##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139227_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10019720
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64446551400638	33.0	33.0	33.0	33.0	33.0
2	32.678519160216055	33.0	33.0	33.0	33.0	33.0
3	33.348597964813386	33.0	33.0	33.0	33.0	37.0
4	36.484934309541586	37.0	37.0	37.0	37.0	37.0
5	36.51704468787551	37.0	37.0	37.0	37.0	37.0
6	36.50536292431325	37.0	37.0	37.0	37.0	37.0
7	36.49657635143497	37.0	37.0	37.0	37.0	37.0
8	36.479790952242176	37.0	37.0	37.0	37.0	37.0
9	36.49901863525128	37.0	37.0	37.0	37.0	37.0
10-11	36.47131821048892	37.0	37.0	37.0	37.0	37.0
12-13	36.680884944888675	37.0	37.0	37.0	37.0	38.5
14-15	38.412641920133495	40.0	37.0	40.0	37.0	40.0
16-17	38.38283644652745	40.0	37.0	40.0	37.0	40.0
18-19	38.410226483374785	40.0	37.0	40.0	37.0	40.0
20-21	38.401951380592706	40.0	37.0	40.0	37.0	40.0
22-23	38.40656689002511	40.0	37.0	40.0	37.0	40.0
24-25	38.3623993658194	40.0	37.0	40.0	37.0	40.0
26-27	38.25195113788514	40.0	37.0	40.0	37.0	40.0
28-29	38.20119252568627	40.0	37.0	40.0	37.0	40.0
30-31	38.12150881762989	40.0	37.0	40.0	35.0	40.0
32-33	38.052425976522365	40.0	37.0	40.0	33.0	40.0
34-35	38.0333851447424	40.0	37.0	40.0	33.0	40.0
36-37	37.96810929199425	40.0	37.0	40.0	33.0	40.0
38-39	37.91914058651058	40.0	37.0	40.0	33.0	40.0
40-41	37.88685762874579	40.0	37.0	40.0	33.0	40.0
42-43	37.82234602496196	40.0	37.0	40.0	33.0	40.0
44-45	37.751077181068425	40.0	37.0	40.0	33.0	40.0
46-47	37.71176939500798	40.0	37.0	40.0	33.0	40.0
48-49	37.67925468077388	40.0	37.0	40.0	33.0	40.0
50-51	37.5882718903994	40.0	37.0	40.0	33.0	40.0
52-53	37.47013325065643	40.0	37.0	40.0	33.0	40.0
54-55	37.292638716652235	40.0	37.0	40.0	33.0	40.0
56-57	37.16767446112134	37.0	37.0	40.0	33.0	40.0
58-59	37.043902689250615	37.0	37.0	40.0	33.0	40.0
60-61	36.89374174896448	37.0	37.0	40.0	33.0	40.0
62-63	36.71921601748492	37.0	37.0	40.0	33.0	40.0
64-65	36.52038646276751	37.0	37.0	40.0	33.0	40.0
66-67	36.303306467963054	37.0	37.0	40.0	33.0	40.0
68-69	36.077683554414065	37.0	37.0	40.0	33.0	40.0
70-71	35.82406549247246	37.0	35.0	37.0	33.0	40.0
72-73	35.624000626506145	37.0	33.0	37.0	33.0	40.0
74-75	35.211088803514215	37.0	33.0	37.0	33.0	40.0
76-77	33.829026511361604	35.0	33.0	37.0	27.0	37.0
78-79	34.966751275984436	37.0	33.0	37.0	33.0	37.0
80-81	35.00482779590505	37.0	33.0	37.0	33.0	37.0
82-83	34.87831549238418	37.0	33.0	37.0	33.0	37.0
84-85	34.73943778694713	37.0	33.0	37.0	33.0	37.0
86-87	34.64749861460842	37.0	33.0	37.0	33.0	37.0
88-89	34.53507214806353	37.0	33.0	37.0	33.0	37.0
90-91	34.48282460056156	37.0	33.0	37.0	33.0	37.0
92-93	34.419810759977764	37.0	33.0	37.0	33.0	37.0
94-95	34.437696655021725	37.0	33.0	37.0	33.0	37.0
96-97	34.420568933742786	37.0	33.0	37.0	33.0	37.0
98-99	34.410110020406066	37.0	33.0	37.0	33.0	37.0
100	34.36836398869105	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	0.0
15	0.0
16	3.0
17	1.0
18	1.0
19	12.0
20	84.0
21	453.0
22	1554.0
23	4399.0
24	10161.0
25	19597.0
26	34509.0
27	55147.0
28	81951.0
29	114746.0
30	157431.0
31	210514.0
