##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139220_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12298676
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51361268481258	33.0	33.0	33.0	33.0	33.0
2	32.540142532415686	33.0	33.0	33.0	33.0	33.0
3	32.53386600313725	33.0	33.0	33.0	33.0	33.0
4	36.43996784694548	37.0	37.0	37.0	37.0	37.0
5	36.476128649945736	37.0	37.0	37.0	37.0	37.0
6	36.474555309856115	37.0	37.0	37.0	37.0	37.0
7	36.45416742420078	37.0	37.0	37.0	37.0	37.0
8	36.4473380711875	37.0	37.0	37.0	37.0	37.0
9	36.47226644559138	37.0	37.0	37.0	37.0	37.0
10-11	36.422454782937606	37.0	37.0	37.0	37.0	37.0
12-13	36.40350323075427	37.0	37.0	37.0	37.0	37.0
14-15	38.352240111049355	40.0	37.0	40.0	37.0	40.0
16-17	38.31875036792578	40.0	37.0	40.0	37.0	40.0
18-19	38.34207035781738	40.0	37.0	40.0	37.0	40.0
20-21	38.33515410898124	40.0	37.0	40.0	37.0	40.0
22-23	38.33432348143414	40.0	37.0	40.0	37.0	40.0
24-25	38.307597247752994	40.0	37.0	40.0	37.0	40.0
26-27	38.18352154331951	40.0	37.0	40.0	37.0	40.0
28-29	38.12356979404248	40.0	37.0	40.0	33.0	40.0
30-31	38.04555311964484	40.0	37.0	40.0	33.0	40.0
32-33	37.968993300823136	40.0	37.0	40.0	33.0	40.0
34-35	37.94178943007746	40.0	37.0	40.0	33.0	40.0
36-37	37.88337667717035	40.0	37.0	40.0	33.0	40.0
38-39	37.82771067988753	40.0	37.0	40.0	33.0	40.0
40-41	37.79933982538782	40.0	37.0	40.0	33.0	40.0
42-43	37.73887817778903	40.0	37.0	40.0	33.0	40.0
44-45	37.665478026582846	40.0	37.0	40.0	33.0	40.0
46-47	37.62112422710389	40.0	37.0	40.0	33.0	40.0
48-49	37.59895625865025	40.0	37.0	40.0	33.0	40.0
50-51	37.510845015595294	40.0	37.0	40.0	33.0	40.0
52-53	37.40342121535632	40.0	37.0	40.0	33.0	40.0
54-55	37.21565773798267	40.0	37.0	40.0	33.0	40.0
56-57	37.09870539518809	37.0	37.0	40.0	33.0	40.0
58-59	36.97376525478616	37.0	37.0	40.0	33.0	40.0
60-61	36.83324606308506	37.0	37.0	40.0	33.0	40.0
62-63	36.669578301209285	37.0	37.0	40.0	33.0	40.0
64-65	36.47932458102788	37.0	37.0	40.0	33.0	40.0
66-67	36.270607838055675	37.0	37.0	40.0	33.0	40.0
68-69	36.056376539080745	37.0	37.0	40.0	33.0	40.0
70-71	35.7967546308616	37.0	35.0	37.0	33.0	40.0
72-73	35.59792329616868	37.0	33.0	37.0	33.0	40.0
74-75	35.40611398462605	37.0	33.0	37.0	33.0	40.0
76-77	33.58327758635136	35.0	33.0	37.0	27.0	37.0
78-79	34.897687233352336	37.0	33.0	37.0	33.0	37.0
80-81	34.958577093781145	37.0	33.0	37.0	33.0	37.0
82-83	34.826266127224336	37.0	33.0	37.0	33.0	37.0
84-85	34.681142885239495	37.0	33.0	37.0	33.0	37.0
86-87	34.58093460130628	37.0	33.0	37.0	33.0	37.0
88-89	34.46037496866484	37.0	33.0	37.0	30.0	37.0
90-91	34.397077282030835	37.0	33.0	37.0	30.0	37.0
92-93	34.32923949463525	37.0	33.0	37.0	27.0	37.0
94-95	34.34822544028539	37.0	33.0	37.0	33.0	37.0
96-97	34.334462518557515	37.0	33.0	37.0	33.0	37.0
98-99	34.33374709571753	37.0	33.0	37.0	33.0	37.0
100	34.3144494998537	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	2.0
19	15.0
20	102.0
21	580.0
22	2308.0
23	6460.0
24	14579.0
25	28279.0
26	49080.0
27	77211.0
28	112294.0
29	156338.0
30	208512.0
31	275229.0
32	359925.0
33	476262.0
