##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139222_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6166846 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.521587372215876 33.0 33.0 33.0 33.0 33.0 2 32.54151068471631 33.0 33.0 33.0 33.0 33.0 3 32.5375339354996 33.0 33.0 33.0 33.0 33.0 4 36.44414243520918 37.0 37.0 37.0 37.0 37.0 5 36.48184533876799 37.0 37.0 37.0 37.0 37.0 6 36.47890493779154 37.0 37.0 37.0 37.0 37.0 7 36.46020121144585 37.0 37.0 37.0 37.0 37.0 8 36.4529007210493 37.0 37.0 37.0 37.0 37.0 9 36.47676591891544 37.0 37.0 37.0 37.0 37.0 10-11 36.427832071694354 37.0 37.0 37.0 37.0 37.0 12-13 36.41030366252052 37.0 37.0 37.0 37.0 37.0 14-15 38.354214699053614 40.0 37.0 40.0 37.0 40.0 16-17 38.32141527451796 40.0 37.0 40.0 37.0 40.0 18-19 38.34882620710814 40.0 37.0 40.0 37.0 40.0 20-21 38.34742485294895 40.0 37.0 40.0 37.0 40.0 22-23 38.350136162635394 40.0 37.0 40.0 37.0 40.0 24-25 38.32462991731215 40.0 37.0 40.0 37.0 40.0 26-27 38.201790665387016 40.0 37.0 40.0 37.0 40.0 28-29 38.14587355600298 40.0 37.0 40.0 35.0 40.0 30-31 38.06728563201695 40.0 37.0 40.0 33.0 40.0 32-33 37.99106400951711 40.0 37.0 40.0 33.0 40.0 34-35 37.97038059122377 40.0 37.0 40.0 33.0 40.0 36-37 37.91342958566388 40.0 37.0 40.0 33.0 40.0 38-39 37.86275790229941 40.0 37.0 40.0 33.0 40.0 40-41 37.83510761059395 40.0 37.0 40.0 33.0 40.0 42-43 37.776286852730635 40.0 37.0 40.0 33.0 40.0 44-45 37.705840976530006 40.0 37.0 40.0 33.0 40.0 46-47 37.66447161573612 40.0 37.0 40.0 33.0 40.0 48-49 37.64872279574053 40.0 37.0 40.0 33.0 40.0 50-51 37.56946571498953 40.0 37.0 40.0 33.0 40.0 52-53 37.46809353031018 40.0 37.0 40.0 33.0 40.0 54-55 37.29080876979887 40.0 37.0 40.0 33.0 40.0 56-57 37.18033906589955 38.5 37.0 40.0 33.0 40.0 58-59 37.06771204192037 37.0 37.0 40.0 33.0 40.0 60-61 36.93429097537671 37.0 37.0 40.0 33.0 40.0 62-63 36.77483419122305 37.0 37.0 40.0 33.0 40.0 64-65 36.588426196018396 37.0 37.0 40.0 33.0 40.0 66-67 36.38576594030397 37.0 37.0 40.0 33.0 40.0 68-69 36.17133068678781 37.0 37.0 40.0 33.0 40.0 70-71 35.91098311791709 37.0 35.0 38.5 33.0 40.0 72-73 35.71407217759247 37.0 33.0 37.0 33.0 40.0 74-75 35.515599973176165 37.0 33.0 37.0 33.0 40.0 76-77 33.70286682604784 35.0 33.0 37.0 27.0 37.0 78-79 34.9854605089057 37.0 33.0 37.0 33.0 37.0 80-81 35.03883705152457 37.0 33.0 37.0 33.0 37.0 82-83 34.901557912724186 37.0 33.0 37.0 33.0 37.0 84-85 34.75704700482932 37.0 33.0 37.0 33.0 37.0 86-87 34.65806867552414 37.0 33.0 37.0 33.0 37.0 88-89 34.539131554498724 37.0 33.0 37.0 33.0 37.0 90-91 34.48029902878568 37.0 33.0 37.0 33.0 37.0 92-93 34.42305944655264 37.0 33.0 37.0 33.0 37.0 94-95 34.4400129394657 37.0 33.0 37.0 33.0 37.0 96-97 34.42516353886893 37.0 33.0 37.0 33.0 37.0 98-99 34.42625621953624 37.0 33.0 37.0 33.0 37.0 100 34.40837944074328 37.0 33.0 37.0 33.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 1.0 17 0.0 18 0.0 19 8.0 20 51.0 21 286.0 22 1090.0 23 3014.0 24 6814.0 25 13767.0 26 23576.0 27 37165.0 28 54154.0 29 75104.0 30 101866.0 31 134180.0 32 175158.0 33 230924.0 34 314478.0 35 447798.0 36 754053.0 37 1719167.0 38 2021331.0 39 52860.