##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139226_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9108104
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.522394562029596	33.0	33.0	33.0	33.0	33.0
2	32.542124464103615	33.0	33.0	33.0	33.0	33.0
3	32.53702581788701	33.0	33.0	33.0	33.0	33.0
4	36.44157367987893	37.0	37.0	37.0	37.0	37.0
5	36.475762683430055	37.0	37.0	37.0	37.0	37.0
6	36.477965886204196	37.0	37.0	37.0	37.0	37.0
7	36.457375102436245	37.0	37.0	37.0	37.0	37.0
8	36.450813583156275	37.0	37.0	37.0	37.0	37.0
9	36.475900802186715	37.0	37.0	37.0	37.0	37.0
10-11	36.426688144975074	37.0	37.0	37.0	37.0	37.0
12-13	36.407024228094016	37.0	37.0	37.0	37.0	37.0
14-15	38.34938286826764	40.0	37.0	40.0	37.0	40.0
16-17	38.31388019943558	40.0	37.0	40.0	37.0	40.0
18-19	38.34457961832672	40.0	37.0	40.0	37.0	40.0
20-21	38.34113431307652	40.0	37.0	40.0	37.0	40.0
22-23	38.34174869252506	40.0	37.0	40.0	37.0	40.0
24-25	38.31515287081983	40.0	37.0	40.0	37.0	40.0
26-27	38.18392025381115	40.0	37.0	40.0	37.0	40.0
28-29	38.124306018019354	40.0	37.0	40.0	33.0	40.0
30-31	38.03649609439785	40.0	37.0	40.0	33.0	40.0
32-33	37.960116320763305	40.0	37.0	40.0	33.0	40.0
34-35	37.94052344428263	40.0	37.0	40.0	33.0	40.0
36-37	37.87432151466045	40.0	37.0	40.0	33.0	40.0
38-39	37.821148191381525	40.0	37.0	40.0	33.0	40.0
40-41	37.79426557781537	40.0	37.0	40.0	33.0	40.0
42-43	37.73755240411935	40.0	37.0	40.0	33.0	40.0
44-45	37.66625193240536	40.0	37.0	40.0	33.0	40.0
46-47	37.62155268456481	40.0	37.0	40.0	33.0	40.0
48-49	37.61329076481559	40.0	37.0	40.0	33.0	40.0
50-51	37.51448558680665	40.0	37.0	40.0	33.0	40.0
52-53	37.41192856273518	40.0	37.0	40.0	33.0	40.0
54-55	37.23664121237803	40.0	37.0	40.0	33.0	40.0
56-57	37.119870556000194	38.5	37.0	40.0	33.0	40.0
58-59	37.00917736090646	37.0	37.0	40.0	33.0	40.0
60-61	36.87273682889583	37.0	37.0	40.0	33.0	40.0
62-63	36.715847274342515	37.0	37.0	40.0	33.0	40.0
64-65	36.53600238558212	37.0	37.0	40.0	33.0	40.0
66-67	36.333410689748845	37.0	37.0	40.0	33.0	40.0
68-69	36.120208806691366	37.0	37.0	40.0	33.0	40.0
70-71	35.86691625631474	37.0	35.0	37.0	33.0	40.0
72-73	35.67500594648649	37.0	33.0	37.0	33.0	40.0
74-75	35.4790981785533	37.0	33.0	37.0	33.0	40.0
76-77	33.660835401534094	35.0	33.0	37.0	27.0	37.0
78-79	34.96089479363998	37.0	33.0	37.0	33.0	37.0
80-81	35.021614950605255	37.0	33.0	37.0	33.0	37.0
82-83	34.8907632932665	37.0	33.0	37.0	33.0	37.0
84-85	34.754963685030916	37.0	33.0	37.0	33.0	37.0
86-87	34.659060311668775	37.0	33.0	37.0	33.0	37.0
88-89	34.545380631046285	37.0	33.0	37.0	33.0	37.0
90-91	34.48945791774858	37.0	33.0	37.0	33.0	37.0
92-93	34.43427672185756	37.0	33.0	37.0	33.0	37.0
94-95	34.45542644304322	37.0	33.0	37.0	33.0	37.0
96-97	34.44292785877805	37.0	33.0	37.0	33.0	37.0
98-99	34.44439984607048	37.0	33.0	37.0	33.0	37.0
100	34.432079166267634	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	8.0
19	17.0
20	107.0
21	483.0
22	1738.0
23	4953.0
24	10778.0
25	21292.0
26	35670.0
27	56187.0
28	81445.0
