	37.0	33.0	40.0
76-77	33.60263586880867	35.0	33.0	37.0	27.0	37.0
78-79	34.93764516366329	37.0	33.0	37.0	33.0	37.0
80-81	35.0115758152893	37.0	33.0	37.0	33.0	37.0
82-83	34.882163935227055	37.0	33.0	37.0	33.0	37.0
84-85	34.74350082896433	37.0	33.0	37.0	33.0	37.0
86-87	34.65602811353382	37.0	33.0	37.0	33.0	37.0
88-89	34.548129923571665	37.0	33.0	37.0	33.0	37.0
90-91	34.491469362431765	37.0	33.0	37.0	33.0	37.0
92-93	34.419669440308915	37.0	33.0	37.0	33.0	37.0
94-95	34.43850110712191	37.0	33.0	37.0	33.0	37.0
96-97	34.43122975771752	37.0	33.0	37.0	33.0	37.0
98-99	34.446189220978766	37.0	33.0	37.0	33.0	37.0
100	34.45400405196136	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	2.0
19	10.0
20	66.0
21	417.0
22	1541.0
23	4514.0
24	10434.0
25	21000.0
26	36738.0
27	59042.0
28	87390.0
29	122690.0
30	166638.0
31	220873.0
32	293982.0
33	392760.0
34	539015.0
35	783170.0
36	1395595.0
37	3170026.0
38	3254249.0
39	68426.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.460968445455556	19.779678941119133	13.949304610775851	23.810048002649463
2	15.003791663608872	21.237606528811938	40.994328499197444	22.764273308381743
3	18.23661298122609	26.288459982424744	30.21255896836642	25.26236806798274
4	12.516224114686697	18.130626339503834	38.45226438539737	30.900885160412095
5	13.717655604041179	37.394826025677936	35.19888828046644	13.688630089814444
6	30.034829718813462	37.601324807003614	18.66827390747972	13.695571566703208
7	26.560311913726952	32.54706649430121	23.150872458973822	17.74174913299801
8	24.88162106320882	36.07569402497794	20.80005983866142	18.24262507315182
9	25.977647061037317	16.40332951344394	21.149899610296014	36.46912381522273
10-11	24.597547134074933	26.6366764680063	28.28035086857633	20.48542552934244
12-13	25.438955833684197	24.40456975652898	28.359568427564376	21.79690598222245
14-15	22.547856062332418	25.292314935577526	26.498701502027487	25.66112750006257
16-17	21.978241684213696	28.50032177393405	26.894787450440226	22.626649091412023
18-19	22.003983598937957	27.45020971757281	28.82819718156141	21.71760950192782
20-21	23.071065317613396	26.55102886944583	28.812926613884976	21.5649791990558
22-23	22.81807178386382	26.490183784855986	28.595302478163948	22.096441953116244
24-25	22.50333499793039	26.64829931575838	28.86150125823585	21.986864428075386
26-27	22.360467820808434	26.685145475309945	28.825609307120935	22.128777396760686
28-29	22.45451121293668	26.7170302306654	28.73957562856107	22.08888292783685
30-31	22.736577391942017	26.413801862848064	28.6939444831029	22.155676262107015
32-33	22.620425890445787	26.53430861740846	28.52494056019223	22.32032493195352
34-35	22.77037243998198	26.65788334981426	28.452114791946165	22.119629418257592
36-37	22.8990013823749	26.507493373712748	28.28800436934284	22.30550087456951
38-39	22.461581142552106	26.944114783375277	28.381428602567322	22.212875471505292
40-41	22.68592216606408	26.633409748910015	28.360146751064423	22.320521333961477
42-43	22.502221634313685	26.862062405471143	28.0050551207296	22.630660839485568
44-45	22.51053634712243	26.88956071731533	27.88943499987861	22.71046793568363
46-47	22.732501946160312	26.874875266288033	27.66202661288973	22.730596174661926
48-49	22.896321916242236	27.193039806585624	27.306772908366533	22.603865368805607
50-51	22.854846971693576	27.153462346082108	27.32142298533259	22.67026769689173
52-53	23.12136691741868	27.07831706634869	27.153361107430797	22.646954908801835
