##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139221_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10913760
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52654914529914	33.0	33.0	33.0	33.0	33.0
2	32.547828062922406	33.0	33.0	33.0	33.0	33.0
3	32.54260135828532	33.0	33.0	33.0	33.0	33.0
4	36.449929538490856	37.0	37.0	37.0	37.0	37.0
5	36.48595635234786	37.0	37.0	37.0	37.0	37.0
6	36.485126390904696	37.0	37.0	37.0	37.0	37.0
7	36.46656935831464	37.0	37.0	37.0	37.0	37.0
8	36.46165006377271	37.0	37.0	37.0	37.0	37.0
9	36.481397795077044	37.0	37.0	37.0	37.0	37.0
10-11	36.43543416750964	37.0	37.0	37.0	37.0	37.0
12-13	36.416670744088194	37.0	37.0	37.0	37.0	37.0
14-15	38.37039622458255	40.0	37.0	40.0	37.0	40.0
16-17	38.33577021118295	40.0	37.0	40.0	37.0	40.0
18-19	38.36844671314011	40.0	37.0	40.0	37.0	40.0
20-21	38.36916800823421	40.0	37.0	40.0	37.0	40.0
22-23	38.36957068997468	40.0	37.0	40.0	37.0	40.0
24-25	38.34471637231145	40.0	37.0	40.0	37.0	40.0
26-27	38.22075177330345	40.0	37.0	40.0	37.0	40.0
28-29	38.16585512801784	40.0	37.0	40.0	37.0	40.0
30-31	38.09122573764044	40.0	37.0	40.0	33.0	40.0
32-33	38.011317192684054	40.0	37.0	40.0	33.0	40.0
34-35	37.98623905700654	40.0	37.0	40.0	33.0	40.0
36-37	37.93133467118221	40.0	37.0	40.0	33.0	40.0
38-39	37.87796311579733	40.0	37.0	40.0	33.0	40.0
40-41	37.85096876461999	40.0	37.0	40.0	33.0	40.0
42-43	37.79429756944934	40.0	37.0	40.0	33.0	40.0
44-45	37.721810787551476	40.0	37.0	40.0	33.0	40.0
46-47	37.68012440067206	40.0	37.0	40.0	33.0	40.0
48-49	37.66776289774714	40.0	37.0	40.0	33.0	40.0
50-51	37.58299165731019	40.0	37.0	40.0	33.0	40.0
52-53	37.487129859807396	40.0	37.0	40.0	33.0	40.0
54-55	37.30645912062822	40.0	37.0	40.0	33.0	40.0
56-57	37.18982766606311	38.5	37.0	40.0	33.0	40.0
58-59	37.07838769783157	37.0	37.0	40.0	33.0	40.0
60-61	36.94278080966011	37.0	37.0	40.0	33.0	40.0
62-63	36.7906170381345	37.0	37.0	40.0	33.0	40.0
64-65	36.60884989422675	37.0	37.0	40.0	33.0	40.0
66-67	36.40725586304084	37.0	37.0	40.0	33.0	40.0
68-69	36.196174991094324	37.0	37.0	40.0	33.0	40.0
70-71	35.94290760574292	37.0	37.0	38.5	33.0	40.0
72-73	35.748805152908844	37.0	33.0	37.0	33.0	40.0
74-75	35.55307194238342	37.0	33.0	37.0	33.0	40.0
76-77	33.73392038764818	35.0	33.0	37.0	27.0	37.0
78-79	35.0274173591056	37.0	33.0	37.0	33.0	37.0
80-81	35.083176950167214	37.0	33.0	37.0	33.0	37.0
82-83	34.94593304495095	37.0	33.0	37.0	33.0	37.0
84-85	34.80262468399644	37.0	33.0	37.0	33.0	37.0
86-87	34.699640720242755	37.0	33.0	37.0	33.0	37.0
88-89	34.57922980838015	37.0	33.0	37.0	33.0	37.0
90-91	34.51932806547653	37.0	33.0	37.0	33.0	37.0
92-93	34.46069317083291	37.0	33.0	37.0	33.0	37.0
94-95	34.476625377887046	37.0	33.0	37.0	33.0	37.0
96-97	34.461606759251126	37.0	33.0	37.0	33.0	37.0
98-99	34.45799555940896	37.0	33.0	37.0	33.0	37.0
100	34.44161575239022	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	0.0
16	0.0
17	0.0
18	5.0
19	20.0
20	108.0
21	476.0
22	1825.0
23	5390.0
24	12233.0
25	24361.0
26	41353.0
27	65007.0