32	279237.0
33	376699.0
34	516094.0
35	740432.0
36	1268354.0
37	2870291.0
38	3183788.0
39	94260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.81972267083764	4.6907507776166956E-4	24.114411203987977	30.06539705009662
2	15.123083328908724	23.927634276260925	38.07198726989866	22.877295124931685
3	16.809210897295596	24.540821943018837	32.685319725736214	25.964647433949352
4	13.286484644697067	22.96003256805913	36.88344672903688	26.87003605820692
5	17.680737122541775	36.20730643317506	32.05822758684426	14.053728857438902
6	28.41534766667944	36.8341125972483	20.209513007958712	14.541026728113543
7	26.421786237539575	32.559732208085656	22.434079994251334	18.584401560123435
8	24.457606286811835	32.1093385483904	21.816959680948692	21.616095483849076
9	24.02283468189724	20.268489853608965	23.43338418995993	32.27529127453386
10-11	25.02054524495102	25.79711258714388	28.2928383577777	20.8895038101274
12-13	25.274960854612498	23.89380160022771	28.168875132875748	22.662362412284043
14-15	22.679925026872358	26.13480691325693	26.19450496477401	24.990763095096703
16-17	21.976731884723325	28.44492660473546	27.09981416646373	22.478527344077477
18-19	22.07884551664118	27.572257508193843	28.70195973540179	21.646937239763187
20-21	22.78893487331593	26.817970136538015	28.657847916837586	21.735247073308464
22-23	22.757040906879297	26.641528255932673	28.421807453195747	22.179623383992286
24-25	22.416727834290718	26.85435742445832	28.67970681871913	22.049207922531835
26-27	22.310168579183657	27.09512733514432	28.568677701332142	22.026026384339882
28-29	22.351284377242987	26.841534472364927	28.55410408376027	22.253077066631825
30-31	22.500319022372384	26.758846249024735	28.57436492787843	22.166469800724446
32-33	22.265966159975335	27.04482373047029	28.539836903188505	22.14937320636587
34-35	22.549292876454818	26.785813259285668	28.36787860926465	22.29701525499486
36-37	22.424278615135133	26.98323940917885	28.426616879980422	22.165865095705602
38-39	22.330273524909337	27.189962102620658	28.366194266625833	22.113570105844172
40-41	22.59976547765461	26.841743673332317	28.159884982368034	22.39860586664504
42-43	22.435699216975284	26.960709089262952	28.183017943609777	22.420573750151984
44-45	22.446642038512643	27.295451772915403	27.86009180135124	22.397814387220716
46-47	22.716231775666706	27.038510593269276	27.539399478551157	22.70585815251286
48-49	22.539551992838312	27.25339765824375	27.583817396213618	22.62323295270432
50-51	22.604567312577352	27.491053269513436	27.37247963058062	22.531899787328594
52-53	22.91718559087893	27.202565741877777	27.285315676048533	22.59493299119476
54-55	22.753242408192563	27.19039925432762	27.394299390239667	22.66205894724015
56-57	22.75745528176357	27.63200742669169	27.049189723718996	22.56134756782574
58-59	22.771106611654954	27.283302216119765	27.30195791021755	22.643633262007732
60-61	22.769834764480613	27.472006069114137	27.209381271153156	22.548777895252094
62-63	22.67334420878043	27.68312837213824	27.207386213050405	22.436141206030925
64-65	22.932911441130557	27.293048831179334	27.042428025443133	22.731611702246976
