##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139219_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9161365
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63396393441371	33.0	33.0	33.0	33.0	33.0
2	32.6740719314207	33.0	33.0	33.0	33.0	33.0
3	33.35444772694898	33.0	33.0	33.0	33.0	37.0
4	36.47076718371116	37.0	37.0	37.0	37.0	37.0
5	36.50291370336189	37.0	37.0	37.0	37.0	37.0
6	36.49320194097714	37.0	37.0	37.0	37.0	37.0
7	36.48440237890314	37.0	37.0	37.0	37.0	37.0
8	36.46853847652615	37.0	37.0	37.0	37.0	37.0
9	36.485343614188494	37.0	37.0	37.0	37.0	37.0
10-11	36.46014163828207	37.0	37.0	37.0	37.0	37.0
12-13	36.67323330093278	37.0	37.0	37.0	37.0	38.5
14-15	38.39393027130782	40.0	37.0	40.0	37.0	40.0
16-17	38.3647747906562	40.0	37.0	40.0	37.0	40.0
18-19	38.39661584272649	40.0	37.0	40.0	37.0	40.0
20-21	38.39241762658167	40.0	37.0	40.0	37.0	40.0
22-23	38.39602611832274	40.0	37.0	40.0	37.0	40.0
24-25	38.352925074952665	40.0	37.0	40.0	37.0	40.0
26-27	38.24271078553538	40.0	37.0	40.0	37.0	40.0
28-29	38.19009158585068	40.0	37.0	40.0	37.0	40.0
30-31	38.11439628367499	40.0	37.0	40.0	35.0	40.0
32-33	38.04628175481959	40.0	37.0	40.0	33.0	40.0
34-35	38.02649068957982	40.0	37.0	40.0	33.0	40.0
36-37	37.96840952882239	40.0	37.0	40.0	33.0	40.0
38-39	37.926943301762975	40.0	37.0	40.0	33.0	40.0
40-41	37.896224853900584	40.0	37.0	40.0	33.0	40.0
42-43	37.833266525320724	40.0	37.0	40.0	33.0	40.0
44-45	37.765896026744464	40.0	37.0	40.0	33.0	40.0
46-47	37.73082332722008	40.0	37.0	40.0	33.0	40.0
48-49	37.70709946125713	40.0	37.0	40.0	33.0	40.0
50-51	37.62886198937983	40.0	37.0	40.0	33.0	40.0
52-53	37.512838706933344	40.0	37.0	40.0	33.0	40.0
54-55	37.346394964662395	40.0	37.0	40.0	33.0	40.0
56-57	37.23348750707648	40.0	37.0	40.0	33.0	40.0
58-59	37.11800570875438	37.0	37.0	40.0	33.0	40.0
60-61	36.97992510539491	37.0	37.0	40.0	33.0	40.0
62-63	36.810633074901574	37.0	37.0	40.0	33.0	40.0
64-65	36.621009780292965	37.0	37.0	40.0	33.0	40.0
66-67	36.414773394617086	37.0	37.0	40.0	33.0	40.0
68-69	36.193718035149175	37.0	37.0	40.0	33.0	40.0
70-71	35.93748276688636	37.0	37.0	38.5	33.0	40.0
72-73	35.73413312827705	37.0	33.0	37.0	33.0	40.0
74-75	35.309992250508245	37.0	33.0	37.0	33.0	40.0
76-77	33.93686145816914	35.0	33.0	37.0	27.0	37.0
78-79	35.04924928294324	37.0	33.0	37.0	33.0	37.0
80-81	35.074090825848785	37.0	33.0	37.0	33.0	37.0
82-83	34.937023722030474	37.0	33.0	37.0	33.0	37.0
84-85	34.7973571775307	37.0	33.0	37.0	33.0	37.0
86-87	34.6978032386821	37.0	33.0	37.0	33.0	37.0
88-89	34.58446816473332	37.0	33.0	37.0	33.0	37.0
90-91	34.52907620707771	37.0	33.0	37.0	33.0	37.0
92-93	34.4702580350558	37.0	33.0	37.0	33.0	37.0
94-95	34.488607157465225	37.0	33.0	37.0	33.0	37.0
96-97	34.47205759920309	37.0	33.0	37.0	33.0	37.0
98-99	34.46155974762816	37.0	33.0	37.0	33.0	37.0
100	34.419057515590154	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	1.0
15	0.0
16	0.0
17	2.0
18	4.0