34	644942.0
35	921547.0
36	1571520.0
37	3532561.0
38	3776688.0
39	84241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.303551699386176	20.488270444721042	14.420771796899112	23.787406058993668
2	14.647991377283212	21.123306281098873	41.66827388574185	22.560428455876064
3	18.014776549931067	26.375782238673494	30.632069663433693	24.97737154796175
4	12.404984471297187	18.366630575591397	38.690546078892766	30.537838874218647
5	13.420151892772846	37.76082075826699	35.52650708092481	13.292520268035357
6	29.82025113469688	37.88605733356783	18.787999569709186	13.505691962026104
7	26.25847692873607	32.73281611776747	23.14452384955909	17.864183103937368
8	24.358947255785907	36.78917958323319	21.102637389585677	17.74923577139523
9	25.4277208375926	16.873369133392895	21.169742173873026	36.52916785514148
10-11	24.131768915787934	26.7803020385316	28.817272429076013	20.27065661660446
12-13	25.0312381224497	24.327154117299386	28.884222442020075	21.75738531823084
14-15	22.161329935355898	25.67674859337435	26.508935952922684	25.652985518347066
16-17	21.73900670283533	28.877205969162862	27.141669558576876	22.242117769424937
18-19	21.76324101878934	27.87389878390162	28.94496122997305	21.417898967335997
20-21	22.543611601095094	27.023924314119885	28.886880301264288	21.545583783520726
22-23	22.3056758360288	27.11106868043936	28.68914948332887	21.894106000202974
24-25	22.10703559787637	27.296895682992446	28.83197810487618	21.76409061425501
26-27	22.080648520586816	27.40441659832598	28.819060706056938	21.695874175030266
28-29	22.00308856204422	27.292083184022992	28.89968603621175	21.805142217721038
30-31	22.29268530326633	27.19019801733379	28.729951300962785	21.78716537843709
32-33	22.157535069459275	27.18205832670722	28.73563513802331	21.924771465810203
34-35	22.0855333019982	27.393004074098553	28.81759580892404	21.703866814979207
36-37	22.27855055077967	27.27935949311145	28.568133541929917	21.873956414178966
38-39	22.033619511664483	27.369469236175686	28.667596462016103	21.92931479014373
40-41	22.169873242534468	27.248150788200793	28.63733482934378	21.944641139920957
42-43	22.217757224949665	27.286607316804595	28.431438984192916	22.06419647405283
44-45	22.08469096203152	27.48282112684383	28.256404696293313	22.176083214831333
46-47	22.36188245359787	27.375574836212994	27.881766085703873	22.380776624485254
48-49	22.324596628297765	27.533907423510335	27.825194947242693	22.31630100094921
50-51	22.273354091085633	27.648731875471217	27.812390134350238	22.26552389909291
52-53	22.40842225373906	27.629082805651016	27.80344640252293	22.159048538086985
54-55	22.404369865365954	27.59282425302346	27.61792633366872	22.38487954794187
56-57	22.47053699480796	27.827985795161325	27.428872224436557	22.272604985594157
58-59	22.25162910736475	27.885319088729027	27.707047491944774	22.156004311961446
60-61	22.47982125588224	27.95695670473437	27.4483443153937	22.11487772398969
62-63	22.328253254665857	28.08794822131967	27.487286215731444	22.096512308283028
64-65	22.43225941529062	27.889192800348923	27.403146264842842	22.27540151951762