54-55	23.157367404888625	26.981181288131182	27.117619227414874	22.74383207956532
56-57	23.103956682304165	27.328277920257904	26.857119819423858	22.710645578014073
58-59	22.85873717287696	27.22670368250658	27.190114214498962	22.724444930117496
60-61	22.945697060762242	27.241378569962333	27.052882351845593	22.760042017429832
62-63	22.982123939774326	27.221260352366848	27.196386772574492	22.600228935284335
64-65	23.11605952727745	27.101975961593027	27.051180293096554	22.73078421803297
66-67	23.14700266963725	27.24937856205127	26.773321244792893	22.83029752351859
68-69	23.093823111572352	27.147823895275813	26.90844915869321	22.849903834458626
70-71	23.09039110679469	27.010511951933374	26.911747454058727	22.987349487213205
72-73	23.060347270138507	27.1010300857362	26.75245168499053	23.086170959134762
74-75	22.92075197238273	27.113191297810385	26.811479129447598	23.154577600359282
76-77	23.06056253333975	27.154162891419205	26.7219889842612	23.06328559097985
78-79	22.90326104113428	27.197677972022856	26.809547742596983	23.089513244245886
80-81	23.016887075727716	27.045837474620278	26.88660111878801	23.050674330864
82-83	23.223854475171436	26.89702250370602	27.07204422632691	22.807078794795636
84-85	23.145600232451528	26.91535924871008	26.99737706576858	22.941663453069815
86-87	22.940261492790086	27.13499725579005	26.932383224314687	22.992358027105176
88-89	23.220482859727827	26.901344157445433	26.76507852580283	23.113094457023912
90-91	23.201951646883465	26.899457543441624	26.87499832547572	23.02359248419919
92-93	23.207064756046115	26.94617110313325	27.01127148934093	22.8354926514797
94-95	22.92934072938971	27.143673773945725	27.04032048560367	22.8866650110609
96-97	22.958510094564797	27.179037268084905	27.083416231503467	22.779036405846835
98-99	23.306586527101523	27.969591866296568	26.10943806725954	22.614383539342374
100	21.177380235021552	27.857818926332538	27.370516744421003	23.594284094224907
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2888.0
1	2512.0
2	3403.5
3	4867.5
4	6410.5
5	10425.0
6	13995.0
7	16990.0
8	19463.0
9	19442.5
10	17187.0
11	14773.5
12	15669.5
13	20798.5
14	33081.5
15	49944.5
16	63612.0
17	71308.5
18	72036.5
19	66245.5
20	57290.0
21	48497.5
22	42585.5
23	42342.5
24	47632.0
25	58344.0
26	73665.5
27	90121.5
28	108344.5
29	128686.5
30	147171.0
31	164254.5
32	183761.5
33	204715.5
34	224919.5
35	242851.5
36	263339.0
37	281855.5
38	293819.0
39	307823.5
40	323459.0
41	335897.5
42	343973.0
43	351757.0
44	360633.5
45	376659.5
46	397531.5
47	419197.5
48	433607.0
49	434996.5
50	430908.0
51	429661.5
52	406282.5
53	368211.5
54	347908.0
55	333104.0
56	314988.5
57	288917.5
58	261522.5
59	232578.0
60	198546.5
61	173656.0
62	146085.0
63	120240.5
64	103720.0
65	82554.0
66	67305.5
67	49847.5
68	30139.5
69	21226.5
70	14936.5
71	8299.5
72	5281.0
73	3743.0
74	2948.0
75	2229.5
76	1387.5
77	700.5
78	558.5
79	490.0
80	326.5
81	206.5
82	167.5
83	106.5
84	60.0
85	44.5
86	34.5
87	28.5
88	21.0
89	10.5
90	4.0
91	1.5
92	1.0
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0010443539964887125
5	0.0
6	1.2231172931849785E-4
7	0.0
8	0.0
9	0.0
10-11	0.004149190202265966
12-13	4.704297281480687E-5
14-15	0.0013736548061923607
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	187.0
20-21	798.0
22-23	1504.0
24-25	3388.0
26-27	4223.0
28-29	6411.0
30-31	12159.0
32-33	15120.0
34-35	21335.0
36-37	27053.0
38-39	32051.0
40-41	37585.0