66-67	22.76261337935744	27.3645233760603	27.065926908440264	22.806936336141998
68-69	22.769332584189826	27.505062152016635	27.07888590371329	22.64671936008025
70-71	22.861234116889158	27.11365128355813	27.10450012296162	22.92061447659108
72-73	22.783644413434484	27.21257281407828	27.086167551297486	22.917615221189752
74-75	22.693391452700727	27.504467932137505	26.9885658552255	22.813574759936266
76-77	22.939099668980294	27.168654369200095	26.921633072441253	22.97061288937836
78-79	22.678817845329355	27.282954971253044	27.075372270570302	22.962854912847302
80-81	22.741882845879584	27.396248279171793	27.01099358060831	22.850875294340312
82-83	22.980944013943716	27.103852006659825	27.036920207097044	22.878283772299415
84-85	22.871842428880484	27.168910741113912	27.145262014793037	22.81398481521257
86-87	22.759772882629715	27.28907667359787	27.113688816532722	22.8374616272397
88-89	22.927105948568528	27.077730546477113	27.022341896209184	22.97282160874517
90-91	22.90975274985611	27.145951349965635	27.092192373719087	22.852103526459167
92-93	22.90613313699781	27.21609052441229	27.136158787365545	22.74161755122436
94-95	22.830048148964146	27.052917517935043	27.188239265697252	22.928795067403556
96-97	22.863260337954262	27.377162536346823	27.236869720058465	22.522707405640453
98-99	24.07305910081587	29.463544832113676	26.725984882306086	19.73741118476437
100	23.09268641907686	29.82793933201498	21.15409101232627	25.92528323658189
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1897.0
1	1742.5
2	2655.5
3	3890.5
4	5227.0
5	8669.0
6	11566.5
7	13541.0
8	15162.5
9	15277.5
10	14034.0
11	12482.0
12	13138.0
13	16631.5
14	25515.0
15	39522.5
16	52268.0
17	59925.0
18	61190.5
19	57059.0
20	50426.5
21	43503.5
22	38775.5
23	38792.5
24	43903.5
25	54311.5
26	68288.0
27	84189.0
28	102231.0
29	123126.0
30	143965.0
31	164400.5
32	186583.5
33	208559.5
34	230982.5
35	252252.5
36	274592.0
37	296041.5
38	312955.5
39	327168.5
40	340167.0
41	351436.5
42	359107.0
43	367920.5
44	377574.5
45	388508.5
46	398238.0
47	406457.5
48	416001.5
49	417285.5
50	413589.0
51	410825.5
52	398108.5
53	377348.5
54	356586.0
55	335080.0
56	311630.0
57	285167.5
58	256691.5
59	224168.0
60	188724.0
61	156919.0
62	127388.0
63	100575.0
64	78128.0
65	58010.0
66	43290.0
67	32338.5
68	22916.0
69	16010.0
70	11419.5
71	8321.0
72	5871.5
73	4065.0
74	2807.5
75	2023.0
76	1592.5
77	1049.5
78	628.5
79	475.0
80	325.0
81	213.0
82	150.5
83	99.0
84	65.5
85	44.5
86	33.0
87	25.5
88	19.5
89	15.5
90	10.0
91	6.5
92	4.5
93	2.5
94	1.5
95	1.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.9960637622608215E-5
2	3.992127524521643E-5
3	2.295473326599945E-4
4	8.682877365834573E-4
5	1.9960637622608215E-5
6	7.984255049043286E-5
7	0.0
8	1.497047821695616E-4
9	4.890356217539014E-4
10-11	0.00369271796018252
12-13	2.0958669503738628E-4
14-15	0.001297441445469534
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	226.0
20-21	791.0
22-23	1468.0
24-25	3670.0
26-27	4895.0
28-29	9123.0
30-31	18550.0
32-33	23265.0
34-35	33335.0
36-37	45350.0
