##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139217_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9599505
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6465344827676	33.0	33.0	33.0	33.0	33.0
2	32.651174722029936	33.0	33.0	33.0	33.0	33.0
3	32.651322125463764	33.0	33.0	33.0	33.0	33.0
4	36.56310070154659	37.0	37.0	37.0	37.0	37.0
5	36.58612813889883	37.0	37.0	37.0	37.0	37.0
6	36.57131779190698	37.0	37.0	37.0	37.0	37.0
7	36.53835765489991	37.0	37.0	37.0	37.0	37.0
8	36.53574293674517	37.0	37.0	37.0	37.0	37.0
9	36.57302537995449	37.0	37.0	37.0	37.0	37.0
10-11	36.50271987982714	37.0	37.0	37.0	37.0	37.0
12-13	36.50050148419111	37.0	37.0	37.0	37.0	37.0
14-15	38.601576279193566	40.0	37.0	40.0	37.0	40.0
16-17	38.54428342919765	40.0	37.0	40.0	37.0	40.0
18-19	38.563700367883555	40.0	37.0	40.0	37.0	40.0
20-21	38.54844476731893	40.0	37.0	40.0	37.0	40.0
22-23	38.55836752259275	40.0	37.0	40.0	37.0	40.0
24-25	38.54637841086482	40.0	37.0	40.0	37.0	40.0
26-27	38.44875695173665	40.0	37.0	40.0	37.0	40.0
28-29	38.387446587850874	40.0	37.0	40.0	37.0	40.0
30-31	38.329943918702874	40.0	37.0	40.0	37.0	40.0
32-33	38.26655650920512	40.0	37.0	40.0	37.0	40.0
34-35	38.23235831056091	40.0	37.0	40.0	37.0	40.0
36-37	38.17580517482918	40.0	37.0	40.0	37.0	40.0
38-39	38.12809947145173	40.0	37.0	40.0	37.0	40.0
40-41	38.09613221216964	40.0	37.0	40.0	35.0	40.0
42-43	38.03948967734887	40.0	37.0	40.0	33.0	40.0
44-45	37.97481569788941	40.0	37.0	40.0	33.0	40.0
46-47	37.93255552897469	40.0	37.0	40.0	33.0	40.0
48-49	37.90859083165288	40.0	37.0	40.0	33.0	40.0
50-51	37.83436200765429	40.0	37.0	40.0	33.0	40.0
52-53	37.727248140853874	40.0	37.0	40.0	33.0	40.0
54-55	37.53503625838312	40.0	37.0	40.0	33.0	40.0
56-57	37.45959553406085	40.0	37.0	40.0	33.0	40.0
58-59	37.342045450841084	40.0	37.0	40.0	33.0	40.0
60-61	37.20338639190962	37.0	37.0	40.0	33.0	40.0
62-63	37.03880082479068	37.0	37.0	40.0	33.0	40.0
64-65	36.84924132473249	37.0	37.0	40.0	33.0	40.0
66-67	36.64108589883621	37.0	37.0	40.0	33.0	40.0
68-69	36.43568893595173	37.0	37.0	40.0	33.0	40.0
70-71	36.16629755123968	37.0	37.0	38.5	33.0	40.0
72-73	35.94952251185239	37.0	37.0	37.0	33.0	40.0
74-75	35.75919963127595	37.0	35.0	37.0	33.0	40.0
76-77	34.133648963020654	35.0	33.0	37.0	30.0	37.0
78-79	35.21193809904479	37.0	33.0	37.0	33.0	37.0
80-81	35.2659052598207	37.0	33.0	37.0	33.0	37.0
82-83	35.14285442311498	37.0	33.0	37.0	33.0	37.0
84-85	34.978818555368505	37.0	33.0	37.0	33.0	37.0
86-87	34.89745687712313	37.0	33.0	37.0	33.0	37.0
88-89	34.795916281401254	37.0	33.0	37.0	33.0	37.0
90-91	34.68727966817032	37.0	33.0	37.0	33.0	37.0
92-93	34.65987857621719	37.0	33.0	37.0	33.0	37.0
94-95	34.68937078875655	37.0	33.0	37.0	33.0	37.0
96-97	34.68114425987537	37.0	33.0	37.0	33.0	37.0
98-99	34.6744908103842	37.0	33.0	37.0	33.0	37.0
100	34.68518350730247	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	0.0
17	2.0
18	2.0
19	6.0
20	63.0
21	346.0
22	1222.0
23	3592.0
24	8390.0
25	16515.0
26	28903.0
27	46156.0
28	68387.0
29	97061.0
30	132569.0
