##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139215_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10059668
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6009658569249	33.0	33.0	33.0	33.0	33.0
2	32.605534099137266	33.0	33.0	33.0	33.0	33.0
3	32.59998988038174	33.0	33.0	33.0	33.0	33.0
4	36.50574849985109	37.0	37.0	37.0	37.0	37.0
5	36.53134457320063	37.0	37.0	37.0	37.0	37.0
6	36.52571446691879	37.0	37.0	37.0	37.0	37.0
7	36.484218763482055	37.0	37.0	37.0	37.0	37.0
8	36.48596524259051	37.0	37.0	37.0	37.0	37.0
9	36.5288506539182	37.0	37.0	37.0	37.0	37.0
10-11	36.45097810384995	37.0	37.0	37.0	37.0	37.0
12-13	36.44568513593093	37.0	37.0	37.0	37.0	37.0
14-15	38.47507561879776	40.0	37.0	40.0	37.0	40.0
16-17	38.41415541745513	40.0	37.0	40.0	37.0	40.0
18-19	38.442090733014254	40.0	37.0	40.0	37.0	40.0
20-21	38.42561515928054	40.0	37.0	40.0	37.0	40.0
22-23	38.433624042047	40.0	37.0	40.0	37.0	40.0
24-25	38.41963467361197	40.0	37.0	40.0	37.0	40.0
26-27	38.311405868881195	40.0	37.0	40.0	37.0	40.0
28-29	38.23950432292314	40.0	37.0	40.0	37.0	40.0
30-31	38.171662863722574	40.0	37.0	40.0	37.0	40.0
32-33	38.09446657068317	40.0	37.0	40.0	33.0	40.0
34-35	38.04998868792423	40.0	37.0	40.0	33.0	40.0
36-37	37.982876438112356	40.0	37.0	40.0	33.0	40.0
38-39	37.919564255678466	40.0	37.0	40.0	33.0	40.0
40-41	37.87392283109665	40.0	37.0	40.0	33.0	40.0
42-43	37.810302344535486	40.0	37.0	40.0	33.0	40.0
44-45	37.73316464446665	40.0	37.0	40.0	33.0	40.0
46-47	37.67375661980684	40.0	37.0	40.0	33.0	40.0
48-49	37.63017765438548	40.0	37.0	40.0	33.0	40.0
50-51	37.536000762786344	40.0	37.0	40.0	33.0	40.0
52-53	37.420163609687364	40.0	37.0	40.0	33.0	40.0
54-55	37.215585780144416	40.0	37.0	40.0	33.0	40.0
56-57	37.12441114491641	37.0	37.0	40.0	33.0	40.0
58-59	36.979250774146664	37.0	37.0	40.0	33.0	40.0
60-61	36.83232940939436	37.0	37.0	40.0	33.0	40.0
62-63	36.66507071946019	37.0	37.0	40.0	33.0	40.0
64-65	36.46772076988223	37.0	37.0	40.0	33.0	40.0
66-67	36.24955454061258	37.0	37.0	40.0	33.0	40.0
68-69	36.04849721452334	37.0	37.0	38.5	33.0	40.0
70-71	35.78381365017776	37.0	33.0	37.0	33.0	40.0
72-73	35.57775742221291	37.0	33.0	37.0	33.0	40.0
74-75	35.40501262956843	37.0	33.0	37.0	33.0	40.0
76-77	33.74054398026588	35.0	33.0	37.0	27.0	37.0
78-79	34.90468070329101	37.0	33.0	37.0	33.0	37.0
80-81	34.991177734301246	37.0	33.0	37.0	33.0	37.0
82-83	34.87769708102738	37.0	33.0	37.0	33.0	37.0
84-85	34.71344335448495	37.0	33.0	37.0	33.0	37.0
86-87	34.639879352574084	37.0	33.0	37.0	33.0	37.0
88-89	34.54677810580948	37.0	33.0	37.0	33.0	37.0
90-91	34.43869595203248	37.0	33.0	37.0	33.0	37.0
92-93	34.4002662832698	37.0	33.0	37.0	33.0	37.0
94-95	34.43178999970266	37.0	33.0	37.0	33.0	37.0
96-97	34.4397555503702	37.0	33.0	37.0	33.0	37.0
98-99	34.43987926750276	37.0	33.0	37.0	33.0	37.0
100	34.452571358656186	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	4.0
18	5.0
19	27.0
20	88.0
21	452.0
22	1624.0
23	4558.0
24	10914.0
25	21253.0
26	36816.0
27	58575.0
28	85318.0
29	118886.0
