FastQCFastQC Report
Fri 27 May 2016
SRR2139217_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139217_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9599505
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131400.053.6601141
GTATCAA240500.038.465881
TCAACGC292650.031.1434754
ATCAACG296450.030.7292023
CAACGCA300450.030.3381165
TATCAAC306750.029.8702092
AACGCAG307450.029.7336066
ACGCAGA348800.026.208577
CGCAGAG353850.025.835348
GTACATG297250.022.4572541
TACATGG292000.022.1045382
GCAGAGT414550.021.915249
ACATGGG295350.021.0610373
GAGTACT254900.020.66136212-13
GTGGTAT63750.020.097031
CAGAGTA405350.019.73091910-11
TGGTATC59700.019.6843642
AGAGTAC375900.019.51935610-11
CATGGGG209300.018.680584
GTACTTT285650.018.3754114-15
Produced by FastQC (version 0.11.5)