FastQCFastQC Report
Fri 27 May 2016
SRR2139226_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139226_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9108104
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117850.047.9882551
GTATCAA219250.038.013781
TCAACGC267100.030.6641274
ATCAACG268800.030.4535623
TATCAAC277400.029.7696322
CAACGCA275250.029.710395
AACGCAG281000.029.1326586
CTTATAC125150.027.1066741
ACGCAGA321000.025.5288737
CGCAGAG325050.025.1717478
TATACAC156500.023.6300223
GCAGAGT382650.021.3610579
TTATACA164150.021.2096392
GTACATG256500.020.9559171
TACATGG261600.020.271462
GAGTACT235100.019.3511612-13
ACATGGG265750.019.2998523
TACACAT199200.018.8000355
AGAGTAC344850.018.65392310-11
CAGAGTA368850.018.49348310-11
Produced by FastQC (version 0.11.5)