FastQCFastQC Report
Fri 27 May 2016
SRR2139223_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139223_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8781492
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121900.051.3253361
GTATCAA216350.037.5821841
TCAACGC261000.030.2325444
ATCAACG263800.029.9444393
TATCAAC271450.029.4868352
CAACGCA268200.029.42115
AACGCAG275450.028.6308576
ACGCAGA314800.025.0518577
CGCAGAG317600.024.8589368
ATCTCGT91600.022.95188194
GTACATG293100.022.329991
TACATGG289050.022.1742882
ACATGGG293650.021.2912983
GTGGTAT57950.021.2612191
GCAGAGT373900.020.929199
GAGTACT210350.020.32442512-13
CATGGGG208200.019.831184
CAGAGTA362250.019.2441310-11
TGGTATC58200.019.1429882
TATCTCG103750.018.97057594
Produced by FastQC (version 0.11.5)