31	176935.0
32	235424.0
33	314891.0
34	434728.0
35	631122.0
36	1111866.0
37	2784007.0
38	3435301.0
39	72015.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.782310650392915	20.038085297106463	14.367657499006459	23.811946553494163
2	14.534416097496694	21.004645552036276	41.85567901678264	22.605259333684394
3	18.09046403955204	26.329409693520656	30.43521514911446	25.144911117812846
4	12.304894887809319	18.22260626980245	39.11691279914954	30.355586043238684
5	13.303571382066053	37.81727286979902	35.521435740697044	13.357720007437884
6	29.594062190739102	38.01698005104433	19.001239647898327	13.387718110318245
7	26.247020028636893	32.79782655459839	23.213321936912372	17.741831479852348
8	24.298607063593384	36.727737523966084	21.190530136710173	17.783125275730363
9	25.570370555565102	16.93238349269051	21.195749155815847	36.30149679592854
10-11	24.124435323088303	26.820059980440597	28.895101944611202	20.160402751859902
12-13	25.101678435764935	24.418085789541095	28.95382558066675	21.526410194027225
14-15	22.12257362536495	25.744400229166832	26.791553455339994	25.341472690128224
16-17	21.629953836161345	28.822626791693946	27.40544955182585	22.14196982031886
18-19	21.63451657142738	27.89180796301476	29.26211299436794	21.21156247118992
20-21	22.4373933742034	27.046575924130174	29.23884892033869	21.27718178132774
22-23	22.130619413911383	27.029413926487244	29.06435862733864	21.77560803226274
24-25	21.91203263985962	27.17851316915802	29.132304280933703	21.777149910048657
26-27	21.85735526568825	27.339120256367362	29.0583638079663	21.745160669978095
28-29	21.787627916264032	27.387522186663315	29.107353863887734	21.717496033184915
30-31	22.140912045772097	27.24001913743904	28.940603146513418	21.67846567027545
32-33	21.83243559875526	27.408079694764677	28.906048928902095	21.853435777577975
34-35	22.03303702157916	27.430121184211455	28.879075463394084	21.6577663308153
36-37	21.957025626346482	27.41141214667431	28.81119144250149	21.820370784477717
38-39	21.83110358754649	27.470247753579812	28.928324315144504	21.770324343729193
40-41	22.13149413799974	27.34519016579746	28.719041459380307	21.80427423682249
42-43	22.031310835908496	27.459595367186928	28.52892910389069	21.980164693013887
44-45	22.13590696837591	27.542324850605354	28.32243723765951	21.99933094335923
46-47	22.193104834893237	27.57350570265757	28.003216952312	22.230172510137198
48-49	22.102409806628327	27.722890757307205	27.96446759422897	22.210231841835498
50-51	22.156826573446814	27.854184708283658	27.839020569441498	22.14996814882803
52-53	22.311803178066505	27.76062672087432	27.73878605363868	22.18878404742049
54-55	22.29137164009998	27.680621593412962	27.742335570499034	22.28567119598802
56-57	22.287703431472554	27.822125743685177	27.691583267415776	22.198587557426492
58-59	22.240422856601413	27.8764571667636	27.749843553566016	22.13327642306897
60-61	22.327134679494343	27.874924249651055	27.685754824781455	22.112186246073147
62-63	22.252086725985933	27.94329286495976	27.64771256167431	22.156907847380005
