##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139230_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7711012
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59024418584746	33.0	33.0	33.0	33.0	33.0
2	32.60095471255913	33.0	33.0	33.0	33.0	33.0
3	32.59656112582888	33.0	33.0	33.0	33.0	33.0
4	36.516765892725886	37.0	37.0	37.0	37.0	37.0
5	36.550978003924776	37.0	37.0	37.0	37.0	37.0
6	36.548278358275155	37.0	37.0	37.0	37.0	37.0
7	36.5268994523676	37.0	37.0	37.0	37.0	37.0
8	36.52182126029631	37.0	37.0	37.0	37.0	37.0
9	36.53853696505725	37.0	37.0	37.0	37.0	37.0
10-11	36.494034829669566	37.0	37.0	37.0	37.0	37.0
12-13	36.47568425519245	37.0	37.0	37.0	37.0	37.0
14-15	38.48847842539993	40.0	37.0	40.0	37.0	40.0
16-17	38.45938548662614	40.0	37.0	40.0	37.0	40.0
18-19	38.491634431382025	40.0	37.0	40.0	37.0	40.0
20-21	38.487294895502856	40.0	37.0	40.0	37.0	40.0
22-23	38.48698762725448	40.0	37.0	40.0	37.0	40.0
24-25	38.46264510160006	40.0	37.0	40.0	37.0	40.0
26-27	38.349333383338006	40.0	37.0	40.0	37.0	40.0
28-29	38.2981680293377	40.0	37.0	40.0	37.0	40.0
30-31	38.22069079905795	40.0	37.0	40.0	37.0	40.0
32-33	38.14128629483926	40.0	37.0	40.0	37.0	40.0
34-35	38.11940755099657	40.0	37.0	40.0	35.0	40.0
36-37	38.05607122099352	40.0	37.0	40.0	33.0	40.0
38-39	37.999907955756115	40.0	37.0	40.0	33.0	40.0
40-41	37.95683242762462	40.0	37.0	40.0	33.0	40.0
42-43	37.891448423366484	40.0	37.0	40.0	33.0	40.0
44-45	37.81243866574201	40.0	37.0	40.0	33.0	40.0
46-47	37.754827085545834	40.0	37.0	40.0	33.0	40.0
48-49	37.714555680236515	40.0	37.0	40.0	33.0	40.0
50-51	37.6203234297221	40.0	37.0	40.0	33.0	40.0
52-53	37.50231716896554	40.0	37.0	40.0	33.0	40.0
54-55	37.314612762160486	40.0	37.0	40.0	33.0	40.0
56-57	37.19256771799209	38.5	37.0	40.0	33.0	40.0
58-59	37.05057208771845	37.0	37.0	40.0	33.0	40.0
60-61	36.904391577849196	37.0	37.0	40.0	33.0	40.0
62-63	36.738861067740224	37.0	37.0	40.0	33.0	40.0
64-65	36.53828709318151	37.0	37.0	40.0	33.0	40.0
66-67	36.31789278533829	37.0	37.0	40.0	33.0	40.0
68-69	36.09635477192086	37.0	37.0	40.0	33.0	40.0
70-71	35.83656720609638	37.0	35.0	37.0	33.0	40.0
72-73	35.649520227606295	37.0	33.0	37.0	33.0	40.0
74-75	35.458734871137096	37.0	33.0	37.0	33.0	40.0
76-77	33.60952761214496	35.0	33.0	37.0	27.0	37.0
78-79	34.933209889603106	37.0	33.0	37.0	33.0	37.0
80-81	34.99798174646286	37.0	33.0	37.0	33.0	37.0
82-83	34.87222316061771	37.0	33.0	37.0	33.0	37.0
84-85	34.736474353019666	37.0	33.0	37.0	33.0	37.0
86-87	34.645536722460704	37.0	33.0	37.0	33.0	37.0
88-89	34.53371344621672	37.0	33.0	37.0	33.0	37.0
90-91	34.48457928124887	37.0	33.0	37.0	33.0	37.0
92-93	34.42455159119261	37.0	33.0	37.0	33.0	37.0
94-95	34.43282113794851	37.0	33.0	37.0	33.0	37.0
96-97	34.416754061109486	37.0	33.0	37.0	33.0	37.0
98-99	34.41896164089174	37.0	33.0	37.0	33.0	37.0
100	34.39575749569663	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	2.0
19	10.0
20	56.0
21	282.0
22	1103.0
23	3176.0
24	7472.0
25	15004.0
26	26420.0
27	42347.0
28	62793.0
29	88535.0
30	120517.0
31	159715.0
32	211064.0
33	281561.0
