FastQCFastQC Report
Fri 27 May 2016
SRR2139228_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139228_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7958286
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117200.046.488811
CTTATAC74400.041.4121861
GTATCAA211700.035.9306451
TATACAC109900.031.3791893
TTATACA112650.028.754372
TCAACGC259450.028.708984
ATCAACG261950.028.4181393
CAACGCA264150.028.1508675
AACGCAG269000.027.7545246
TATCAAC271200.027.6379472
ACGCAGA307750.024.2459567
ACACATC132850.024.094256
CGCAGAG312800.023.8545178
ACATCTC137400.023.3585748
TACACAT147050.023.1044275
CACATCT148000.021.136967
GTACATG223400.020.9266641
GCAGAGT356500.020.7986819
TACATGG223250.020.3664822
ACATGGG220550.019.9764123
Produced by FastQC (version 0.11.5)