30	160148.0
31	210764.0
32	277583.0
33	369222.0
34	506334.0
35	739568.0
36	1327359.0
37	3051865.0
38	3015513.0
39	62791.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.553889452415326	19.637984076611673	14.191422619513885	23.616703851459114
2	15.087436285173625	20.91842394798715	41.21540591598053	22.778733850858696
3	18.48632579126866	26.071576119609514	29.997272275784848	25.44482581333698
4	12.550980807716517	18.008646010981675	38.01236780378836	31.42800537751345
5	13.727898375970259	37.43497300308519	34.934065418461124	13.90306320248342
6	30.283767327889144	37.24590815783849	18.54871690366961	13.921607610602754
7	26.932817265937604	32.48225488157263	22.615239389610075	17.96968846287969
8	25.175204589256822	36.04272029653464	20.43671818990448	18.345356924304063
9	26.101795804791966	16.411913395153796	20.804841670719153	36.68144912933508
10-11	24.85288471613515	26.56204549941655	27.884025219706142	20.70104456474216
12-13	25.711527031660193	24.257169745280834	28.117128529204795	21.91417469385418
14-15	22.71383190289132	25.192770431578115	26.32197545640851	25.771422209122058
16-17	22.017858839874236	28.46477637234151	26.768999732396736	22.748365055387517
18-19	22.02592570649449	27.251003711056864	28.92594467332322	21.79712590912543
20-21	23.18691061401393	26.25552141431711	28.92091059701483	21.636657374654135
22-23	22.894563394831902	26.3112585740989	28.6585808693484	22.135597161720803
24-25	22.522244943549058	26.4965054793787	28.948688477715134	22.03256109935711
26-27	22.373900496503357	26.572392021946477	28.925768303970052	22.127939177580114
28-29	22.380813668814444	26.587600226746716	28.882758889737243	22.148827214701594
30-31	22.70761202163096	26.376464672995237	28.74647567612685	22.169447629246957
32-33	22.641935872860024	26.40851690541452	28.63121034742807	22.31833687429739
34-35	22.720407731771694	26.558635572992355	28.576391053833994	22.144565641401957
36-37	22.755081020820416	26.511104073736636	28.386839277224134	22.346975628218814
38-39	22.439458656178466	26.804986016031545	28.488026179910214	22.267529147879774
40-41	22.67712637826803	26.58033675565179	28.425692319789608	22.316844546290575
42-43	22.53999832565384	26.757014466472434	28.052671565244058	22.650315642629664
44-45	22.52847134000624	26.89225861180928	27.863766794786994	22.71550325339749
46-47	22.78381422632571	26.858531313666166	27.526232780511094	22.831421679497026
48-49	22.929858450396445	27.153394916012356	27.19919991520773	22.71754671838347
50-51	22.888717470446355	27.16648608779826	27.182829212268683	22.7619672294867
52-53	23.146780873563824	27.129510205401946	27.062912508948543	22.660796412085684
54-55	23.18175090771946	27.06924257369004	26.944272165105637	22.80473435348486
56-57	23.166606884092293	27.370251540129114	26.708530476421117	22.754611099357476
58-59	22.906275498720348	27.31674163535852	27.033310209718138	22.743672656202992
60-61	23.026962760406466	27.322582961949788	26.870690439909282	22.77976383773446
62-63	23.0306794041758	27.351251617605993	26.958770874871295	22.659298103346913
