##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139223_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8781492
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54819408820278	33.0	33.0	33.0	33.0	33.0
2	32.56844622758866	33.0	33.0	33.0	33.0	33.0
3	32.56201429096559	33.0	33.0	33.0	33.0	33.0
4	36.47370936510561	37.0	37.0	37.0	37.0	37.0
5	36.50882731544936	37.0	37.0	37.0	37.0	37.0
6	36.50351830873387	37.0	37.0	37.0	37.0	37.0
7	36.485208891609766	37.0	37.0	37.0	37.0	37.0
8	36.47789361989967	37.0	37.0	37.0	37.0	37.0
9	36.49936502817517	37.0	37.0	37.0	37.0	37.0
10-11	36.450982019911876	37.0	37.0	37.0	37.0	37.0
12-13	36.43125029323035	37.0	37.0	37.0	37.0	37.0
14-15	38.401632262490246	40.0	37.0	40.0	37.0	40.0
16-17	38.36910179955753	40.0	37.0	40.0	37.0	40.0
18-19	38.401477049685866	40.0	37.0	40.0	37.0	40.0
20-21	38.40026584319866	40.0	37.0	40.0	37.0	40.0
22-23	38.40186044765673	40.0	37.0	40.0	37.0	40.0
24-25	38.37483327954067	40.0	37.0	40.0	37.0	40.0
26-27	38.255773060543646	40.0	37.0	40.0	37.0	40.0
28-29	38.20289482683805	40.0	37.0	40.0	37.0	40.0
30-31	38.12598854891177	40.0	37.0	40.0	35.0	40.0
32-33	38.04741089277876	40.0	37.0	40.0	33.0	40.0
34-35	38.02660407186966	40.0	37.0	40.0	33.0	40.0
36-37	37.96471882466477	40.0	37.0	40.0	33.0	40.0
38-39	37.914188339875665	40.0	37.0	40.0	33.0	40.0
40-41	37.88326867491886	40.0	37.0	40.0	33.0	40.0
42-43	37.82074072181201	40.0	37.0	40.0	33.0	40.0
44-45	37.74812192803425	40.0	37.0	40.0	33.0	40.0
46-47	37.70498583607743	40.0	37.0	40.0	33.0	40.0
48-49	37.68097758929808	40.0	37.0	40.0	33.0	40.0
50-51	37.5960086972778	40.0	37.0	40.0	33.0	40.0
52-53	37.493327323077324	40.0	37.0	40.0	33.0	40.0
54-55	37.31415586186101	40.0	37.0	40.0	33.0	40.0
56-57	37.19864499223742	38.5	37.0	40.0	33.0	40.0
58-59	37.08244936378577	37.0	37.0	40.0	33.0	40.0
60-61	36.945969525928184	37.0	37.0	40.0	33.0	40.0
62-63	36.78910446077559	37.0	37.0	40.0	33.0	40.0
64-65	36.60292039198129	37.0	37.0	40.0	33.0	40.0
66-67	36.39396680296021	37.0	37.0	40.0	33.0	40.0
68-69	36.17285452084196	37.0	37.0	40.0	33.0	40.0
70-71	35.91327616204203	37.0	37.0	38.5	33.0	40.0
72-73	35.72056235686754	37.0	33.0	37.0	33.0	40.0
74-75	35.51454813600972	37.0	33.0	37.0	33.0	40.0
76-77	33.68614243110044	35.0	33.0	37.0	27.0	37.0
78-79	34.971287487971395	37.0	33.0	37.0	33.0	37.0
80-81	35.01808975851988	37.0	33.0	37.0	33.0	37.0
82-83	34.882324755914055	37.0	33.0	37.0	33.0	37.0
84-85	34.74620183541575	37.0	33.0	37.0	33.0	37.0
86-87	34.64457601237932	37.0	33.0	37.0	33.0	37.0
88-89	34.52634499785029	37.0	33.0	37.0	33.0	37.0
90-91	34.47978297698849	37.0	33.0	37.0	33.0	37.0
92-93	34.43376124529543	37.0	33.0	37.0	33.0	37.0
94-95	34.434266192372256	37.0	33.0	37.0	33.0	37.0
96-97	34.415633889186324	37.0	33.0	37.0	33.0	37.0
98-99	34.42187826934964	37.0	33.0	37.0	33.0	37.0
100	34.39902011131082	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	4.0
18	1.0
19	12.0
20	69.0
21	399.0
22	1537.0
23	4359.0
24	9895.0
25	19044.0
26	33221.0
27	52093.0
28	77640.0
29	107309.0
30	143895.0
31	188270.0
32	247456.0
33	325041.0
34	440704.0