34	386053.0
35	562559.0
36	995010.0
37	2289013.0
38	2406535.0
39	51784.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.33257580198293	19.66787238821571	14.118639680498488	23.88091212930287
2	14.997849828271567	20.917565683985448	41.17678717138555	22.90779731635744
3	18.28508372182536	26.020579399954247	30.118549938711027	25.575786939509364
4	12.569503802988727	18.021001155635478	38.24367529171982	31.165819749655977
5	13.769022794932754	37.431779382524624	35.07626495718072	13.7229328653619
6	30.391013983524584	37.34420801029386	18.60240490115229	13.662373105029268
7	26.96967661313457	32.458216898119204	22.798447207707625	17.773659281038597
8	25.07004268700399	36.11195262048613	20.576235648446666	18.241769044063215
9	26.161624440475517	16.445480826641173	20.841492660107388	36.55140207277592
10-11	24.71983358158192	26.535281884813088	28.196911169370814	20.547973364234178
12-13	25.59770862716372	24.21661856121192	28.23591668044026	21.949756131184103
14-15	22.670414526781542	25.215575013464726	26.2772943560723	25.83671610368143
16-17	22.139791767928777	28.391604111107593	26.76762142245402	22.700982698509613
18-19	22.17918348460617	27.448569655967336	28.577876678184396	21.794370181242098
20-21	23.246231308277284	26.51052437591803	28.56539804216598	21.6778462736387
22-23	22.95877104769153	26.463177195514003	28.334182546366737	22.24386921042773
24-25	22.628635039126866	26.63868159284656	28.573762725215264	22.15892064281131
26-27	22.48342450797454	26.71169242510055	28.50286487542501	22.3020181914999
28-29	22.53642650916361	26.78942319606663	28.441136042474728	22.233014252295032
30-31	22.873784727781686	26.444164981804004	28.40601480219538	22.27603548821893
32-33	22.66742246051349	26.605352500632957	28.2469864227003	22.480238616153258
34-35	22.832090999602336	26.733302262776785	28.206782695979815	22.22782404164106
36-37	22.95538627580657	26.587572110738122	28.03636383339055	22.420677780064754
38-39	22.52268989964405	26.952678156809505	28.111013501380295	22.413618442166147
40-41	22.811953974703155	26.65011716365498	28.144495277352956	22.393433584288914
42-43	22.586769125322927	26.835767385458954	27.81556782833342	22.761895660884697
44-45	22.61179268144415	26.89001958191891	27.682817305102596	22.815370431534344
46-47	22.77689691022836	26.84286699297064	27.530014796885837	22.850221299915155
48-49	22.869190174304997	27.18498883894891	27.22160443646919	22.724216550276903
50-51	22.863504473688646	27.19276795794227	27.152718755792204	22.79100881257688
52-53	23.05077264959713	27.07057973679265	27.020762099741464	22.85788551386876
54-55	23.1182845155003	26.853030013619676	27.139174518590025	22.889510952289996
56-57	23.045464781006142	27.009008978502724	26.956170752396158	22.989355488094976
58-59	22.738744331998124	26.845601631899036	27.28178459765434	23.133869438448503
60-61	22.904867627474236	26.930609611131544	27.307910966524485	22.856611794869732
62-63	22.72648441728468	27.0418327716552	27.39205224873768	22.839630562322437
64-65	22.74966173148556	27.072264131521166	27.18158215270651	22.996491984286756