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.52242491542678 20.291507198331203 14.42082387009502 23.765244016146987 2 14.465919855952297 21.270905743389733 41.87004183337804 22.393132567279935 3 17.929375891663256 26.427431461722893 30.678778098236926 24.96441454837692 4 12.26530854822308 18.46135908815754 39.3476679109167 29.925664452702687 5 13.199032374085554 37.839213108289066 35.77995948009728 13.181795037528099 6 29.316758442495168 38.34449680695358 19.054189521437078 13.284555229114176 7 25.9414455947173 32.940696102999816 23.41514608926508 17.702712213017804 8 24.05184108699974 36.938314983056166 21.404393753305982 17.605450176638108 9 25.418925655026896 16.97927919717794 21.33526603388507 36.266529113910096 10-11 23.757366497362163 27.001714558798273 29.197843350007563 20.043075593832 12-13 24.773575466871634 24.54760092073013 29.255291806426136 21.4235318059721 14-15 21.95914952063544 25.97285526469948 26.897409213837253 25.17058600082783 16-17 21.482399592919947 29.081300230296 27.52476711758328 21.911533059200767 18-19 21.489250745032386 28.191136928017986 29.297983118112565 21.021629208837062 20-21 22.195344648130764 27.32094415366412 29.315367539023857 21.16834365918126 22-23 21.889875235137275 27.282881065909148 29.165033402912936 21.662210296040644 24-25 21.769589173488686 27.446636253817896 29.166365895996233 21.617408676697185 26-27 21.714665568198203 27.642354721960714 29.084078354640504 21.55890135520058 28-29 21.612643244087504 27.68719754800997 29.080250459390943 21.619908748511588 30-31 22.02885537006571 27.531577448295952 28.982285261771736 21.4572819198666 32-33 21.809466658753482 27.647214319215056 28.939945205274253 21.603373816757205 34-35 21.8824365599874 27.73753946512666 28.980781737958907 21.399242236927034 36-37 21.846498879052454 27.69989030417625 28.901017818079815 21.552592998691477 38-39 21.675804603500385 27.74109527114339 29.006557455512848 21.576542669843377 40-41 21.933106209122375 27.589310633143654 28.852997941237458 21.624585216496513 42-43 21.829114777738912 27.721845345585304 28.636654548827178 21.8123853278486 44-45 21.821664409288015 27.805315900814563 28.550518594675534 21.822501095221888 46-47 21.939054359363748 27.811905761619805 28.219946111283377 22.02909376773307 48-49 21.8223208571381 27.982389147032304 28.205711240335766 21.98957875549383 50-51 21.873569696882058 28.043152381440517 28.11009131607011 21.973186605607314 52-53 22.036346219797565 27.952316891602475 28.029970704860073 21.98136618373989 54-55 21.9168029605066 27.90453114514887 28.030824522013088 22.147841372331445 56-57 21.95240581172521 27.9780679546016 27.904848943608457 22.164677290064738 58-59 21.90560072878316 28.0443446142366 27.985260971929872 22.064793685050372 60-61 22.11438806473141 28.052348619454236 27.912006784693165 21.921256531121188 62-63 21.996176403366885 28.157474610642417 27.847943236203086 21.99840574978761 64-65 21.937133518693322 28.090891588495076 27.79612462562034 22.17585026719126 66-67 22.03054371977448 28.131807513418504 