##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139232_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10764140
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.590837911807164	33.0	33.0	33.0	33.0	33.0
2	32.59949638336179	33.0	33.0	33.0	33.0	33.0
3	32.594659303948106	33.0	33.0	33.0	33.0	33.0
4	36.51401895553198	37.0	37.0	37.0	37.0	37.0
5	36.548640671711816	37.0	37.0	37.0	37.0	37.0
6	36.54513727989417	37.0	37.0	37.0	37.0	37.0
7	36.52672884224843	37.0	37.0	37.0	37.0	37.0
8	36.52004656201053	37.0	37.0	37.0	37.0	37.0
9	36.53872617784607	37.0	37.0	37.0	37.0	37.0
10-11	36.493410899523795	37.0	37.0	37.0	37.0	37.0
12-13	36.47687367499866	37.0	37.0	37.0	37.0	37.0
14-15	38.48894514564099	40.0	37.0	40.0	37.0	40.0
16-17	38.46094722848272	40.0	37.0	40.0	37.0	40.0
18-19	38.48729722021453	40.0	37.0	40.0	37.0	40.0
20-21	38.48218663496925	40.0	37.0	40.0	37.0	40.0
22-23	38.48074277811428	40.0	37.0	40.0	37.0	40.0
24-25	38.457182958204115	40.0	37.0	40.0	37.0	40.0
26-27	38.34571036876979	40.0	37.0	40.0	37.0	40.0
28-29	38.29492431289913	40.0	37.0	40.0	37.0	40.0
30-31	38.21908345160594	40.0	37.0	40.0	37.0	40.0
32-33	38.14126595694943	40.0	37.0	40.0	37.0	40.0
34-35	38.12148524825488	40.0	37.0	40.0	35.0	40.0
36-37	38.061897833221906	40.0	37.0	40.0	33.0	40.0
38-39	38.00718946341418	40.0	37.0	40.0	33.0	40.0
40-41	37.96543397362228	40.0	37.0	40.0	33.0	40.0
42-43	37.90009430244497	40.0	37.0	40.0	33.0	40.0
44-45	37.820941432028896	40.0	37.0	40.0	33.0	40.0
46-47	37.768578803699846	40.0	37.0	40.0	33.0	40.0
48-49	37.73142611456291	40.0	37.0	40.0	33.0	40.0
50-51	37.64156531422998	40.0	37.0	40.0	33.0	40.0
52-53	37.52247848856486	40.0	37.0	40.0	33.0	40.0
54-55	37.33147679222759	40.0	37.0	40.0	33.0	40.0
56-57	37.20929115848692	38.5	37.0	40.0	33.0	40.0
58-59	37.067701142762616	37.0	37.0	40.0	33.0	40.0
60-61	36.92052431154753	37.0	37.0	40.0	33.0	40.0
62-63	36.747094369880685	37.0	37.0	40.0	33.0	40.0
64-65	36.5414968897648	37.0	37.0	40.0	33.0	40.0
66-67	36.32039839946842	37.0	37.0	40.0	33.0	40.0
68-69	36.09654797696561	37.0	37.0	40.0	33.0	40.0
70-71	35.83709775708266	37.0	35.0	37.0	33.0	40.0
72-73	35.64233537554705	37.0	33.0	37.0	33.0	40.0
74-75	35.45404087746377	37.0	33.0	37.0	33.0	40.0
76-77	33.62139044909813	35.0	33.0	37.0	27.0	37.0
78-79	34.946907125868314	37.0	33.0	37.0	33.0	37.0
80-81	35.017208485005426	37.0	33.0	37.0	33.0	37.0
82-83	34.890185000681655	37.0	33.0	37.0	33.0	37.0
84-85	34.75349966275924	37.0	33.0	37.0	33.0	37.0
86-87	34.66299234413263	37.0	33.0	37.0	33.0	37.0
88-89	34.54991949936165	37.0	33.0	37.0	33.0	37.0
90-91	34.49216692816693	37.0	33.0	37.0	33.0	37.0
92-93	34.42369455813079	37.0	33.0	37.0	33.0	37.0
94-95	34.443685575375405	37.0	33.0	37.0	33.0	37.0
96-97	34.43118825430524	37.0	33.0	37.0	33.0	37.0
98-99	34.44413259806161	37.0	33.0	37.0	33.0	37.0
100	34.45209465692735	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	12.0
20	64.0
21	354.0
22	1494.0
23	4152.0
24	10067.0
25	20200.0
26	36156.0
27	57508.0
28	85632.0
29	120627.0
30	164078.0
