##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139218_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11525520
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55568043784575	33.0	33.0	33.0	33.0	33.0
2	32.572903088103615	33.0	33.0	33.0	33.0	33.0
3	32.56739756644386	33.0	33.0	33.0	33.0	33.0
4	36.4801155175645	37.0	37.0	37.0	37.0	37.0
5	36.516894682409124	37.0	37.0	37.0	37.0	37.0
6	36.512121014930344	37.0	37.0	37.0	37.0	37.0
7	36.4941453400801	37.0	37.0	37.0	37.0	37.0
8	36.48787143660329	37.0	37.0	37.0	37.0	37.0
9	36.50556799172619	37.0	37.0	37.0	37.0	37.0
10-11	36.46036695090547	37.0	37.0	37.0	37.0	37.0
12-13	36.4429823122948	37.0	37.0	37.0	37.0	37.0
14-15	38.43131845678113	40.0	37.0	40.0	37.0	40.0
16-17	38.40223642837807	40.0	37.0	40.0	37.0	40.0
18-19	38.432748587482386	40.0	37.0	40.0	37.0	40.0
20-21	38.430049843408554	40.0	37.0	40.0	37.0	40.0
22-23	38.43020716585872	40.0	37.0	40.0	37.0	40.0
24-25	38.406237949533136	40.0	37.0	40.0	37.0	40.0
26-27	38.290340662075245	40.0	37.0	40.0	37.0	40.0
28-29	38.23588398963405	40.0	37.0	40.0	37.0	40.0
30-31	38.15901396324173	40.0	37.0	40.0	37.0	40.0
32-33	38.08335676246172	40.0	37.0	40.0	33.0	40.0
34-35	38.064564680950724	40.0	37.0	40.0	33.0	40.0
36-37	38.00642966083471	40.0	37.0	40.0	33.0	40.0
38-39	37.96098236832475	40.0	37.0	40.0	33.0	40.0
40-41	37.92476954592698	40.0	37.0	40.0	33.0	40.0
42-43	37.86492433506811	40.0	37.0	40.0	33.0	40.0
44-45	37.793457995217864	40.0	37.0	40.0	33.0	40.0
46-47	37.749855787737644	40.0	37.0	40.0	33.0	40.0
48-49	37.72734217771132	40.0	37.0	40.0	33.0	40.0
50-51	37.65442534520069	40.0	37.0	40.0	33.0	40.0
52-53	37.55113783567472	40.0	37.0	40.0	33.0	40.0
54-55	37.374998107444696	40.0	37.0	40.0	33.0	40.0
56-57	37.266123271069446	40.0	37.0	40.0	33.0	40.0
58-59	37.15254003561003	37.0	37.0	40.0	33.0	40.0
60-61	37.0175170028784	37.0	37.0	40.0	33.0	40.0
62-63	36.8566756764602	37.0	37.0	40.0	33.0	40.0
64-65	36.66731412579732	37.0	37.0	40.0	33.0	40.0
66-67	36.46010563362479	37.0	37.0	40.0	33.0	40.0
68-69	36.24402477403612	37.0	37.0	40.0	33.0	40.0
70-71	35.983654975304034	37.0	37.0	38.5	33.0	40.0
72-73	35.78052525520011	37.0	33.0	37.0	33.0	40.0
74-75	35.58164236965456	37.0	33.0	37.0	33.0	40.0
76-77	33.76848301150581	35.0	33.0	37.0	27.0	37.0
78-79	35.05067369218179	37.0	33.0	37.0	33.0	37.0
80-81	35.09999002805117	37.0	33.0	37.0	33.0	37.0
82-83	34.962311958232085	37.0	33.0	37.0	33.0	37.0
84-85	34.81342035829486	37.0	33.0	37.0	33.0	37.0
86-87	34.709350392860614	37.0	33.0	37.0	33.0	37.0
88-89	34.585222665170406	37.0	33.0	37.0	33.0	37.0
90-91	34.520158458185946	37.0	33.0	37.0	33.0	37.0
92-93	34.457053125348196	37.0	33.0	37.0	33.0	37.0
94-95	34.47091336406032	37.0	33.0	37.0	33.0	37.0
96-97	34.45300944246035	37.0	33.0	37.0	33.0	37.0
98-99	34.46470669286222	37.0	33.0	37.0	33.0	37.0
100	34.474443544998216	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	26.0
20	72.0
21	485.0
22	1866.0
23	5247.0
24	11797.0
25	23928.0
26	41358.0
27	65686.0
28	96801.0
29	135447.0
30	182255.0
31	240853.0
32	316767.0
33	421801.0
