##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139225_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9273015
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51887611526564	33.0	33.0	33.0	33.0	33.0
2	32.541961702855005	33.0	33.0	33.0	33.0	33.0
3	32.53493691102624	33.0	33.0	33.0	33.0	33.0
4	36.44083623287572	37.0	37.0	37.0	37.0	37.0
5	36.47573081678397	37.0	37.0	37.0	37.0	37.0
6	36.47477298375987	37.0	37.0	37.0	37.0	37.0
7	36.45258063315976	37.0	37.0	37.0	37.0	37.0
8	36.44713256691594	37.0	37.0	37.0	37.0	37.0
9	36.472156790429004	37.0	37.0	37.0	37.0	37.0
10-11	36.42165476924172	37.0	37.0	37.0	37.0	37.0
12-13	36.39967001023939	37.0	37.0	37.0	37.0	37.0
14-15	38.33756000610373	40.0	37.0	40.0	37.0	40.0
16-17	38.2996252567261	40.0	37.0	40.0	37.0	40.0
18-19	38.33088709551316	40.0	37.0	40.0	37.0	40.0
20-21	38.33142315177056	40.0	37.0	40.0	37.0	40.0
22-23	38.328286601261794	40.0	37.0	40.0	37.0	40.0
24-25	38.299646714791535	40.0	37.0	40.0	37.0	40.0
26-27	38.162351866576955	40.0	37.0	40.0	35.0	40.0
28-29	38.09159053269675	40.0	37.0	40.0	33.0	40.0
30-31	38.013875958111235	40.0	37.0	40.0	33.0	40.0
32-33	37.93063933079446	40.0	37.0	40.0	33.0	40.0
34-35	37.9009187831314	40.0	37.0	40.0	33.0	40.0
36-37	37.83050173672693	40.0	37.0	40.0	33.0	40.0
38-39	37.762583350888505	40.0	37.0	40.0	33.0	40.0
40-41	37.74285070856894	40.0	37.0	40.0	33.0	40.0
42-43	37.68838733895072	40.0	37.0	40.0	33.0	40.0
44-45	37.61352499906394	40.0	37.0	40.0	33.0	40.0
46-47	37.56524085848765	40.0	37.0	40.0	33.0	40.0
48-49	37.55491367205164	40.0	37.0	40.0	33.0	40.0
50-51	37.445342871857235	40.0	37.0	40.0	33.0	40.0
52-53	37.33770498840311	40.0	37.0	40.0	33.0	40.0
54-55	37.159348130729676	40.0	37.0	40.0	33.0	40.0
56-57	37.03721281579445	37.0	37.0	40.0	33.0	40.0
58-59	36.920077468454934	37.0	37.0	40.0	33.0	40.0
60-61	36.77848637738383	37.0	37.0	40.0	33.0	40.0
62-63	36.62650233612706	37.0	37.0	40.0	33.0	40.0
64-65	36.44521281218635	37.0	37.0	40.0	33.0	40.0
66-67	36.24375923343387	37.0	37.0	40.0	33.0	40.0
68-69	36.03361873931199	37.0	37.0	40.0	33.0	40.0
70-71	35.78148567933424	37.0	35.0	37.0	33.0	40.0
72-73	35.59223811565571	37.0	33.0	37.0	33.0	40.0
74-75	35.40495103703991	37.0	33.0	37.0	33.0	40.0
76-77	33.57241167788321	35.0	33.0	37.0	27.0	37.0
78-79	34.89250954710574	37.0	33.0	37.0	33.0	37.0
80-81	34.961345110627896	37.0	33.0	37.0	33.0	37.0
82-83	34.834939508130994	37.0	33.0	37.0	33.0	37.0
84-85	34.69899665009744	37.0	33.0	37.0	33.0	37.0
86-87	34.60979827077888	37.0	33.0	37.0	33.0	37.0
88-89	34.49754315245359	37.0	33.0	37.0	33.0	37.0
90-91	34.444898571542154	37.0	33.0	37.0	33.0	37.0
92-93	34.387119291920015	37.0	33.0	37.0	30.0	37.0
94-95	34.409803603938265	37.0	33.0	37.0	33.0	37.0
96-97	34.40061356332017	37.0	33.0	37.0	33.0	37.0
98-99	34.404738283546145	37.0	33.0	37.0	33.0	37.0
100	34.387664526643405	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	2.0
19	26.0
20	111.0
21	524.0
22	1952.0
23	5290.0
24	11792.0
25	22685.0
26	38170.0
27	59318.0
28	86054.0
29	119208.0
30	159121.0
31	207846.0
32	272272.0
33	358296.0
34	483240.0
35	686691.0
36	1165106.0
37	2634556.0
38	2882834.0
39	77920.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.06930701611072	20.346230433143912	14.33955407168003	24.24490847906533
2	14.523863058562938	20.917997005289003	41.78969838828041	22.768441547867656
3	17.835364226198276	26.535533480750328	30.79978841832996	24.829313874721436
4	12.350235632865662	18.502399465108002	37.90824876791511	31.239116134111228
5	13.334681330721454	37.63445869547283	35.36859370981283	13.662266263992887
6	29.593344292744835	37.5557154941376	18.767834026600223	14.083106186517336
