##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139228_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7958286
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.552936649926885	33.0	33.0	33.0	33.0	33.0
2	32.573398719271964	33.0	33.0	33.0	33.0	33.0
3	32.565201351145205	33.0	33.0	33.0	33.0	33.0
4	36.47868825020865	37.0	37.0	37.0	37.0	37.0
5	36.51640240122057	37.0	37.0	37.0	37.0	37.0
6	36.50846941162959	37.0	37.0	37.0	37.0	37.0
7	36.49125552914283	37.0	37.0	37.0	37.0	37.0
8	36.485634972153555	37.0	37.0	37.0	37.0	37.0
9	36.50803552423223	37.0	37.0	37.0	37.0	37.0
10-11	36.46130969658542	37.0	37.0	37.0	37.0	37.0
12-13	36.44391637847647	37.0	37.0	37.0	37.0	37.0
14-15	38.412931503089986	40.0	37.0	40.0	37.0	40.0
16-17	38.38196717232832	40.0	37.0	40.0	37.0	40.0
18-19	38.41416493450977	40.0	37.0	40.0	37.0	40.0
20-21	38.41176220180468	40.0	37.0	40.0	37.0	40.0
22-23	38.41191672640647	40.0	37.0	40.0	37.0	40.0
24-25	38.3865683637313	40.0	37.0	40.0	37.0	40.0
26-27	38.266281367751716	40.0	37.0	40.0	37.0	40.0
28-29	38.208572892704204	40.0	37.0	40.0	37.0	40.0
30-31	38.12792873826446	40.0	37.0	40.0	37.0	40.0
32-33	38.05303801198845	40.0	37.0	40.0	33.0	40.0
34-35	38.03055723941099	40.0	37.0	40.0	33.0	40.0
36-37	37.97327468785717	40.0	37.0	40.0	33.0	40.0
38-39	37.91787918165093	40.0	37.0	40.0	33.0	40.0
40-41	37.90475666290946	40.0	37.0	40.0	33.0	40.0
42-43	37.85162078938485	40.0	37.0	40.0	33.0	40.0
44-45	37.78348677580539	40.0	37.0	40.0	33.0	40.0
46-47	37.74391743782194	40.0	37.0	40.0	33.0	40.0
48-49	37.73652997506567	40.0	37.0	40.0	33.0	40.0
50-51	37.65494806907044	40.0	37.0	40.0	33.0	40.0
52-53	37.564849702865914	40.0	37.0	40.0	33.0	40.0
54-55	37.39240442491802	40.0	37.0	40.0	33.0	40.0
56-57	37.28212104267656	40.0	37.0	40.0	33.0	40.0
58-59	37.1780272139078	37.0	37.0	40.0	33.0	40.0
60-61	37.046056387016094	37.0	37.0	40.0	33.0	40.0
62-63	36.89934549230392	37.0	37.0	40.0	33.0	40.0
64-65	36.72487292749067	37.0	37.0	40.0	33.0	40.0
66-67	36.52874596683951	37.0	37.0	40.0	33.0	40.0
68-69	36.31943507637896	37.0	37.0	40.0	33.0	40.0
70-71	36.06558796834511	37.0	37.0	38.5	33.0	40.0
72-73	35.869993872082496	37.0	37.0	37.0	33.0	40.0
74-75	35.67236072617146	37.0	33.0	37.0	33.0	40.0
76-77	33.86552035946444	35.0	33.0	37.0	27.0	37.0
78-79	35.14206670079028	37.0	33.0	37.0	33.0	37.0
80-81	35.18735445435923	37.0	33.0	37.0	33.0	37.0
82-83	35.04586771540514	37.0	33.0	37.0	33.0	37.0
84-85	34.89808071856556	37.0	33.0	37.0	33.0	37.0
86-87	34.78994617494614	37.0	33.0	37.0	33.0	37.0
88-89	34.665986371513256	37.0	33.0	37.0	33.0	37.0
90-91	34.603013303839994	37.0	33.0	37.0	33.0	37.0
92-93	34.536639423959	37.0	33.0	37.0	33.0	37.0
94-95	34.54790317616847	37.0	33.0	37.0	33.0	37.0
96-97	34.52604094023263	37.0	33.0	37.0	33.0	37.0
98-99	34.51829187236696	37.0	33.0	37.0	33.0	37.0
100	34.49886973274132	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	1.0
16	1.0
17	0.0
18	3.0
19	13.0