66-67	22.46621483238965	27.832068710498824	27.341892887638746	22.359823569472784
68-69	22.4043716590837	27.77586902845809	27.434703530998693	22.385055781459517
70-71	22.446909835312233	27.63992652905573	27.451873285026384	22.46129035060565
72-73	22.48060233362632	27.688894298693516	27.377424080574066	22.453079287106103
74-75	22.355288568709337	27.781371307748536	27.3618056650971	22.50153445844503
76-77	22.463440797125514	27.765165052028777	27.296780166225965	22.474613984619744
78-79	22.37927703963261	27.72741269413285	27.328597824343465	22.564712441891082
80-81	22.371830835022525	27.773021287936295	27.356383224431998	22.498764652609182
82-83	22.478082590689546	27.670535762840753	27.44384639571874	22.40753525075096
84-85	22.510609257188307	27.669443499010214	27.368077056516388	22.451870187285085
86-87	22.421198791710324	27.74040591943644	27.379497937504567	22.458897351348668
88-89	22.48437388573622	27.681971807113865	27.33186210182571	22.501792205324207
90-91	22.481586989599073	27.7515352019228	27.337457498786716	22.429420309691412
92-93	22.5128815323494	27.725188114815335	27.396380391358456	22.36554996147681
94-95	22.30447901443956	27.82541490812649	27.425356885069135	22.444749192364817
96-97	22.415707942002097	27.94300115727093	27.45642121467462	22.184869686052348
98-99	23.537361248371923	29.828187694016865	27.65872430217308	18.97572675543813
100	22.42368346361677	30.870822808478906	21.52242738398998	25.183066343914344
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4097.0
1	3339.0
2	3795.5
3	5253.5
4	6862.0
5	10825.5
6	14300.0
7	17093.5
8	19172.5
9	19050.0
10	17305.5
11	15442.5
12	16572.5
13	21624.0
14	33552.0
15	50351.0
16	64243.5
17	72164.5
18	73522.5
19	69272.5
20	62179.5
21	55177.5
22	51533.5
23	53304.5
24	60618.5
25	74186.0
26	92434.0
27	112557.5
28	135302.5
29	161267.0
30	185785.5
31	210648.5
32	239198.0
33	267167.0
34	294456.0
35	320793.0
36	347587.5
37	372919.5
38	393792.5
39	411832.0
40	428697.5
41	442505.5
42	450579.0
43	460635.0
44	471264.5
45	482420.0
46	497292.0
47	512842.0
48	518813.0
49	512150.5
50	504437.5
51	493870.0
52	469259.5
53	434808.5
54	404153.5
55	375400.5
56	344143.0
57	310159.5
58	274789.0
59	236777.5
60	196469.0
61	162794.5
62	132059.5
63	103338.0
64	79876.0
65	58763.0
66	44081.5
67	33419.0
68	23657.0
69	16542.0
70	11842.5
71	8663.5
72	6151.0
73	4388.5
74	3297.0
75	2370.5
76	1554.0
77	930.5
78	625.0
79	455.0
80	323.5
81	220.0
82	169.0
83	114.5
84	70.0
85	49.5
86	34.5
87	23.0
88	14.5
89	9.5
90	8.0
91	8.0
92	7.5
93	5.0
94	2.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0013009530456774372
5	0.0
6	1.382262611032277E-4
7	0.0
8	0.0
9	0.0
10-11	0.004146787833096831
12-13	1.2196434803225974E-5
14-15	0.0014920305242613109
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	200.0
20-21	911.0
22-23	1784.0
24-25	3318.0
26-27	4980.0
28-29	8461.0
30-31	17555.0
32-33	22203.0
34-35	31241.0
36-37	42598.0
38-39	51462.0
40-41	55674.0
42-43	70683.0
44-45	84616.0
46-47	82715.0
48-49	73504.0
50-51	64654.0
52-53	64055.0
54-55	73192.0
56-57	79036.0