7	26.052896495907753	32.78766399062225	22.77100813489464	18.388431378575362
8	24.509363998656315	36.311490922855185	21.293807893117826	17.88533718537067
9	25.414528068810416	16.620365652379512	20.857024387429547	37.10808189138053
10-11	24.2132694148715	26.518679078368883	28.568002211234827	20.700049295524785
12-13	25.2422796537501	24.122115210982926	28.512031175194558	22.123573960072417
14-15	22.415428387358794	25.427599038529664	26.114133245104732	26.04283932900681
16-17	21.96322339605835	28.754590605105246	26.72989313615906	22.55229286267735
18-19	21.9508379960563	27.619069957290048	28.607356938385198	21.822735108268454
20-21	22.787526277380213	26.69124524451269	28.53715672518139	21.98407175292571
22-23	22.550612725992305	27.108528561891976	28.308818235848076	22.032040476267643
24-25	22.381826239279416	27.32523267537467	28.412982096019086	21.879958989326827
26-27	22.22124497234164	27.669502388018003	28.42451785173833	21.684734787902034
28-29	22.121759439092344	27.329212833780574	28.525730537469023	22.023297189658063
30-31	22.701312485547547	27.002476389865297	28.45523520190302	21.840975922684137
32-33	22.282339110318937	27.23330967640574	28.553772557602812	21.930578655672516
34-35	22.286708209573103	26.969942597202618	28.72045272868525	22.022896464539038
36-37	22.174146093510576	27.317686959123094	28.41299934200958	22.095167605356746
38-39	22.132471719734337	26.883580482528043	28.67819504287355	22.305752754864073
40-41	22.24419651246203	26.709736300057457	28.76619111203162	22.279876075448897
42-43	22.360974466502885	26.763234718736108	28.59512866715484	22.28066214760617
44-45	22.1003554595501	27.12112363108436	28.334714668299338	22.443806241066206
46-47	22.61928302947685	26.810053840813435	27.763245735041004	22.80741739466871
48-49	22.46550292767972	27.016601440892252	27.767617783819887	22.750277847608142
50-51	22.288665741701188	27.27380613337815	27.799306310356208	22.63822181456445
52-53	22.416851734891353	27.29809568020951	27.93898181931323	22.34607076558591
54-55	22.3445139583026	27.358883505870224	27.53109907220735	22.765503463619833
56-57	22.625699998342817	27.5665744011251	27.209598311694556	22.59812728883753
58-59	22.196246121697552	27.83812982513843	27.650887218487448	22.314736834676573
60-61	22.593953574961272	28.040124111958008	27.1093802348168	22.256542078263923
62-63	22.351894319690633	28.229346413911642	27.121050763223288	22.29770850317444
64-65	22.422283628143095	27.937181893754282	27.121267210499738	22.519267267602885
66-67	22.51987836426279	27.738773834092267	27.04207094923248	22.69927685241246
68-69	22.53752117397295	27.58777548668726	27.169753221088094	22.704950118251695
70-71	22.53946572662068	27.37856379133483	27.22287035765633	22.859100124388156
72-73	22.630801634156313	27.387383325382388	27.11528716665339	22.866527873807907
74-75	22.51146527485167	27.550215448704286	27.123013613334813	22.815305663109235
76-77	22.689427056371407	27.42801851668967	27.038923279750033	22.84363114718889
78-79	22.594992706664435	27.40175452791171	27.045902645680815	22.95735011974304
80-81	22.600276083089213	27.469545755200986	27.04441804972475	22.885760111985054
82-83	22.737250035579805	27.37300218657707	27.1489247071165	22.740823070726627
84-85	22.78216992296872	27.33673481049256	27.099993785455112	22.781101481083606
86-87	22.62119660588238	27.40646183149317	27.170253989926174	22.80208757269827
88-89	22.66951345684063	27.324466545141128	27.159172622543633	22.84684737547461
90-91	22.77217124160299	27.316767354382765	27.13565144314386	22.775409960870384
92-93	22.846310859983255	27.18701945538123	27.233518443319348	22.733151241316175
