FastQCFastQC Report
Fri 27 May 2016
SRR2139230_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139230_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7711012
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105100.056.4423641
GTATCAA200550.040.4372831
TCAACGC247150.032.331154
ATCAACG250850.031.8534413
CAACGCA254150.031.425525
TATCAAC257000.031.2630622
AACGCAG259900.030.7982526
TATCTCG43850.029.21358994
ACGCAGA295500.027.0430227
ATCTCGT46950.025.68752994
CGCAGAG325350.024.6027898
GTACATG280600.024.1788391
TACATGG285100.023.5803012
ACATGGG288450.022.6939243
GCAGAGT352450.022.5863239
GTGGTAT47150.022.2960111
TGGTATC45150.021.3270442
GAGTACT198200.021.19421212-13
CATGGGG214850.020.3742434
CGCTATC69550.020.3054294
Produced by FastQC (version 0.11.5)