27.61312859875091 22.224520168056102 68-69 22.01464025163447 28.09046361711744 27.719763882255855 22.175132248992234 70-71 22.028917913461918 27.925425575825493 27.76997833542944 22.27567817528315 72-73 22.073374521792157 27.961270019808726 27.634468464059697 22.33088699433942 74-75 21.98419915730844 28.096118942214577 27.626136821591192 22.293545078885796 76-77 22.129612282503267 28.009628313883034 27.55576003206276 22.30499937155094 78-79 22.094714876093597 28.010686550854523 27.54731286447038 22.3472857085815 80-81 22.117183032325315 28.083181597639502 27.493134169433763 22.30650120060142 82-83 22.15799307666263 28.07849628928196 27.60546868585611 22.1580419481993 84-85 22.225818002766324 28.07476269658918 27.5225541686179 22.176865132026595 86-87 22.06843453024867 28.108036721512825 27.6053504474395 22.21817830079901 88-89 22.101683036888666 28.062722699564308 27.57638749686321 22.259206766683818 90-91 22.168404572759755 28.057129232158516 27.606644423431792 22.167821771649937 92-93 22.22265734103452 28.006287674037168 27.612981707605133 22.158073277323183 94-95 22.111661892635155 28.08890838483598 27.645299687831304 22.154130034697555 96-97 22.08874942432974 28.185373653167545 27.73068262963386 21.995194292868852 98-99 23.1351591814 30.07431792445872 27.95480757306677 18.835715321074513 100 22.076559428339625 31.10668022747839 21.895599627110183 24.9211607170718 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2480.0 1 2008.0 2 2177.0 3 2993.0 4 3778.5 5 5646.5 6 7368.5 7 8869.0 8 10299.5 9 10512.5 10 9600.0 11 8624.5 12 9105.5 13 11454.5 14 17049.5 15 24807.5 16 31225.5 17 35152.0 18 36099.0 19 34370.5 20 31385.5 21 28738.5 22 27694.0 23 29319.5 24 33970.0 25 41797.0 26 52261.0 27 64161.0 28 77072.5 29 91274.0 30 105562.0 31 119308.5 32 133614.5 33 147602.0 34 160099.0 35 171328.5 36 184227.5 37 196995.0 38 207120.0 39 216052.0 40 225232.5 41 233223.5 42 238486.5 43 243898.0 44 248185.0 45 251178.5 46 252569.5 47 252632.5 48 254247.5 49 251915.0 50 245229.0 51 236746.0 52 225962.0 53 213816.5 54 199714.5 55 183346.0 56 165897.0 57 148379.5 58 130710.0 59 112608.0 60 93060.5 61 74245.5 62 57893.0 63 43910.5 64 32168.0 65 23273.5 66 17149.0 67 12697.0 68 9417.5 69 6675.0 70 4523.5 71 3217.5 72 2375.5 73 1666.0 74 1127.0 75 771.5 76 529.5 77 349.0 78 222.0 79 142.0 80 102.5 81 74.5 82 52.0 83 41.5 84 35.0 85 28.0 86 20.0 87 17.0 88 14.5 89 9.0 90 5.0 91 4.5 92 3.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0012810438269416813 5 0.0 6 1.2972595715865127E-4 7 0.0 8 0.0 9 0.0 10-11 0.003972857437983696 12-13 1.621574464483141E-5 14-15 0.0014350934010675799 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 122.0 20-21 445.0 22-23 963.0 24-25 2150.0 26-27 2822.0 28-29 5045.0 30-31 10695.0 32-33 13297.0 34-35 18464.0 36-37 26011.0 38-39 29056.0 40-41 32513.0 42-43 38250.0 44-45 46374.0 46-47 45447.0 48-49 42167.0 50-51 36098.0 52-53 34722.0 54-55 37786.0 56-57 41168.0 58-59 42390.0 60-61 43206.0 62-63 43453.0 64-65 46898.0 66-67 50128.0 68-69 49026.0 70-71 48208.0 72-73 47647.0 74-75 49836.0 76-77 47836.0 78-79 52177.0 80-81 53733.0 82-83 52461.0 84-85 53604.0 86-87 56484.0 88-89 60300.0 90-91 63486.0 92-93 66020.0 94-95 81165.0 96-97 211949.0 98-99 381228.0 100-101 4102016.