31	219355.0
32	292907.0
33	394507.0
34	543324.0
35	789354.0
36	1396042.0
37	3208742.0
38	3348621.0
39	70944.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.32352050419262	19.65383207576267	13.986068557265142	24.03657886277956
2	15.00546258224066	20.908990407036697	41.135873372141205	22.949673638581437
3	18.320163059937904	25.961953300495903	30.020614744884405	25.697268894681784
4	12.630598680581834	17.924417783113263	38.34991625315043	31.095067283154464
5	13.780134780855693	37.37199627652558	35.1584334651909	13.68943547742783
6	30.412954878842974	37.38806573313403	18.57435761180885	13.624621776214148
7	26.933633341818297	32.3601049410357	22.89774194687174	17.808519770274263
8	24.89613661658061	36.22979634229952	20.700176697813294	18.173890343306574
9	26.0005815606263	16.523791032075017	20.879940246039162	36.59568716125952
10-11	24.667969097485308	26.46983693694359	28.3600664249937	20.5021275405774
12-13	25.49710791943227	24.149517241814987	28.395998136961566	21.957376701791176
14-15	22.58960321282962	25.233776442789065	26.2688035301475	25.90781681423382
16-17	22.162239621558246	28.37092884336324	26.813744525805127	22.653087009273385
18-19	22.170475300395573	27.466862192427822	28.60107263562161	21.761589871554996
20-21	23.11393388552063	26.560723283632328	28.58725675817354	21.7380860726735
22-23	22.852315789556815	26.534979983637186	28.33312404802478	22.27958017878122
24-25	22.5732055559012	26.69324388188234	28.516422941262103	22.217127620954354
26-27	22.44596250093305	26.785457494361513	28.467108536804265	22.301471467901173
28-29	22.49752451124441	26.85312465824864	28.412259453540038	22.23709137696691
30-31	22.777060801887114	26.594231965277576	28.33930532348899	22.28940190934632
32-33	22.61619847347375	26.726842582098964	28.23462858706441	22.422330357362878
34-35	22.73454628038001	26.828020963406413	28.180025197962998	22.257407558250577
36-37	22.834449647490075	26.717720344182684	28.030990691179763	22.41683931714748
38-39	22.517639465599384	27.046882125541543	28.127259546725554	22.308218862133522
40-41	22.766838002892957	26.763221148645638	28.092954997762277	22.37698585069913
42-43	22.606874862934035	26.918516304741697	27.812985484994567	22.6616233473297
44-45	22.632166672922903	27.0009324557774	27.687382965505495	22.679517905794206
46-47	22.800745892739776	26.991475566102842	27.413028509364047	22.79475003179334
48-49	22.850689213742406	27.235861244870136	27.213802987163344	22.699646554224117
50-51	22.817767208757708	27.259911873023835	27.16420703809912	22.758113880119335
52-53	23.061468921190624	27.169390057086346	27.02292801076344	22.74621301095959
54-55	23.040880065488867	27.086545848382613	27.033171293709536	22.839402792418984
56-57	23.02734395892977	27.27868644584609	26.911938416928077	22.782031178296066
58-59	22.851896311903218	27.263735724902705	27.081750919900134	22.80261704329395
60-61	22.96185942664544	27.276190152927672	27.00107752937576	22.76087289105113
62-63	22.981850015701433	27.292002624113238	27.046725998181685	22.67942136200364
