FastQCFastQC Report
Fri 27 May 2016
SRR2139229_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139229_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9204082
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123050.052.1393851
GTATCAA228150.039.3958631
TCAACGC275350.031.9932484
ATCAACG280400.031.4161973
CAACGCA282050.031.234625
AACGCAG287600.030.6318656
TATCAAC290850.030.5276952
ACGCAGA331150.026.6034227
CGCAGAG337350.026.1403878
GCAGAGT392950.022.2086229
GTACATG261900.021.5286331
GAGTACT245150.021.00404412-13
TACATGG264350.020.6186852
CAGAGTA379500.019.80160910-11
AGAGTAC352250.019.74011410-11
ACATGGG270200.019.5095673
GTGGTAT60300.019.4107881
GTACTTT273400.018.89020214-15
TGGTATC57750.018.8309882
AGTACTT266000.018.40538812-13
Produced by FastQC (version 0.11.5)