FastQCFastQC Report
Fri 27 May 2016
SRR2139218_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139218_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11525520
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158100.051.3965641
GTATCAA288950.037.7042771
TCAACGC348050.030.5551054
ATCAACG350300.030.3834933
CAACGCA358650.029.6663935
TATCAAC366850.029.3252622
AACGCAG364600.029.2548226
ACGCAGA418550.025.473427
CGCAGAG422850.025.1938488
GTACATG327950.021.7929481
GTGGTAT79800.021.5477681
GCAGAGT496350.021.2945149
TACATGG328050.021.1277432
TGGTATC76950.020.6842212
ACATGGG333000.020.2575913
GAGTACT317400.020.1353612-13
CAGAGTA480450.019.24571810-11
AGAGTAC449000.019.1697910-11
CATGGGG239400.018.325134
GTACTTT351800.018.12910514-15
Produced by FastQC (version 0.11.5)