64-65	22.245347229548212	27.85686496793031	27.626195392078557	22.271592410442924
66-67	22.314920189096142	27.850648702042868	27.527837510319102	22.306593598541888
68-69	22.272448072493376	27.823564087785037	27.606057063514484	22.29793077620711
70-71	22.29572868443867	27.621934106916957	27.655674249810698	22.42666295883367
72-73	22.33136879799548	27.67512470589636	27.555934996734933	22.43757149937323
74-75	22.205982210975012	27.754748316442353	27.560670931844534	22.4785985407381
76-77	22.340315803579664	27.69414889142722	27.497170483927015	22.468364821066103
78-79	22.332984965697754	27.690389184122687	27.460720122469912	22.515905727709647
80-81	22.310581429600653	27.724151634026338	27.496885036621656	22.468381899751357
82-83	22.33617485290211	27.681405290524786	27.572866755574733	22.40955310099837
84-85	22.493257493265574	27.618011603020314	27.498214659767044	22.39051624394707
86-87	22.330045373090414	27.612512805362176	27.59106761708327	22.466374204464138
88-89	22.36511143348311	27.57731221710683	27.599117144349655	22.458459205060404
90-91	22.43351240277825	27.57013299892191	27.581885798655126	22.414468799644716
92-93	22.458538556862347	27.493220058069177	27.62633499049664	22.421906394571835
94-95	22.362442130601046	27.524365381841164	27.656382265361763	22.456810222196026
96-97	22.42189595666307	27.663069790847665	27.69410712562106	22.220927126868204
98-99	22.601149261675353	28.375538891667784	26.80533882976351	22.21797301689335
100	20.63662007623207	28.166152028266822	27.982997230093627	23.21423066540748
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1609.0
1	1496.5
2	2318.0
3	3523.5
4	4915.0
5	8121.5
6	10846.0
7	12801.0
8	14321.0
9	14315.5
10	13141.0
11	11819.5
12	12658.0
13	16848.0
14	26649.0
15	39950.0
16	50766.5
17	57295.0
18	58475.0
19	54564.0
20	48225.5
21	41663.5
22	37201.0
23	37381.5
24	42466.0
25	52574.0
26	67232.0
27	83570.5
28	101868.5
29	122795.5
30	143069.5
31	163676.0
32	186310.0
33	209217.0
34	232317.5
35	252992.0
36	275184.0
37	295657.5
38	309592.5
39	321996.5
40	334717.5
41	346891.0
42	354475.5
43	361200.0
44	367135.5
45	373897.5
46	379376.5
47	380403.0
48	380791.0
49	377198.5
50	370887.5
51	360344.0
52	345029.0
53	327613.5
54	308412.0
55	286990.5
56	263335.5
57	237184.5
58	209573.0
59	180506.0
60	149646.5
61	120000.5
62	93596.0
63	70953.5
64	51575.5
65	36898.5
66	26699.0
67	19790.0
68	14627.5
69	10087.0
70	6739.0
71	4725.0
72	3185.5
73	1992.5
74	1239.5
75	790.5
76	508.0
77	306.5
78	196.5
79	128.5
80	90.0
81	62.0
82	44.5
83	31.0
84	20.0
85	15.5
86	11.0
87	7.5
88	6.0
89	4.5
90	3.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	5.2086019018688983E-5
7	0.0
8	0.0
9	0.0
10-11	0.005651333063527755
12-13	1.5625805705606694E-5
14-15	0.004005414862537183
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	127.0
20-21	546.0
22-23	1208.0
24-25	2796.0
26-27	3241.0
28-29	4996.0
30-31	9932.0
32-33	12645.0
34-35	17756.0
36-37	23560.0
38-39	29012.0
40-41	33439.0
42-43	43124.0
44-45	53584.0
46-47	51093.0
48-49	44093.0
50-51	37856.0
52-53	37241.0