19	13.0
20	85.0
21	422.0
22	1531.0
23	4151.0
24	9639.0
25	18771.0
26	32706.0
27	51469.0
28	76099.0
29	106264.0
30	143616.0
31	190665.0
32	252409.0
33	336070.0
34	457235.0
35	652003.0
36	1100498.0
37	2537727.0
38	3088234.0
39	101749.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.4584273695489	6.549243103974473E-4	24.744022833281157	29.796894872859543
2	14.822466918950816	23.999813128243037	38.75170102963937	22.426018923166783
3	16.6969187814494	24.778637804522766	33.023269623241916	25.501173790785913
4	13.097545166753008	23.2216938386517	37.46763823459489	26.213122760000413
5	17.36941182737319	36.62506148105991	32.21406695382933	13.791459737737572
6	27.85864583161643	37.112526015523684	20.67860689197026	14.350221260889626
7	25.95875744689955	33.08631041036143	22.694516088926942	18.260416053812083
8	23.986367080673883	32.39428363973273	22.305840381236223	21.313508898357163
9	23.714913727958802	20.71194832268002	24.020055644874407	31.553082304486768
10-11	24.696852724056033	26.032790571571336	28.731557384318933	20.538799320053695
12-13	24.906322428984087	24.178825498811882	28.665072031730716	22.249780040473315
14-15	22.367135747538356	26.579083266921355	26.69739682875119	24.3563841567891
16-17	21.507084370069308	28.738086518766583	27.67954884452262	22.07528026664149
18-19	21.56639321760458	27.930395743429067	29.378296793108888	21.124914245857468
20-21	22.327290351313824	27.212767327726983	29.222097054482898	21.237845266476295
22-23	22.220027949143955	27.003963555853367	28.969835731913978	21.806172763088703
24-25	21.85511303331467	27.30845507014189	29.142691292691598	21.69374060385184
26-27	21.82202687424971	27.473591456501705	29.017454110610373	21.68692755863821
28-29	21.82935460282526	27.44602426874327	28.9649314927457	21.759689635685774
30-31	22.05318176805155	27.317161687139706	28.95258158143964	21.677074963369098
32-33	21.85237779446341	27.57332880263511	28.86741678952532	21.706876613376156
34-35	22.030337975616163	27.418525192217075	28.781928295763425	21.769208536403333
36-37	21.974090250148958	27.47320840659978	28.82810842684315	21.72459291640811
38-39	21.828015533300512	27.73998053768427	28.766755870331647	21.665248058683574
40-41	22.168262504639884	27.398956301702555	28.53119371322746	21.901587480430102
42-43	21.963675993164404	27.548310371476642	28.52352585372714	21.964487781631814
44-45	22.12781065609213	27.78818195078366	28.173957973130154	21.910049419994056
46-47	22.22313418850635	27.647432596707727	27.923686383459835	22.205746831326096
48-49	22.06910179692165	27.818506474445165	27.97117250247365	22.141219226159535
50-51	22.145583081910416	28.060844874430035	27.708494254477152	22.085077789182396
52-53	22.372320359740225	27.739726981538425	27.625241809585454	22.262710849135896
54-55	22.26910487901092	27.72315978447623	27.77058253861125	22.237152797901597
56-57	22.19450401642333	28.002829328207547	27.628115745908154	22.17455090946097
