##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139231_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10418702
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57240182126334	33.0	33.0	33.0	33.0	33.0
2	32.583781165830445	33.0	33.0	33.0	33.0	33.0
3	32.578225675328845	33.0	33.0	33.0	33.0	33.0
4	36.49513931773843	37.0	37.0	37.0	37.0	37.0
5	36.53020971326371	37.0	37.0	37.0	37.0	37.0
6	36.52749344400099	37.0	37.0	37.0	37.0	37.0
7	36.506663114080816	37.0	37.0	37.0	37.0	37.0
8	36.50042942009475	37.0	37.0	37.0	37.0	37.0
9	36.52070065925679	37.0	37.0	37.0	37.0	37.0
10-11	36.472913948397796	37.0	37.0	37.0	37.0	37.0
12-13	36.45564869788962	37.0	37.0	37.0	37.0	37.0
14-15	38.4431713278679	40.0	37.0	40.0	37.0	40.0
16-17	38.415010718225744	40.0	37.0	40.0	37.0	40.0
18-19	38.44753108400643	40.0	37.0	40.0	37.0	40.0
20-21	38.44345357934851	40.0	37.0	40.0	37.0	40.0
22-23	38.444706093295196	40.0	37.0	40.0	37.0	40.0
24-25	38.4213633949597	40.0	37.0	40.0	37.0	40.0
26-27	38.30460437385295	40.0	37.0	40.0	37.0	40.0
28-29	38.25163574809943	40.0	37.0	40.0	37.0	40.0
30-31	38.17359043856881	40.0	37.0	40.0	37.0	40.0
32-33	38.09745926624878	40.0	37.0	40.0	33.0	40.0
34-35	38.078672187848596	40.0	37.0	40.0	33.0	40.0
36-37	38.019268645627335	40.0	37.0	40.0	33.0	40.0
38-39	37.97050678164681	40.0	37.0	40.0	33.0	40.0
40-41	37.93542174443003	40.0	37.0	40.0	33.0	40.0
42-43	37.871677444981316	40.0	37.0	40.0	33.0	40.0
44-45	37.79705059769179	40.0	37.0	40.0	33.0	40.0
46-47	37.74858394524844	40.0	37.0	40.0	33.0	40.0
48-49	37.72229557122535	40.0	37.0	40.0	33.0	40.0
50-51	37.64465647181003	40.0	37.0	40.0	33.0	40.0
52-53	37.53573850304448	40.0	37.0	40.0	33.0	40.0
54-55	37.35000817047506	40.0	37.0	40.0	33.0	40.0
56-57	37.23381596990122	38.5	37.0	40.0	33.0	40.0
58-59	37.10947751621592	37.0	37.0	40.0	33.0	40.0
60-61	36.97340180520317	37.0	37.0	40.0	33.0	40.0
62-63	36.80282710751975	37.0	37.0	40.0	33.0	40.0
64-65	36.60563946340854	37.0	37.0	40.0	33.0	40.0
66-67	36.39375379677411	37.0	37.0	40.0	33.0	40.0
68-69	36.168837308315695	37.0	37.0	40.0	33.0	40.0
70-71	35.905254668053196	37.0	35.0	37.0	33.0	40.0
72-73	35.70575025013949	37.0	33.0	37.0	33.0	40.0
74-75	35.508672897319684	37.0	33.0	37.0	33.0	40.0
76-77	33.69463856481029	35.0	33.0	37.0	27.0	37.0
78-79	34.98782103192285	37.0	33.0	37.0	33.0	37.0
80-81	35.04127764046768	37.0	33.0	37.0	33.0	37.0
82-83	34.90984908565619	37.0	33.0	37.0	33.0	37.0
84-85	34.770776358179084	37.0	33.0	37.0	33.0	37.0
86-87	34.669137731010586	37.0	33.0	37.0	33.0	37.0
88-89	34.548883394069094	37.0	33.0	37.0	33.0	37.0
90-91	34.488156788312565	37.0	33.0	37.0	33.0	37.0
92-93	34.42570559481135	37.0	33.0	37.0	33.0	37.0
94-95	34.44138591946883	37.0	33.0	37.0	33.0	37.0
96-97	34.42513372783051	37.0	33.0	37.0	33.0	37.0
98-99	34.443750837100424	37.0	33.0	37.0	33.0	37.0
100	34.45820497833497	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	19.0
20	65.0
21	380.0
22	1459.0
23	4330.0
24	10088.0
25	20292.0
26	36218.0
27	57005.0
28	85520.0
29	119425.0
30	162778.0