54-55	40607.0
56-57	44224.0
58-59	44935.0
60-61	46346.0
62-63	47144.0
64-65	50418.0
66-67	56504.0
68-69	56550.0
70-71	56871.0
72-73	56496.0
74-75	59738.0
76-77	59641.0
78-79	66107.0
80-81	69120.0
82-83	68936.0
84-85	71694.0
86-87	77104.0
88-89	84991.0
90-91	90858.0
92-93	96148.0
94-95	112904.0
96-97	334659.0
98-99	40359.0
100-101	7460902.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.33051673998203
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.17001934247489	75.197538088651
2	7.622113777323397	12.85553586985237
3	1.787124418551003	4.521273770851381
4	0.6548778592356213	2.2090475308365263
5	0.28452615480981225	1.1997118830575797
6	0.16890631144984822	0.8546373915126027
7	0.08827506518519554	0.5210997303626197
8	0.05680001705242456	0.3831979831096603
9	0.03830586808176496	0.29073182845579415
>10	0.1250118647600085	1.6648611263523931
>50	0.003303901804702601	0.1831736749696177
>100	7.144113523532192E-4	0.1030694831058924
>500	2.1007919041539188E-5	0.01612163888248284
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0417203803737797E-5
2	0.0	1.0417203803737797E-5	0.0	0.0	2.0834407607475595E-5
3	0.0	1.0417203803737797E-5	0.0	0.0	2.0834407607475595E-5
4	0.0	1.1458924184111576E-4	0.0	0.0	2.0834407607475595E-5
5	0.0	1.1458924184111576E-4	0.0	0.0	2.0834407607475595E-5
6	0.0	1.1458924184111576E-4	0.0	0.0	2.0834407607475595E-5
7	0.0	1.1458924184111576E-4	0.0	1.0417203803737797E-5	2.0834407607475595E-5
8	0.0	1.2500644564485355E-4	0.0	3.125161141121339E-5	2.0834407607475595E-5
9	0.0	2.0834407607475593E-4	0.0	3.125161141121339E-5	2.0834407607475595E-5
10-11	0.0	2.1876127987849374E-4	0.0	4.166881521495119E-5	2.0834407607475595E-5
12-13	0.0	2.1876127987849374E-4	0.0	4.166881521495119E-5	2.0834407607475595E-5
14-15	0.0	2.864731046027894E-4	0.0	5.729462092055789E-5	5.2086019018688983E-5
16-17	2.0834407607475595E-5	2.9168170650465834E-4	0.0	7.292042662616458E-5	6.250322282242678E-5
18-19	2.0834407607475595E-5	3.07307512210265E-4	0.0	7.292042662616458E-5	8.333763042990238E-5
20-21	2.0834407607475595E-5	3.229333179158717E-4	0.0	7.812902852803348E-5	1.2500644564485355E-4
22-23	2.0834407607475595E-5	4.1668815214951187E-4	0.0	1.0938063993924687E-4	1.3021504754672246E-4
24-25	2.0834407607475595E-5	4.5314836546259417E-4	0.0	1.3021504754672246E-4	1.4584085325232917E-4
26-27	2.0834407607475595E-5	4.6877417116820085E-4	0.0	1.5625805705606695E-4	1.5104945515419807E-4
28-29	2.0834407607475595E-5	4.6877417116820085E-4	0.0	2.2917848368223152E-4	1.5625805705606695E-4
30-31	2.0834407607475595E-5	5.312773939906277E-4	0.0	4.4273116165885636E-4	1.5625805705606695E-4
32-33	2.0834407607475595E-5	5.416945977943654E-4	0.0	8.073332947896794E-4	1.7709246466354254E-4
34-35	2.0834407607475595E-5	6.198236263223989E-4	0.0	0.001437574124915816	1.7709246466354254E-4
36-37	2.0834407607475595E-5	7.031612567523014E-4	0.0	0.002307410642527922	1.7709246466354254E-4
38-39	2.0834407607475595E-5	7.396214700653836E-4	0.0	0.003958537445420363	1.8230106656541144E-4