58-59	22.338454418054404	27.81986566414471	27.622921385771665	22.218758532029213
60-61	22.282732255742214	27.846395340955677	27.735407417050933	22.135464986251176
62-63	22.273597170908438	28.00522092050159	27.63838477546372	22.082797133126252
64-65	22.350898942304923	27.75984759733932	27.526545433603456	22.362708026752305
66-67	22.230986690301187	27.845411897739947	27.589474488470533	22.33412692348833
68-69	22.271508915198783	27.93231638550458	27.56320982919395	22.232964870102684
70-71	22.338402107426123	27.565034902907566	27.59996207409413	22.49660091557218
72-73	22.297619694708022	27.674725545654265	27.561677457630037	22.465977302007676
74-75	22.163539914295953	27.866400074629894	27.57617692655675	22.39388308451741
76-77	22.428146943417424	27.56349228974941	27.415747056888627	22.592613709944544
78-79	22.197056267356405	27.66169823342612	27.597620991211496	22.543624508005976
80-81	22.31291261007988	27.76681417654271	27.47204216490204	22.44823104847537
82-83	22.401483415523533	27.612732057050028	27.55858414947141	22.42720037795503
84-85	22.45080170451448	27.608477681016797	27.55858062033043	22.382139994138296
86-87	22.323009122037	27.660835286799262	27.54995025450861	22.46620533665513
88-89	22.389404464965025	27.500673751506277	27.526241233483212	22.583680550045486
90-91	22.428841263639487	27.541122250326495	27.586133858201983	22.443902627832035
92-93	22.373207994478538	27.61230712588934	27.610369747010495	22.404115132621627
94-95	22.387002109357393	27.468631953988087	27.66595549998644	22.47841043666808
96-97	22.428368689002962	27.762814360076394	27.737517412125868	22.07129953879478
98-99	23.489929088058968	29.84357239224063	27.252177038383827	19.41432148131657
100	22.63255198889492	30.183248682057584	21.56198867576404	25.622210653283457
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1547.0
1	1401.0
2	2172.5
3	3339.0
4	4823.5
5	8288.0
6	11185.0
7	12879.5
8	13707.0
9	13315.5
10	12186.5
11	10920.0
12	11643.0
13	14790.0
14	22537.0
15	34836.0
16	46365.0
17	53547.5
18	55002.5
19	51982.0
20	47273.0
21	42388.5
22	39279.5
23	40425.5
24	46244.5
25	56824.0
26	71357.0
27	88300.0
28	107593.5
29	129496.0
30	151848.0
31	173855.0
32	195706.0
33	215777.5
34	235565.0
35	254510.0
36	273963.0
37	293799.5
38	310004.5
39	323469.0
40	336974.5
41	347043.5
42	353147.5
43	359395.0
44	364105.0
45	369679.0
46	373583.5
47	373450.5
48	372492.0
49	368669.5
50	362279.0
51	352342.0
52	338035.0
53	320489.5
54	300680.0
55	278128.0
56	254920.5
57	231128.5
58	205256.0
59	177454.0
60	147780.0
61	119512.5
62	94558.0
63	72641.5
64	53860.0
65	40147.0
66	30214.0
67	22807.0
68	17301.5
69	12466.5
70	8734.5
71	6406.0
72	4593.0
73	3138.5
74	2170.0
75	1474.5
76	996.5
77	660.5
78	442.5
79	295.5
80	213.0
81	165.5
82	122.5
83	89.5
84	68.0
85	50.0
86	39.0
87	32.0
88	22.0
89	14.5
90	10.0
91	5.5
92	4.5
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0915403981830218E-5
2	2.1830807963660436E-5