31	217573.0
32	287925.0
33	387336.0
34	529126.0
35	760834.0
36	1311518.0
37	3039977.0
38	3320819.0
39	66012.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.15340836123348	19.910599228195604	14.160525946514257	23.775466464056656
2	14.568158298413755	21.129071548452004	41.73170515866564	22.5710649944686
3	17.92474724778576	26.591633007643374	30.47381526028866	25.009804484282206
4	12.25010490881092	18.159926331554843	39.32185716046306	30.26811159917117
5	13.236591275957407	37.90557595370325	35.60635480312231	13.25147796721703
6	29.608338476334357	38.24895452307344	18.923636272890356	13.219070727701851
7	26.192264641027265	32.85647290804555	23.446010837050526	17.50525161387666
8	24.390091971149573	36.64478550207118	21.241724736920204	17.723397789859042
9	25.89598013264992	16.591366179779403	21.060944060018226	36.451709627552454
10-11	24.112258345659413	26.714931145130343	29.069631388644396	20.10317912056585
12-13	25.148322261733448	24.389167975771255	29.09602244963599	21.366487312859313
14-15	22.24458991362425	25.558131943325314	26.900751726560003	25.296526416490433
16-17	21.69049944993148	28.620978889692783	27.464649627180044	22.22387203319569
18-19	21.691756804254503	27.72578100419803	29.367890549129825	21.214571642417646
20-21	22.521453945782476	26.935371159632965	29.28943218509637	21.253742709488186
22-23	22.21948271265203	26.84026688700067	29.173620298694004	21.7666301016533
24-25	22.017133732809356	26.996896061505392	29.188543165137805	21.797427040547447
26-27	21.955604583189533	27.163283390166132	29.08508362034652	21.796028406297822
28-29	21.938762078656975	27.22471057917984	29.064919886841196	21.771607455321988
30-31	22.18462204700864	27.094711578169267	28.95899461882313	21.76167175599896
32-33	22.01316564974982	27.23468168974494	28.920215045844888	21.83193761466035
34-35	22.140265711668043	27.225549491046934	28.911759905232852	21.722424892052164
36-37	22.156937616458176	27.197857229977046	28.795814932488977	21.849390221075804
38-39	22.0029841998392	27.375246154836375	28.84114993543468	21.78061970988975
40-41	22.28648543594976	27.19955785535554	28.671846075358935	21.84211063333576
42-43	22.112239730182385	27.365224473168247	28.45242885166114	22.07010694498823
44-45	22.265312717349335	27.44300410388884	28.246928260085646	22.044754918676173
46-47	22.349266249405673	27.48952943686137	27.931390307937665	22.229814005795287
48-49	22.247759554062753	27.64980518508161	27.846841459629328	22.255593801226308
50-51	22.31158422038832	27.7642348437098	27.700581503675526	22.223599432226354
52-53	22.481110044649668	27.66054648952181	27.576453865094685	22.28188960073384
54-55	22.443779425274013	27.552495030264275	27.64927885107824	22.354446693383473
56-57	22.409925206199503	27.647866015524674	27.62402247272816	22.318186305547666
58-59	22.400517717623558	27.658410211563133	27.572329737908994	22.368742332904315
60-61	22.52504884568098	27.593490724859134	27.680145696610648	22.201314732849237
62-63	22.377663341490887	27.686479165954864	27.616180060497577	22.319677432056668