29	112435.0
30	150976.0
31	198280.0
32	261258.0
33	345042.0
34	466379.0
35	663056.0
36	1118464.0
37	2561211.0
38	2936832.0
39	81491.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.08636660275289	20.360285741137783	14.354282735462837	24.199064920646492
2	14.523670348955173	21.016909776172955	41.76889064946997	22.690529225401907
3	17.968602466550667	26.325017808316638	30.64523637411255	25.06114335102015
4	12.448833319535906	18.338658701195808	38.29158348867334	30.92092449059495
5	13.412110797153831	37.50237151442276	35.47766911752435	13.607848570899058
6	29.765989433792562	37.68945925343538	18.761303820679036	13.78324749209302
7	26.027524499061492	32.642435791247	23.088757001457164	18.241282708234337
8	24.196671447756852	36.537110248192164	21.433198391234885	17.833019912816102
9	25.29310161587966	16.797842888047832	21.112198543187475	36.79685695288504
10-11	24.055608999142432	26.648229589916134	28.83606784980392	20.460093561137516
12-13	25.011267439553546	24.244104398028878	28.84508442647168	21.89954373594589
14-15	22.197330144922287	25.660296981165427	26.416523523040496	25.725849350871787
16-17	21.812838325078413	28.791612392656035	27.1249482878105	22.270600994455048
18-19	21.906299049725387	27.641878046188317	28.945749850902008	21.506073053184284
20-21	22.459063326642195	26.955519255848063	28.899964234022036	21.685453183487702
22-23	22.244828221188314	27.176950014016732	28.68309261485677	21.89512914993818
24-25	22.31896680490634	27.190454433627387	28.78966519347696	21.700913567989318
26-27	22.168049162825078	27.47017623564228	28.797520873407596	21.564253728125053
28-29	22.089288610668305	27.3010419934135	28.822504406290093	21.787164989628106
30-31	22.124521495119943	27.318607289816377	28.887799527463187	21.669071687600493
32-33	22.145929835005447	27.4403150511828	28.720916557463898	21.69283855634786
34-35	22.371821836561814	27.26268594469535	28.796983666339443	21.568508552403394
36-37	21.99316401589259	27.49336489286182	28.574077986934416	21.93939310431118
38-39	21.919632194388548	27.234767441113362	28.783409414766588	22.0621909497315
40-41	22.137914445804363	27.07054614226669	28.78881746140648	22.00272195052247
42-43	22.167577848518143	27.147685940693624	28.60506942274133	22.079666788046904
44-45	22.066233534592573	27.390504591296605	28.367065910014993	22.17619596409583
46-47	22.394600093588735	27.224140411203052	27.90963815389024	22.471621341317974
48-49	22.21484676158995	27.37034021534099	27.95956187324075	22.45525114982831
50-51	22.142073835409875	27.61550572650946	27.896479212037544	22.345941226043124
52-53	22.28893631680253	27.607871214856033	27.90865662157765	22.19453584676378
54-55	22.205098971622796	27.547935332805178	27.729335387648163	22.51763030792386
56-57	22.40720408867751	27.71492765227064	27.486150132325864	22.39171812672599
58-59	22.15278765848853	27.891532683221033	27.78031308501324	22.175366573277195
60-61	22.40416615767545	28.009004537659226	27.43139662836897	22.155432676296353
