##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139229_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9204082
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57565632292281	33.0	33.0	33.0	33.0	33.0
2	32.58688699209764	33.0	33.0	33.0	33.0	33.0
3	32.580250806109724	33.0	33.0	33.0	33.0	33.0
4	36.49570397134663	37.0	37.0	37.0	37.0	37.0
5	36.53093094998502	37.0	37.0	37.0	37.0	37.0
6	36.52735634037159	37.0	37.0	37.0	37.0	37.0
7	36.50908477347333	37.0	37.0	37.0	37.0	37.0
8	36.502850148445006	37.0	37.0	37.0	37.0	37.0
9	36.52144602796889	37.0	37.0	37.0	37.0	37.0
10-11	36.47486626042662	37.0	37.0	37.0	37.0	37.0
12-13	36.457792368646864	37.0	37.0	37.0	37.0	37.0
14-15	38.4478779632776	40.0	37.0	40.0	37.0	40.0
16-17	38.417333906846984	40.0	37.0	40.0	37.0	40.0
18-19	38.448427176115985	40.0	37.0	40.0	37.0	40.0
20-21	38.444941592987334	40.0	37.0	40.0	37.0	40.0
22-23	38.447516120263415	40.0	37.0	40.0	37.0	40.0
24-25	38.423143332160265	40.0	37.0	40.0	37.0	40.0
26-27	38.30637498604107	40.0	37.0	40.0	37.0	40.0
28-29	38.25495156745737	40.0	37.0	40.0	37.0	40.0
30-31	38.177327644064576	40.0	37.0	40.0	37.0	40.0
32-33	38.10168300218605	40.0	37.0	40.0	33.0	40.0
34-35	38.0829087717647	40.0	37.0	40.0	33.0	40.0
36-37	38.0253018605129	40.0	37.0	40.0	33.0	40.0
38-39	37.975448868416926	40.0	37.0	40.0	33.0	40.0
40-41	37.94343308458653	40.0	37.0	40.0	33.0	40.0
42-43	37.88041747765758	40.0	37.0	40.0	33.0	40.0
44-45	37.80597797781978	40.0	37.0	40.0	33.0	40.0
46-47	37.75934394864252	40.0	37.0	40.0	33.0	40.0
48-49	37.73434926942514	40.0	37.0	40.0	33.0	40.0
50-51	37.65781197063126	40.0	37.0	40.0	33.0	40.0
52-53	37.549749026665204	40.0	37.0	40.0	33.0	40.0
54-55	37.36666062696767	40.0	37.0	40.0	33.0	40.0
56-57	37.25367263703912	40.0	37.0	40.0	33.0	40.0
58-59	37.13470761208163	37.0	37.0	40.0	33.0	40.0
60-61	36.998935622751404	37.0	37.0	40.0	33.0	40.0
62-63	36.83594143389318	37.0	37.0	40.0	33.0	40.0
64-65	36.641037341474586	37.0	37.0	40.0	33.0	40.0
66-67	36.430727874252966	37.0	37.0	40.0	33.0	40.0
68-69	36.210684497583784	37.0	37.0	40.0	33.0	40.0
70-71	35.95224720407293	37.0	37.0	38.5	33.0	40.0
72-73	35.75600354852983	37.0	33.0	37.0	33.0	40.0
74-75	35.56121541354469	37.0	33.0	37.0	33.0	40.0
76-77	33.7451771378131	35.0	33.0	37.0	27.0	37.0
78-79	35.03163304458921	37.0	33.0	37.0	33.0	37.0
80-81	35.08669674421779	37.0	33.0	37.0	33.0	37.0
82-83	34.95297827647752	37.0	33.0	37.0	33.0	37.0
84-85	34.80966727383879	37.0	33.0	37.0	33.0	37.0
86-87	34.711472237292256	37.0	33.0	37.0	33.0	37.0
88-89	34.5940971614109	37.0	33.0	37.0	33.0	37.0
90-91	34.53505490452885	37.0	33.0	37.0	33.0	37.0
92-93	34.47579557730015	37.0	33.0	37.0	33.0	37.0
94-95	34.49289201550377	37.0	33.0	37.0	33.0	37.0
96-97	34.471080288597335	37.0	33.0	37.0	33.0	37.0
98-99	34.46801468454473	37.0	33.0	37.0	33.0	37.0
100	34.45058511200006	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	2.0
18	4.0
19	19.0
20	68.0
21	390.0
22	1327.0
23	3893.0
24	8878.0
25	17638.0
26	31529.0
27	49678.0
28	73724.0
29	103809.0
30	141216.0
31	187993.0
32	249948.0
33	335116.0