66-67	22.83844599103651	27.453193748378208	26.75275341194322	22.955606848642056
68-69	22.800601065906974	27.419349655630594	26.722423377020753	23.05762590144168
70-71	22.796803407896316	27.09631621865411	26.677234223539724	23.42964614990985
72-73	22.845545024583927	26.95605612181511	26.602089534627115	23.596309318973844
74-75	22.868011388524184	26.905727748085877	26.72611650996646	23.500144353423476
76-77	23.05479050781376	26.91785355478849	26.408798372267718	23.61855756513003
78-79	23.048615612933084	26.957981683177085	26.27721185057277	23.71619085331707
80-81	23.099002880917677	27.0146362449726	26.35595982705708	23.530401047052646
82-83	23.177746377413904	26.819390648232805	26.558368658742694	23.444494315610598
84-85	23.181680854572942	27.012631774845786	26.45729625618817	23.34839111439311
86-87	23.236608174648836	27.134262526437798	26.39155749132015	23.237571807593216
88-89	23.35438821693423	26.96603037916746	26.485604101844956	23.193977302053355
90-91	23.29293008133686	26.81876406183501	26.530224386648243	23.358081470179883
92-93	23.299579674357517	26.617597500554126	26.75810092491593	23.324721900172428
94-95	22.79214706514993	26.98829617811125	27.033337917553396	23.18621883918543
96-97	22.85518256544244	26.90291937004787	27.129222010438475	23.11267605407122
98-99	24.129776360052215	28.778541986443702	27.37424659758404	19.717435055920046
100	22.892495309878715	30.019752617345812	21.156466047122972	25.931286025652494
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1716.0
1	1480.0
2	1921.0
3	2781.5
4	3852.5
5	6545.0
6	8855.5
7	10415.5
8	11627.5
9	11698.0
10	10685.5
11	9547.5
12	10389.5
13	13790.0
14	21626.5
15	32680.0
16	42145.0
17	47907.0
18	48810.0
19	45208.5
20	39420.0
21	33178.5
22	28600.0
23	27707.5
24	30584.5
25	37180.0
26	46767.5
27	57389.5
28	69172.0
29	83029.0
30	96701.5
31	109965.5
32	124836.0
33	140850.0
34	156268.0
35	170857.0
36	188433.0
37	204792.0
38	216716.5
39	228925.0
40	241332.5
41	251566.5
42	257942.0
43	264034.0
44	272766.5
45	286163.5
46	298741.5
47	312256.5
48	321281.0
49	320607.5
50	319806.5
51	319334.0
52	302876.5
53	278480.5
54	263899.0
55	250308.5
56	233758.5
57	212744.5
58	191280.5
59	168184.0
60	142804.0
61	122887.5
62	101854.0
63	83126.5
64	70346.0
65	55676.0
66	44699.5
67	32958.0
68	20304.5
69	14267.0
70	10222.5
71	6074.0
72	3953.5
73	2832.0
74	2269.5
75	1771.0
76	1146.5
77	577.0
78	426.5
79	374.5
80	249.5
81	151.5
82	123.5
83	83.5
84	50.5
85	33.0
86	25.0
87	21.5
88	16.5
89	9.5
90	5.5
91	3.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0013357520387726023
5	0.0
6	1.1671619756265455E-4
7	0.0
8	0.0
9	0.0
10-11	0.004240688511443115
12-13	1.9452699593775756E-5
14-15	0.0015951213666896124
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	154.0
20-21	538.0
22-23	1136.0
24-25	2359.0
26-27	2619.0
28-29	4521.0
30-31	8891.0
32-33	11211.0
34-35	15642.0
36-37	20523.0
38-39	18857.0
40-41	29160.0
42-43	37791.0
44-45	46556.0
46-47	45268.0
48-49	38919.0
50-51	22353.0
52-53	9988.0
54-55	9101.0
56-57	9462.0
58-59	24595.0
60-61	41072.0
62-63	42238.0
64-65	46971.0