62-63	22.242516442845485	28.12473497534628	27.40757556786334	22.225173013944893
64-65	22.25593696839333	28.018117146075554	27.38356797341266	22.342377912118458
66-67	22.36886098579827	27.85406153810946	27.29335075117918	22.48372672491309
68-69	22.33452440986816	27.776896385236583	27.408670560226163	22.4799086446691
70-71	22.362773648409163	27.5999449583836	27.42040536418401	22.616876029023224
72-73	22.450373316075314	27.560581330006073	27.369297601563947	22.619747752354666
74-75	22.34498917621761	27.697107488080242	27.344655397431804	22.61324793827034
76-77	22.51776132344418	27.59544191789211	27.274661431942587	22.612135326721123
78-79	22.46488638105987	27.612407297771774	27.24916468872346	22.6735416324449
80-81	22.447184119173606	27.629523777425767	27.263637895588282	22.65965420781234
82-83	22.50116365662716	27.595107593010255	27.349552366115436	22.554176384247153
84-85	22.61584644290168	27.531464410692518	27.297381787931062	22.555307358474735
86-87	22.473518291935925	27.530669833939765	27.408712444671096	22.587099429453215
88-89	22.50712399447125	27.506174410736023	27.381966600614394	22.604734994178333
90-91	22.576711906568118	27.494155288415723	27.38385626352688	22.545276541489283
92-93	22.632788747670478	27.417037052342646	27.46529322184244	22.484880978144435
94-95	22.514159881386153	27.436061930748924	27.491912424541475	22.55786576332345
96-97	22.593305049139513	27.52829765306597	27.54241295669186	22.33598434110266
98-99	23.645149305016012	29.47913212750624	27.732220412761066	19.143498154716678
100	22.544686221662815	30.4520268698781	21.607164844786777	25.396122063672312
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1852.0
1	1734.0
2	2670.0
3	3965.0
4	5484.0
5	8922.5
6	11822.0
7	13919.5
8	15291.5
9	15028.5
10	13620.5
11	12262.0
12	13289.0
13	17294.5
14	26541.5
15	39278.5
16	49674.0
17	55892.0
18	57149.0
19	53757.0
20	48404.5
21	43433.5
22	40857.5
23	42424.0
24	48421.5
25	59153.5
26	73710.0
27	89656.5
28	106506.0
29	125573.0
30	144686.0
31	163282.5
32	183928.5
33	203991.0
34	222734.0
35	241244.5
36	261446.0
37	280780.0
38	295868.5
39	308393.0
40	322024.0
41	335036.5
42	343962.5
43	352388.0
44	358688.5
45	365217.0
46	377375.5
47	389958.0
48	392341.0
49	388578.5
50	386953.5
51	378367.0
52	358861.0
53	336474.0
54	315591.5
55	293535.5
56	270178.0
57	244117.5
58	216419.0
59	188262.0
60	157607.5
61	128017.5
62	101420.5
63	78459.0
64	58615.5
65	42789.5
66	31786.0
67	24082.0
68	17939.0
69	12638.0
70	8779.0
71	6332.5
72	4497.5
73	3079.0
74	2069.5
75	1357.0
76	888.0
77	589.5
78	381.0
79	242.0
80	170.0
81	130.5
82	99.5
83	65.5
84	39.0
85	26.0
86	21.0
87	18.5
88	9.5
89	3.0
90	3.0
91	3.0
92	2.0
93	2.0
94	3.0
95	3.0
96	3.0
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0013833834132767919
5	0.0
6	1.2077156782575167E-4
7	0.0
8	0.0
9	0.0
10-11	0.004056826755601385
12-13	1.0979233438704698E-5
14-15	0.0013065287792058589
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	163.0
20-21	674.0
22-23	1411.0
24-25	3296.0
26-27	3870.0
28-29	8308.0
