FastQCFastQC Report
Fri 27 May 2016
SRR2139219_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139219_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9161365
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124900.046.499861
GTATCAA222150.036.7827381
ATCAACG254650.031.4143583
TCAACGC264150.030.3667434
CAACGCA269700.029.679365
AACGCAG270500.029.6707156
TATCAAC275650.029.207832
ACGCAGA275850.029.1108137
CGCAGAG320150.025.0560118
GTACATG272650.021.7733861
GCAGAGT376550.021.1325669
TACATGG272150.021.056742
ACATGGG267050.020.7521443
GAGTACT220200.019.72311412-13
CAGAGTA366950.018.6047410-11
AGAGTAC337000.018.36793310-11
CATGGGG207900.017.9503144
GTGGTAT66050.017.9238661
GTACTTT250650.017.32829914-15
AGTACTT241300.016.6030312-13
Produced by FastQC (version 0.11.5)