35	629853.0
36	1073600.0
37	2496747.0
38	2860819.0
39	69523.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.638459614835384	20.09140360203027	14.422469439134034	23.847667344000314
2	14.455311238682448	20.84164057770593	42.1105661771371	22.592482006474526
3	18.013886478516408	26.316427777876473	30.627551673451393	25.04213407015573
4	12.3469218217321	18.088172711825784	38.88724083085356	30.67766463558856
5	13.249525251517625	37.853430829294155	35.589009248086775	13.30803467110145
6	29.794195260274492	37.94749153303219	18.76543803156621	13.492875175127105
7	26.31131475152514	32.85315297218286	23.02707785875111	17.808454417540894
8	24.398564617493246	36.917769782173686	21.049338768400634	17.63432683193243
9	25.71657526989719	16.809876954850044	21.021541669684378	36.452006105568394
10-11	24.2233097551468	26.766322683196336	28.82046964522484	20.189897916432027
12-13	25.14049438079415	24.325160081034085	28.91733065800906	21.617014880162706
14-15	22.259927036458542	25.620204906477028	26.617640159191524	25.50222789787291
16-17	21.72157647014881	28.833824593816175	27.25822673413584	22.186372201899175
18-19	21.750797017181135	27.840570827827438	29.082153693244837	21.326478461746593
20-21	22.605391429070057	26.996342492144485	28.965423782085757	21.432842296699704
22-23	22.27718786187996	27.04277962074297	28.80941436832558	21.870618149051495
24-25	22.063217809466266	27.186063868468462	28.90704096873465	21.84367735333062
26-27	21.979491538749915	27.342970342609124	28.898476393847233	21.779061724793724
28-29	21.882847911787163	27.391008999071843	28.90266999139786	21.82347309774313
30-31	22.129507081946116	27.137619316016586	28.898727143694934	21.834146458342367
32-33	21.874221103029534	27.41817331982531	28.762781624288387	21.94482395285677
34-35	22.05154827073879	27.3712010425161	28.76215013333503	21.815100553410083
36-37	22.038967413172223	27.38281249551312	28.771358546996815	21.80686154431784
38-39	21.843467846895066	27.45271615773264	28.761221732073793	21.942594263298503
40-41	22.150706302934253	27.30253067517932	28.69690407192062	21.849858949965807
42-43	22.08104820284918	27.33746413178259	28.52817295747025	22.053314707897982
44-45	22.082069092215395	27.530520233513574	28.303364041764716	22.084046632506315
46-47	22.232095250830948	27.425367889496627	28.04037303192549	22.30216382774693
48-49	22.07084805026672	27.646243794673808	27.976191535984334	22.30671661907514
50-51	22.053624880017928	27.78763925445564	27.832699719687476	22.326036145838955
52-53	22.14083768545587	27.64509076206263	27.82147515800491	22.39259639447659
54-55	22.22982464337211	27.406777559787947	27.926448494639644	22.436949302200297
56-57	22.181375582439614	27.409417708182886	27.87950285989896	22.529703849478548
58-59	21.96226406464712	27.39433633588887	28.023599583407904	22.619800016056104
60-61	22.188001982795154	27.553437437141014	28.060964231903256	22.197596348160573
62-63	21.784102236195714	27.736632817438284	28.054866181050226	22.42439876531578
64-65	21.72942007051581	27.90119134080023	27.823580563090466	22.54580802559349