34	573492.0
35	821070.0
36	1406160.0
37	3290594.0
38	3810435.0
39	79375.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.691420430488165	20.25175436769881	14.417076192657685	23.639749009155338
2	14.490296316348417	21.29658358147832	41.712443343120306	22.500676759052954
3	17.912172292443206	26.512365602593203	30.65166690960581	24.92379519535778
4	12.264515683452439	18.375637591975146	39.47774115527137	29.88210556930105
5	13.219429578882341	37.83030179983202	35.732201236907315	13.218067384378319
6	29.314478687530077	38.34967419217863	19.08755242503019	13.248294695261102
7	25.914761329640655	33.01589863190554	23.48188194545669	17.587458092997103
8	24.10601864384427	36.729683346174404	21.40242696208067	17.76187104790066
9	25.396728303798877	16.957308650715976	21.46510526206193	36.18085778342322
10-11	23.81673893818824	26.940801849466066	29.20030497031858	20.04215424202711
12-13	24.845746602581613	24.596035439975093	29.25310675895118	21.30511119849211
14-15	21.975068291772317	25.918800738909447	27.028049917904973	25.07808105141326
16-17	21.388314800546958	28.982189089949955	27.69379169009294	21.935704419410143
18-19	21.370428405833316	28.066811736043146	29.582062241009517	20.98069761711402
20-21	22.20982327817148	27.231963939810022	29.541213040149067	21.01699974186943
22-23	21.859740958438593	27.179891901507368	29.391036480800746	21.56933065925329
24-25	21.68876451166462	27.323684609307914	29.42329233155047	21.564258547476996
26-27	21.654267459961225	27.509387309312817	29.30226137568251	21.53408385504345
28-29	21.58075971184455	27.579917468489377	29.341250090818882	21.49807272884719
30-31	21.900377255303933	27.400081364104278	29.21725793407578	21.48228344651601
32-33	21.678779990615205	27.543040910185844	29.13599319393525	21.642185905263702
34-35	21.82387514394217	27.598841742473052	29.115804703134295	21.461478410450482
36-37	21.799735133445242	27.582023071395085	29.011858722580104	21.60638307257957
38-39	21.638844948385735	27.729681917668575	29.067428990477755	21.564044143467935
40-41	21.93587226480726	27.58262483587525	28.93156327613209	21.5499396231854
42-43	21.844681588755613	27.703721834137635	28.674078638381406	21.777517938725346
44-45	21.966540694484017	27.7985435413297	28.456940904671686	21.777974859514597
46-47	21.98806954137443	27.816099204514156	28.214243621075042	21.981587633036373
48-49	21.902951245038977	28.0058919926949	28.11742893069798	21.973727831568144
50-51	21.953408313931853	28.099287626614505	27.999084199548214	21.94821985990543
52-53	22.146648011688423	27.985708714882595	27.862048409686647	22.005594863742335
54-55	22.1198909710082	27.911872020514544	27.897177203938323	22.071059804538933
56-57	22.112960820204346	28.044966157525902	27.849507997074614	21.992565025195134
58-59	22.07875345800308	28.090232040487845	27.87188814152493	21.959126359984147
60-61	22.146552596844288	28.023644076610378	27.89617882951974	21.933624497025594
62-63	22.075314284145296	28.10751335853716	27.843682017652082	21.973490339665457
64-65	22.07334786968356	28.07131537959315	27.77097446908739	22.0843622816359