66-67	50758.0
68-69	53332.0
70-71	53246.0
72-73	51823.0
74-75	55398.0
76-77	52854.0
78-79	59149.0
80-81	61732.0
82-83	61411.0
84-85	63031.0
86-87	68381.0
88-89	74557.0
90-91	79236.0
92-93	83882.0
94-95	98137.0
96-97	279708.0
98-99	531629.0
100-101	5442823.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.78395419102617
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.04726535885004	74.60732001662043
2	7.716144935007846	12.929782675320316
3	1.7975309799649448	4.51812759847
4	0.636942075842007	2.1346210281873534
5	0.29506905216904533	1.2361025975060402
6	0.1568760176751765	0.7886215847140561
7	0.08724995198807844	0.5117102186376878
8	0.06251524587453781	0.41902195972744105
9	0.04111360550285686	0.31001943960713524
>10	0.15377063064248508	2.0994414812617306
>50	0.004321814636530664	0.24614530152813216
>100	0.00114521726402044	0.15646456469230607
>500	2.7557291580455595E-5	0.016947023998677417
>1k	2.755729078255199E-5	0.02567450972872185
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2968466395850506E-5
2	0.0	1.2968466395850506E-5	0.0	0.0	1.2968466395850506E-5
3	0.0	1.2968466395850506E-5	0.0	0.0	1.2968466395850506E-5
4	0.0	1.5562159675020607E-4	0.0	1.2968466395850506E-5	1.2968466395850506E-5
5	0.0	1.6859006314605656E-4	0.0	1.2968466395850506E-5	1.2968466395850506E-5
6	5.187386558340202E-5	1.6859006314605656E-4	0.0	1.2968466395850506E-5	3.890539918755152E-5
7	5.187386558340202E-5	1.6859006314605656E-4	0.0	1.2968466395850506E-5	3.890539918755152E-5
8	5.187386558340202E-5	1.6859006314605656E-4	0.0	1.2968466395850506E-5	5.187386558340202E-5
9	5.187386558340202E-5	2.853062607087111E-4	0.0	1.2968466395850506E-5	5.187386558340202E-5
10-11	5.187386558340202E-5	2.853062607087111E-4	0.0	5.8358098781327274E-5	6.484233197925253E-5
12-13	5.187386558340202E-5	2.853062607087111E-4	0.0	6.484233197925253E-5	8.429503157302828E-5
14-15	5.187386558340202E-5	4.085066914692909E-4	0.0	7.132656517717779E-5	1.232004307605798E-4
16-17	7.781079837510304E-5	4.149909246672162E-4	0.0	1.1023196436472929E-4	1.2968466395850507E-4
18-19	7.781079837510304E-5	4.5389632385476773E-4	0.0	1.361688971564303E-4	1.2968466395850507E-4
20-21	7.781079837510304E-5	4.992859562402445E-4	0.0	1.4913736355228081E-4	1.4265313035435556E-4
22-23	7.781079837510304E-5	5.770967546153475E-4	0.0	2.074954623336081E-4	1.4265313035435556E-4
24-25	7.781079837510304E-5	6.484233197925253E-4	0.0	3.6311705908381416E-4	1.4265313035435556E-4
26-27	7.781079837510304E-5	6.743602525842263E-4	0.0	0.0010698984776576666	1.4265313035435556E-4
28-29	7.781079837510304E-5	6.938129521780021E-4	0.0	0.0037089813892132446	1.4265313035435556E-4
30-31	7.781079837510304E-5	8.559187821261334E-4	0.0	0.006315643134779197	1.4913736355228081E-4
32-33	7.781079837510304E-5	8.818557149178344E-4	0.0	0.012864718664683701	2.0101122913568283E-4
34-35	7.781079837510304E-5	0.0010115403788763394	0.0	0.02063931426899608	2.1397969553153335E-4
36-37	7.781079837510304E-5	0.001154193509230695	0.0	0.03233687095805324	2.2694816192738386E-4