42-43	51173.0
44-45	63030.0
46-47	60734.0
48-49	51333.0
50-51	41398.0
52-53	38600.0
54-55	41296.0
56-57	45901.0
58-59	47760.0
60-61	48613.0
62-63	51113.0
64-65	56657.0
66-67	61291.0
68-69	65203.0
70-71	65234.0
72-73	63991.0
74-75	67858.0
76-77	65680.0
78-79	73848.0
80-81	77555.0
82-83	78317.0
84-85	81039.0
86-87	88203.0
88-89	96623.0
90-91	105550.0
92-93	111065.0
94-95	130247.0
96-97	375497.0
98-99	50625.0
100-101	8211332.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.80814411587089
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.51612086255531	70.72015307123344
2	8.334434840168829	13.469804233773958
3	2.184941526687523	5.296832093199488
4	0.8523459819268896	2.7550598777652633
5	0.41481002028392266	1.676001394990527
6	0.20522259634464832	0.9950194284750923
7	0.13063798291715312	0.738962906540319
8	0.08256714797300556	0.5337678394111255
9	0.0588486255880573	0.4279903395786595
>10	0.21192578186864583	2.7525139932471876
>50	0.006181344782632552	0.3366780651792226
>100	0.0018664030160511183	0.23466178619531516
>500	7.266453060042713E-5	0.04094656739808381
>1k	2.422135668327101E-5	0.021608403012242185
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.822578368888412E-5
2	0.0	3.7634378251845494E-5	0.0	0.0	2.822578368888412E-5
3	0.0	5.645156737776824E-5	0.0	0.0	2.822578368888412E-5
4	0.0	1.6935470213330474E-4	0.0	0.0	3.7634378251845494E-5
5	0.0	1.6935470213330474E-4	0.0	0.0	3.7634378251845494E-5
6	3.7634378251845494E-5	1.787632966962661E-4	0.0	9.408594562961373E-6	6.586016194072962E-5
7	3.7634378251845494E-5	1.8817189125922747E-4	0.0	9.408594562961373E-6	8.467735106665237E-5
8	3.7634378251845494E-5	2.446234586369957E-4	0.0	1.8817189125922747E-5	9.408594562961373E-5
9	3.7634378251845494E-5	4.1397816077030046E-4	0.0	2.822578368888412E-5	9.408594562961373E-5
10-11	3.7634378251845494E-5	4.1397816077030046E-4	0.0	3.7634378251845494E-5	9.408594562961373E-5
12-13	4.233867553332618E-5	4.1397816077030046E-4	0.0	3.7634378251845494E-5	1.0349454019257512E-4
14-15	4.704297281480687E-5	5.833328629036052E-4	0.0	3.7634378251845494E-5	1.2231172931849785E-4
16-17	7.526875650369099E-5	5.833328629036052E-4	0.0	4.233867553332618E-5	1.740589994147854E-4
18-19	7.526875650369099E-5	6.115586465924893E-4	0.0	4.704297281480687E-5	1.9287618854070817E-4
20-21	7.526875650369099E-5	6.586016194072962E-4	0.0	5.645156737776824E-5	2.163976749481116E-4
22-23	8.467735106665237E-5	7.620961595998714E-4	0.0	1.081988374740558E-4	2.2110197222959227E-4
24-25	8.467735106665237E-5	8.279563215406009E-4	0.0	3.9045667436289703E-4	2.2580626951107296E-4
26-27	8.467735106665237E-5	8.56182105229485E-4	4.704297281480687E-6	9.032250780442919E-4	2.3521486407403436E-4
28-29	8.467735106665237E-5	8.56182105229485E-4	9.408594562961373E-6	0.00301075026014764	2.3991916135551503E-4
30-31	8.467735106665237E-5	9.502680508590988E-4	9.408594562961373E-6	0.004563168363036266	2.540320531999571E-4
32-33	8.467735106665237E-5	9.549723481405794E-4	9.408594562961373E-6	0.00794555810842088	2.916664314518026E-4
34-35	8.467735106665237E-5	0.0010772840774590773	9.408594562961373E-6	0.012080635418842404	3.0107502601476395E-4
36-37	8.467735106665237E-5	0.0012043001040590558	9.408594562961373E-6	0.016860201456826783	3.0107502601476395E-4
38-39	8.467735106665237E-5	0.0012278215904664592	9.408594562961373E-6	0.02776005825801753	3.0107502601476395E-4
40-41	8.467735106665237E-5	0.0014395149681330902	9.408594562961373E-6	0.03891394711240824	3.1048362057772535E-4