64-65	23.016723878013245	27.19571117609198	26.96114274356054	22.826422202334236
66-67	23.084260142656856	27.292044377673413	26.746379493247463	22.87731598642227
68-69	23.018153815434268	27.260422242189954	26.837630611485647	22.88379333089014
70-71	23.019281497950846	27.09016799408839	26.856964911277974	23.03358559668279
72-73	23.042517756754695	27.144000549256358	26.730993351735062	23.082488342253885
74-75	22.89511430702417	27.224711812100438	26.74061820874489	23.1395556721305
76-77	23.013679837143016	27.228057104627634	26.677603695977865	23.080659362251485
78-79	22.921829767561466	27.229217251498728	26.70700759618577	23.14194538475404
80-81	22.967674312141174	27.27346987808342	26.712463891653528	23.046391918121877
82-83	23.099154066365205	27.13732360960614	26.869879631863157	22.8936426921655
84-85	23.08748657487574	27.188373685528365	26.762818630680496	22.961321108915396
86-87	22.891770298266504	27.34663373568979	26.775023496297806	22.986572469745894
88-89	23.079711132708855	27.16836090086882	26.683313161255207	23.06861480516712
90-91	23.08945994847565	27.23117576588579	26.728704019464754	22.950660266173806
92-93	23.086918261362218	27.264289706835765	26.78611561518558	22.862676416616438
94-95	22.881950016565288	27.377559579732193	26.832446598946508	22.908043804756016
96-97	22.91429216201105	27.42466291494451	26.91619572284311	22.74484920020133
98-99	23.184393752056263	28.17310313346624	26.00942499285522	22.633078121622283
100	21.091771686445735	28.004354143428735	27.233204430626667	23.670669739498866
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3805.0
1	3071.5
2	3280.0
3	4459.5
4	5930.0
5	9523.0
6	12644.0
7	14831.5
8	16522.5
9	16587.5
10	15257.5
11	13781.0
12	14746.5
13	19236.0
14	29838.5
15	44683.5
16	56874.0
17	63833.5
18	65134.0
19	60970.0
20	53368.5
21	45359.0
22	39912.0
23	39680.0
24	44456.5
25	54098.5
26	67993.5
27	83285.5
28	100948.5
29	121387.5
30	141066.5
31	160391.0
32	181107.0
33	203331.5
34	224690.5
35	244364.5
36	266680.0
37	286721.0
38	302404.5
39	319266.0
40	335708.5
41	350257.0
42	361007.0
43	371231.0
44	383080.5
45	399239.0
46	415184.0
47	429268.0
48	440136.0
49	443708.0
50	444614.0
51	441047.5
52	420896.0
53	392918.0
54	372755.5
55	352300.5
56	330927.5
57	303646.0
58	271161.5
59	237490.0
60	201328.0
61	170828.5
62	140443.5
63	112320.0
64	91150.5
65	71086.5
66	55981.5
67	40877.5
68	26471.5
69	19172.0
70	13573.5
71	8196.5
72	5428.0
73	3797.0
74	2808.0
75	2036.0
76	1283.0
77	694.0
78	506.0
79	405.5
80	267.5
81	162.0
82	126.5
83	82.0
84	47.5
85	32.0
86	24.0
87	21.5
88	17.5
89	11.5
90	8.0
91	5.5
92	4.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0014213861952743088
5	0.0
6	9.290105851466072E-5
7	0.0
8	0.0
9	0.0
10-11	0.003906489510541483
12-13	3.251537048013125E-5
14-15	0.0015839630476749654
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	215.0
20-21	736.0
22-23	1567.0
24-25	2953.0
26-27	3915.0
28-29	6015.0
30-31	11770.0
32-33	14823.0
34-35	20633.0
36-37	27837.0
38-39	33143.0
40-41	38760.0