64-65	23.136027405320522	27.19758897093475	26.882121170081085	22.784262453663644
66-67	23.20101936375955	27.275398529298123	26.6543059336844	22.86927617325793
68-69	23.12815791253922	27.211526694110134	26.771747794783927	22.888567598566716
70-71	23.116843058988245	27.060245847946923	26.78400656194434	23.038904531120494
72-73	23.103445000617384	27.138462177657292	26.656603008110746	23.101489813614577
74-75	22.99735740763754	27.13471066246208	26.696388020673194	23.171543909227186
76-77	23.100121290356192	27.168763524511807	26.60865048931945	23.122464695812546
78-79	22.963710278863	27.160323851902273	26.70728689508963	23.16867897414509
80-81	23.038359533703545	27.045242572199484	26.803219734235117	23.113178159861853
82-83	23.244685621847346	26.904593639984647	26.95655180952349	22.89416892864452
84-85	23.19955087340641	26.923959523294272	26.864446282094047	23.012043321205276
86-87	23.018200770423178	27.06949029501944	26.8146813940376	23.097627540519788
88-89	23.237157167966895	26.851137637048893	26.706080610567952	23.205624584416263
90-91	23.223899753009615	26.85794768519597	26.753777123648547	23.16437543814586
92-93	23.245567815229137	26.855970611240053	26.917888968603755	22.98057260492705
94-95	23.006013741937327	27.061610206531732	26.942185183330103	22.990190868200834
96-97	23.025083967275016	27.078882405681536	26.99711838113522	22.89891524590823
98-99	23.33068645182215	27.846892007889295	26.049178290000146	22.77324325028841
100	21.21580731057855	27.71844379190216	27.308973508888258	23.75677538863103
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2302.0
1	2060.0
2	3096.5
3	4606.0
4	6373.0
5	10967.5
6	14881.0
7	17372.5
8	18977.0
9	18495.0
10	16549.0
11	14539.5
12	15653.0
13	21470.5
14	35450.0
15	54908.0
16	71457.0
17	81312.5
18	82358.0
19	75570.0
20	64878.5
21	53197.0
22	43737.5
23	40454.5
24	43710.0
25	52119.0
26	64783.5
27	79045.5
28	94806.5
29	112900.5
30	130266.0
31	148044.0
32	167815.0
33	188287.0
34	207880.5
35	226124.0
36	246787.5
37	265617.5
38	279069.0
39	293327.0
40	308226.0
41	320718.5
42	328960.0
43	336874.5
44	347275.5
45	363857.5
46	385087.5
47	405304.5
48	416470.0
49	418297.5
50	417966.0
51	415304.0
52	394012.5
53	362081.0
54	342727.5
55	327252.5
56	308219.0
57	280637.0
58	250910.5
59	221445.5
60	189744.5
61	164230.5
62	136738.5
63	112614.5
64	95501.0
65	75495.0
66	60387.0
67	44293.0
68	27575.0
69	19676.0
70	14041.0
71	8360.0
72	5563.5
73	4088.5
74	3236.0
75	2430.0
76	1505.5
77	730.5
78	536.5
79	453.0
80	301.5
81	189.5
82	156.5
83	108.0
84	63.0
85	43.0
86	34.0
87	29.0
88	28.0
89	22.5
90	12.5
91	9.0
92	6.5
93	3.0
94	1.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.7893234647505267E-4
7	0.0
8	0.0
9	0.0
10-11	0.005467377253404387
12-13	9.940685915280704E-6
14-15	0.0042049101421637375
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	189.0
20-21	721.0
22-23	1533.0
24-25	3235.0
26-27	4254.0
28-29	6930.0
30-31	12959.0
32-33	16683.0
34-35	23012.0
36-37	29377.0
38-39	37226.0
40-41	43550.0
