FastQCFastQC Report
Fri 27 May 2016
SRR2139224_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139224_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9147816
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121450.053.0709461
GTATCAA222200.039.0161631
TCAACGC275050.031.06084
ATCAACG279000.030.6051183
CAACGCA282750.030.1851435
TATCAAC287200.029.9889052
AACGCAG288800.029.5528036
ACGCAGA330650.025.7861527
GTACATG299500.023.9910661
CGCAGAG354850.023.9910588
TACATGG297800.023.572682
ACATGGG297550.023.0659313
GCAGAGT390000.021.772999
CATGGGG222950.020.9067254
GAGTACT237400.020.67879712-13
GTGGTAT57850.020.1618651
TGGTATC53150.019.815792
CAGAGTA383300.019.33175710-11
AGAGTAC355800.019.2968510-11
GTACTTT272250.017.99993514-15
Produced by FastQC (version 0.11.5)