FastQCFastQC Report
Fri 27 May 2016
SRR2139222_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139222_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6166846
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81300.051.8907241
GTATCAA149350.036.1943931
TCAACGC175000.030.3491974
ATCAACG176300.030.051743
CAACGCA179750.029.5737655
TATCAAC183000.029.1159822
AACGCAG183950.028.8985256
ACGCAGA212500.025.0361637
CGCAGAG215200.024.7026638
GTGGTAT39350.021.6387271
GTACATG180300.021.609451
TGGTATC37250.021.4732572
TACATGG179400.021.0466672
GCAGAGT251900.020.8651359
ACATGGG179050.020.6314663
GAGTACT156700.019.78785112-13
CATGGGG128650.019.0538774
CAGAGTA246250.018.9944910-11
AGAGTAC229750.018.41168210-11
GTACTTT173950.017.71437614-15
Produced by FastQC (version 0.11.5)