3	2.401388876002648E-4
4	9.933017623465499E-4
5	7.640782787281154E-5
6	8.732323185464175E-5
7	2.1830807963660436E-5
8	1.200694438001324E-4
9	5.894318150188318E-4
10-11	0.003793102883686001
12-13	2.2376578162751947E-4
14-15	0.0012661868618923054
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	198.0
20-21	647.0
22-23	1428.0
24-25	3667.0
26-27	4372.0
28-29	7737.0
30-31	15795.0
32-33	19875.0
34-35	28927.0
36-37	39175.0
38-39	45703.0
40-41	49139.0
42-43	59520.0
44-45	71015.0
46-47	68711.0
48-49	61913.0
50-51	55719.0
52-53	55398.0
54-55	59204.0
56-57	62434.0
58-59	62596.0
60-61	61961.0
62-63	62101.0
64-65	66486.0
66-67	70726.0
68-69	72119.0
70-71	71272.0
72-73	70489.0
74-75	73019.0
76-77	73080.0
78-79	77733.0
80-81	79134.0
82-83	78632.0
84-85	81049.0
86-87	86411.0
88-89	92513.0
90-91	97464.0
92-93	103424.0
94-95	136615.0
96-97	455812.0
98-99	1641446.0
100-101	4836706.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.12106029891444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.1346947534673	70.68458827223525
2	8.528895780011007	13.837461377068596
3	2.3410789586184757	5.697324220998276
4	0.9141215459134062	2.966180361863133
5	0.4257198207424372	1.726742162444515
6	0.21679133520409533	1.0551805785224153
7	0.1238236724349284	0.7031295318618799
8	0.08407253796770199	0.5456042737568582
9	0.051832682582697964	0.37842499523209805
>10	0.1749221334364174	2.1303106304346375
>50	0.003508124756868462	0.18658470611663788
>100	5.38654864612953E-4	0.08846888946558742
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0915403981830218E-5
2	0.0	3.274621194549065E-5	0.0	0.0	1.0915403981830218E-5
3	0.0	3.274621194549065E-5	0.0	0.0	1.0915403981830218E-5
4	0.0	3.274621194549065E-5	0.0	0.0	2.1830807963660436E-5
5	0.0	3.274621194549065E-5	0.0	0.0	2.1830807963660436E-5
6	0.0	3.274621194549065E-5	0.0	0.0	2.1830807963660436E-5
7	0.0	4.366161592732087E-5	0.0	0.0	2.1830807963660436E-5
8	0.0	4.366161592732087E-5	0.0	0.0	2.1830807963660436E-5
9	0.0	1.4190025176379284E-4	0.0	1.0915403981830218E-5	3.274621194549065E-5
10-11	0.0	1.4735795375470795E-4	0.0	1.0915403981830218E-5	3.274621194549065E-5
12-13	0.0	1.5281565574562305E-4	0.0	1.0915403981830218E-5	3.274621194549065E-5
14-15	0.0	2.947159075094159E-4	0.0	1.6373105972745326E-5	4.366161592732087E-5
16-17	0.0	3.383775234367368E-4	0.0	2.1830807963660436E-5	6.00347219000662E-5
18-19	0.0	3.383775234367368E-4	0.0	2.1830807963660436E-5	6.54924238909813E-5
20-21	0.0	3.383775234367368E-4	0.0	2.7288509954575543E-5	6.54924238909813E-5
22-23	0.0	3.8749684135497276E-4	0.0	4.366161592732087E-5	8.732323185464175E-5
24-25	0.0	4.0932764931863317E-4	0.0	6.00347219000662E-5	1.0915403981830218E-4
26-27	0.0	4.2570075529137853E-4	0.0	1.801041657001986E-4	1.0915403981830218E-4
28-29	0.0	4.2570075529137853E-4	0.0	5.948895170097469E-4	1.0915403981830218E-4
30-31	0.0	4.639046692277843E-4	0.0	0.001893822590847543	1.0915403981830218E-4
