##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139224_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9147816
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51740743364318	33.0	33.0	33.0	33.0	33.0
2	32.538649553073654	33.0	33.0	33.0	33.0	33.0
3	32.533244219166626	33.0	33.0	33.0	33.0	33.0
4	36.436946698534385	37.0	37.0	37.0	37.0	37.0
5	36.474767201264214	37.0	37.0	37.0	37.0	37.0
6	36.47636419447002	37.0	37.0	37.0	37.0	37.0
7	36.45334853696227	37.0	37.0	37.0	37.0	37.0
8	36.44718826876273	37.0	37.0	37.0	37.0	37.0
9	36.47189110493696	37.0	37.0	37.0	37.0	37.0
10-11	36.42134095176378	37.0	37.0	37.0	37.0	37.0
12-13	36.40078096236304	37.0	37.0	37.0	37.0	37.0
14-15	38.34393963542773	40.0	37.0	40.0	37.0	40.0
16-17	38.31066081783892	40.0	37.0	40.0	37.0	40.0
18-19	38.33656721997907	40.0	37.0	40.0	37.0	40.0
20-21	38.33224541317777	40.0	37.0	40.0	37.0	40.0
22-23	38.33128587785495	40.0	37.0	40.0	37.0	40.0
24-25	38.30670371621275	40.0	37.0	40.0	37.0	40.0
26-27	38.179760638231905	40.0	37.0	40.0	37.0	40.0
28-29	38.112774952342185	40.0	37.0	40.0	33.0	40.0
30-31	38.03586005767376	40.0	37.0	40.0	33.0	40.0
32-33	37.957890949395065	40.0	37.0	40.0	33.0	40.0
34-35	37.9314546551956	40.0	37.0	40.0	33.0	40.0
36-37	37.8677633427389	40.0	37.0	40.0	33.0	40.0
38-39	37.80730007580091	40.0	37.0	40.0	33.0	40.0
40-41	37.77303146936156	40.0	37.0	40.0	33.0	40.0
42-43	37.709961662893974	40.0	37.0	40.0	33.0	40.0
44-45	37.629605458455984	40.0	37.0	40.0	33.0	40.0
46-47	37.57658830931837	40.0	37.0	40.0	33.0	40.0
48-49	37.5458353589581	40.0	37.0	40.0	33.0	40.0
50-51	37.452441595542226	40.0	37.0	40.0	33.0	40.0
52-53	37.342676560705975	40.0	37.0	40.0	33.0	40.0
54-55	37.156092068095234	40.0	37.0	40.0	33.0	40.0
56-57	37.03965122516804	37.0	37.0	40.0	33.0	40.0
58-59	36.90857775133674	37.0	37.0	40.0	33.0	40.0
60-61	36.76853998242701	37.0	37.0	40.0	33.0	40.0
62-63	36.60409093993346	37.0	37.0	40.0	33.0	40.0
64-65	36.41220318316235	37.0	37.0	40.0	33.0	40.0
66-67	36.205274611120466	37.0	37.0	40.0	33.0	40.0
68-69	35.9886919747327	37.0	37.0	40.0	33.0	40.0
70-71	35.731251116127254	37.0	33.0	37.0	33.0	40.0
72-73	35.542821236624036	37.0	33.0	37.0	33.0	40.0
74-75	35.35604805349487	37.0	33.0	37.0	33.0	40.0
76-77	33.52553814118855	35.0	33.0	37.0	27.0	37.0
78-79	34.85378915426232	37.0	33.0	37.0	33.0	37.0
80-81	34.9177592675086	37.0	33.0	37.0	33.0	37.0
82-83	34.787734500998624	37.0	33.0	37.0	33.0	37.0
84-85	34.649380197175496	37.0	33.0	37.0	33.0	37.0
86-87	34.55529173769339	37.0	33.0	37.0	33.0	37.0
88-89	34.43574098980572	37.0	33.0	37.0	30.0	37.0
90-91	34.376789505485185	37.0	33.0	37.0	27.0	37.0
92-93	34.3088366034238	37.0	33.0	37.0	27.0	37.0
94-95	34.32508758005454	37.0	33.0	37.0	27.0	37.0
96-97	34.31979795758933	37.0	33.0	37.0	30.0	37.0
98-99	34.3306864219623	37.0	33.0	37.0	33.0	37.0
100	34.31219896202009	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	3.0
18	5.0
19	19.0
20	84.0
21	488.0
22	1776.0
23	4911.0
24	11340.0
25	22295.0
26	37972.0
27	59052.0
28	86119.0
29	118908.0
30	158930.0
