FastQCFastQC Report
Fri 27 May 2016
SRR2139232_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139232_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10764140
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145200.054.7904241
GTATCAA267900.039.2600171
TCAACGC328200.031.3625344
ATCAACG333300.030.8948693
CAACGCA339750.030.3234375
TATCAAC344500.030.0440622
AACGCAG345250.029.8403686
ACGCAGA391100.026.3870647
CGCAGAG419050.024.6487068
GTACATG345300.022.8352451
TACATGG346350.022.3076442
GCAGAGT463050.022.2975279
ACATGGG350600.021.3328423
GAGTACT271100.020.76491412-13
CAGAGTA448950.020.06013710-11
AGAGTAC419600.019.88140110-11
CATGGGG262650.019.645244
GTGGTAT69200.019.628151
TGGTATC62450.019.489982
GTACTTT304450.018.52701814-15
Produced by FastQC (version 0.11.5)