FastQCFastQC Report
Fri 27 May 2016
SRR2139216_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139216_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9937001
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130300.051.518651
GTATCAA238350.038.3162961
TCAACGC285900.031.3425544
ATCAACG288900.031.0932123
CAACGCA294800.030.42995
TATCAAC301350.030.0133932
AACGCAG300150.030.0049956
ACGCAGA345950.026.0199247
CGCAGAG373350.024.134318
GTACATG312550.022.2675061
TACATGG306750.021.9557232
GCAGAGT415750.021.5575479
ACATGGG311850.021.101973
GAGTACT243350.020.77646412-13
GTGGTAT67000.019.9989761
TGGTATC64000.019.559032
CAGAGTA398750.019.3753910-11
AGAGTAC369650.019.27871510-11
CATGGGG231850.018.7638194
CTTATAC53850.018.7438471
Produced by FastQC (version 0.11.5)