58-59	79174.0
60-61	81733.0
62-63	83136.0
64-65	89342.0
66-67	92054.0
68-69	84168.0
70-71	83447.0
72-73	81047.0
74-75	85389.0
76-77	82595.0
78-79	90239.0
80-81	93189.0
82-83	92533.0
84-85	94566.0
86-87	101635.0
88-89	109627.0
90-91	116849.0
92-93	124466.0
94-95	156003.0
96-97	427168.0
98-99	786241.0
100-101	8431233.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.36127826752558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.60910975754823	72.1559826751162
2	8.47183434443785	13.955022117572518
3	2.177728006292098	5.380813869516215
4	0.8095817384886631	2.6671274737588764
5	0.37381303722117737	1.539385978930114
6	0.19811863587461467	0.9790382459551031
7	0.11060179331283389	0.6376513553147963
8	0.061965982394487557	0.40828780152903854
9	0.043980450721107255	0.32600575261550796
>10	0.14017744681665736	1.7258018292289523
>50	0.0025939524843995388	0.14550254477751776
>100	4.6024550709207706E-4	0.059968993960525234
>500	3.4608900827137316E-5	0.019411361724683843
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.130956535483982E-6
2	0.0	1.6261913070967964E-5	0.0	0.0	8.130956535483982E-6
3	0.0	1.6261913070967964E-5	0.0	0.0	8.130956535483982E-6
4	0.0	4.065478267741991E-5	0.0	0.0	8.130956535483982E-6
5	0.0	6.504765228387186E-5	0.0	0.0	8.130956535483982E-6
6	4.065478267741991E-5	6.504765228387186E-5	0.0	0.0	8.130956535483982E-6
7	4.065478267741991E-5	6.504765228387186E-5	0.0	8.130956535483982E-6	8.130956535483982E-6
8	4.065478267741991E-5	7.317860881935583E-5	0.0	8.130956535483982E-6	8.130956535483982E-6
9	4.065478267741991E-5	1.382262611032277E-4	0.0	1.6261913070967964E-5	8.130956535483982E-6
10-11	4.065478267741991E-5	1.382262611032277E-4	0.0	1.6261913070967964E-5	8.130956535483982E-6
12-13	4.065478267741991E-5	1.4635721763871167E-4	0.0	2.0327391338709956E-5	1.6261913070967964E-5
14-15	4.065478267741991E-5	2.3173226126129348E-4	0.0	4.065478267741991E-5	1.6261913070967964E-5
16-17	6.911313055161385E-5	2.4392869606451947E-4	0.0	4.8785739212903896E-5	1.6261913070967964E-5
18-19	7.317860881935583E-5	2.561251308677454E-4	0.0	6.098217401612987E-5	2.4392869606451948E-5
20-21	7.317860881935583E-5	2.7238704393871336E-4	0.0	7.317860881935585E-5	2.4392869606451948E-5
22-23	7.317860881935583E-5	3.333692179548433E-4	0.0	8.130956535483982E-5	4.065478267741991E-5
24-25	7.317860881935583E-5	3.6182756582903717E-4	0.0	1.0163695669354978E-4	5.2851217480645884E-5
26-27	7.317860881935583E-5	3.658930440967792E-4	0.0	1.341607828354857E-4	6.098217401612987E-5
28-29	7.317860881935583E-5	3.699585223645212E-4	0.0	2.3173226126129348E-4	6.504765228387186E-5
30-31	7.317860881935583E-5	4.268752181129091E-4	0.0	6.342146097677506E-4	6.504765228387186E-5
32-33	7.317860881935583E-5	4.3907165291613503E-4	0.0	0.0016302567853645387	8.130956535483982E-5
34-35	7.724408708709784E-5	5.041193052000069E-4	0.0	0.0028946205266322978	8.94405218903238E-5
36-37	8.537504362258181E-5	5.732324357516207E-4	0.0	0.0046143178338871604	8.94405218903238E-5
38-39	8.94405218903238E-5	6.016907836258147E-4	0.0	0.007569920534535588	8.94405218903238E-5