40-41	3.125161141121339E-5	7.917074890840726E-4	0.0	0.005844051333896904	1.8750966846728035E-4
42-43	3.125161141121339E-5	8.22959100495286E-4	0.0	0.007656644795747281	1.8750966846728035E-4
44-45	4.6877417116820086E-5	9.010881290233195E-4	0.0	0.00959945330514438	1.8750966846728035E-4
46-47	5.2086019018688983E-5	9.479655461401396E-4	0.0	0.011984992976200336	1.8750966846728035E-4
48-49	5.2086019018688983E-5	9.635913518457463E-4	0.0	0.014297612220630126	2.1876127987849374E-4
50-51	7.812902852803348E-5	0.0011198494089018133	0.0	0.01668315189168608	2.2917848368223152E-4
52-53	8.333763042990238E-5	0.0011250580108036821	0.0	0.019152029193171938	2.395956874859693E-4
54-55	8.333763042990238E-5	0.0011406838165092888	0.0	0.021631323698461536	2.5522149319157604E-4
56-57	8.333763042990238E-5	0.0011771440298223711	0.0	0.024115826805653003	2.604300950934449E-4
58-59	8.333763042990238E-5	0.00118235263172424	0.0	0.026975349249779026	2.604300950934449E-4
60-61	8.854623233177128E-5	0.0012136042431354532	0.0	0.029928626528138692	2.708472988971827E-4
62-63	1.0417203803737797E-4	0.0012188128450373223	0.0	0.032642308119012385	3.020989103083961E-4
64-65	1.0417203803737797E-4	0.001333402086878438	0.0	0.03547578755362907	3.020989103083961E-4
66-67	1.1458924184111576E-4	0.0013542364944859136	0.0	0.03869470352898405	3.125161141121339E-4
68-69	1.1458924184111576E-4	0.0013802795039952582	0.0	0.041627146399736234	3.125161141121339E-4
70-71	1.1458924184111576E-4	0.0014063225135046026	0.0	0.044616883891408984	3.125161141121339E-4
72-73	1.3542364944859136E-4	0.0014219483192102092	0.0	0.04714826441571727	3.125161141121339E-4
74-75	1.3542364944859136E-4	0.0014636171344251606	0.0	0.04928899979738539	3.125161141121339E-4
76-77	1.3542364944859136E-4	0.0015157031534438494	0.0	0.05133598034481986	3.125161141121339E-4
78-79	1.3542364944859136E-4	0.0015313289591494562	0.0	0.05268500823740391	3.125161141121339E-4
80-81	1.3542364944859136E-4	0.0015313289591494562	0.0	0.05314857380667024	3.177247160140028E-4
82-83	1.3542364944859136E-4	0.0015313289591494562	0.0	0.05358088776452535	3.229333179158717E-4
84-85	1.3542364944859136E-4	0.0015469547648550629	0.0	0.05371110281207209	3.229333179158717E-4
86-87	1.4063225135046027E-4	0.0015729977743644073	0.0	0.053778814636796374	3.2814191981774064E-4
88	1.4584085325232917E-4	0.0016563354047943097	0.0	0.053794440442501985	3.333505217196095E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13140	0.0	53.660114	1
GTATCAA	24050	0.0	38.46588	1
TCAACGC	29265	0.0	31.143475	4
ATCAACG	29645	0.0	30.729202	3
CAACGCA	30045	0.0	30.338116	5
TATCAAC	30675	0.0	29.870209	2
AACGCAG	30745	0.0	29.733606	6
ACGCAGA	34880	0.0	26.20857	7
CGCAGAG	35385	0.0	25.83534	8
GTACATG	29725	0.0	22.457254	1
TACATGG	29200	0.0	22.104538	2
GCAGAGT	41455	0.0	21.91524	9
ACATGGG	29535	0.0	21.061037	3
GAGTACT	25490	0.0	20.661362	12-13
GTGGTAT	6375	0.0	20.09703	1
CAGAGTA	40535	0.0	19.730919	10-11
TGGTATC	5970	0.0	19.684364	2
AGAGTAC	37590	0.0	19.519356	10-11
CATGGGG	20930	0.0	18.68058	4
GTACTTT	28565	0.0	18.37541	14-15
>>END_MODULE