38-39	51256.0
40-41	53999.0
42-43	65713.0
44-45	78005.0
46-47	75552.0
48-49	66984.0
50-51	58796.0
52-53	58186.0
54-55	63129.0
56-57	66016.0
58-59	66016.0
60-61	66033.0
62-63	64591.0
64-65	70635.0
66-67	75512.0
68-69	72935.0
70-71	71233.0
72-73	69873.0
74-75	72485.0
76-77	71532.0
78-79	77557.0
80-81	78782.0
82-83	78446.0
84-85	81472.0
86-87	86221.0
88-89	93374.0
90-91	98364.0
92-93	104624.0
94-95	141930.0
96-97	490449.0
98-99	1798058.0
100-101	5411289.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.9294417600242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.82410355526245	73.66133322672496
2	7.61549323214438	12.630972049379515
3	1.9225942137356127	4.783189946284411
4	0.7192176229701073	2.3857726390753022
5	0.3375561776008046	1.3996672685541156
6	0.19058836089549802	0.9483231825013009
7	0.09744192266538976	0.5656562975464957
8	0.06471740464631671	0.4293582591581313
9	0.049492544962866675	0.3693950212548089
>10	0.17189932146943235	2.2947182292751873
>50	0.005179300239441262	0.2876496636532429
>100	0.0016472100405158564	0.20660306486376231
>500	6.913336717984536E-5	0.037361151728726796
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.988191286782465E-5	0.0	9.980318811304109E-6	0.0
3	0.0	5.988191286782465E-5	0.0	9.980318811304109E-6	0.0
4	0.0	5.988191286782465E-5	0.0	9.980318811304109E-6	0.0
5	0.0	5.988191286782465E-5	0.0	1.9960637622608218E-5	0.0
6	4.9901594056520544E-5	5.988191286782465E-5	0.0	1.9960637622608218E-5	0.0
7	4.9901594056520544E-5	5.988191286782465E-5	0.0	1.9960637622608218E-5	0.0
8	4.9901594056520544E-5	8.982286930173698E-5	0.0	1.9960637622608218E-5	0.0
9	6.986223167912875E-5	1.7964573860347396E-4	0.0	1.9960637622608218E-5	0.0
10-11	7.48523910847808E-5	1.7964573860347396E-4	0.0	2.4950797028260272E-5	0.0
12-13	8.483270989608493E-5	1.8463589800912601E-4	0.0	2.9940956433912326E-5	0.0
14-15	9.481302870738903E-5	2.7944892671651506E-4	0.0	2.9940956433912326E-5	4.9901594056520545E-6
16-17	1.197638257356493E-4	3.393308395843397E-4	0.0	2.9940956433912326E-5	9.980318811304109E-6
18-19	1.2475398514130134E-4	3.493111583956438E-4	0.0	5.48917534621726E-5	9.980318811304109E-6
20-21	1.297441445469534E-4	3.493111583956438E-4	0.0	7.485239108478082E-5	9.980318811304109E-6
22-23	1.297441445469534E-4	4.391340276973808E-4	0.0	1.1477366632999724E-4	9.980318811304109E-6
24-25	1.297441445469534E-4	4.890356217539013E-4	0.0	1.497047821695616E-4	9.980318811304109E-6
26-27	1.397244633582575E-4	5.239667375934656E-4	0.0	2.3952765147129858E-4	1.9960637622608218E-5
28-29	1.397244633582575E-4	5.289568969991177E-4	0.0	7.285632732251998E-4	1.9960637622608218E-5
30-31	1.397244633582575E-4	5.688781722443341E-4	0.0	0.0024351977899582026	1.9960637622608218E-5
32-33	1.397244633582575E-4	5.688781722443341E-4	0.0	0.005084972434359443	1.9960637622608218E-5
34-35	1.397244633582575E-4	6.587010415460711E-4	0.0	0.008443349714363276	1.9960637622608218E-5
36-37	1.6966541979216985E-4	7.535140702534602E-4	0.0	0.012205929906224924	1.9960637622608218E-5
38-39	1.6966541979216985E-4	7.684845484704164E-4	0.0	0.01693660102278307	2.994095643391233E-5