42-43	58918.0
44-45	71790.0
46-47	68662.0
48-49	56357.0
50-51	45546.0
52-53	43659.0
54-55	47331.0
56-57	51588.0
58-59	52569.0
60-61	52599.0
62-63	54224.0
64-65	59419.0
66-67	65567.0
68-69	66935.0
70-71	67088.0
72-73	64948.0
74-75	68083.0
76-77	67048.0
78-79	74400.0
80-81	77731.0
82-83	76350.0
84-85	79784.0
86-87	85646.0
88-89	94259.0
90-91	100235.0
92-93	105253.0
94-95	122962.0
96-97	352486.0
98-99	47522.0
100-101	7601040.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.11396606995159
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.87863670653702	73.87055995570672
2	7.627180822059156	12.678504961080202
3	1.8955953683145517	4.726513473733591
4	0.6954239675498342	2.3119777617267214
5	0.33247191948713467	1.3816529917732694
6	0.18070047961843788	0.9011240119098494
7	0.10776912806847785	0.6269983757570214
8	0.06753455764488078	0.44904519461166487
9	0.04733375367880566	0.354068639750133
>10	0.16104461265441786	2.1889448739018524
>50	0.0047490395054022696	0.2675485723961269
>100	0.0014693023268455428	0.1825786753116258
>500	6.775723748484597E-5	0.03692421303065494
>1k	2.2585317634538153E-5	0.023558299310628843
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.958480140696492E-5	0.0	0.0	0.0
3	0.0	6.958480140696492E-5	0.0	0.0	0.0
4	0.0	1.1928823098336843E-4	0.0	0.0	0.0
5	0.0	1.1928823098336843E-4	0.0	0.0	0.0
6	4.970342957640352E-5	1.1928823098336843E-4	0.0	0.0	0.0
7	4.970342957640352E-5	1.1928823098336843E-4	0.0	0.0	0.0
8	4.970342957640352E-5	1.1928823098336843E-4	0.0	9.940685915280703E-6	0.0
9	4.970342957640352E-5	3.379833211195439E-4	0.0	9.940685915280703E-6	0.0
10-11	4.970342957640352E-5	3.379833211195439E-4	0.0	9.940685915280703E-6	0.0
12-13	4.970342957640352E-5	3.379833211195439E-4	0.0	9.940685915280703E-6	0.0
14-15	5.9644115491684216E-5	4.473308661876316E-4	0.0	9.940685915280703E-6	0.0
16-17	8.946617323752633E-5	4.622418950605527E-4	0.0	9.940685915280703E-6	0.0
18-19	8.946617323752633E-5	4.721825809758334E-4	0.0	1.4911028872921054E-5	4.970342957640351E-6
20-21	8.946617323752633E-5	4.970342957640352E-4	0.0	2.4851714788201755E-5	9.940685915280703E-6
22-23	8.946617323752633E-5	5.367970394251579E-4	0.0	3.479240070348246E-5	9.940685915280703E-6
24-25	8.946617323752633E-5	5.815301260439212E-4	0.0	5.4673772534043867E-5	9.940685915280703E-6
26-27	8.946617323752633E-5	6.063818408321229E-4	0.0	9.443651619516668E-5	9.940685915280703E-6
28-29	9.940685915280703E-5	6.063818408321229E-4	0.0	2.6342817675493866E-4	1.9881371830561405E-5
30-31	9.940685915280703E-5	7.256700718154914E-4	0.0	5.566784112557194E-4	2.9822057745842108E-5
32-33	1.0437720211044739E-4	7.256700718154914E-4	0.0	0.001824115865454009	2.9822057745842108E-5
34-35	1.0934754506808773E-4	8.101659020953773E-4	0.0	0.0032903670379579126	2.9822057745842108E-5
36-37	1.0934754506808773E-4	8.946617323752632E-4	0.0	0.005427614509743264	2.9822057745842108E-5
38-39	1.0934754506808773E-4	9.393948189940264E-4	0.0	0.008141421764614895	2.9822057745842108E-5