66-67	22.13382654860548	28.070512283456427	27.67716472271136	22.118496445226732
68-69	22.073657112147675	28.076086624013307	27.7770759523645	22.073180311474523
70-71	22.08640076887026	27.909607371102602	27.804687983389297	22.19930387663784
72-73	22.16319442467664	27.931720545106963	27.718122991946426	22.18696203826997
74-75	22.017442183078185	28.073132780082986	27.688049499670147	22.22137553716868
76-77	22.129957347062067	27.99474237690496	27.645513291366854	22.229786984666124
78-79	22.09471190943107	28.007264631273294	27.63766075550015	22.260362703795483
80-81	22.10751880728897	28.03493177022256	27.648746766931488	22.208802655556987
82-83	22.111609808240026	28.03194443202669	27.704046102662065	22.152399657071218
84-85	22.24408123585314	28.00870636025333	27.604013785754972	22.143198618138555
86-87	22.10369868008422	28.012677377027146	27.69508093413156	22.188543008757073
88-89	22.13511691527399	27.997834767203027	27.642596158620243	22.224452158902736
90-91	22.13959484501982	28.048917213429338	27.661078690814335	22.15040925073651
92-93	22.19091839513244	28.01389449655663	27.67307349226768	22.12211361604325
94-95	22.05870830155062	28.070334315667296	27.69060606625785	22.180351316524234
96-97	22.11164023425443	28.198941641268867	27.747309505171593	21.942108619305113
98-99	22.323852086454597	28.928697295319623	26.84868406754856	21.898766550677216
100	20.421762148290117	28.65040500394625	27.98119076054335	22.946642087220283
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3232.0
1	2759.5
2	3524.0
3	5047.0
4	6887.5
5	11186.5
6	14845.0
7	17511.0
8	19509.0
9	19371.0
10	17491.5
11	15496.0
12	16558.5
13	21843.0
14	33975.5
15	50464.0
16	63908.5
17	71870.0
18	73195.0
19	68715.5
20	61778.5
21	54651.0
22	50056.5
23	51684.5
24	59244.5
25	72547.0
26	91465.5
27	112142.0
28	135086.0
29	160550.0
30	185362.0
31	210970.0
32	237839.0
33	264151.0
34	289814.5
35	313370.0
36	338461.0
37	361158.0
38	378581.5
39	393699.0
40	407548.5
41	420505.0
42	429106.0
43	436849.0
44	443715.0
45	449127.5
46	450925.0
47	449385.0
48	448966.5
49	443747.5
50	432879.5
51	418081.0
52	399339.0
53	379383.0
54	357386.0
55	330855.0
56	303055.5
57	271885.5
58	238968.0
59	206094.5
60	170002.5
61	136079.0
62	106265.0
63	80400.0
64	58889.0
65	42571.0
66	30952.0
67	22846.5
68	16867.0
69	11898.0
70	8183.0
71	5598.5
72	3713.0
73	2413.0
74	1559.0
75	1031.0
76	699.5
77	477.5
78	330.5
79	235.5
80	168.0
81	124.0
82	97.0
83	68.5
84	47.5
85	34.5
86	25.5
87	18.0
88	13.0
89	11.0
90	11.0
91	10.5
92	7.0
93	4.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.001075873366234235
5	0.0
6	1.2146957360709104E-4
7	0.0
8	0.0
9	0.0
10-11	0.004025848725263589
12-13	3.470559245916887E-5
14-15	0.001357856304964982
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	220.0
20-21	741.0
22-23	1656.0
24-25	3615.0
26-27	4522.0
28-29	6758.0
30-31	13278.0
32-33	16591.0
34-35	23260.0
36-37	29605.0
38-39	36655.0
40-41	42589.0
42-43	54284.0
44-45	67016.0
46-47	64559.0
48-49	55833.0
50-51	48148.0
52-53	46784.0
54-55	50524.0