31	207065.0
32	270616.0
33	357253.0
34	484975.0
35	694501.0
36	1194262.0
37	2638616.0
38	2741359.0
39	57266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.65934251410391	20.30104234715696	14.175514680225312	23.86410045851381
2	14.86421458411494	20.987621526274687	41.59745889073414	22.550704998876235
3	18.348117189939106	26.152854408090416	30.148988567325794	25.350039834644683
4	12.535951604958354	17.913174810668544	38.28650524054425	31.264368343828853
5	13.60755397791123	37.54211934302133	35.31192581923379	13.538400859833647
6	30.35007519835904	37.50744167508581	18.481819575098115	13.66066355145704
7	26.59600936442097	32.43464888231246	23.025146111377843	17.94419564188873
8	24.561184877352147	36.72596825296879	20.916631904270922	17.796214965408137
9	25.717132920032498	16.477167883569148	21.10868867497991	36.69701052141844
10-11	24.55941253984992	26.645000145395777	28.426273300766137	20.369314013988166
12-13	25.198194934482693	24.276460862991062	28.63625203491283	21.889092167613413
14-15	22.318671676533558	25.464933382300202	26.382187661430933	25.834207279735306
16-17	21.905392500242684	28.652986680099378	26.99250837576969	22.449112443888247
18-19	21.955634000508976	27.593165406912423	28.798994208016428	21.652206384562174
20-21	22.806669353362462	26.769263245372926	28.782814752397734	21.641252648866878
22-23	22.56007608380917	26.82023150176038	28.570240015708304	22.049452398722146
24-25	22.332732622004077	26.913710089287672	28.808424808268	21.94513248044025
26-27	22.270635505838417	27.02330280321225	28.76827024456803	21.937791446381304
28-29	22.20836193408472	26.97215232953975	28.742612935464397	22.076872800911136
30-31	22.584679905294568	26.795660672573735	28.687134987660702	21.93252443447099
32-33	22.472426067235062	26.8236119668652	28.673505458394555	22.030456507505182
34-35	22.346283435208758	26.990517609392263	28.731030079113552	21.932168876285427
36-37	22.544422024419806	26.937020115947824	28.45913164932867	22.059426210303705
38-39	22.20958108252953	27.12826882672264	28.569366129786523	22.092783960961306
40-41	22.400763298112082	26.943032853986004	28.54179960823618	22.114404239665735
42-43	22.336681956323197	27.044401660734714	28.31237030222273	22.306546080719354
44-45	22.264673670902805	27.209092588904333	28.12120522076202	22.405028519430843
46-47	22.524897172588624	27.126620520835242	27.836603411422622	22.51187889515351
48-49	22.491298034620943	27.38140936506996	27.683396627261825	22.443895973047272
50-51	22.429883180104543	27.461734082518113	27.67029517210633	22.438087565271015
52-53	22.684225527657325	27.393888514118213	27.58426663909768	22.337619319126777
54-55	22.65354503033288	27.340470999292933	27.48500155189319	22.520982418481005
56-57	22.71681496639909	27.583004288293868	27.267091912033592	22.433088833273455
58-59	22.427134369412045	27.569031838283202	27.557919991068346	22.445913801236408
60-61	22.695277357235387	27.600519119946217	27.387564503480384	22.316639019338012
62-63	22.51965053461373	27.636376681869624	27.48768163272809	22.356291150788557