42-43	50597.0
44-45	62169.0
46-47	59934.0
48-49	52065.0
50-51	43039.0
52-53	41004.0
54-55	44574.0
56-57	48883.0
58-59	51341.0
60-61	52961.0
62-63	54176.0
64-65	59275.0
66-67	64192.0
68-69	67491.0
70-71	67854.0
72-73	66066.0
74-75	70526.0
76-77	68481.0
78-79	76052.0
80-81	79176.0
82-83	78589.0
84-85	80485.0
86-87	87700.0
88-89	95034.0
90-91	101233.0
92-93	108339.0
94-95	136424.0
96-97	386640.0
98-99	52845.0
100-101	8294628.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.84298562315054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.42171492622475	72.36697143481328
2	7.826314613980931	12.810579088685886
3	1.9482541779804234	4.783528160360842
4	0.7712809858708457	2.52495754552148
5	0.3568231869390369	1.460173747932918
6	0.1996364566640411	0.9803306191567097
7	0.11911618093733481	0.6824176718757224
8	0.08620260030059389	0.5644062541663758
9	0.055951590916874044	0.412132072590191
>10	0.2060082277414091	2.736020336327616
>50	0.006717865940408742	0.36919359398080254
>100	0.0018867380626383698	0.24527926069970746
>500	6.933664397271257E-5	0.037831321709715406
>1k	2.311179678774476E-5	0.026178892178788066
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.716042340586429E-5	0.0	0.0	0.0
3	0.0	3.716042340586429E-5	0.0	0.0	0.0
4	0.0	1.0219116436612679E-4	0.0	0.0	0.0
5	9.290105851466072E-6	1.30061481920525E-4	0.0	0.0	0.0
6	9.290105851466072E-6	1.30061481920525E-4	0.0	0.0	0.0
7	9.290105851466072E-6	1.30061481920525E-4	0.0	0.0	9.290105851466072E-6
8	9.290105851466072E-6	1.4864169362345715E-4	0.0	0.0	9.290105851466072E-6
9	9.290105851466072E-6	2.787031755439822E-4	0.0	0.0	1.8580211702932144E-5
10-11	9.290105851466072E-6	2.787031755439822E-4	0.0	0.0	1.8580211702932144E-5
12-13	9.290105851466072E-6	2.787031755439822E-4	0.0	0.0	1.8580211702932144E-5
14-15	9.290105851466072E-6	4.2734486916743927E-4	0.0	0.0	1.8580211702932144E-5
16-17	9.290105851466072E-6	4.4592508087037145E-4	0.0	0.0	1.8580211702932144E-5
18-19	9.290105851466072E-6	4.737953984247697E-4	0.0	2.7870317554398215E-5	2.7870317554398215E-5
20-21	9.290105851466072E-6	5.34181086459299E-4	0.0	4.1805476331597325E-5	2.7870317554398215E-5
22-23	9.290105851466072E-6	5.945667744938286E-4	0.0	6.967579388599554E-5	3.716042340586429E-5
24-25	9.290105851466072E-6	6.781777271570233E-4	0.0	9.754611144039376E-5	4.6450529257330355E-5
26-27	9.290105851466072E-6	7.153381505628875E-4	0.0	1.9973727580652054E-4	5.109558218306339E-5
28-29	9.290105851466072E-6	7.199832034886205E-4	0.0	6.54952462528358E-4	5.574063510879643E-5
30-31	9.290105851466072E-6	7.524985739687517E-4	0.0	0.0021088540282827983	5.574063510879643E-5
32-33	9.290105851466072E-6	7.617886798202179E-4	0.0	0.005332520758741525	5.574063510879643E-5
34-35	9.290105851466072E-6	8.453996324834126E-4	0.0	0.009276170692688872	7.432084681172857E-5
36-37	9.290105851466072E-6	9.336556380723402E-4	0.0	0.014325343222960682	7.432084681172857E-5
38-39	9.290105851466072E-6	9.754611144039376E-4	0.0	0.02662079831737603	7.432084681172857E-5