38-39	7.781079837510304E-5	0.001180130442022396	0.0	0.09253649196759128	2.334323951253091E-4
40-41	7.781079837510304E-5	0.0012968466395850507	0.0	0.15887668181556455	2.334323951253091E-4
42-43	7.781079837510304E-5	0.0013357520387726023	0.0	0.1857214072549751	2.3991662832323436E-4
44-45	8.429503157302828E-5	0.0014719209359290325	0.0	0.22279176844751375	2.464008615211596E-4
46-47	9.077926477095354E-5	0.0015691844338979113	0.0	0.26098390198329346	2.5936932791701013E-4
48-49	9.077926477095354E-5	0.0015756686670958366	0.0	0.29743177678883137	2.723377943128606E-4
50-51	9.077926477095354E-5	0.0018285537618149214	0.0	0.329042413628717	2.723377943128606E-4
52-53	9.077926477095354E-5	0.001893396093794174	0.0	0.5676168056799807	3.0475896030248686E-4
54-55	9.077926477095354E-5	0.0019128487933879495	0.0	0.895077066408404	3.3718012629211313E-4
56-57	9.077926477095354E-5	0.0019582384257734262	0.0	1.269573954754577	3.3718012629211313E-4
58-59	9.077926477095354E-5	0.001977691125367202	0.0	1.673548167218518	3.5014859268796364E-4
60-61	9.72634979688788E-5	0.002023080757752679	0.0	1.7876187976364193	3.5014859268796364E-4
62-63	1.0374773116680404E-4	0.002094407322929857	0.0	1.7929877427243013	3.5014859268796364E-4
64-65	1.0374773116680404E-4	0.0023213554848572405	0.0	1.7972608524017342	3.5014859268796364E-4
66-67	1.0374773116680404E-4	0.002360260884044792	0.0	1.800736401395822	3.5014859268796364E-4
68-69	1.0374773116680404E-4	0.0024445559156178204	0.0	1.804192497690316	3.5014859268796364E-4
70-71	1.0374773116680404E-4	0.0024899455480032967	0.0	1.807518909320852	3.6311705908381416E-4
72-73	1.5562159675020607E-4	0.0025029140143991474	0.0	1.810521109291491	3.760855254796647E-4
74-75	1.5562159675020607E-4	0.0025418194135866995	0.0	1.8130823814046715	3.760855254796647E-4
76-77	1.5562159675020607E-4	0.0026261144451597274	0.0	1.815299989158362	3.8256975867758997E-4
78-79	1.5562159675020607E-4	0.002658535611149354	0.0	1.8169145632246453	3.890539918755152E-4
80-81	1.5562159675020607E-4	0.002658535611149354	0.0	1.8174073649476878	3.9553822507344043E-4
82-83	1.5562159675020607E-4	0.0026715040775452045	0.0	1.8178418085719488	4.0202245827136566E-4
84-85	1.5562159675020607E-4	0.00269095677713898	0.0	1.81808820943347	4.0202245827136566E-4
86-87	1.5562159675020607E-4	0.002742830642722382	0.0	1.8181465675322512	4.0202245827136566E-4
88	1.5562159675020607E-4	0.0029049364726705133	0.0	1.818166020231845	4.0202245827136566E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10510	0.0	56.442364	1
GTATCAA	20055	0.0	40.437283	1
TCAACGC	24715	0.0	32.33115	4
ATCAACG	25085	0.0	31.853441	3
CAACGCA	25415	0.0	31.42552	5
TATCAAC	25700	0.0	31.263062	2
AACGCAG	25990	0.0	30.798252	6
TATCTCG	4385	0.0	29.213589	94
ACGCAGA	29550	0.0	27.043022	7
ATCTCGT	4695	0.0	25.687529	94
CGCAGAG	32535	0.0	24.602789	8
GTACATG	28060	0.0	24.178839	1
TACATGG	28510	0.0	23.580301	2
ACATGGG	28845	0.0	22.693924	3
GCAGAGT	35245	0.0	22.586323	9
GTGGTAT	4715	0.0	22.296011	1
TGGTATC	4515	0.0	21.327044	2
GAGTACT	19820	0.0	21.194212	12-13
CATGGGG	21485	0.0	20.374243	4
CGCTATC	6955	0.0	20.30542	94
>>END_MODULE