56-57	53370.0
58-59	55139.0
60-61	55985.0
62-63	57028.0
64-65	61932.0
66-67	66819.0
68-69	69494.0
70-71	70620.0
72-73	70037.0
74-75	74267.0
76-77	72626.0
78-79	80564.0
80-81	83885.0
82-83	83893.0
84-85	86534.0
86-87	93360.0
88-89	101275.0
90-91	108483.0
92-93	116915.0
94-95	145589.0
96-97	407431.0
98-99	55451.0
100-101	8888185.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.59175737366724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.1431408219004	72.79896900915382
2	8.083701086535287	13.352941576407455
3	2.077156771749964	5.1466808435832885
4	0.7742244438399015	2.557782296735504
5	0.360848474374649	1.4901554822104495
6	0.1813117435839439	0.8984913321048997
7	0.11041945914098283	0.6383816025092532
8	0.06692752000203836	0.44221291949036906
9	0.0451819108644183	0.33584880778134263
>10	0.15207944236466014	1.9575478115406153
>50	0.003988470804702735	0.21408044868751808
>100	9.245145731895299E-4	0.11363130638336542
>500	7.62741549936523E-5	0.03675792264310648
>1k	1.9066110839124788E-5	0.01651864076908179
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.7352796229584435E-5	0.0	0.0	0.0
3	0.0	1.7352796229584435E-5	0.0	0.0	0.0
4	0.0	6.0734786803545525E-5	0.0	8.676398114792217E-6	0.0
5	0.0	6.941118491833774E-5	0.0	8.676398114792217E-6	0.0
6	5.2058388688753304E-5	6.941118491833774E-5	0.0	8.676398114792217E-6	0.0
7	5.2058388688753304E-5	6.941118491833774E-5	0.0	8.676398114792217E-6	2.6029194344376652E-5
8	5.2058388688753304E-5	8.676398114792218E-5	0.0	1.7352796229584435E-5	3.470559245916887E-5
9	7.808758303312995E-5	1.474987679514677E-4	0.0	2.6029194344376652E-5	3.470559245916887E-5
10-11	7.808758303312995E-5	1.474987679514677E-4	0.0	3.470559245916887E-5	3.470559245916887E-5
12-13	7.808758303312995E-5	1.474987679514677E-4	0.0	3.470559245916887E-5	3.470559245916887E-5
14-15	8.676398114792218E-5	1.99557156640221E-4	0.0	6.0734786803545525E-5	3.470559245916887E-5
16-17	1.3448417077927937E-4	1.99557156640221E-4	0.0	8.676398114792218E-5	3.470559245916887E-5
18-19	1.3882236983667548E-4	2.2124815192720157E-4	0.0	8.676398114792218E-5	3.470559245916887E-5
20-21	1.4316056889407159E-4	2.429391472141821E-4	0.0	9.110218020531829E-5	4.338199057396109E-5
22-23	1.474987679514677E-4	2.6463014250116264E-4	0.0	1.0845497643490272E-4	4.338199057396109E-5
24-25	1.474987679514677E-4	3.166885311899159E-4	0.0	1.1713137454969494E-4	4.338199057396109E-5
26-27	1.474987679514677E-4	3.297031283621043E-4	0.0	1.3014597172188326E-4	4.338199057396109E-5
28-29	1.474987679514677E-4	3.297031283621043E-4	0.0	2.1257175381240935E-4	4.338199057396109E-5
30-31	1.474987679514677E-4	3.7308511893606537E-4	0.0	6.203624652076435E-4	4.338199057396109E-5
32-33	1.474987679514677E-4	3.7308511893606537E-4	0.0	0.0013752091011945665	4.338199057396109E-5
34-35	1.561751660662599E-4	4.077907113952342E-4	0.0	0.002381671282510464	4.338199057396109E-5
36-37	1.7352796229584436E-4	4.468345029117992E-4	0.0	0.0036787928006719005	4.338199057396109E-5
38-39	1.7352796229584436E-4	4.511727019691953E-4	0.0	0.00541841062268774	5.2058388688753304E-5