20	70.0
21	305.0
22	1245.0
23	3376.0
24	7690.0
25	15079.0
26	26749.0
27	42493.0
28	63072.0
29	88502.0
30	120782.0
31	161253.0
32	215030.0
33	289024.0
34	396450.0
35	567907.0
36	964886.0
37	2225472.0
38	2696256.0
39	72625.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.16429090384538	20.65653332891027	14.412060586915324	23.767115180329032
2	14.190228901047286	21.341127976551736	42.2333150630676	22.23532805933338
3	17.478562595011034	26.98628825352595	31.300332760094324	24.234816391368692
4	11.918827362824176	18.74207970924075	39.471543750036915	29.867549177898166
5	12.772398478767915	38.07907883682491	36.16227162481972	12.986251059587453
6	28.722486035607957	38.77547866202697	19.159046612727856	13.342988689637217
7	25.28068229767063	33.29410378063819	23.71048238276433	17.714731538926852
8	23.803944718749744	37.047638147209085	21.715894603435967	17.43252253060521
9	24.983809327787416	16.891275332401978	21.570172270762825	36.55474306904779
10-11	23.414798607911578	27.047973217798027	29.503844038647287	20.03338413564311
12-13	24.55136045694284	24.703993902201503	29.48655249964754	21.258093141208114
14-15	21.760318676398725	26.08755257335012	27.061872413584688	25.09025633666647
16-17	21.20573450112248	29.347242609778036	27.68747567001236	21.759547219087125
18-19	21.202687362580335	28.149905645512113	29.710461524001524	20.93694546790603
20-21	21.924159666531835	27.314505380142222	29.62724839631161	21.134086557014328
22-23	21.62655025455985	27.503057702850665	29.444542711231264	21.425849331358222
24-25	21.46646916502597	27.741160143441547	29.61353125479804	21.178839436734435
26-27	21.585344286088905	27.814706726848605	29.55022797074445	21.049721016318042
28-29	21.34192157572259	27.60935521121168	29.731864552557763	21.316858660507968
30-31	21.673839300296454	27.632345624720777	29.495355513186205	21.19845956179656
32-33	21.5430974301487	27.559196714751177	29.535067354922763	21.36263850017737
34-35	21.34112920273775	27.87965305933865	29.74363631990744	21.035581418016154
36-37	21.64712304842322	27.735867230639773	29.2926556337289	21.324354087208107
38-39	21.357637379227825	27.699086650443643	29.4926534038454	21.450622566483133
40-41	21.50328393503375	27.586383863853026	29.424647542376682	21.485684658736545
42-43	21.625670268599944	27.616181237178022	29.278077017863723	21.480071476358308
44-45	21.52596627155704	27.868590546248058	29.023770210938405	21.581672971256502
46-47	21.881914939752388	27.71628755373267	28.516748297835708	21.88504920867923
48-49	21.722513921256432	27.84648852503096	28.507620239243053	21.92337731446955
50-51	21.622239052928936	28.063736232190763	28.508345052334118	21.805679662546183
52-53	21.770115501872784	28.090980056666776	28.53478062091482	21.604123820545624
54-55	21.695599073392234	28.04416731980876	28.335845834864514	21.92438777193449
56-57	21.908604737673755	28.231136502476	28.032694795111478	21.827563964738765