34	460018.0
35	663892.0
36	1135830.0
37	2635135.0
38	3031611.0
39	72362.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.014423600311254	20.018867715433217	14.256402756950667	23.710305927304866
2	14.650673472922124	21.11623951199044	41.529508320330045	22.703578694757393
3	17.987627663464973	26.352253271972153	30.60162871213012	25.058490352432756
4	12.397952836579492	18.155705889479034	39.18485042571373	30.261490848227744
5	13.318938271084505	37.77599982268737	35.61601254747622	13.289049358751912
6	29.725254034466943	38.118696671666626	18.821062343533825	13.334986950332603
7	26.198799619560102	32.893437933299595	23.29847778409623	17.609284663044072
8	24.36258173275727	36.60240097817468	21.222496713958	17.812520575110042
9	25.61956749190196	16.790517511686662	21.217281636560823	36.37263335985055
10-11	24.030341099695097	26.75448291785222	29.040159175330537	20.175016807122145
12-13	25.029659399437023	24.4400078856329	29.080911450089992	21.449421264840087
14-15	22.20519573181474	25.7067000030476	26.840065608726942	25.248038656410714
16-17	21.612329181769567	28.818626344267685	27.46165777314891	22.107386700813837
18-19	21.63382507891607	27.878070838569236	29.35091734297891	21.137186739535785
20-21	22.40046697823901	27.029929306795903	29.328792120258306	21.24081159470678
22-23	22.135381196799443	26.963988987391456	29.198347837070983	21.702281978738114
24-25	21.94959814369012	27.101836203476775	29.205079855016546	21.743485797816554
26-27	21.853371214806305	27.27760145565054	29.118001107219847	21.751026222323308
28-29	21.80596461435694	27.342573414003713	29.166635623003362	21.684826348635983
30-31	22.15573744171833	27.166222291885383	29.02066655672949	21.657373709666796
32-33	21.898579113993723	27.35331451781846	28.972196142041952	21.775910226145864
34-35	22.02420043478503	27.334469264087414	28.980777287149735	21.660553013977825
36-37	22.01450455097046	27.34536071896167	28.885174356960803	21.754960373107068
38-39	21.84233836322128	27.44269711080619	28.96368542548961	21.751279100482922
40-41	22.138050110737137	27.275301186332758	28.800409858803334	21.786238844126775
42-43	22.012557771047653	27.430727585143373	28.57534214437143	21.981372499437548
44-45	22.100952619066813	27.495012253713597	28.381938441744666	22.022096685474928
46-47	22.158876354969088	27.534664005292885	28.142353559863665	22.16410607987436
48-49	22.072842886308187	27.70899420046312	28.07120187573683	22.146961037491863
50-51	22.11647778525664	27.809587962614447	27.92896466160827	22.144969590520645
52-53	22.29404393638307	27.67416808363892	27.80294183926413	22.22884614071388
54-55	22.264364645227317	27.57672269255893	27.868350826237787	22.290561835975968
56-57	22.258028066954516	27.691205797167417	27.825388631351185	22.225377504526882
58-59	22.181034024836883	27.7840340705573	27.829852753077418	22.205079151528402
60-61	22.2988235344737	27.725268376092426	27.821880222590995	22.154027866842874
62-63	22.233343669025743	27.81751296801837	27.747970779529386	22.201172583426498
64-65	22.223837656756093	27.824314391761725	27.66575785720706	22.286090094275124