28	94760.0
29	130939.0
30	175352.0
31	232277.0
32	303409.0
33	403241.0
34	546271.0
35	780645.0
36	1329452.0
37	3068946.0
38	3596417.0
39	101271.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.31236164255032	20.481978713110788	14.476394936300597	23.729264708038293
2	14.370226209848852	21.236988902083244	41.92552337599507	22.467261512072835
3	17.67816957675448	26.66112320593453	31.0051256395596	24.65558157775139
4	12.133429005931951	18.55079286787716	39.29816433775481	30.01761378843607
5	13.052192828136224	37.870110759261706	35.88673381126211	13.190962601339958
6	29.045899634653434	38.52189353482356	18.98173010607631	13.4504767244467
7	25.631074899942824	33.09838222574072	23.496815029833897	17.77372784448256
8	23.956647388251163	36.84532186890677	21.550079899136502	17.64795084370556
9	25.03217956048145	16.997890736098284	21.485638313468503	36.484291389951764
10-11	23.615216503976356	27.005114803957714	29.228250995812775	20.15141769625315
12-13	24.66745875550303	24.56125336833991	29.297229304770244	21.474058571386813
14-15	21.89541603305379	25.945297263855277	26.8936549282895	25.265631774801435
16-17	21.414640783744556	29.186068779229156	27.507669217574875	21.89162121945141
18-19	21.388504053598393	28.031301769509316	29.423580874052575	21.15661330283972
20-21	22.057007370527383	27.165755590387143	29.454121174702884	21.32311586438259
22-23	21.745479520845702	27.264344938309616	29.32856268373324	21.66161285711144
24-25	21.632424972244703	27.457215869184708	29.388794988767273	21.521564169803316
26-27	21.514627573438588	27.55830627571055	29.350075663853993	21.57699048699687
28-29	21.398524021794785	27.3704056610229	29.428237310790312	21.802833006392003
30-31	21.616968960204495	27.260631997634782	29.44462399850154	21.67777504365918
32-33	21.470355632336954	27.508143511230752	29.35025477733956	21.671246079092732
34-35	21.55987125969141	27.726873723473865	29.391916031328936	21.32133898550579
36-37	21.664791764696034	27.57184537856669	29.05441249055206	21.708950366185213
38-39	21.61858153723186	27.569728989592047	29.173283662540783	21.638405810635305
40-41	21.746514281285087	27.491484879634985	29.112963644772687	21.649037194307237
42-43	21.8301658080843	27.517571133599223	29.002772422073882	21.649490636242593
44-45	21.74515858488214	27.745430752404133	28.73706370597848	21.77234695673525
46-47	22.01584098538999	27.572505154496586	28.343073181689626	22.0685806784238
48-49	21.887553598960785	27.718360928274627	28.34801500554559	22.046070467218996
50-51	21.80466624402964	27.955894592820734	28.240983300833282	21.998455862316344
52-53	21.94632313394408	27.908056598775527	28.26145411663902	21.88416615064137
54-55	21.949170932857967	27.854322513709928	28.105130061614396	22.09137649181771
56-57	22.094523059226212	27.96169628343172	27.9210274977359	22.02275315960617
58-59	21.83258394993874	28.079051628477764	28.191333283722784	21.897031137860708
60-61	22.061460743773303	28.226906180891014	27.9019659514671	21.80966712386858