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.08815149200294 #Duplication Level Percentage of deduplicated Percentage of total 1 91.22634170322067 79.44733466311305 2 6.369486867349969 11.094136744601945 3 1.3707707167560343 3.581336635249529 4 0.46759182988347925 1.6288683246926121 5 0.21675770937777555 0.9438514115675631 6 0.11070682839706869 0.5784751825585854 7 0.06947655063138503 0.423540905656951 8 0.03875829841880929 0.27003108514156166 9 0.029437435220664256 0.23072866362299083 >10 0.09672977585846819 1.334665812808757 >50 0.0026205647391513097 0.15820395999816997 >100 0.0010925232823903411 0.18105555487642708 >500 2.291968640773549E-4 0.12777105611181766 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.621574464483141E-5 0.0 0.0 0.0 3 0.0 1.621574464483141E-5 0.0 1.621574464483141E-5 0.0 4 0.0 3.243148928966282E-5 0.0 4.864723393449423E-5 0.0 5 0.0 8.107872322415705E-5 0.0 8.107872322415705E-5 0.0 6 0.0 8.107872322415705E-5 0.0 8.107872322415705E-5 0.0 7 0.0 8.107872322415705E-5 0.0 8.107872322415705E-5 1.621574464483141E-5 8 0.0 1.1351021251381987E-4 0.0 8.107872322415705E-5 1.621574464483141E-5 9 0.0 1.459417018034827E-4 0.0 1.1351021251381987E-4 3.243148928966282E-5 10-11 0.0 1.459417018034827E-4 0.0 1.2161808483623557E-4 3.243148928966282E-5 12-13 0.0 1.621574464483141E-4 0.0 1.2972595715865127E-4 3.243148928966282E-5 14-15 0.0 1.9458893573797692E-4 0.0 1.3783382948106697E-4 3.243148928966282E-5 16-17 0.0 1.9458893573797692E-4 0.0 1.540495741258984E-4 3.243148928966282E-5 18-19 0.0 2.4323616967247114E-4 0.0 1.7837319109314552E-4 3.243148928966282E-5 20-21 0.0 3.080991482517968E-4 0.0 1.7837319109314552E-4 3.243148928966282E-5 22-23 0.0 3.405306375414596E-4 0.0 1.8648106341556122E-4 4.0539361612078524E-5 24-25 0.0 3.8917787147595384E-4 0.0 2.999912759293811E-4 6.486297857932564E-5 26-27 0.0 4.4593297773286376E-4 0.0 7.621399983070763E-4 8.107872322415705E-5 28-29 0.0 4.540408500552795E-4 8.107872322415704E-6 0.0032836882905783607 1.1351021251381987E-4 30-31 0.0 5.594431902466836E-4 1.621574464483141E-5 0.010126732530697215 1.7026531877072982E-4 32-33 1.621574464483141E-5 5.675510625690993E-4 1.621574464483141E-5 0.023318240799267566 2.0269680806039262E-4 34-35 1.621574464483141E-5 6.243061688260092E-4 1.621574464483141E-5 0.03886913991366089 2.2702042502763974E-4 36-37 1.621574464483141E-5 6.891691474053349E-4 1.621574464483141E-5 0.060403648801997 2.2702042502763974E-4 38-39 1.621574464483141E-5 7.216006366949977E-4 1.621574464483141E-5 0.10339969572776748 2.2702042502763974E-4 40-41 1.621574464483141E-5 8.51326593853649E-4 1.621574464483141E-5 0.15011725604952678 2.2702042502763974E-4 42-43 1.621574464483141E-5 8.756502108208961E-4 1.621574464483141E-5 0.18777832298714775 2.4323616967247114E-4 44-45 1.621574464483141E-5 9.405131894002218E-4 1.621574464483141E-5 0.23426075501155696 2.5945191431730254E-4 46-47 