FastQCFastQC Report
Fri 27 May 2016
SRR2139221_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139221_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10913760
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154800.048.2076151
GTATCAA270050.036.638761
TCAACGC331650.029.3561524
ATCAACG335000.029.0615233
CAACGCA339650.028.6786485
TATCAAC346000.028.2987422
AACGCAG344800.028.2254186
CTTATAC110150.027.3331661
ACGCAGA394750.024.6863777
CGCAGAG404300.024.1140888
TATACAC159850.021.3300483
GCAGAGT471100.020.4675719
GAGTACT288850.020.19341912-13
GTGGTAT73850.020.1519171
GTACATG314700.020.0062961
TTATACA165850.019.4205632
TACATGG310450.019.3407692
AGAGTAC420700.018.98376510-11
CAGAGTA450600.018.82350310-11
ACATGGG311500.018.6422353
Produced by FastQC (version 0.11.5)