94-95	22.58789588067484	27.298383516322843	27.298289743982036	22.81543085902028
96-97	22.7109369589611	27.3634271997002	27.311719289486508	22.613916551852192
98-99	23.865270325436956	29.2386265743937	27.513618370171166	19.38248472999818
100	22.716261462794858	30.30503456638478	21.33632005125066	25.642383919569706
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2005.0
1	1865.0
2	2831.5
3	4192.0
4	5817.5
5	9640.0
6	12848.5
7	14933.5
8	16277.0
9	15870.5
10	14224.0
11	12649.5
12	13640.5
13	18031.5
14	28325.0
15	42751.0
16	54538.5
17	61280.0
18	62378.5
19	58213.5
20	51334.5
21	44549.5
22	40326.0
23	40526.5
24	45288.0
25	54949.5
26	68727.0
27	84246.5
28	100785.0
29	119633.0
30	138161.5
31	157149.0
32	177688.0
33	196943.0
34	216985.0
35	235533.0
36	254145.0
37	273359.0
38	289137.0
39	301550.0
40	313312.0
41	325027.0
42	333844.0
43	342695.5
44	352521.5
45	364179.5
46	383772.5
47	406093.0
48	411207.0
49	407441.0
50	408446.0
51	401088.0
52	379545.0
53	352125.0
54	328838.0
55	307526.5
56	285468.0
57	259943.0
58	232062.0
59	201275.5
60	167378.0
61	137963.5
62	110383.0
63	85741.0
64	65747.5
65	48459.0
66	36212.0
67	27507.0
68	20024.5
69	14140.0
70	10117.0
71	7382.5
72	5251.0
73	3603.5
74	2481.0
75	1707.0
76	1105.5
77	689.0
78	473.0
79	342.5
80	230.5
81	147.5
82	115.5
83	88.0
84	53.5
85	29.0
86	19.5
87	16.0
88	13.5
89	12.0
90	10.0
91	6.0
92	2.5
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.00124015759707064
5	0.0
6	1.1862377015458295E-4
7	0.0
8	0.0
9	0.0
10-11	0.003968504310626048
12-13	1.617596865744313E-5
14-15	0.0014450532000649197
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	186.0
20-21	700.0
22-23	1488.0
24-25	3243.0
26-27	4185.0
28-29	8537.0
30-31	19966.0
32-33	24348.0
34-35	33766.0
36-37	47162.0
38-39	52911.0
40-41	57428.0
42-43	68524.0
44-45	80822.0
46-47	78221.0
48-49	71063.0
50-51	60469.0
52-53	59293.0
54-55	71373.0
56-57	79260.0
58-59	78674.0
60-61	85819.0
62-63	86024.0
64-65	88632.0
66-67	93370.0
68-69	75301.0
70-71	73250.0
72-73	71096.0
74-75	74611.0
76-77	72041.0
78-79	78535.0
80-81	80331.0
82-83	78717.0
84-85	80344.0
86-87	84905.0
88-89	91699.0
90-91	96336.0
92-93	101892.0
94-95	116607.0
96-97	311838.0
98-99	560954.0
100-101	5969094.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.7072773821949
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.63822449344191	71.60680717968727
2	8.186105603105274	13.37728802385725
3	2.2711331330032407	5.567043146105673
4	0.8822928753469147	2.883589947932187
5	0.39598735032275567	1.6177524136330894
6	0.20788216676699495	1.0191291517705532
7	0.12155005047063988	0.6952066582737054
8	0.08014389611300278	0.5238671640155944
9	0.0532961547011155	0.39192153260115736
>10	0.15917650063486818	2.007677243669195
>50	0.0032561718120921266	0.18183724281759464
>100	9.275953470778192E-4	0.11750287810324192
>500	2.4008934094646072E-5	0.01037741753354982
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.0783979104962086E-5	0.0	1.0783979104962086E-5	0.0
3	0.0	1.0783979104962086E-5	0.0	2.1567958209924173E-5	0.0
4	0.0	9.705581194465878E-5	0.0	5.391989552481043E-5	0.0
5	0.0	1.0783979104962086E-4	0.0	6.470387462977252E-5	0.0
6	6.470387462977252E-5	1.0783979104962086E-4	0.0	7.54878537347346E-5	0.0
7	6.470387462977252E-5	1.0783979104962086E-4	0.0	1.0783979104962086E-4	0.0
8	6.470387462977252E-5	1.1862377015458295E-4	0.0	1.509757074694692E-4	0.0
9	6.470387462977252E-5	2.372475403091659E-4	0.0	2.1567958209924173E-4	0.0
10-11	9.705581194465878E-5	2.372475403091659E-4	0.0	2.5881549851909007E-4	0.0
12-13	1.509757074694692E-4	2.372475403091659E-4	0.0	2.9655942538645735E-4	0.0