64-65	22.584327981475248	27.48749019046296	27.416502044790608	22.511679783271184
66-67	22.66139838991225	27.529858950344092	27.337572665453997	22.471169994289657
68-69	22.599885067570554	27.57062471663311	27.327134598220688	22.50235561757565
70-71	22.62570728458749	27.38372999969388	27.252512337057144	22.738050378661484
72-73	22.633575605983268	27.476707820267627	27.10013747415434	22.789579099594768
74-75	22.55846242528342	27.49251981391855	27.245894782333746	22.703122978464283
76-77	22.663462723690735	27.34543827544642	27.187951847594892	22.803147153267954
78-79	22.53745870249665	27.360205821207	27.120340611369663	22.981994864926683
80-81	22.735150479801305	27.439497436872905	27.150271668536746	22.67508041478904
82-83	22.84473638990281	27.27408490744232	27.25476046309701	22.62641823955786
84-85	22.707639389380617	27.24479620436555	27.191341208919606	22.85622319733422
86-87	22.59817531404685	27.4530913133401	27.203836750140315	22.744896622472737
88-89	22.852553761287155	27.317128142758452	27.122062366581357	22.70825572937304
90-91	22.850637738387043	27.337190896701753	27.18748551579766	22.62468584911354
92-93	22.785652984023137	27.18671039118868	27.281057623155775	22.746579001632412
94-95	22.674162688033555	27.430539143463566	27.310853523256135	22.584444645246744
96-97	22.844874415980456	27.350913207498785	27.413082720715188	22.391129655805575
98-99	23.823978237415098	29.368872466887474	27.60671315948922	19.200436136208204
100	22.674686939546397	30.478724586157092	21.543876077261256	25.302712397035247
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2280.0
1	2013.5
2	2892.5
3	4271.0
4	5640.5
5	9071.5
6	12150.5
7	14605.5
8	16487.0
9	16262.5
10	14296.5
11	12354.0
12	12976.0
13	16774.0
14	26211.0
15	39279.5
16	49765.0
17	55584.0
18	56296.0
19	52528.5
20	46636.5
21	40934.5
22	37780.5
23	39040.0
24	44876.5
25	55511.0
26	69149.5
27	82878.0
28	97580.5
29	115227.0
30	132012.5
31	148611.5
32	167380.5
33	185987.5
34	204634.0
35	222525.5
36	241993.0
37	260127.0
38	274394.0
39	287785.0
40	301374.5
41	313387.0
42	320594.0
43	327233.0
44	334763.0
45	345115.0
46	355375.5
47	367520.0
48	375427.0
49	374701.0
50	375362.0
51	373291.5
52	353648.0
53	328498.0
54	312249.0
55	293647.0
56	272114.5
57	247134.5
58	220887.5
59	193076.5
60	162815.0
61	137638.0
62	112140.0
63	89674.5
64	73193.0
65	56318.0
66	44417.0
67	33018.0
68	21823.0
69	15902.5
70	11390.5
71	7273.0
72	4980.0
73	3660.5
74	2831.0
75	2126.0
76	1339.0
77	701.5
78	490.5
79	394.0
80	284.0
81	193.0
82	144.5
83	97.5
84	59.5
85	44.5
86	35.0
87	25.0
88	18.5
89	11.0
90	6.5
91	5.0
92	3.5
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0011150202408968437
5	0.0
6	1.093157098918474E-4
7	0.0
8	0.0
9	0.0
10-11	0.004044681265998354
12-13	4.372628395673896E-5
14-15	0.0014702962980453476
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	173.0
20-21	619.0
22-23	1292.0
24-25	2763.0
26-27	3555.0
28-29	6619.0
30-31	14013.0
32-33	17505.0
34-35	23991.0
36-37	32590.0
38-39	37749.0