1.621574464483141E-5 9.89160423334716E-4 1.621574464483141E-5 0.27876486618929674 2.5945191431730254E-4 48-49 1.621574464483141E-5 0.001013484040301963 1.621574464483141E-5 0.3180475075914009 2.918834036069654E-4 50-51 1.621574464483141E-5 0.0010621312742364573 1.621574464483141E-5 0.3473574660369336 2.918834036069654E-4 52-53 1.621574464483141E-5 0.001070239146558873 1.621574464483141E-5 0.40674763079862863 2.999912759293811E-4 54-55 1.621574464483141E-5 0.001070239146558873 1.621574464483141E-5 0.46782423300338616 3.080991482517968E-4 56-57 1.621574464483141E-5 0.0010864548912037045 1.621574464483141E-5 0.5272225056374036 3.080991482517968E-4 58-59 1.621574464483141E-5 0.0010864548912037045 1.621574464483141E-5 0.5642511585338762 3.080991482517968E-4 60-61 1.621574464483141E-5 0.0010945627635261202 1.621574464483141E-5 0.5896125831583925 3.080991482517968E-4 62-63 1.621574464483141E-5 0.001102670635848536 1.621574464483141E-5 0.6098336167304972 3.080991482517968E-4 64-65 1.621574464483141E-5 0.0012486123376520186 2.432361696724711E-5 0.6175360954367921 3.080991482517968E-4 66-67 1.621574464483141E-5 0.0012972595715865128 3.243148928966282E-5 0.6247845332930317 3.080991482517968E-4 68-69 1.621574464483141E-5 0.0013377989331985914 3.243148928966282E-5 0.6322194522126869 3.1620702057421246E-4 70-71 1.621574464483141E-5 0.0013459068055210071 3.243148928966282E-5 0.6372057936909726 3.243148928966282E-4 72-73 1.621574464483141E-5 0.0013540146778434226 3.243148928966282E-5 0.6409029834699942 3.5674638218629104E-4 74-75 1.621574464483141E-5 0.001370230422488254 3.243148928966282E-5 0.6439353277185778 3.5674638218629104E-4 76-77 1.621574464483141E-5 0.001402661911777917 3.243148928966282E-5 0.6472838789877353 3.5674638218629104E-4 78-79 1.621574464483141E-5 0.0014269855287451642 3.243148928966282E-5 0.6493108470683393 3.5674638218629104E-4 80-81 1.621574464483141E-5 0.0014432012733899954 4.0539361612078524E-5 0.6500567713220016 3.8106999915353817E-4 82-83 2.432361696724711E-5 0.0014432012733899954 4.864723393449423E-5 0.6505513515336689 4.0539361612078524E-4 84-85 3.243148928966282E-5 0.0014432012733899954 4.864723393449423E-5 0.6507702640863742 4.2160936076561663E-4 86-87 3.243148928966282E-5 0.0014432012733899954 4.864723393449423E-5 0.6508432349372759 4.2160936076561663E-4 88 3.243148928966282E-5 0.0015080642519693212 4.864723393449423E-5 0.6508513428095983 4.2160936076561663E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8130 0.0 51.890724 1 GTATCAA 14935 0.0 36.194393 1 TCAACGC 17500 0.0 30.349197 4 ATCAACG 17630 0.0 30.05174 3 CAACGCA 17975 0.0 29.573765 5 TATCAAC 18300 0.0 29.115982 2 AACGCAG 18395 0.0 28.898525 6 ACGCAGA 21250 0.0 25.036163 7 CGCAGAG 21520 0.0 24.702663 8 GTGGTAT 3935 0.0 21.638727 1 GTACATG 18030 0.0 21.60945 1 TGGTATC 3725 0.0 21.473257 2 TACATGG 17940 0.0 21.046667 2 GCAGAGT 25190 0.0 20.865135 9 ACATGGG 17905 0.0 20.631466 3 GAGTACT 15670 0.0 19.787851 12-13 CATGGGG 12865 0.0 19.053877 4 CAGAGTA 24625 0.0 18.99449 10-11 AGAGTAC 22975 0.0 18.411682 10-11 GTACTTT 17395 0.0 17.714376 14-15 >>END_MODULE