40-41	1.0934754506808773E-4	0.0010139499633586317	0.0	0.010666355987096195	2.9822057745842108E-5
42-43	1.0934754506808773E-4	0.001058683049977395	0.0	0.013245963982111537	2.9822057745842108E-5
44-45	1.0934754506808773E-4	0.0011630602520878424	0.0	0.01642698347500136	2.9822057745842108E-5
46-47	1.0934754506808773E-4	0.0012227043675795265	0.0	0.019836638743942644	2.9822057745842108E-5
48-49	1.0934754506808773E-4	0.0012276747105371669	0.0	0.023519662875554147	2.9822057745842108E-5
50-51	1.0934754506808773E-4	0.0014066070570122194	0.0	0.027083398776182273	2.9822057745842108E-5
52-53	1.0934754506808773E-4	0.0014115773999698598	0.0	0.030343943756394345	2.9822057745842108E-5
54-55	1.0934754506808773E-4	0.0014115773999698598	0.0	0.03361939976547934	2.9822057745842108E-5
56-57	1.0934754506808773E-4	0.0014215180858851404	0.0	0.03660160554006355	3.976274366112281E-5
58-59	1.0934754506808773E-4	0.0014264884288427808	0.0	0.039742862289292254	4.970342957640352E-5
60-61	1.1431788802572808E-4	0.0014911028872921055	0.0	0.043038199670207805	4.970342957640352E-5
62-63	1.2922891689864913E-4	0.0015557173457414301	0.0	0.046487617682810206	4.970342957640352E-5
64-65	1.3419925985628947E-4	0.0017793827788352458	0.0	0.04958911168837779	5.9644115491684216E-5
66-67	1.3916960281392984E-4	0.0017992641506658072	0.0	0.05315284758900592	5.9644115491684216E-5
68-69	1.3916960281392984E-4	0.0018738192950304125	0.0	0.05687563446427854	5.9644115491684216E-5
70-71	1.4413994577157021E-4	0.0019135820386915353	0.0	0.06034990419166915	5.9644115491684216E-5
72-73	1.4911028872921056E-4	0.001923522724606816	0.0	0.06310844453315954	5.9644115491684216E-5
74-75	1.4911028872921056E-4	0.0019583151253102986	0.0	0.0653699505788859	6.461445844932457E-5
76-77	1.4911028872921056E-4	0.002042810955590184	0.0	0.0676612786823581	6.958480140696492E-5
78-79	1.4911028872921056E-4	0.002082573699251307	0.0	0.06909770779711616	7.455514436460528E-5
80-81	1.4911028872921056E-4	0.002092514385166588	0.0	0.06964941586541425	7.952548732224562E-5
82-83	1.4911028872921056E-4	0.0021024550710818683	0.0	0.07009177638864425	7.952548732224562E-5
84-85	1.4911028872921056E-4	0.0021223364429124305	0.0	0.07026573839216166	7.952548732224562E-5
86-87	1.4911028872921056E-4	0.0021670695295311934	0.0	0.07034029353652625	8.449583027988598E-5
88	1.5905097464449124E-4	0.002246595016853439	0.0	0.07035023422244154	8.946617323752633E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16485	0.0	56.82971	1
GTATCAA	30955	0.0	39.9288	1
TCAACGC	38910	0.0	31.3162	4
ATCAACG	39285	0.0	31.039227	3
TATCAAC	39795	0.0	30.74035	2
CAACGCA	39955	0.0	30.500328	5
AACGCAG	40680	0.0	30.010511	6
ACGCAGA	46185	0.0	26.442896	7
GTGGTAT	6870	0.0	26.292883	1
TGGTATC	6680	0.0	25.731255	2
CGCAGAG	50410	0.0	24.252674	8
GTACATG	39705	0.0	23.385662	1
TACATGG	39445	0.0	23.129553	2
GCAGAGT	53680	0.0	22.638561	9
ACATGGG	40385	0.0	22.093014	3
GAGTACT	31790	0.0	21.63613	12-13
CAGAGTA	52150	0.0	20.433836	10-11
TATATAC	11985	0.0	20.034533	3
CATGGGG	29280	0.0	19.807135	4
AGAGTAC	49445	0.0	19.538982	10-11
>>END_MODULE