62-63	21.903706883406763	28.366375059208266	27.876507783047945	21.85341027433703
64-65	21.95940135766008	28.165737820862574	27.866300018712302	22.008560802765043
66-67	22.02470471275859	28.099705483912185	27.770245933669628	22.105343869659595
68-69	21.97520680991018	28.019273389762596	27.86705107015259	22.13846873017463
70-71	21.979860692125026	27.839071504078245	27.933029106217056	22.248038697579673
72-73	22.07944441634474	27.85771377372613	27.824098612124708	22.238743197804425
74-75	21.978519817877114	27.943288751101765	27.830831611381406	22.24735981963972
76-77	22.09570264753221	27.902241218495295	27.746905707752155	22.25515042622034
78-79	22.06685576858732	27.851626463161317	27.723978569662933	22.357539198588427
80-81	22.074319327853537	27.898978231494816	27.714053619251455	22.31264882140019
82-83	22.13323671862403	27.868539669821534	27.782090624589888	22.216132986964542
84-85	22.19477272600473	27.806627707380095	27.746496422808477	22.2521031438067
86-87	22.118733445498872	27.779766084030268	27.832812271757824	22.268688198713036
88-89	22.13904720509999	27.73283051323275	27.83107527371395	22.29704700795331
90-91	22.14358076644375	27.757406673202496	27.83164419707686	22.26736836327689
92-93	22.196309667647075	27.60275771713465	27.921264528980544	22.279668086237727
94-95	22.050340348694185	27.706899246383195	27.920750164248894	22.32201024067373
96-97	22.159552014312016	27.82256409313838	27.966719662785387	22.051164229764215
98-99	23.27296420590423	29.701204171990103	28.17692178827133	18.848909833834334
100	22.17282929255125	30.71337237600158	22.057544069839768	25.056254261607403
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2910.0
1	2461.5
2	3319.5
3	4914.0
4	6559.5
5	10379.5
6	13869.5
7	17053.5
8	19784.0
9	19907.5
10	17471.5
11	15109.0
12	16216.0
13	21417.0
14	33367.0
15	49248.5
16	61757.5
17	68799.5
18	69993.5
19	66067.0
20	60133.0
21	54972.5
22	53173.0
23	57062.5
24	66625.0
25	82428.0
26	103093.5
27	125660.5
28	149687.5
29	175755.0
30	198604.5
31	218887.5
32	240249.5
33	261252.0
34	283708.0
35	304424.0
36	325562.5
37	344956.5
38	360623.5
39	376108.0
40	390231.5
41	400928.5
42	407678.0
43	415960.5
44	422458.0
45	430109.0
46	441914.5
47	451308.5
48	451906.0
49	445868.5
50	440638.0
51	427088.0
52	404516.0
53	376492.5
54	346640.0
55	318861.5
56	290425.0
57	260910.0
58	230141.5
59	199268.0
60	166750.5
61	134751.5
62	105982.0
63	80139.5
64	58311.5
65	42149.0
66	31248.5
67	23652.5
68	17712.0
69	12698.0
70	9055.0
71	6555.0
72	4594.5
73	3121.5
74	2140.0
75	1460.0
76	965.5
77	634.0
78	410.5
79	265.0
80	185.0
81	128.0
82	96.0
83	63.0
84	42.0
85	34.5
86	30.0
87	25.5
88	20.5
89	17.0
90	13.5
91	8.5
92	4.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.001383574496782044
5	0.0
6	1.1911568515342102E-4
7	0.0
8	0.0
9	0.0
10-11	0.004017863687675008
12-13	9.162745011801616E-6
14-15	0.001429388221841052
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	162.0
20-21	709.0
22-23	1520.0
24-25	3257.0
26-27	4199.0
28-29	8653.0
30-31	18117.0
32-33	23328.0
34-35	33349.0