40-41	42040.0
42-43	52490.0
44-45	63048.0
46-47	61461.0
48-49	54726.0
50-51	45956.0
52-53	43796.0
54-55	49708.0
56-57	54025.0
58-59	35974.0
60-61	57581.0
62-63	57928.0
64-65	61614.0
66-67	65315.0
68-69	62778.0
70-71	61386.0
72-73	60022.0
74-75	63328.0
76-77	60151.0
78-79	66464.0
80-81	68910.0
82-83	68390.0
84-85	70729.0
86-87	76126.0
88-89	83349.0
90-91	88752.0
92-93	95580.0
94-95	112733.0
96-97	335434.0
98-99	586453.0
100-101	6300700.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.37598953281257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.25469273906327	72.70067645294665
2	7.9703176969741705	13.1312561435827
3	2.050541534458636	5.06746163937486
4	0.7505803850617733	2.4731920777353222
5	0.359981320535496	1.482690874622003
6	0.19333420320898634	0.9555657779926868
7	0.11944637675921767	0.688765943715479
8	0.06898907756178609	0.4546434824886468
9	0.05398512310477913	0.40023701422261954
>10	0.1732126757017878	2.2672968880296867
>50	0.0038639809688535355	0.21609200940751558
>100	0.0010054939894737543	0.1298563877438385
>500	4.9392611757620816E-5	0.03226530813784349
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.186314197836948E-5	0.0	0.0	0.0
3	0.0	2.186314197836948E-5	0.0	0.0	0.0
4	0.0	7.652099692429319E-5	0.0	1.093157098918474E-5	0.0
5	0.0	7.652099692429319E-5	0.0	1.093157098918474E-5	0.0
6	0.0	7.652099692429319E-5	0.0	2.186314197836948E-5	1.093157098918474E-5
7	0.0	7.652099692429319E-5	0.0	2.186314197836948E-5	1.093157098918474E-5
8	0.0	9.838413890266267E-5	0.0	2.186314197836948E-5	2.186314197836948E-5
9	0.0	1.9676827780532534E-4	0.0	4.372628395673896E-5	6.558942593510844E-5
10-11	0.0	1.9676827780532534E-4	0.0	6.012364044051607E-5	7.652099692429319E-5
12-13	0.0	1.9676827780532534E-4	0.0	8.745256791347792E-5	7.652099692429319E-5
14-15	0.0	2.623577037404338E-4	0.0	9.838413890266267E-5	9.29183534080703E-5
16-17	5.46578549459237E-6	2.732892747296185E-4	0.0	1.0384992439725505E-4	1.1478149538643978E-4
18-19	1.093157098918474E-5	3.0061820220258036E-4	0.0	1.311788518702169E-4	1.2571306637562451E-4
20-21	1.093157098918474E-5	3.3887870066472695E-4	0.0	1.5850777934317876E-4	1.3664463736480926E-4
22-23	1.093157098918474E-5	3.6074184264309645E-4	0.0	2.5689191824584143E-4	1.5850777934317876E-4
24-25	1.639735648377711E-5	4.153996975890202E-4	0.0	5.90304833415976E-4	1.9130249231073297E-4
26-27	2.732892747296185E-5	4.263312685782049E-4	0.0	0.00147576208353994	2.022340632999177E-4
28-29	3.279471296755422E-5	4.263312685782049E-4	0.0	0.005760937911300359	2.0769984879451007E-4
30-31	6.012364044051607E-5	4.973864800079057E-4	0.0	0.007488126127591548	2.1316563428910244E-4
32-33	6.558942593510844E-5	5.192496219862753E-4	0.0	0.011314175973806207	2.404945617620643E-4
34-35	7.105521142970082E-5	6.34031117372715E-4	0.0	0.015484570306180185	2.404945617620643E-4
36-37	7.652099692429319E-5	7.542783982537471E-4	0.0	0.022234815392001762	2.623577037404338E-4
38-39	7.652099692429319E-5	7.761415402321166E-4	0.0	0.038626706090284284	2.732892747296185E-4
