FastQCFastQC Report
Fri 27 May 2016
SRR2139227_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139227_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10019720
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140700.051.6336171
GTATCAA245650.036.1926771
ATCAACG292950.029.8205493
TCAACGC300250.029.1393684
CAACGCA308050.028.347675
AACGCAG311400.028.0703356
TATCAAC314000.027.9995082
ACGCAGA316700.027.5735517
CTTATAC59450.023.1523531
CGCAGAG384500.022.7903088
GTGGTAT65500.022.195881
GTACATG307200.022.094431
TGGTATC62650.021.283342
ACATGGG300550.021.1808873
TACATGG311550.021.164772
GCAGAGT428300.020.3090029
GAGTACT251950.019.8552512-13
CAGAGTA414450.019.01007510-11
CATGGGG237850.018.9888344
AGAGTAC383700.018.4594610-11
Produced by FastQC (version 0.11.5)