66-67	21.845222440330545	28.23645456837233	27.46511499354392	22.4532079977532
68-69	21.87723248611905	28.187643232308584	27.365031875244583	22.570092406327788
70-71	21.885858451223815	27.740454777078575	27.38372224255946	22.98996452913815
72-73	22.072539558583422	27.477203218342243	27.32646781972517	23.123789403349168
74-75	22.04683286238227	27.528926407929706	27.454043232929255	22.97019749675877
76-77	22.349210945436422	27.389396798497174	27.051936164537743	23.20945609152866
78-79	22.48547653328136	27.479563493798913	26.70086208055702	23.334097892362706
80-81	22.367108562681658	27.654398095022913	26.854565297257864	23.123928045037566
82-83	22.341653258081486	27.529968512432283	26.935168578021123	23.193209651465107
84-85	22.500007732742436	27.69229548543669	26.881493906666204	22.926202875154672
86-87	22.647786993429094	27.632548825194714	27.023654018429855	22.696010162946344
88-89	22.556641994883485	27.70253749044931	27.19168987306019	22.549130641607015
90-91	22.526262701327894	27.428786698407638	27.18366676102118	22.861283839243292
92-93	22.53391449057631	27.10479183969379	27.344164187615068	23.017129482114836
94-95	22.080277365165397	27.360605543226256	27.728519803331782	22.830597288276564
96-97	22.154015285380183	27.375032595626962	27.818537300772906	22.65241481821995
98-99	23.32734410009483	29.237968321753694	28.18806467419798	19.246622903953497
100	22.367555797132862	30.36025956962929	21.874860361276642	25.397324271961214
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1321.0
1	1240.0
2	1986.5
3	3049.0
4	4276.0
5	7107.5
6	9568.0
7	11272.5
8	12448.5
9	12451.5
10	11638.0
11	10738.0
12	11593.5
13	15090.0
14	23485.0
15	35267.0
16	45180.0
17	51169.0
18	52555.0
19	49967.0
20	44791.5
21	38932.5
22	35395.5
23	35839.5
24	40814.5
25	50080.0
26	63251.5
27	78719.5
28	94009.5
29	111104.0
30	128712.5
31	145843.0
32	166064.0
33	187073.5
34	207445.0
35	226328.0
36	247937.0
37	268865.0
38	282794.5
39	294481.0
40	308266.0
41	320525.0
42	327890.0
43	336636.0
44	345624.5
45	352069.5
46	355673.5
47	358304.0
48	363930.0
49	362190.0
50	352815.0
51	342951.0
52	330658.5
53	313252.0
54	292085.0
55	269535.0
56	245547.0
57	221518.0
58	196987.5
59	171006.5
60	142170.5
61	114580.5
62	89746.0
63	67486.5
64	49105.0
65	35695.0
66	26502.5
67	19994.5
68	15193.5
69	10685.0
70	7232.5
71	5128.5
72	3557.0
73	2344.0
74	1548.5
75	1038.5
76	692.0
77	447.5
78	292.5
79	202.0
80	148.5
81	121.5
82	99.5
83	72.5
84	48.5
85	33.0
86	25.0
87	20.5
88	15.5
89	10.0
90	5.0
91	3.0
92	2.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.001286797277729115
5	0.0
6	1.4803862487149108E-4
7	0.0
8	0.0
9	0.0
10-11	0.004019818044587412
12-13	2.2775173057152475E-5
14-15	0.0013665103834291485
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	139.0
20-21	571.0
22-23	1134.0
24-25	2956.0
26-27	3196.0
28-29	5976.0
30-31	13343.0
32-33	16910.0
34-35	24003.0
36-37	32487.0
38-39	26369.0
40-41	42935.0
42-43	50800.0
44-45	61348.0
46-47	60435.0
48-49	54382.0
50-51	31575.0
52-53	13134.0
54-55	13585.0
56-57	14478.0
58-59	36097.0
60-61	61788.0
62-63	61242.0