58-59	21.620716096844905	28.422587690599826	28.410409898693022	21.546286313862247
60-61	21.93972761164287	28.53605075487686	27.99602474197484	21.528196891505424
62-63	21.733970705749577	28.70062241035935	28.008802396618908	21.556604487272164
64-65	21.76363988896288	28.518777257140588	27.98761799425616	21.729964859640372
66-67	21.830554989893773	28.319688904743284	27.991025922605473	21.85873018275747
68-69	21.846491094350608	28.215512109706857	28.048685470809552	21.889311325132983
70-71	21.83316841965149	28.069900719564522	28.103944835591925	21.992986025192067
72-73	21.93593693098098	28.03760614753717	28.064528640451503	21.961928281030353
74-75	21.809445726528175	28.18071714686213	28.041319037054357	21.968518089555335
76-77	21.917421143487665	28.077791131701808	27.993565954115994	22.01122177069453
78-79	21.899386819877435	28.041457368092242	27.9837460322046	22.075409779825726
80-81	21.932588669846538	28.10874143728514	27.97775959450543	21.98091029836289
82-83	21.93182755848341	28.048479353085114	28.04903916439956	21.970653924031918
84-85	22.01273420384668	28.017377746950906	27.981621257163493	21.988266792038925
86-87	21.939567448730806	28.010913570453027	28.086998561665986	21.962520419150174
88-89	21.984454083864215	27.92166947730772	28.016213892985682	22.07766254584238
90-91	22.00556532651358	27.969297354130155	28.039129907186698	21.986007412169567
92-93	22.001187862484954	27.8705536049877	28.117804921489387	22.01045361103797
94-95	21.91496792263142	27.898050236510358	28.151850008644708	22.035131832213516
96-97	21.988602124532676	28.02880878754322	28.17227737907737	21.810311708846733
98-99	23.079121414902968	29.910932634199185	28.431772185392575	18.57817376550527
100	22.01099046291387	30.911089244007115	22.354838140989493	24.72308215208952
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2395.0
1	2023.0
2	2626.0
3	3829.0
4	5161.5
5	8276.5
6	11111.0
7	13620.0
8	15724.5
9	15703.0
10	13625.5
11	11561.5
12	12277.5
13	16305.5
14	25275.0
15	37334.0
16	47448.5
17	53272.5
18	54191.0
19	51347.5
20	46900.0
21	42833.0
22	41229.0
23	43964.0
24	50927.0
25	62404.0
26	76820.5
27	92122.0
28	110423.0
29	130434.0
30	148620.5
31	166130.0
32	183744.0
33	199977.0
34	216112.0
35	231146.0
36	245791.5
37	261411.0
38	273930.0
39	282815.5
40	291047.0
41	298440.0
42	302959.5
43	307101.5
44	310755.0
45	315003.5
46	325572.5
47	336523.5
48	336314.5
49	329271.5
50	322597.5
51	309872.5
52	289802.5
53	267384.0
54	245938.5
55	224190.0
56	201922.0
57	179482.0
58	156967.5
59	133784.5
60	109807.5
61	88600.5
62	69544.5
63	53030.5
64	39447.5
65	28836.5
66	21234.5
67	15773.0
68	11721.5
69	8406.5
70	5859.0
71	4198.0
72	2975.5
73	2033.5
74	1402.0
75	983.0
76	683.5
77	440.0
78	280.5
79	184.5
80	126.5
81	81.0
82	63.5
83	52.0
84	41.0
85	29.0
86	20.5
87	14.5
88	9.0
89	6.0
90	3.0
91	1.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0010932002192431888
5	0.0
6	1.1308967785274366E-4
7	0.0
8	0.0
9	0.0