66-67	22.292452267078524	27.794483049761926	27.557348471947247	22.355716211212307
68-69	22.25759657826263	27.767599438867556	27.64895898465844	22.325844998211373
70-71	22.243280725396417	27.616936838875905	27.670361555198546	22.46942088052913
72-73	22.352653365561256	27.601733814879832	27.559660470048307	22.485952349510605
74-75	22.211980005932972	27.745809318776928	27.554962530077553	22.48724814521255
76-77	22.354700705333418	27.637584585178253	27.494318453961125	22.513396255527205
78-79	22.349309051614068	27.669556921282084	27.453179441696495	22.527954585407347
80-81	22.34281965876933	27.703730731463473	27.4644165554769	22.489033054290303
82-83	22.386189363933326	27.65780686706044	27.532570259054573	22.423433509951664
84-85	22.478618267488127	27.632821263168932	27.49759227686834	22.390968192474602
86-87	22.327646225495734	27.63084375961157	27.57980817986644	22.46170183502626
88-89	22.397001250132263	27.58488032302268	27.516096922480042	22.502021504365015
90-91	22.430518361783786	27.58953156935366	27.56705555027443	22.412894518588132
92-93	22.474142419019483	27.530637839076967	27.592854839763607	22.402364902139947
94-95	22.356588109520732	27.547060834561847	27.632957396988616	22.4633936589288
96-97	22.416319534681232	27.66431582824986	27.711780955219766	22.20758368184914
98-99	23.548823726522453	29.544847066898537	27.890247974246506	19.0160812323325
100	22.43667389112523	30.562304040751982	21.80540853810315	25.19561353001964
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2242.0
1	1959.5
2	2689.5
3	3967.0
4	5310.0
5	8472.0
6	11331.5
7	13837.0
8	16243.5
9	16832.0
10	15158.0
11	12955.0
12	13319.0
13	17066.5
14	26202.5
15	38700.0
16	48706.0
17	54522.5
18	55772.0
19	52632.5
20	47457.0
21	42234.5
22	39067.5
23	40132.5
24	46179.0
25	57368.0
26	72177.5
27	88422.0
28	106254.0
29	126614.5
30	146111.5
31	164956.0
32	185774.0
33	205546.5
34	223554.0
35	240921.5
36	261454.5
37	281364.5
38	295126.0
39	305938.0
40	318610.5
41	330564.5
42	338380.5
43	346677.0
44	353477.0
45	358933.0
46	361502.5
47	362413.0
48	364708.0
49	362292.0
50	355240.5
51	345983.5
52	333430.5
53	317432.0
54	300228.0
55	280396.0
56	258360.5
57	233449.0
58	206311.5
59	178829.0
60	149404.5
61	120906.0
62	94867.5
63	72127.5
64	53315.0
65	39065.5
66	28958.5
67	21560.0
68	15990.0
69	11158.5
70	7522.5
71	5324.5
72	3707.0
73	2493.5
74	1664.0
75	1085.0
76	731.0
77	481.0
78	328.5
79	231.0
80	161.0
81	112.5
82	86.0
83	61.0
84	43.0
85	31.0
86	22.0
87	16.5
88	10.0
89	6.5
90	5.0
91	3.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0013798225613374587
5	0.0
6	1.7383591323936489E-4
7	0.0
8	0.0
9	0.0
10-11	0.00428614173580809
12-13	1.6297116866190455E-5
14-15	0.0013417959553163477
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	133.0
20-21	583.0
22-23	1250.0
24-25	3401.0
26-27	3432.0
28-29	5714.0
30-31	11628.0
32-33	14423.0
34-35	20446.0
36-37	27289.0
38-39	31575.0
40-41	37162.0
42-43	46082.0
44-45	57014.0
46-47	55993.0
48-49	49651.0
50-51	42016.0
52-53	40454.0
54-55	43756.0
56-57	49238.0
58-59	50169.0
60-61	51570.0