64-65	65630.0
66-67	71729.0
68-69	67983.0
70-71	67825.0
72-73	65912.0
74-75	68446.0
76-77	66860.0
78-79	73166.0
80-81	75950.0
82-83	74474.0
84-85	76378.0
86-87	81131.0
88-89	88015.0
90-91	93452.0
92-93	99273.0
94-95	113060.0
96-97	312914.0
98-99	592791.0
100-101	5997620.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.59357722409668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.96577934631759	72.65408389517931
2	8.334393868219344	13.767348071416247
3	2.0250134411352536	5.017593120907151
4	0.7612725469484795	2.5150489157989604
5	0.3424678688113639	1.4142823184721596
6	0.19439117243369872	0.9633277387251236
7	0.11030374448704433	0.6377266586878407
8	0.06936836643692172	0.4583505224173865
9	0.045115205936939226	0.33536036209802533
>10	0.14752990672048646	1.8626664602241203
>50	0.0034812125078845244	0.19437134944392218
>100	7.876367712397622E-4	0.10668019623413251
>500	7.176245822859177E-5	0.046033950821076204
>1k	2.392081550692767E-5	0.027126439574521807
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.416275958572871E-5
2	0.0	4.555034611430495E-5	0.0	0.0	3.416275958572871E-5
3	0.0	4.555034611430495E-5	0.0	1.1387586528576238E-5	3.416275958572871E-5
4	0.0	1.3665103834291484E-4	0.0	2.2775173057152475E-5	4.555034611430495E-5
5	0.0	1.3665103834291484E-4	0.0	3.416275958572871E-5	4.555034611430495E-5
6	3.416275958572871E-5	1.4803862487149108E-4	0.0	3.416275958572871E-5	5.6937932642881185E-5
7	3.416275958572871E-5	1.4803862487149108E-4	0.0	3.416275958572871E-5	5.6937932642881185E-5
8	3.416275958572871E-5	1.4803862487149108E-4	0.0	3.416275958572871E-5	5.6937932642881185E-5
9	3.416275958572871E-5	2.733020766858297E-4	0.0	5.6937932642881185E-5	7.971310570003366E-5
10-11	3.416275958572871E-5	2.733020766858297E-4	0.0	7.971310570003366E-5	7.971310570003366E-5
12-13	3.985655285001683E-5	2.733020766858297E-4	0.0	9.11006922286099E-5	8.540689896432178E-5
14-15	4.555034611430495E-5	3.587089756501515E-4	0.0	1.1387586528576238E-4	1.537324181357792E-4
16-17	1.0818207202147425E-4	3.644027689144396E-4	0.0	1.4803862487149108E-4	1.7081379792864356E-4
18-19	1.1956965855005049E-4	3.757903554430158E-4	0.0	2.106703507786604E-4	1.765075911929317E-4
20-21	1.2526345181433862E-4	3.985655285001683E-4	0.0	2.9607724974298216E-4	1.9358897098579604E-4
22-23	1.2526345181433862E-4	4.6119725440733764E-4	0.0	5.181351870502188E-4	2.2775173057152477E-4
24-25	1.2526345181433862E-4	5.352165668430831E-4	0.0	9.337820953432515E-4	2.733020766858297E-4
26-27	1.2526345181433862E-4	5.693793264288119E-4	0.0	0.002197804200015214	2.8468966321440594E-4
28-29	1.2526345181433862E-4	5.693793264288119E-4	0.0	0.007703702286581825	2.8468966321440594E-4
30-31	1.2526345181433862E-4	6.263172590716931E-4	0.0	0.012628833460191047	2.960772497429822E-4
32-33	1.2526345181433862E-4	6.433986388645574E-4	0.0	0.022815029610002494	3.416275958572871E-4
34-35	1.2526345181433862E-4	7.288055378288792E-4	0.0	0.035204723753093435	3.644027689144396E-4
36-37	1.2526345181433862E-4	8.085186435289128E-4	0.0	0.05598706916774507	3.8717794197159203E-4
