FastQCFastQC Report
Fri 27 May 2016
SRR2139225_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139225_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9273015
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136700.050.2011151
CTTATAC144800.036.131221
GTATCAA244600.036.094291
TATACAC183100.030.8927363
TTATACA184050.029.2796782
TCAACGC301850.028.728754
ATCAACG304350.028.5059533
CAACGCA308850.028.0640245
TATCAAC311250.028.0377372
AACGCAG313150.027.732916
ACGCAGA359150.024.1807447
TACACAT235950.023.595945
CGCAGAG372400.023.3320478
GTACATG315450.021.8353821
ACACATC246350.021.7033966
TACATGG316750.021.3435632
ACATCTC251450.021.2970928
ATACACA281550.021.0713864
ACATGGG317650.020.9087663
GCAGAGT424000.020.47319
Produced by FastQC (version 0.11.5)