36-37	45733.0
38-39	52699.0
40-41	58156.0
42-43	72325.0
44-45	86562.0
46-47	84947.0
48-49	77688.0
50-51	67537.0
52-53	66005.0
54-55	75386.0
56-57	81949.0
58-59	82238.0
60-61	84492.0
62-63	87863.0
64-65	93798.0
66-67	97657.0
68-69	87232.0
70-71	86370.0
72-73	83559.0
74-75	86853.0
76-77	85616.0
78-79	92953.0
80-81	94969.0
82-83	92293.0
84-85	94921.0
86-87	100227.0
88-89	108736.0
90-91	114397.0
92-93	121241.0
94-95	137974.0
96-97	366312.0
98-99	666989.0
100-101	7184789.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.50289453275639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.89040042454982	72.51212436668361
2	8.186362098442974	13.507971376295899
3	2.1620063899695827	5.351153555124176
4	0.8164085190066556	2.6942426375699977
5	0.4000170841225326	1.6501283651331022
6	0.18513410100079838	0.916445952557132
7	0.1053199406439294	0.6082439968599589
8	0.06839846853861087	0.4514457308834454
9	0.043042235521690855	0.31959981159301093
>10	0.13957984970051227	1.7358773908072822
>50	0.002723247179919844	0.1511326884694885
>100	5.68693452187942E-4	0.07902900846527815
>500	3.894787078650636E-5	0.022605119557629956
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.162745011801616E-6
2	0.0	3.6650980047206465E-5	0.0	0.0	1.8325490023603232E-5
3	0.0	3.6650980047206465E-5	0.0	0.0	1.8325490023603232E-5
4	0.0	6.413921508261131E-5	0.0	0.0	1.8325490023603232E-5
5	0.0	9.162745011801615E-5	0.0	9.162745011801616E-6	1.8325490023603232E-5
6	0.0	9.162745011801615E-5	0.0	9.162745011801616E-6	1.8325490023603232E-5
7	0.0	1.0079019512981777E-4	0.0	9.162745011801616E-6	1.8325490023603232E-5
8	0.0	1.19115685153421E-4	0.0	9.162745011801616E-6	1.8325490023603232E-5
9	0.0	2.5655686033044525E-4	0.0	1.8325490023603232E-5	1.8325490023603232E-5
10-11	0.0	2.5655686033044525E-4	0.0	2.2906862529504038E-5	1.8325490023603232E-5
12-13	0.0	2.611382328363461E-4	0.0	2.7488235035404847E-5	2.2906862529504038E-5
14-15	0.0	3.848352904956678E-4	0.0	3.6650980047206465E-5	3.665098004720646E-5
16-17	0.0	4.123235255310727E-4	0.0	5.0395097564908885E-5	6.413921508261131E-5
18-19	0.0	4.260676430487751E-4	0.0	1.0537156763571859E-4	6.413921508261131E-5
20-21	0.0	4.443931330723784E-4	0.0	1.6951078271832987E-4	6.413921508261131E-5
22-23	0.0	4.947882306372872E-4	0.0	3.023705853894533E-4	6.872058758851211E-5
24-25	0.0	5.589274457198986E-4	0.0	8.017401885326413E-4	7.330196009441293E-5
26-27	0.0	5.864156807553034E-4	0.0	0.002171570567796983	7.330196009441293E-5
28-29	0.0	5.864156807553034E-4	0.0	0.008695445016199732	7.330196009441293E-5
30-31	0.0	6.597176408497163E-4	0.0	0.010280599903241414	7.330196009441293E-5
32-33	9.162745011801616E-6	6.597176408497163E-4	0.0	0.013253910659571037	7.330196009441293E-5
34-35	9.162745011801616E-6	7.87996071014939E-4	0.0	0.016987729251880194	8.246470510621453E-5
36-37	9.162745011801616E-6	9.254372461919632E-4	0.0	0.02258616645409098	8.246470510621453E-5
38-39	9.162745011801616E-6	9.758323437568721E-4	0.0	0.03659142220462975	8.246470510621453E-5