32-33	0.0	4.802777752005296E-4	0.0	0.004196972831013719	1.0915403981830218E-4
34-35	0.0	5.45770199091511E-4	0.0	0.007127758800135133	1.200694438001324E-4
36-37	0.0	6.112626229824922E-4	0.0	0.010555195650429821	1.4190025176379284E-4
38-39	0.0	6.330934309461527E-4	0.0	0.015205157746689495	1.4190025176379284E-4
40-41	0.0	6.98585854837134E-4	0.0	0.020106174134531266	1.4190025176379284E-4
42-43	0.0	7.586205767372001E-4	0.0	0.024947155800472964	1.4190025176379284E-4
44-45	0.0	8.186552986372663E-4	0.0	0.030202922817724215	1.4190025176379284E-4
46-47	0.0	8.186552986372663E-4	0.0	0.03502753137769317	1.4190025176379284E-4
48-49	0.0	8.186552986372663E-4	0.0	0.040179602057117035	1.4190025176379284E-4
50-51	0.0	8.786900205373326E-4	0.0	0.0447204101135584	1.4190025176379284E-4
52-53	0.0	8.950631265100779E-4	0.0	0.04899379077244494	1.6373105972745328E-4
54-55	0.0	9.114362324828232E-4	0.0	0.05316347509350408	1.8556186769111372E-4
56-57	0.0	9.387247424373988E-4	0.0	0.05761695991809081	1.8556186769111372E-4
58-59	0.0	9.550978484101441E-4	0.0	0.061868509769013674	1.9101956968202883E-4
60-61	0.0	9.769286563738044E-4	0.0	0.06604910949405465	1.9647727167294393E-4
62-63	0.0	9.933017623465499E-4	0.0	0.07054079823257778	2.0739267565477416E-4
64-65	0.0	0.0011242866101285125	0.0	0.07479780578549158	2.0739267565477416E-4
66-67	0.0	0.0011352020141103428	0.0	0.07904389793442353	2.1285037764568927E-4
68-69	0.0	0.0011515751200830882	0.0	0.08391762581231071	2.1830807963660437E-4
70-71	0.0	0.0011570328220740032	0.0	0.08866036884241596	2.1830807963660437E-4
72-73	0.0	0.0011570328220740032	0.0	0.09230611377234724	2.2922348361843458E-4
74-75	0.0	0.0011734059280467486	0.0	0.09543337701314161	2.401388876002648E-4
76-77	0.0	0.0012170675439740694	0.0	0.09852789404199047	2.455965895911799E-4
78-79	0.0	0.0012334406499468146	0.0	0.10024161246713781	2.51054291582095E-4
80-81	0.0	0.0012334406499468146	0.0	0.10087470589808396	2.565119935730101E-4
82-83	0.0	0.0012334406499468146	0.0	0.1013549836732845	2.619696955639252E-4
84-85	0.0	0.0012334406499468146	0.0	0.10156783405093017	2.619696955639252E-4
86-87	0.0	0.0012443560539286448	0.0	0.10161149566685751	2.619696955639252E-4
88	0.0	0.001353510093746947	0.0	0.10161149566685751	2.619696955639252E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12490	0.0	46.49986	1
GTATCAA	22215	0.0	36.782738	1
ATCAACG	25465	0.0	31.414358	3
TCAACGC	26415	0.0	30.366743	4
CAACGCA	26970	0.0	29.67936	5
AACGCAG	27050	0.0	29.670715	6
TATCAAC	27565	0.0	29.20783	2
ACGCAGA	27585	0.0	29.110813	7
CGCAGAG	32015	0.0	25.056011	8
GTACATG	27265	0.0	21.773386	1
GCAGAGT	37655	0.0	21.132566	9
TACATGG	27215	0.0	21.05674	2
ACATGGG	26705	0.0	20.752144	3
GAGTACT	22020	0.0	19.723114	12-13
CAGAGTA	36695	0.0	18.60474	10-11
AGAGTAC	33700	0.0	18.367933	10-11
CATGGGG	20790	0.0	17.950314	4
GTGGTAT	6605	0.0	17.923866	1
GTACTTT	25065	0.0	17.328299	14-15
AGTACTT	24130	0.0	16.60303	12-13
>>END_MODULE