38-39	1.2526345181433862E-4	8.426814031146416E-4	0.0	0.15803123205031674	4.0995311502874457E-4
40-41	1.2526345181433862E-4	9.451696818718277E-4	0.0	0.2686502475889063	4.0995311502874457E-4
42-43	1.2526345181433862E-4	9.508634751361158E-4	0.0	0.31002134944722376	4.156469082930327E-4
44-45	1.2526345181433862E-4	0.001002107614514709	0.0	0.3657237289517544	4.213407015573208E-4
46-47	1.2526345181433862E-4	0.00105904554715759	0.0	0.4230943898827215	4.32728288085897E-4
48-49	1.2526345181433862E-4	0.0010761269269504545	0.0	0.4730118754307354	4.7258484093591385E-4
50-51	1.3095724507862673E-4	0.001246940724879098	0.0	0.5163871925180823	4.953600139930663E-4
52-53	1.3665103834291484E-4	0.0012754096912005386	0.0	0.8412010168659266	5.124413937859307E-4
54-55	1.3665103834291484E-4	0.0012811034844648268	0.0	1.28527703492755	5.238289803145069E-4
56-57	1.3665103834291484E-4	0.001343735210371996	0.0	1.7839565303936962	5.29522773578795E-4
58-59	1.3665103834291484E-4	0.001343735210371996	0.0	2.3115889646087475	5.466041533716594E-4
60-61	1.4803862487149108E-4	0.0013608165901648605	0.0	2.4588418460097667	5.466041533716594E-4
62-63	1.7081379792864356E-4	0.0013665103834291485	0.0	2.465560522061627	5.693793264288119E-4
64-65	1.7081379792864356E-4	0.001491773835243487	0.0	2.4713283346383506	5.693793264288119E-4
66-67	1.822013844572198E-4	0.001491773835243487	0.0	2.475023606466874	5.750731196931E-4
68-69	1.9358897098579604E-4	0.0015259365948292157	0.0	2.4788783045067966	5.807669129573881E-4
70-71	1.9358897098579604E-4	0.0015430179746220803	0.0	2.4829038163446486	6.035420860145405E-4
72-73	1.9358897098579604E-4	0.0015544055611506563	0.0	2.4861720536783496	6.604800186574218E-4
74-75	1.9358897098579604E-4	0.0016227310803221138	0.0	2.488705791680958	6.71867605185998E-4
76-77	1.9928276425008418E-4	0.001679669012964995	0.0	2.491461587620874	7.060303647717268E-4
78-79	2.0497655751437228E-4	0.0017081379792864356	0.0	2.493164031806896	7.401931243574554E-4
80-81	2.0497655751437228E-4	0.0017195255658150118	0.0	2.493784655272703	7.572745041503199E-4
82-83	2.106703507786604E-4	0.0017366069456078762	0.0	2.494297096666489	7.800496772074723E-4
84-85	2.163641440429485E-4	0.0017423007388721644	0.0	2.494519154603796	7.857434704717604E-4
86-87	2.163641440429485E-4	0.0017536883254007406	0.0	2.4946273366758174	7.857434704717604E-4
88	2.2775173057152474E-4	0.001867564190686503	0.0	2.494633030469082	7.857434704717604E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12190	0.0	51.325336	1
GTATCAA	21635	0.0	37.582184	1
TCAACGC	26100	0.0	30.232544	4
ATCAACG	26380	0.0	29.944439	3
TATCAAC	27145	0.0	29.486835	2
CAACGCA	26820	0.0	29.4211	5
AACGCAG	27545	0.0	28.630857	6
ACGCAGA	31480	0.0	25.051857	7
CGCAGAG	31760	0.0	24.858936	8
ATCTCGT	9160	0.0	22.951881	94
GTACATG	29310	0.0	22.32999	1
TACATGG	28905	0.0	22.174288	2
ACATGGG	29365	0.0	21.291298	3
GTGGTAT	5795	0.0	21.261219	1
GCAGAGT	37390	0.0	20.92919	9
GAGTACT	21035	0.0	20.324425	12-13
CATGGGG	20820	0.0	19.83118	4
CAGAGTA	36225	0.0	19.24413	10-11
TGGTATC	5820	0.0	19.142988	2
TATCTCG	10375	0.0	18.970575	94
>>END_MODULE