10-11	0.00395185596496532
12-13	5.0262079045663855E-5
14-15	0.0014952968516084996
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	141.0
20-21	538.0
22-23	1232.0
24-25	2651.0
26-27	4152.0
28-29	7524.0
30-31	16008.0
32-33	19901.0
34-35	27871.0
36-37	38035.0
38-39	44640.0
40-41	46486.0
42-43	56034.0
44-45	66991.0
46-47	65236.0
48-49	58116.0
50-51	51448.0
52-53	50727.0
54-55	58376.0
56-57	63699.0
58-59	61604.0
60-61	63351.0
62-63	63658.0
64-65	68190.0
66-67	69660.0
68-69	59206.0
70-71	58988.0
72-73	56778.0
74-75	58599.0
76-77	57056.0
78-79	62530.0
80-81	63181.0
82-83	62390.0
84-85	63444.0
86-87	67026.0
88-89	72447.0
90-91	77509.0
92-93	81031.0
94-95	93177.0
96-97	259594.0
98-99	483760.0
100-101	5275301.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.10684256582746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.7641058651768	74.65668677498869
2	7.780521523406159	13.087901976983076
3	2.0020232863394467	5.05151572071817
4	0.6940738006621882	2.335054235254407
5	0.3021357562385096	1.2705842241729774
6	0.16102859940258432	0.8126164235129315
7	0.09355903574763871	0.5508268563166091
8	0.05576847169579413	0.375240805524397
9	0.03101394664644489	0.2347636615143762
>10	0.11331807075009755	1.4435151734280602
>50	0.0021191574443397763	0.12036712946651286
>100	3.080771791582707E-4	0.046740856779311786
>500	2.440931083752482E-5	0.01418616134052664
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.0262079045663855E-5	0.0	0.0	0.0
3	0.0	5.0262079045663855E-5	0.0	0.0	0.0
4	0.0	7.539311856849578E-5	0.0	1.2565519761415964E-5	0.0
5	0.0	7.539311856849578E-5	0.0	1.2565519761415964E-5	0.0
6	0.0	7.539311856849578E-5	0.0	1.2565519761415964E-5	0.0
7	0.0	7.539311856849578E-5	0.0	1.2565519761415964E-5	0.0
8	0.0	8.795863832991174E-5	0.0	1.2565519761415964E-5	0.0
9	0.0	1.7591727665982349E-4	0.0	5.0262079045663855E-5	0.0
10-11	0.0	1.7591727665982349E-4	0.0	6.282759880707982E-5	0.0
12-13	0.0	1.7591727665982349E-4	0.0	8.795863832991174E-5	6.282759880707982E-6
14-15	0.0	2.387448754669033E-4	0.0	8.795863832991174E-5	1.2565519761415964E-5
16-17	0.0	2.513103952283193E-4	0.0	9.424139821061973E-5	1.2565519761415964E-5
18-19	0.0	2.6387591498973524E-4	0.0	1.0052415809132771E-4	1.2565519761415964E-5
20-21	0.0	2.764414347511512E-4	0.0	1.0680691797203569E-4	1.8848279642123944E-5
22-23	0.0	2.8900695451256715E-4	0.0	1.2565519761415964E-4	2.5131039522831927E-5
24-25	0.0	3.32986273677523E-4	0.0	1.5706899701769953E-4	2.5131039522831927E-5
26-27	0.0	3.644000730810629E-4	0.0	2.1989659582477935E-4	2.5131039522831927E-5
28-29	0.0	3.644000730810629E-4	0.0	5.277518299794705E-4	2.5131039522831927E-5
30-31	0.0	4.2722767188814275E-4	0.0	0.0012879657755451362	2.5131039522831927E-5
32-33	0.0	4.2722767188814275E-4	0.0	0.003053421302024079	3.769655928424789E-5
34-35	0.0	4.7120699105309864E-4	0.0	0.0051895596614647925	3.769655928424789E-5
36-37	0.0	5.214690700987625E-4	0.0	0.007457635978400374	3.769655928424789E-5