40-41	8.94405218903238E-5	6.667384359096865E-4	0.0	0.010492999409042079	9.757147842580779E-5
42-43	8.94405218903238E-5	6.951967837838804E-4	0.0	0.013078643587325984	1.0570243496129177E-4
44-45	1.0976791322903377E-4	7.846373056742043E-4	0.0	0.016461121506087324	1.1789886976451775E-4
46-47	1.2196434803225974E-4	8.618813927613021E-4	0.0	0.020063135251306728	1.300953045677437E-4
48-49	1.2196434803225974E-4	8.70012349296786E-4	0.0	0.023657018039990645	1.300953045677437E-4
50-51	1.300953045677437E-4	0.0010082386104000138	0.0	0.026860614914971336	1.300953045677437E-4
52-53	1.300953045677437E-4	0.0010123040886677558	0.0	0.030344729790426222	1.6261913070967965E-4
54-55	1.300953045677437E-4	0.0010692207844161437	0.0	0.03388982683989723	1.788810437806476E-4
56-57	1.341607828354857E-4	0.0011220720018967896	0.0	0.03829273980386182	1.788810437806476E-4
58-59	1.382262611032277E-4	0.0011220720018967896	0.0	0.04257368841979413	1.788810437806476E-4
60-61	1.382262611032277E-4	0.0011220720018967896	0.0	0.046484678513361924	1.788810437806476E-4
62-63	1.5448817417419567E-4	0.0011383339149677574	0.0	0.050468847215749076	1.788810437806476E-4
64-65	1.5448817417419567E-4	0.0012521673064645332	0.0	0.054497736179081394	1.829465220483896E-4
66-67	1.6261913070967965E-4	0.0012887566108742113	0.0	0.058827470534226614	1.9514295685161558E-4
68-69	1.6261913070967965E-4	0.001317214958748405	0.0	0.06325884184606538	1.9514295685161558E-4
70-71	1.7075008724516363E-4	0.0013294113935516311	0.0	0.06732025463553963	1.9514295685161558E-4
72-73	1.8701200031613158E-4	0.001337542350087115	0.0	0.0703571669015429	2.1140486992258354E-4
74-75	1.8701200031613158E-4	0.0013538042631580831	0.0	0.07301598968864616	2.1140486992258354E-4
76-77	1.910774785838736E-4	0.0014025900023709869	0.0	0.07547560404063006	2.1140486992258354E-4
78-79	1.9514295685161558E-4	0.0014351138285129228	0.0	0.07739450978300427	2.1140486992258354E-4
80-81	2.0327391338709956E-4	0.0014473102633161487	0.0	0.0780246589145043	2.1140486992258354E-4
82-83	2.1140486992258354E-4	0.0014554412198516328	0.0	0.07847186152395591	2.236013047258095E-4
84-85	2.1547034819032553E-4	0.0014635721763871167	0.0	0.07865074256773656	2.276667829935515E-4
86-87	2.1953582645806751E-4	0.0015042269590645368	0.0	0.07869952830694946	2.276667829935515E-4
88	2.1953582645806751E-4	0.0015855365244193764	0.0	0.07870765926348494	2.276667829935515E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16005	0.0	51.09287	1
GTATCAA	28995	0.0	38.27742	1
CTTATAC	8325	0.0	32.27316	1
TCAACGC	35730	0.0	30.601116	4
ATCAACG	35850	0.0	30.522406	3
CAACGCA	36735	0.0	29.75332	5
TATCAAC	37140	0.0	29.684303	2
AACGCAG	37445	0.0	29.212347	6
ACGCAGA	42685	0.0	25.575401	7
CGCAGAG	44655	0.0	24.456938	8
TATACAC	13140	0.0	24.113626	3
GTACATG	36370	0.0	21.720165	1
GCAGAGT	50945	0.0	21.284132	9
TTATACA	13545	0.0	21.245628	2
TACATGG	36425	0.0	20.996233	2
GAGTACT	30805	0.0	20.42359	12-13
ACATGGG	36475	0.0	20.301062	3
GTGGTAT	7905	0.0	19.876583	1
TGGTATC	7545	0.0	19.156672	2
AGAGTAC	45535	0.0	19.151207	10-11
>>END_MODULE