62-63	52765.0
64-65	57380.0
66-67	62601.0
68-69	64962.0
70-71	64622.0
72-73	64347.0
74-75	67156.0
76-77	66096.0
78-79	72244.0
80-81	76102.0
82-83	75318.0
84-85	77687.0
86-87	82689.0
88-89	89538.0
90-91	94947.0
92-93	100663.0
94-95	116675.0
96-97	320744.0
98-99	590999.0
100-101	6363568.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.4475618611973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.23271145473993	75.35484920616521
2	7.610805445813817	12.854279273977994
3	1.780650108854469	4.511146806619066
4	0.6386730664703254	2.1573753315933355
5	0.27661630507898777	1.1679786267486825
6	0.15845149413034945	0.8028505411543096
7	0.08618063526951464	0.5094421169711735
8	0.050579502919773894	0.3417052561380972
9	0.03792809520669516	0.2882641649620294
>10	0.12327821251387854	1.679679268910104
>50	0.0032890698213122543	0.18769398145696203
>100	7.49057511369762E-4	0.09375732874856808
>500	8.755166959901304E-5	0.05097809655445845
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.2594233732380916E-5
2	0.0	1.0864744577460305E-5	0.0	0.0	3.2594233732380916E-5
3	0.0	1.0864744577460305E-5	0.0	0.0	3.2594233732380916E-5
4	0.0	9.778270119714274E-5	0.0	1.0864744577460305E-5	3.2594233732380916E-5
5	0.0	9.778270119714274E-5	0.0	1.0864744577460305E-5	3.2594233732380916E-5
6	3.2594233732380916E-5	9.778270119714274E-5	0.0	1.0864744577460305E-5	3.2594233732380916E-5
7	3.2594233732380916E-5	9.778270119714274E-5	0.0	1.0864744577460305E-5	3.2594233732380916E-5
8	3.2594233732380916E-5	1.0864744577460305E-4	0.0	1.0864744577460305E-5	3.2594233732380916E-5
9	3.2594233732380916E-5	2.064301469717458E-4	0.0	1.0864744577460305E-5	4.345897830984122E-5
10-11	3.2594233732380916E-5	2.064301469717458E-4	0.0	1.0864744577460305E-5	4.345897830984122E-5
12-13	3.2594233732380916E-5	2.064301469717458E-4	0.0	2.172948915492061E-5	4.889135059857138E-5
14-15	3.2594233732380916E-5	2.770509867252378E-4	0.0	3.2594233732380916E-5	7.605321204222213E-5
16-17	6.518846746476183E-5	2.8248335901396795E-4	0.0	4.345897830984122E-5	8.691795661968244E-5
18-19	6.518846746476183E-5	2.933481035914282E-4	0.0	5.432372288730153E-5	8.691795661968244E-5
20-21	6.518846746476183E-5	3.1507759274634884E-4	0.0	9.778270119714274E-5	9.778270119714274E-5
22-23	6.518846746476183E-5	3.4767182647872977E-4	0.0	2.987804758801584E-4	1.1951219035206335E-4
24-25	6.518846746476183E-5	3.965631770773011E-4	0.0	7.713968649996816E-4	1.4124167950698398E-4
26-27	6.518846746476183E-5	4.237250385209519E-4	0.0	0.001673170664928887	1.4124167950698398E-4
28-29	6.518846746476183E-5	4.2915741080968206E-4	0.0	0.005970177145314438	1.4124167950698398E-4
30-31	6.518846746476183E-5	5.052106228519042E-4	0.0	0.009425165920946815	1.4124167950698398E-4
32-33	6.518846746476183E-5	5.106429951406343E-4	0.0	0.016193901792704586	1.5210642408444426E-4
34-35	6.518846746476183E-5	5.758314626053962E-4	0.0	0.025016074389602353	1.6297116866190457E-4
36-37	6.518846746476183E-5	6.464523023588882E-4	0.0	0.03722261492237901	1.6297116866190457E-4
38-39	6.518846746476183E-5	6.736141638025389E-4	0.0	0.06182582901803787	1.6297116866190457E-4