30-31	20489.0
32-33	24939.0
34-35	34573.0
36-37	48229.0
38-39	53230.0
40-41	58383.0
42-43	68140.0
44-45	80076.0
46-47	76911.0
48-49	70447.0
50-51	60941.0
52-53	60678.0
54-55	70640.0
56-57	75561.0
58-59	74030.0
60-61	75894.0
62-63	78226.0
64-65	82626.0
66-67	87764.0
68-69	75819.0
70-71	74264.0
72-73	72822.0
74-75	75809.0
76-77	73423.0
78-79	78657.0
80-81	80155.0
82-83	79306.0
84-85	82033.0
86-87	86249.0
88-89	91922.0
90-91	96852.0
92-93	101548.0
94-95	116420.0
96-97	305793.0
98-99	550093.0
100-101	5847440.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.29412426634204
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.10250078876743	75.10817273930202
2	7.59459196911994	12.803589583943193
3	1.8665730271675347	4.720234161127872
4	0.6674180668362326	2.250376858539807
5	0.30191968030363414	1.27250275249844
6	0.1485278698662406	0.7512016031711979
7	0.09080487227951156	0.5358022031542926
8	0.059311776272781745	0.3999707391676274
9	0.03997193911171541	0.3032459642384637
>10	0.12551687674305767	1.6355388605498005
>50	0.0022963255136383208	0.12788975244681963
>100	5.450732345240598E-4	0.07859501649184988
>500	2.1734783742491955E-5	0.012879765368692566
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0979233438704696E-5
2	0.0	1.0979233438704696E-5	0.0	5.489616719352348E-5	1.0979233438704696E-5
3	0.0	1.0979233438704696E-5	0.0	5.489616719352348E-5	1.0979233438704696E-5
4	0.0	3.2937700316114086E-5	0.0	6.587540063222817E-5	2.1958466877409392E-5
5	0.0	6.587540063222817E-5	0.0	7.685463407093288E-5	2.1958466877409392E-5
6	0.0	6.587540063222817E-5	0.0	7.685463407093288E-5	2.1958466877409392E-5
7	0.0	7.685463407093288E-5	0.0	7.685463407093288E-5	2.1958466877409392E-5
8	0.0	8.783386750963757E-5	0.0	8.783386750963757E-5	2.1958466877409392E-5
9	0.0	1.9762620189668455E-4	0.0	1.0979233438704696E-4	4.3916933754818784E-5
10-11	0.0	1.9762620189668455E-4	0.0	1.0979233438704696E-4	4.9406550474171137E-5
12-13	0.0	2.031158186160369E-4	0.0	1.4273003470316106E-4	5.489616719352348E-5
14-15	0.0	2.6899121924826505E-4	0.0	1.6468850158057045E-4	6.587540063222817E-5
16-17	0.0	2.8546006940632213E-4	0.0	2.250742854934463E-4	7.685463407093288E-5
18-19	0.0	3.2388738644178853E-4	0.0	2.909496861256745E-4	1.26261184545104E-4
20-21	0.0	3.678043201966073E-4	0.0	3.403562365998456E-4	1.3175080126445635E-4
22-23	0.0	4.062316372320738E-4	0.0	6.367955394448723E-4	1.4273003470316106E-4
24-25	0.0	4.501485709868926E-4	0.0	0.001405341880154201	1.5370926814186576E-4
26-27	0.0	4.6112780442559724E-4	0.0	0.003409051982717808	1.5370926814186576E-4
28-29	0.0	4.6112780442559724E-4	0.0	0.012126563333049338	1.5370926814186576E-4
30-31	0.0	5.105343548997684E-4	0.0	0.017753420470385493	1.5370926814186576E-4
32-33	0.0	5.379824384965301E-4	0.0	0.02943532484916729	1.7566773501927514E-4
34-35	0.0	6.203266892868154E-4	0.0	0.04325817974849651	1.9762620189668455E-4
36-37	0.0	7.2462940695451E-4	0.0	0.06188993889397837	2.0860543533538924E-4
38-39	0.0	7.520774905512717E-4	0.0	0.09788535572277172	2.415431356515033E-4