38-39	0.0	5.340345898601785E-4	0.0	0.011334098824797198	3.769655928424789E-5
40-41	0.0	6.408415078322142E-4	0.0	0.015568678984394378	3.769655928424789E-5
42-43	0.0	6.78538067116462E-4	0.0	0.018911107240931025	3.769655928424789E-5
44-45	0.0	7.853449850884976E-4	0.0	0.023051446002317583	3.769655928424789E-5
46-47	0.0	8.293243042534536E-4	0.0	0.027505922757739543	4.397931916495587E-5
48-49	0.0	8.293243042534536E-4	0.0	0.031790764996382385	5.0262079045663855E-5
50-51	0.0	9.486967419869053E-4	0.0	0.035598117484091425	5.0262079045663855E-5
52-53	0.0	9.549795018676132E-4	0.0	0.03969447692631303	7.539311856849578E-5
54-55	0.0	9.549795018676132E-4	0.0	0.04387251224698384	1.0052415809132771E-4
56-57	0.0	9.549795018676132E-4	0.0	0.04818248552514951	1.0052415809132771E-4
58-59	0.0	9.675450216290291E-4	0.0	0.05236680360570103	1.0052415809132771E-4
60-61	0.0	9.863933012711531E-4	0.0	0.05703489419706706	1.0052415809132771E-4
62-63	0.0	9.926760611518612E-4	0.0	0.06148308819260831	1.0052415809132771E-4
64-65	6.282759880707982E-6	0.0010806346994817729	0.0	0.06522761308151026	1.0052415809132771E-4
66-67	1.2565519761415964E-5	0.0011183312587660207	0.0	0.06969465535669364	1.0680691797203569E-4
68-69	1.2565519761415964E-5	0.0011434622982888526	0.0	0.07413656659235418	1.1308967785274366E-4
70-71	1.2565519761415964E-5	0.0011685933378116845	0.0	0.07787480872137542	1.2565519761415964E-4
72-73	1.2565519761415964E-5	0.0011811588575731005	0.0	0.08114184385934359	1.6335175689840753E-4
74-75	1.2565519761415964E-5	0.0012062898970959324	0.0	0.08385599612780943	1.6335175689840753E-4
76-77	1.2565519761415964E-5	0.0012628347360223043	0.0	0.08645705871842253	1.6335175689840753E-4
78-79	1.2565519761415964E-5	0.0012816830156644283	0.0	0.08815340388621369	1.6335175689840753E-4
80-81	1.2565519761415964E-5	0.0012816830156644283	0.0	0.08878796263416519	1.7591727665982349E-4
82-83	1.2565519761415964E-5	0.0012816830156644283	0.0	0.08927801790486042	1.8848279642123944E-4
84-85	1.2565519761415964E-5	0.0012816830156644283	0.0	0.0894979145006852	1.8848279642123944E-4
86-87	1.2565519761415964E-5	0.0012942485354258443	0.0	0.08959843865877652	1.9476555630194744E-4
88	1.2565519761415964E-5	0.0013445106144715081	0.0	0.08960472141865723	2.0104831618265542E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11720	0.0	46.48881	1
CTTATAC	7440	0.0	41.412186	1
GTATCAA	21170	0.0	35.930645	1
TATACAC	10990	0.0	31.379189	3
TTATACA	11265	0.0	28.75437	2
TCAACGC	25945	0.0	28.70898	4
ATCAACG	26195	0.0	28.418139	3
CAACGCA	26415	0.0	28.150867	5
AACGCAG	26900	0.0	27.754524	6
TATCAAC	27120	0.0	27.637947	2
ACGCAGA	30775	0.0	24.245956	7
ACACATC	13285	0.0	24.09425	6
CGCAGAG	31280	0.0	23.854517	8
ACATCTC	13740	0.0	23.358574	8
TACACAT	14705	0.0	23.104427	5
CACATCT	14800	0.0	21.13696	7
GTACATG	22340	0.0	20.926664	1
GCAGAGT	35650	0.0	20.798681	9
TACATGG	22325	0.0	20.366482	2
ACATGGG	22055	0.0	19.976412	3
>>END_MODULE