40-41	7.062083975349197E-5	7.822616095771419E-4	0.0	0.08908004079059703	1.847006578168252E-4
42-43	7.605321204222213E-5	7.876939818658721E-4	0.0	0.11198835473217209	1.9013303010555535E-4
44-45	7.605321204222213E-5	8.583148216193641E-4	0.0	0.1422738302418427	1.9556540239428548E-4
46-47	7.605321204222213E-5	9.343680336615862E-4	0.0	0.1710110796492252	1.9556540239428548E-4
48-49	7.605321204222213E-5	9.343680336615862E-4	0.0	0.1966464444797428	2.064301469717458E-4
50-51	7.605321204222213E-5	0.0010104212457038085	0.0	0.21663757450226975	2.172948915492061E-4
52-53	7.605321204222213E-5	0.0010267183625699988	0.0	0.25902637547123114	2.390243807041267E-4
54-55	7.605321204222213E-5	0.0010375831071474592	0.0	0.3023278149846992	2.661862421477775E-4
56-57	7.605321204222213E-5	0.0010430154794361893	0.0	0.3451457733644703	2.770509867252378E-4
58-59	7.605321204222213E-5	0.0010430154794361893	0.0	0.3720903399165718	2.8248335901396795E-4
60-61	7.605321204222213E-5	0.0010538802240136495	0.0	0.3911525342777259	2.8248335901396795E-4
62-63	7.605321204222213E-5	0.00106474496859111	0.0	0.4074931101222262	2.933481035914282E-4
64-65	7.605321204222213E-5	0.0011625276697882526	0.0	0.4138598504446179	2.933481035914282E-4
66-67	7.605321204222213E-5	0.0011733924143657128	0.0	0.4197050830272916	2.9878047588015837E-4
68-69	7.605321204222213E-5	0.001205986648098094	0.0	0.4257784752460919	3.042128481688885E-4
70-71	7.605321204222213E-5	0.0012331485095417446	0.0	0.4307219340288363	3.042128481688885E-4
72-73	7.605321204222213E-5	0.0012494456264079351	0.0	0.434231246527356	3.2594233732380915E-4
74-75	7.605321204222213E-5	0.0012494456264079351	0.0	0.43682792048136904	3.2594233732380915E-4
76-77	8.148558433095229E-5	0.001282039860140316	0.0	0.43952780950886794	3.2594233732380915E-4
78-79	8.691795661968244E-5	0.0013037693492952366	0.0	0.44135851897017	3.2594233732380915E-4
80-81	8.691795661968244E-5	0.0013037693492952366	0.0	0.4418637295930219	3.2594233732380915E-4
82-83	8.691795661968244E-5	0.0013037693492952366	0.0	0.4423146164929865	3.3680708190126946E-4
84-85	8.691795661968244E-5	0.0013146340938726968	0.0	0.4424232639387611	3.3680708190126946E-4
86-87	8.691795661968244E-5	0.0013254988384501572	0.0	0.4424938847785146	3.3680708190126946E-4
88	8.691795661968244E-5	0.0014015520504923793	0.0	0.4424993171508033	3.3680708190126946E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12305	0.0	52.139385	1
GTATCAA	22815	0.0	39.395863	1
TCAACGC	27535	0.0	31.993248	4
ATCAACG	28040	0.0	31.416197	3
CAACGCA	28205	0.0	31.23462	5
AACGCAG	28760	0.0	30.631865	6
TATCAAC	29085	0.0	30.527695	2
ACGCAGA	33115	0.0	26.603422	7
CGCAGAG	33735	0.0	26.140387	8
GCAGAGT	39295	0.0	22.208622	9
GTACATG	26190	0.0	21.528633	1
GAGTACT	24515	0.0	21.004044	12-13
TACATGG	26435	0.0	20.618685	2
CAGAGTA	37950	0.0	19.801609	10-11
AGAGTAC	35225	0.0	19.740114	10-11
ACATGGG	27020	0.0	19.509567	3
GTGGTAT	6030	0.0	19.410788	1
GTACTTT	27340	0.0	18.890202	14-15
TGGTATC	5775	0.0	18.830988	2
AGTACTT	26600	0.0	18.405388	12-13
>>END_MODULE