FastQCFastQC Report
Fri 27 May 2016
SRR2139231_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139231_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10418702
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140150.054.658531
GTATCAA264600.040.681361
TCAACGC330850.031.9488334
ATCAACG334400.031.6089083
CAACGCA338000.031.2869575
TATCAAC341950.031.078862
AACGCAG346000.030.652896
ACGCAGA395850.026.725547
CGCAGAG400700.026.4023158
GTACATG319050.023.4287851
TACATGG319950.022.7832972
GCAGAGT469400.022.3788819
AGACCGA45350.021.43879188-89
ACATGGG331300.021.4297073
CGAGACC46550.021.24229686-87
GAGACCG45050.020.98702688-89
GAGTACT294600.020.93754212-13
AGAGTAC422500.020.22255510-11
CAGAGTA455200.019.81260710-11
GTGGTAT65700.019.4215581
Produced by FastQC (version 0.11.5)