14-15	1.6715167612691234E-4	3.343033522538247E-4	0.0	3.127353940439005E-4	0.0
16-17	2.0489560299427964E-4	3.396953418063057E-4	0.0	3.235193731488626E-4	0.0
18-19	2.0489560299427964E-4	3.77439268673673E-4	0.0	3.936152373311162E-4	0.0
20-21	2.1567958209924173E-4	4.2057518509352137E-4	0.0	4.906710492757749E-4	0.0
22-23	2.2107157165172277E-4	4.906710492757749E-4	0.0	6.901746627175735E-4	0.0
24-25	2.48031519414128E-4	5.230229865906611E-4	0.0	0.0015475010015620592	0.0
26-27	2.5881549851909007E-4	5.391989552481043E-4	0.0	0.0034508733135878676	0.0
28-29	2.5881549851909007E-4	5.391989552481043E-4	0.0	0.011954040837850472	0.0
30-31	2.5881549851909007E-4	6.308627776402821E-4	0.0	0.016305376406702675	0.0
32-33	2.5881549851909007E-4	6.36254767192763E-4	0.0	0.024991871575749634	0.0
34-35	2.9116743583397633E-4	7.764464955572702E-4	0.0	0.035980746283706	0.0
36-37	3.235193731488626E-4	9.004622552643342E-4	0.0	0.051256252685884796	0.0
38-39	3.235193731488626E-4	9.274222030267394E-4	0.0	0.08304742308731303	0.0
40-41	3.235193731488626E-4	0.0010676139313912465	0.0	0.11864533811279288	0.0
42-43	3.343033522538247E-4	0.0010999658687061327	0.0	0.14521706262741946	0.0
44-45	3.343033522538247E-4	0.001234765607518159	0.0	0.17669549763480377	0.0
46-47	3.343033522538247E-4	0.0013372134090152987	0.0	0.2064700639436041	0.0
48-49	3.343033522538247E-4	0.0013533893776727418	0.0	0.23228151793133084	0.0
50-51	3.4508733135878676E-4	0.0015313250329046161	0.0	0.25283039011583613	0.0
52-53	3.5587131046374885E-4	0.0015421090120095783	0.0	0.29918532429851563	0.0
54-55	3.5587131046374885E-4	0.0015744609493244647	0.0	0.3521238777247745	0.0
56-57	3.5587131046374885E-4	0.0016175968657443128	0.0	0.40424824072860877	0.0
58-59	3.5587131046374885E-4	0.001644556813506718	0.0	0.43713398500919065	0.0
60-61	3.6126330001622986E-4	0.0016876927299265664	0.0	0.4576990331623534	0.0
62-63	3.990072268835972E-4	0.0016930847194790476	0.0	0.4713084147928155	0.0
64-65	3.990072268835972E-4	0.0019626841971030996	0.0	0.47646854879453987	0.0
66-67	3.990072268835972E-4	0.002000428123970467	0.0	0.4813536913290877	0.0
68-69	3.990072268835972E-4	0.0020705239881527206	0.0	0.4860177622919838	0.0
70-71	4.1518319554104035E-4	0.00211905189412505	0.0	0.49029900199665377	0.0
72-73	4.583191119608887E-4	0.002124443883677531	0.0	0.4937930112266614	0.0
74-75	4.637111015133697E-4	0.0022107157165172277	0.0	0.49666154966858134	0.0
76-77	4.637111015133697E-4	0.0023131635180143675	0.0	0.4998266475358877	0.0
78-79	4.744950806183318E-4	0.0023401234657767726	0.0	0.5018917795344879	0.0
80-81	4.744950806183318E-4	0.0023401234657767726	0.0	0.5024201945106311	0.0
82-83	4.744950806183318E-4	0.0023724754030916588	0.0	0.5027976337793048	0.0
84-85	4.744950806183318E-4	0.0023724754030916588	0.0	0.5030510572882714	0.0
86-87	4.852790597232939E-4	0.0023886513717491023	0.0	0.5031642890688734	0.0
88	5.06847017933218E-4	0.002545019068771052	0.0	0.503169681058426	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13670	0.0	50.201115	1
CTTATAC	14480	0.0	36.13122	1
GTATCAA	24460	0.0	36.09429	1
TATACAC	18310	0.0	30.892736	3
TTATACA	18405	0.0	29.279678	2
TCAACGC	30185	0.0	28.72875	4
ATCAACG	30435	0.0	28.505953	3
CAACGCA	30885	0.0	28.064024	5
TATCAAC	31125	0.0	28.037737	2
AACGCAG	31315	0.0	27.73291	6
ACGCAGA	35915	0.0	24.180744	7
TACACAT	23595	0.0	23.59594	5
CGCAGAG	37240	0.0	23.332047	8
GTACATG	31545	0.0	21.835382	1
ACACATC	24635	0.0	21.703396	6
TACATGG	31675	0.0	21.343563	2
ACATCTC	25145	0.0	21.297092	8
ATACACA	28155	0.0	21.071386	4
ACATGGG	31765	0.0	20.908766	3
GCAGAGT	42400	0.0	20.4731	9
>>END_MODULE