42-43	8.467735106665237E-5	0.0014771493463849356	9.408594562961373E-6	0.0494468687256435	3.1989221514068674E-4
44-45	8.467735106665237E-5	0.0016512083457997213	9.408594562961373E-6	0.06295290622077455	3.575265933925322E-4
46-47	8.467735106665237E-5	0.0017970415615256225	9.408594562961373E-6	0.07721163128094252	3.622308906740129E-4
48-49	8.938164834813305E-5	0.0018017458588071031	9.408594562961373E-6	0.0884690146755258	3.7634378251845494E-4
50-51	1.0819883747405578E-4	0.0019616919663774466	9.408594562961373E-6	0.09823984012916119	3.7634378251845494E-4
52-53	1.2231172931849785E-4	0.0019758048582218884	9.408594562961373E-6	0.12039237602765374	3.7634378251845494E-4
54-55	1.2231172931849785E-4	0.001980509155503369	9.408594562961373E-6	0.1449535121342644	3.7634378251845494E-4
56-57	1.2231172931849785E-4	0.0020040306419107728	9.408594562961373E-6	0.16989099202339353	3.8104807979993564E-4
58-59	1.2231172931849785E-4	0.0020087349391922533	9.408594562961373E-6	0.18786611193593122	3.8575237708141633E-4
60-61	1.2701602659997855E-4	0.0020840036956959445	9.408594562961373E-6	0.2018331705646474	3.8575237708141633E-4
62-63	1.5994610757034337E-4	0.002187498235888519	9.408594562961373E-6	0.2129164949598159	3.8575237708141633E-4
64-65	1.5994610757034337E-4	0.0025120947483106867	1.8817189125922747E-5	0.21618598157044497	3.9045667436289703E-4
66-67	1.5994610757034337E-4	0.0025309119374366095	1.8817189125922747E-5	0.2192672962898148	3.951609716443777E-4
68-69	1.6465040485182407E-4	0.002587363504814378	1.8817189125922747E-5	0.22256030438685132	3.951609716443777E-4
70-71	1.740589994147854E-4	0.002639110774910665	1.8817189125922747E-5	0.22569336637631743	4.0927386348881976E-4
72-73	1.8817189125922747E-4	0.0026579279640365884	1.8817189125922747E-5	0.228360702934917	4.1397816077030046E-4
74-75	1.8817189125922747E-4	0.0027096752341328753	1.8817189125922747E-5	0.23041648084692407	4.1868245805178116E-4
76-77	1.8817189125922747E-4	0.002841395558014335	1.8817189125922747E-5	0.232491075948057	4.2338675533326185E-4
78-79	1.8817189125922747E-4	0.002926072909080987	1.8817189125922747E-5	0.2339541124025975	4.2338675533326185E-4
80-81	1.8817189125922747E-4	0.002926072909080987	1.8817189125922747E-5	0.23444335931987154	4.3279534989622325E-4
82-83	1.8817189125922747E-4	0.0029307772063624678	1.8817189125922747E-5	0.2347914773187011	4.516125390221459E-4
84-85	1.8817189125922747E-4	0.002954298692769871	1.8817189125922747E-5	0.23491849334530107	4.563168363036266E-4
86-87	1.8817189125922747E-4	0.0030107502601476395	1.8817189125922747E-5	0.23498905780452328	4.65725430866588E-4
88	1.8817189125922747E-4	0.0032365565296587125	1.8817189125922747E-5	0.23498905780452328	4.704297281480687E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16685	0.0	54.651817	1
GTATCAA	30325	0.0	38.118446	1
TCAACGC	37530	0.0	30.283445	4
ATCAACG	38110	0.0	29.798975	3
TATCAAC	38670	0.0	29.60713	2
CAACGCA	38620	0.0	29.418413	5
AACGCAG	39325	0.0	28.935911	6
ACGCAGA	44845	0.0	25.344643	7
GTACATG	37570	0.0	23.43701	1
TACATGG	37660	0.0	22.982527	2
CGCAGAG	49815	0.0	22.834074	8
ACATGGG	37885	0.0	22.368374	3
GCAGAGT	52015	0.0	21.698883	9
ATACAAC	11980	0.0	21.48002	6
TATATAC	14805	0.0	21.434875	3
GAGTACT	30735	0.0	21.30409	12-13
TATACAA	14005	0.0	20.61188	5
TGGTATC	7125	0.0	20.504236	2
CATGGGG	27840	0.0	20.150366	4
CAGAGTA	50975	0.0	19.807793	10-11
>>END_MODULE