FastQCFastQC Report
Fri 27 May 2016
SRR2139220_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139220_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12298676
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA160050.051.092871
GTATCAA289950.038.277421
CTTATAC83250.032.273161
TCAACGC357300.030.6011164
ATCAACG358500.030.5224063
CAACGCA367350.029.753325
TATCAAC371400.029.6843032
AACGCAG374450.029.2123476
ACGCAGA426850.025.5754017
CGCAGAG446550.024.4569388
TATACAC131400.024.1136263
GTACATG363700.021.7201651
GCAGAGT509450.021.2841329
TTATACA135450.021.2456282
TACATGG364250.020.9962332
GAGTACT308050.020.4235912-13
ACATGGG364750.020.3010623
GTGGTAT79050.019.8765831
TGGTATC75450.019.1566722
AGAGTAC455350.019.15120710-11
Produced by FastQC (version 0.11.5)