40-41	1.6966541979216985E-4	8.28366461338241E-4	0.0	0.02242577636900033	4.9901594056520544E-5
42-43	1.8962605741477807E-4	0.0010080121999417148	0.0	0.02774528629542542	5.988191286782465E-5
44-45	1.8962605741477807E-4	0.0011776776197338847	0.0	0.03287517016443573	5.988191286782465E-5
46-47	1.9461621682043012E-4	0.0011776776197338847	0.0	0.03763578223742779	6.986223167912875E-5
48-49	2.2954733265999447E-4	0.0011826677791395368	0.0	0.042251679687655946	6.986223167912875E-5
50-51	2.594882890939068E-4	0.0013423528801204026	0.0	0.0460442008359515	6.986223167912875E-5
52-53	2.594882890939068E-4	0.0013473430395260547	0.0	0.04975687943375663	6.986223167912875E-5
54-55	2.594882890939068E-4	0.0013473430395260547	0.0	0.05324001069890177	6.986223167912875E-5
56-57	2.9441940493347117E-4	0.0013573233583373588	0.0	0.05723712838282906	6.986223167912875E-5
58-59	2.994095643391232E-4	0.0013573233583373588	0.0	0.061199314950916794	6.986223167912875E-5
60-61	3.0938988315042733E-4	0.001382274155365619	0.0	0.0647173773319015	6.986223167912875E-5
62-63	3.592914772069479E-4	0.0014022347929882272	0.0	0.06822545939407489	6.986223167912875E-5
64-65	3.592914772069479E-4	0.0014870675028843122	0.0	0.07187326591960655	6.986223167912875E-5
66-67	3.592914772069479E-4	0.0015269887781295286	0.0	0.07576559025601515	6.986223167912875E-5
68-69	3.592914772069479E-4	0.0015469494157521369	0.0	0.0799223930409233	6.986223167912875E-5
70-71	3.942225930465123E-4	0.001556929734563441	0.0	0.08387459929019972	6.986223167912875E-5
72-73	4.1917339007477257E-4	0.0015719002127803972	0.0	0.08694853748408139	7.984255049043287E-5
74-75	4.1917339007477257E-4	0.0016367722850538738	0.0	0.0895184695779922	7.984255049043287E-5
76-77	4.1917339007477257E-4	0.001731585313761263	0.0	0.09184887402043171	7.984255049043287E-5
78-79	4.1917339007477257E-4	0.0017665164296008272	0.0	0.0935255675807308	7.984255049043287E-5
80-81	4.1917339007477257E-4	0.0017764967484121313	0.0	0.09409943591238079	8.483270989608493E-5
82-83	4.1917339007477257E-4	0.0017864770672234354	0.0	0.09447369786780468	8.982286930173698E-5
84-85	4.1917339007477257E-4	0.0017864770672234354	0.0	0.09464835344700251	8.982286930173698E-5
86-87	4.241635494804246E-4	0.0018164180236573478	0.0	0.09474815663511556	8.982286930173698E-5
88	4.2915370888607667E-4	0.001926201530581693	0.0	0.09476312711333251	8.982286930173698E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14070	0.0	51.633617	1
GTATCAA	24565	0.0	36.192677	1
ATCAACG	29295	0.0	29.820549	3
TCAACGC	30025	0.0	29.139368	4
CAACGCA	30805	0.0	28.34767	5
AACGCAG	31140	0.0	28.070335	6
TATCAAC	31400	0.0	27.999508	2
ACGCAGA	31670	0.0	27.573551	7
CTTATAC	5945	0.0	23.152353	1
CGCAGAG	38450	0.0	22.790308	8
GTGGTAT	6550	0.0	22.19588	1
GTACATG	30720	0.0	22.09443	1
TGGTATC	6265	0.0	21.28334	2
ACATGGG	30055	0.0	21.180887	3
TACATGG	31155	0.0	21.16477	2
GCAGAGT	42830	0.0	20.309002	9
GAGTACT	25195	0.0	19.85525	12-13
CAGAGTA	41445	0.0	19.010075	10-11
CATGGGG	23785	0.0	18.988834	4
AGAGTAC	38370	0.0	18.45946	10-11
>>END_MODULE