64-65	22.350686302502467	27.669945226601335	27.583533052079233	22.39583541881696
66-67	22.43859371146449	27.69885826088496	27.51500556731213	22.347542460338417
68-69	22.362809837968694	27.776863724206137	27.50075835852926	22.35956807929591
70-71	22.406432564268243	27.534775547197597	27.492157115523458	22.566634773010698
72-73	22.445887879011277	27.5570610104159	27.36229739549873	22.63475371507409
74-75	22.314143886230212	27.72606764423527	27.46099709537439	22.498791374160128
76-77	22.484461078245594	27.49706591901066	27.418304019756366	22.600168982987377
78-79	22.43288294507653	27.524359493122674	27.272789371662583	22.76996819013822
80-81	22.549565280194184	27.63930789464224	27.27223237644134	22.538894448722235
82-83	22.56531466429826	27.4988880318322	27.347554277006335	22.588243026863207
84-85	22.55385284997045	27.49486713903474	27.2963803375522	22.65489967344261
86-87	22.41482732536807	27.563582572514083	27.4026417159279	22.618948386189945
88-89	22.572532218746943	27.547857496878432	27.334118548804927	22.545491735569698
90-91	22.620060565772953	27.507729806200892	27.378558807320164	22.493650820705994
92-93	22.57029279931685	27.3455091275334	27.40746175237556	22.67673632077419
94-95	22.545891598461957	27.447449973535225	27.460685273992276	22.545973154010547
96-97	22.69644466063112	27.467561171662958	27.523974977638265	22.31201919006766
98-99	22.729669709462872	28.31415572318004	26.631567012106338	22.32460755525075
100	20.797585285870067	28.069523428949733	27.829836311964524	23.30305497321568
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2055.0
1	1922.0
2	3076.0
3	4651.0
4	6377.0
5	10463.5
6	13949.5
7	16595.0
8	18622.0
9	18399.5
10	16454.5
11	14586.0
12	15615.0
13	20305.0
14	31371.0
15	46760.5
16	59481.0
17	66772.5
18	67518.5
19	62434.0
20	54687.0
21	47119.5
22	42061.5
23	42160.0
24	47469.5
25	58097.5
26	73799.5
27	91346.5
28	110375.0
29	132551.5
30	153555.0
31	174241.0
32	197212.0
33	219591.5
34	242177.5
35	263318.5
36	286571.0
37	308747.5
38	324420.5
39	338272.5
40	353244.0
41	367765.5
42	377515.0
43	385715.5
44	392748.5
45	400174.0
46	405929.5
47	406946.5
48	407992.0
49	405782.5
50	399420.0
51	389665.5
52	376449.5
53	360367.5
54	341547.5
55	319030.0
56	293977.5
57	266208.5
58	236291.0
59	204535.5
60	169639.0
61	137098.5
62	108420.0
63	82427.0
64	59853.0
65	42791.0
66	31484.5
67	23815.0
68	17848.0
69	12652.0
70	8703.0
71	6094.0
72	4241.5
73	2806.0
74	1810.0
75	1200.5
76	783.0
77	515.5
78	349.5
79	230.5
80	163.5
81	132.0
82	105.0
83	80.0
84	59.0
85	44.0
86	35.5
87	29.0
88	19.5
89	11.5
90	5.0
91	3.0
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.001247756198420878
5	0.0
6	1.1517749523885031E-4
7	0.0
8	0.0
9	0.0
10-11	0.00395442733653386
12-13	2.8794373809712577E-5
14-15	0.0014685130642953413
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	160.0
20-21	722.0
22-23	1436.0
24-25	3189.0
26-27	3788.0
28-29	6276.0
30-31	11770.0
32-33	14429.0
34-35	19914.0
36-37	25525.0
38-39	31757.0
40-41	37611.0
42-43	48233.0