40-41	9.162745011801616E-6	0.0010812039113925907	0.0	0.05173743970913783	8.246470510621453E-5
42-43	9.162745011801616E-6	0.0011041107739220945	0.0	0.06162862294937767	8.246470510621453E-5
44-45	9.162745011801616E-6	0.001214063714063714	0.0	0.07411744440046328	8.246470510621453E-5
46-47	9.162745011801616E-6	0.0012736215566404245	0.0	0.08695445016199733	9.162745011801615E-5
48-49	9.162745011801616E-6	0.0012827843016522261	0.0	0.09854074122942047	1.0079019512981777E-4
50-51	9.162745011801616E-6	0.0014202254768292503	0.0	0.11031028719707965	1.0079019512981777E-4
52-53	9.162745011801616E-6	0.001429388221841052	0.0	0.1366302722434798	1.0995294014161939E-4
54-55	9.162745011801616E-6	0.0014889460644177625	0.0	0.1690022503701749	1.19115685153421E-4
56-57	9.162745011801616E-6	0.0015210156719590682	0.0	0.2035412176921611	1.19115685153421E-4
58-59	9.162745011801616E-6	0.0015347597894767705	0.0	0.23496943308264062	1.3285980267112343E-4
60-61	9.162745011801616E-6	0.0015622480245121753	0.0	0.2473528829660905	1.4660392018882586E-4
62-63	9.162745011801616E-6	0.001598899004559382	0.0	0.2619445543973846	1.4660392018882586E-4
64-65	9.162745011801616E-6	0.00181422351233672	0.0	0.26799654747767954	1.4660392018882586E-4
66-67	9.162745011801616E-6	0.0018233862573485216	0.0	0.27297649939159374	1.4660392018882586E-4
68-69	9.162745011801616E-6	0.0018462931198780256	0.0	0.27812138071572035	1.4660392018882586E-4
70-71	9.162745011801616E-6	0.001864618609901629	0.0	0.2828401943967982	1.649294102124291E-4
72-73	9.162745011801616E-6	0.001878362727419331	0.0	0.28669771004676664	1.9241764524783393E-4
74-75	9.162745011801616E-6	0.0019150137074665377	0.0	0.2896893462931199	1.9241764524783393E-4
76-77	9.162745011801616E-6	0.0019974784125727525	0.0	0.2923877746990955	1.9241764524783393E-4
78-79	9.162745011801616E-6	0.0020341293926199587	0.0	0.2944173227192095	1.9241764524783393E-4
80-81	9.162745011801616E-6	0.00204329213763176	0.0	0.29519157467270674	1.9699901775373474E-4
82-83	9.162745011801616E-6	0.0020570362551494627	0.0	0.29568178153083813	2.0158039025963554E-4
84-85	9.162745011801616E-6	0.0020707803726671653	0.0	0.29587878054859185	2.0158039025963554E-4
86-87	9.162745011801616E-6	0.0021349195877497762	0.0	0.2959704079987099	2.0158039025963554E-4
88	9.162745011801616E-6	0.0022265470378677924	0.0	0.2959841521162276	2.0158039025963554E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15480	0.0	48.207615	1
GTATCAA	27005	0.0	36.63876	1
TCAACGC	33165	0.0	29.356152	4
ATCAACG	33500	0.0	29.061523	3
CAACGCA	33965	0.0	28.678648	5
TATCAAC	34600	0.0	28.298742	2
AACGCAG	34480	0.0	28.225418	6
CTTATAC	11015	0.0	27.333166	1
ACGCAGA	39475	0.0	24.686377	7
CGCAGAG	40430	0.0	24.114088	8
TATACAC	15985	0.0	21.330048	3
GCAGAGT	47110	0.0	20.467571	9
GAGTACT	28885	0.0	20.193419	12-13
GTGGTAT	7385	0.0	20.151917	1
GTACATG	31470	0.0	20.006296	1
TTATACA	16585	0.0	19.420563	2
TACATGG	31045	0.0	19.340769	2
AGAGTAC	42070	0.0	18.983765	10-11
CAGAGTA	45060	0.0	18.823503	10-11
ACATGGG	31150	0.0	18.642235	3
>>END_MODULE