FastQCFastQC Report
Fri 27 May 2016
SRR2139215_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139215_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10059668
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA164850.056.829711
GTATCAA309550.039.92881
TCAACGC389100.031.31624
ATCAACG392850.031.0392273
TATCAAC397950.030.740352
CAACGCA399550.030.5003285
AACGCAG406800.030.0105116
ACGCAGA461850.026.4428967
GTGGTAT68700.026.2928831
TGGTATC66800.025.7312552
CGCAGAG504100.024.2526748
GTACATG397050.023.3856621
TACATGG394450.023.1295532
GCAGAGT536800.022.6385619
ACATGGG403850.022.0930143
GAGTACT317900.021.6361312-13
CAGAGTA521500.020.43383610-11
TATATAC119850.020.0345333
CATGGGG292800.019.8071354
AGAGTAC494450.019.53898210-11
Produced by FastQC (version 0.11.5)