44-45	59578.0
46-47	57521.0
48-49	49500.0
50-51	41254.0
52-53	38026.0
54-55	40228.0
56-57	44617.0
58-59	30355.0
60-61	50142.0
62-63	49716.0
64-65	53697.0
66-67	59318.0
68-69	63170.0
70-71	65643.0
72-73	64576.0
74-75	67057.0
76-77	65668.0
78-79	72982.0
80-81	77800.0
82-83	77411.0
84-85	79260.0
86-87	85558.0
88-89	94663.0
90-91	103093.0
92-93	110608.0
94-95	129447.0
96-97	384580.0
98-99	50432.0
100-101	8047992.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.72063262495664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.50818372329606	73.2145295007694
2	7.860548054206832	13.00459015645722
3	1.9552793967316826	4.852258459685651
4	0.733152915225345	2.42587491833087
5	0.35364893327282204	1.4627031743734458
6	0.18014093869716172	0.8940823446409649
7	0.11015610832273734	0.637852807757206
8	0.0760689107937921	0.5033974739163101
9	0.04894554454375669	0.36439257679492937
>10	0.1688410976464557	2.2250175640321985
>50	0.004108719916577096	0.23232964026554379
>100	8.193899553535604E-4	0.11064350794354945
>500	8.501430002426869E-5	0.0485587799859465
>1k	2.1253091332508957E-5	0.023769095046730387
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	3.83924984129501E-5	0.0	0.0	0.0
5	0.0	3.83924984129501E-5	0.0	9.598124603237525E-6	0.0
6	4.7990623016187623E-5	3.83924984129501E-5	0.0	9.598124603237525E-6	0.0
7	4.7990623016187623E-5	3.83924984129501E-5	0.0	1.919624920647505E-5	0.0
8	4.7990623016187623E-5	3.83924984129501E-5	0.0	2.8794373809712573E-5	0.0
9	4.7990623016187623E-5	4.7990623016187623E-5	0.0	2.8794373809712573E-5	9.598124603237525E-6
10-11	4.7990623016187623E-5	4.7990623016187623E-5	0.0	3.3593436111331335E-5	9.598124603237525E-6
12-13	7.198593452428144E-5	5.278968531780638E-5	0.0	3.83924984129501E-5	9.598124603237525E-6
14-15	7.67849968259002E-5	1.1037843293723154E-4	0.0	3.83924984129501E-5	1.919624920647505E-5
16-17	1.2477561984208782E-4	1.151774952388503E-4	0.0	3.83924984129501E-5	3.83924984129501E-5
18-19	1.3437374444532534E-4	1.2957468214370657E-4	0.0	5.278968531780638E-5	3.83924984129501E-5
20-21	1.5356999365180037E-4	1.4397186904856286E-4	0.0	5.758874761942515E-5	3.83924984129501E-5
22-23	1.6316811825503792E-4	2.0635967896960676E-4	0.0	8.638312142913772E-5	3.83924984129501E-5
24-25	1.8236436746151295E-4	2.4955123968417565E-4	0.0	4.0312123333597597E-4	3.83924984129501E-5
26-27	1.8236436746151295E-4	2.783456134938882E-4	0.0	7.774480928622394E-4	4.7990623016187623E-5
28-29	1.8236436746151295E-4	2.8794373809712577E-4	0.0	0.002317947091681862	4.7990623016187623E-5
30-31	1.919624920647505E-4	3.551306103197884E-4	0.0	0.0031097923714489576	4.7990623016187623E-5
32-33	2.1115874127122553E-4	3.551306103197884E-4	0.0	0.004669487619475056	4.7990623016187623E-5
34-35	2.399531150809381E-4	4.4151373174892614E-4	0.0	0.006598710664725798	4.7990623016187623E-5
36-37	2.399531150809381E-4	5.278968531780638E-4	0.0	0.009142213684583742	4.7990623016187623E-5
38-39	2.399531150809381E-4	5.374949777813014E-4	0.0	0.01678232086876081	4.7990623016187623E-5