40-41	9.290105851466072E-6	0.0010915874375472634	0.0	0.03935753343973601	7.89658997374616E-5
42-43	9.290105851466072E-6	0.0011008775433987293	0.0	0.050435984667609304	8.825600558892768E-5
44-45	1.8580211702932144E-5	0.0011798434431361912	0.0	0.06454301040306054	9.290105851466071E-5
46-47	1.8580211702932144E-5	0.0012541642899479197	0.0	0.07879403277920949	9.290105851466071E-5
48-49	1.8580211702932144E-5	0.0012588093428736527	0.0	0.09249693891012195	1.0219116436612679E-4
50-51	1.8580211702932144E-5	0.0013842257718684446	0.0	0.10302262883983301	1.0219116436612679E-4
52-53	1.8580211702932144E-5	0.0014028059835713767	0.0	0.1296666524218377	1.0683621729185982E-4
54-55	1.8580211702932144E-5	0.0014120960894228429	0.0	0.16031935667875	1.1148127021759286E-4
56-57	1.8580211702932144E-5	0.001421386195274309	0.0	0.19194287699714052	1.1148127021759286E-4
58-59	1.8580211702932144E-5	0.001426031248200042	0.0	0.21197699026582711	1.1148127021759286E-4
60-61	1.8580211702932144E-5	0.0014678367245316393	0.0	0.22456972874748934	1.1148127021759286E-4
62-63	1.8580211702932144E-5	0.0015235773596404358	0.0	0.2334789402590453	1.2077137606905894E-4
64-65	1.8580211702932144E-5	0.0017279596883726894	0.0	0.2369348596357907	1.2077137606905894E-4
66-67	1.8580211702932144E-5	0.0017326047412984224	0.0	0.24046509985934778	1.2077137606905894E-4
68-69	1.8580211702932144E-5	0.001797635482258685	0.0	0.2438652786009844	1.2077137606905894E-4
70-71	1.8580211702932144E-5	0.00182086074688735	0.0	0.2470610750138887	1.2077137606905894E-4
72-73	1.8580211702932144E-5	0.0018347959056645492	0.0	0.249927072669066	1.2077137606905894E-4
74-75	1.8580211702932144E-5	0.0018812464349218794	0.0	0.25218456839097225	1.2077137606905894E-4
76-77	1.8580211702932144E-5	0.001955567281733608	0.0	0.2544559992716557	1.2077137606905894E-4
78-79	1.8580211702932144E-5	0.001978792546362273	0.0	0.25601209200177627	1.2077137606905894E-4
80-81	1.8580211702932144E-5	0.001978792546362273	0.0	0.25649982255897824	1.2541642899479197E-4
82-83	1.8580211702932144E-5	0.001983437599288006	0.0	0.2569178773222942	1.30061481920525E-4
84-85	1.8580211702932144E-5	0.0019927277051394725	0.0	0.25708045417469483	1.30061481920525E-4
86-87	1.8580211702932144E-5	0.0020345331814710698	0.0	0.25714548491565514	1.30061481920525E-4
88	1.8580211702932144E-5	0.002173884769243061	0.0	0.2571594200744323	1.30061481920525E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14520	0.0	54.790424	1
GTATCAA	26790	0.0	39.260017	1
TCAACGC	32820	0.0	31.362534	4
ATCAACG	33330	0.0	30.894869	3
CAACGCA	33975	0.0	30.323437	5
TATCAAC	34450	0.0	30.044062	2
AACGCAG	34525	0.0	29.840368	6
ACGCAGA	39110	0.0	26.387064	7
CGCAGAG	41905	0.0	24.648706	8
GTACATG	34530	0.0	22.835245	1
TACATGG	34635	0.0	22.307644	2
GCAGAGT	46305	0.0	22.297527	9
ACATGGG	35060	0.0	21.332842	3
GAGTACT	27110	0.0	20.764914	12-13
CAGAGTA	44895	0.0	20.060137	10-11
AGAGTAC	41960	0.0	19.881401	10-11
CATGGGG	26265	0.0	19.64524	4
GTGGTAT	6920	0.0	19.62815	1
TGGTATC	6245	0.0	19.48998	2
GTACTTT	30445	0.0	18.527018	14-15
>>END_MODULE