40-41	1.908807585254288E-4	4.5984910008398756E-4	0.0	0.007322880008884631	5.2058388688753304E-5
42-43	1.908807585254288E-4	4.815400953709681E-4	0.0	0.009839035462174375	5.2058388688753304E-5
44-45	1.908807585254288E-4	5.335984840597214E-4	0.0	0.012355190915464118	5.2058388688753304E-5
46-47	1.908807585254288E-4	5.639658774614941E-4	0.0	0.01514031471031242	5.2058388688753304E-5
48-49	1.908807585254288E-4	5.683040765188902E-4	0.0	0.017864703718357175	5.2058388688753304E-5
50-51	1.99557156640221E-4	6.160242661502474E-4	0.0	0.02069320950377944	5.2058388688753304E-5
52-53	1.99557156640221E-4	6.160242661502474E-4	0.0	0.02320068855895439	5.2058388688753304E-5
54-55	1.99557156640221E-4	6.247006642650396E-4	0.0	0.025925077566999147	5.2058388688753304E-5
56-57	1.99557156640221E-4	6.594062567242086E-4	0.0	0.029261152642136752	5.2058388688753304E-5
58-59	1.99557156640221E-4	6.637444557816047E-4	0.0	0.03242369975497852	5.2058388688753304E-5
60-61	2.0823355475501322E-4	6.724208538963969E-4	0.0	0.03584220061220665	5.2058388688753304E-5
62-63	2.429391472141821E-4	7.027882472981697E-4	0.0	0.03905246791467977	5.2058388688753304E-5
64-65	2.429391472141821E-4	8.069050246756762E-4	0.0	0.042657511331375936	5.2058388688753304E-5
66-67	2.6029194344376653E-4	8.112432237330723E-4	0.0	0.04653152308963066	5.2058388688753304E-5
68-69	2.6896834155855874E-4	8.502870152496373E-4	0.0	0.05023200688558954	6.0734786803545525E-5
70-71	2.8198293873074706E-4	8.8065440865141E-4	0.0	0.053845726700400505	6.0734786803545525E-5
72-73	3.2102673024731203E-4	9.023454039383906E-4	0.0	0.05666555608770797	6.0734786803545525E-5
74-75	3.2102673024731203E-4	9.283745982827673E-4	0.0	0.05901685997681666	6.507298586094163E-5
76-77	3.2970312836210424E-4	0.0010108003803732933	0.0	0.0613247818753514	6.941118491833774E-5
78-79	3.5139412364908483E-4	0.0010498441718898583	0.0	0.06303837050302286	7.374938397573385E-5
80-81	3.5573232270648094E-4	0.0010498441718898583	0.0	0.0636674093663453	8.242578209052606E-5
82-83	3.5573232270648094E-4	0.0010585205700046505	0.0	0.06404917088339615	8.676398114792218E-5
84-85	3.5573232270648094E-4	0.001071535167176839	0.0	0.06422269884569198	9.110218020531829E-5
86-87	3.5573232270648094E-4	0.0011019025605786116	0.0	0.06431380102589732	1.0411677737750661E-4
88	3.6440872082127315E-4	0.0011713137454969493	0.0	0.0643181392249547	1.1279317549229882E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15810	0.0	51.396564	1
GTATCAA	28895	0.0	37.704277	1
TCAACGC	34805	0.0	30.555105	4
ATCAACG	35030	0.0	30.383493	3
CAACGCA	35865	0.0	29.666393	5
TATCAAC	36685	0.0	29.325262	2
AACGCAG	36460	0.0	29.254822	6
ACGCAGA	41855	0.0	25.47342	7
CGCAGAG	42285	0.0	25.193848	8
GTACATG	32795	0.0	21.792948	1
GTGGTAT	7980	0.0	21.547768	1
GCAGAGT	49635	0.0	21.294514	9
TACATGG	32805	0.0	21.127743	2
TGGTATC	7695	0.0	20.684221	2
ACATGGG	33300	0.0	20.257591	3
GAGTACT	31740	0.0	20.13536	12-13
CAGAGTA	48045	0.0	19.245718	10-11
AGAGTAC	44900	0.0	19.16979	10-11
CATGGGG	23940	0.0	18.32513	4
GTACTTT	35180	0.0	18.129105	14-15
>>END_MODULE