40-41	2.687474888906507E-4	6.430743484169141E-4	0.0	0.024288054308492554	4.7990623016187623E-5
42-43	2.783456134938882E-4	6.718687222266268E-4	0.0	0.03079078372718598	4.7990623016187623E-5
44-45	2.783456134938882E-4	7.582518436557644E-4	0.0	0.038550867468903516	4.7990623016187623E-5
46-47	2.783456134938882E-4	8.254387158784271E-4	0.0	0.04718438054951567	4.7990623016187623E-5
48-49	3.0234092500198203E-4	8.254387158784271E-4	0.0	0.055275599590044903	4.7990623016187623E-5
50-51	3.167381119068383E-4	9.502143357205149E-4	0.0	0.06327083738454176	4.7990623016187623E-5
52-53	3.167381119068383E-4	9.502143357205149E-4	0.0	0.0843051274525368	5.278968531780638E-5
54-55	3.2633623651007583E-4	9.598124603237524E-4	0.0	0.10951940078524178	5.758874761942515E-5
56-57	3.359343611133133E-4	9.982049587367026E-4	0.0	0.13585665469652553	5.758874761942515E-5
58-59	3.4553248571655087E-4	9.982049587367026E-4	0.0	0.23791351360275015	6.238780992104391E-5
60-61	3.5033154801816964E-4	9.982049587367026E-4	0.0	0.32814068393548446	6.718687222266267E-5
62-63	3.7432685952626344E-4	0.00100780308333994	0.0	0.3328773584271822	6.718687222266267E-5
64-65	3.7432685952626344E-4	0.0011229805785787903	0.0	0.33657743546173025	6.718687222266267E-5
66-67	3.887240464311197E-4	0.0011325787031820278	0.0	0.3400375593811974	6.718687222266267E-5
68-69	4.1271935793921356E-4	0.0011373777654836467	0.0	0.3434976833006645	6.718687222266267E-5
70-71	4.271165448440698E-4	0.001151774952388503	0.0	0.3466602653574313	6.718687222266267E-5
72-73	4.6550904325701995E-4	0.0011613730769917405	0.0	0.3492805533741151	7.67849968259002E-5
74-75	4.895043547651138E-4	0.0011997655754046905	0.0	0.3515793042165905	7.67849968259002E-5
76-77	4.943034170667325E-4	0.0012477561984208783	0.0	0.3537676766261286	7.67849968259002E-5
78-79	5.0390154166997E-4	0.0012669524476273532	0.0	0.35539455874637743	7.67849968259002E-5
80-81	5.087006039715887E-4	0.0012669524476273532	0.0	0.3559080584126506	7.67849968259002E-5
82-83	5.134996662732076E-4	0.0012669524476273532	0.0	0.35644555339043194	7.67849968259002E-5
84-85	5.182987285748263E-4	0.001271751509928972	0.0	0.3566375158824967	7.67849968259002E-5
86-87	5.182987285748263E-4	0.0013341393198500159	0.0	0.3567190999416242	8.158405912751896E-5
88	5.182987285748263E-4	0.0013821299428662035	0.0	0.3567238990039258	8.638312142913772E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14015	0.0	54.65853	1
GTATCAA	26460	0.0	40.68136	1
TCAACGC	33085	0.0	31.948833	4
ATCAACG	33440	0.0	31.608908	3
CAACGCA	33800	0.0	31.286957	5
TATCAAC	34195	0.0	31.07886	2
AACGCAG	34600	0.0	30.65289	6
ACGCAGA	39585	0.0	26.72554	7
CGCAGAG	40070	0.0	26.402315	8
GTACATG	31905	0.0	23.428785	1
TACATGG	31995	0.0	22.783297	2
GCAGAGT	46940	0.0	22.378881	9
AGACCGA	4535	0.0	21.438791	88-89
ACATGGG	33130	0.0	21.429707	3
CGAGACC	4655	0.0	21.242296	86-87
GAGACCG	4505	0.0	20.987026	88-89
GAGTACT	29460	0.0	20.937542	12-13
AGAGTAC	42250	0.0	20.222555	10-11
CAGAGTA	45520	0.0	19.812607	10-11
GTGGTAT	6570	0.0	19.421558	1
>>END_MODULE