40-41	0.0	8.728490583770234E-4	0.0	0.13615347387337695	2.415431356515033E-4
42-43	0.0	9.057867586931374E-4	0.0	0.16695571328566297	2.415431356515033E-4
44-45	0.0	0.0010155790930801844	0.0	0.2017214559693214	2.415431356515033E-4
46-47	2.1958466877409392E-5	0.0010979233438704696	0.0	0.2365585636703314	2.5252236909020803E-4
48-49	2.1958466877409392E-5	0.0010979233438704696	0.0	0.26251896113614864	2.635016025289127E-4
50-51	2.1958466877409392E-5	0.001279080695609097	0.0	0.28387905979114864	2.635016025289127E-4
52-53	2.1958466877409392E-5	0.0012845703123284494	0.0	0.329706380164302	2.6899121924826505E-4
54-55	2.1958466877409392E-5	0.0013120183959252113	0.0	0.3803975009508016	2.744808359676174E-4
56-57	2.1958466877409392E-5	0.0013504457129606777	0.0	0.43062200431615627	2.7997045268696977E-4
58-59	2.744808359676174E-5	0.0013833834132767917	0.0	0.46531089236574374	2.9643930284502685E-4
60-61	3.2937700316114086E-5	0.0014108314968735536	0.0	0.49095289206183856	3.6231470347725494E-4
62-63	3.2937700316114086E-5	0.0014547484306283724	0.0	0.5101775298130105	4.0623163723207376E-4
64-65	3.2937700316114086E-5	0.001624926548928295	0.0	0.5158318350339435	4.172108706707785E-4
66-67	3.2937700316114086E-5	0.001641395399086352	0.0	0.5216233806728601	4.172108706707785E-4
68-69	3.2937700316114086E-5	0.0016743330994024664	0.0	0.5271349558590899	4.227004873901308E-4
70-71	3.842731703546643E-5	0.0016798227161218186	0.0	0.5323226436588778	4.501485709868926E-4
72-73	4.9406550474171137E-5	0.0016798227161218186	0.0	0.5367692332015532	5.050447381804161E-4
74-75	5.489616719352348E-5	0.0017182500331572848	0.0	0.5400794720833227	5.050447381804161E-4
76-77	5.489616719352348E-5	0.0017951046672282178	0.0	0.5433567732647762	5.050447381804161E-4
78-79	5.489616719352348E-5	0.0018115735173862748	0.0	0.5457118188373782	5.050447381804161E-4
80-81	5.489616719352348E-5	0.0018115735173862748	0.0	0.5464364482443327	5.105343548997684E-4
82-83	5.489616719352348E-5	0.0018225527508249795	0.0	0.5469140448989164	5.215135883384731E-4
84-85	5.489616719352348E-5	0.001828042367544332	0.0	0.5471446088011291	5.324928217771777E-4
86-87	5.489616719352348E-5	0.0018664696845797984	0.0	0.5472269530519195	5.379824384965301E-4
88	5.489616719352348E-5	0.0019213658517733218	0.0	0.5472379322853582	5.379824384965301E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11785	0.0	47.988255	1
GTATCAA	21925	0.0	38.01378	1
TCAACGC	26710	0.0	30.664127	4
ATCAACG	26880	0.0	30.453562	3
TATCAAC	27740	0.0	29.769632	2
CAACGCA	27525	0.0	29.71039	5
AACGCAG	28100	0.0	29.132658	6
CTTATAC	12515	0.0	27.106674	1
ACGCAGA	32100	0.0	25.528873	7
CGCAGAG	32505	0.0	25.171747	8
TATACAC	15650	0.0	23.630022	3
GCAGAGT	38265	0.0	21.361057	9
TTATACA	16415	0.0	21.209639	2
GTACATG	25650	0.0	20.955917	1
TACATGG	26160	0.0	20.27146	2
GAGTACT	23510	0.0	19.35116	12-13
ACATGGG	26575	0.0	19.299852	3
TACACAT	19920	0.0	18.800035	5
AGAGTAC	34485	0.0	18.653923	10-11
CAGAGTA	36885	0.0	18.493483	10-11
>>END_MODULE