40-41	7.652099692429319E-5	9.401151050698877E-4	0.0	0.056488893086612146	2.732892747296185E-4
42-43	7.652099692429319E-5	9.729098180374419E-4	0.0	0.0681364819755885	2.787550602242109E-4
44-45	8.198678241888555E-5	0.0011259518118860283	0.0	0.08316739208571751	2.8422084571880327E-4
46-47	8.745256791347792E-5	0.0012352675217778756	0.0	0.0985863729659626	3.006182022025804E-4
48-49	8.745256791347792E-5	0.0012352675217778756	0.0	0.1126826337565163	3.0608398769717276E-4
50-51	9.838413890266267E-5	0.0013500490171643154	0.0	0.12589343729694608	3.0608398769717276E-4
52-53	9.838413890266267E-5	0.0013555148026589078	0.0	0.15755126688162507	3.115497731917651E-4
54-55	1.0384992439725505E-4	0.001371912159142685	0.0	0.19570244963387984	3.279471296755422E-4
56-57	1.093157098918474E-4	0.0013992410866156468	0.0	0.23530206554220157	3.3887870066472695E-4
58-59	1.093157098918474E-4	0.0014047068721102392	0.0	0.3684868606889339	3.552760571485041E-4
60-61	1.2024728088103215E-4	0.001415638443099424	0.0	0.4832410271479006	3.6074184264309645E-4
62-63	1.2024728088103215E-4	0.0014648305125507554	0.0	0.48959226989261695	3.8260498462146594E-4
64-65	1.2024728088103215E-4	0.0016834619323344501	0.0	0.4945005452667609	3.8260498462146594E-4
66-67	1.2024728088103215E-4	0.0017053250743128195	0.0	0.49887863944792943	3.880707701160583E-4
68-69	1.2024728088103215E-4	0.0017326540017857813	0.0	0.503202075774152	3.935365556106507E-4
70-71	1.2024728088103215E-4	0.0017709145002479279	0.0	0.5071757018287206	3.935365556106507E-4
72-73	1.2024728088103215E-4	0.0017818460712371127	0.0	0.5104333099834977	4.0446812659983544E-4
74-75	1.2024728088103215E-4	0.001825572355193852	0.0	0.5131224764468372	4.0446812659983544E-4
76-77	1.2024728088103215E-4	0.0018966275666235525	0.0	0.5157952455536927	4.0446812659983544E-4
78-79	1.2024728088103215E-4	0.001934888065085699	0.0	0.5175552284829515	4.0446812659983544E-4
80-81	1.2024728088103215E-4	0.001934888065085699	0.0	0.5181455333163676	4.372628395673897E-4
82-83	1.2024728088103215E-4	0.001934888065085699	0.0	0.5185991935124187	4.809891235241286E-4
84-85	1.2024728088103215E-4	0.0019458196360748838	0.0	0.5187686328627511	4.809891235241286E-4
86-87	1.2024728088103215E-4	0.0019676827780532534	0.0	0.5188506196451699	4.809891235241286E-4
88	1.2024728088103215E-4	0.0020660669169559162	0.0	0.5188560854306645	4.919206945133134E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12145	0.0	53.070946	1
GTATCAA	22220	0.0	39.016163	1
TCAACGC	27505	0.0	31.0608	4
ATCAACG	27900	0.0	30.605118	3
CAACGCA	28275	0.0	30.185143	5
TATCAAC	28720	0.0	29.988905	2
AACGCAG	28880	0.0	29.552803	6
ACGCAGA	33065	0.0	25.786152	7
GTACATG	29950	0.0	23.991066	1
CGCAGAG	35485	0.0	23.991058	8
TACATGG	29780	0.0	23.57268	2
ACATGGG	29755	0.0	23.065931	3
GCAGAGT	39000	0.0	21.77299	9
CATGGGG	22295	0.0	20.906725	4
GAGTACT	23740	0.0	20.678797	12-13
GTGGTAT	5785	0.0	20.161865	1
TGGTATC	5315	0.0	19.81579	2
CAGAGTA	38330	0.0	19.331757	10-11
AGAGTAC	35580	0.0	19.29685	10-11
GTACTTT	27225	0.0	17.999935	14-15
>>END_MODULE