FastQCFastQC Report
Fri 27 May 2016
SRR2139214_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139214_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7907362
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108000.049.8978041
GTATCAA200350.038.2910161
TCAACGC238450.031.2908544
ATCAACG242950.030.747343
CAACGCA243900.030.5412375
AACGCAG249850.029.971766
TATCAAC251750.029.951012
ACGCAGA288000.026.0015417
CGCAGAG289950.025.8273268
GCAGAGT339400.022.0136629
GTACATG225500.022.0132121
GTGGTAT55700.021.7883661
TACATGG225400.021.3620952
ACATGGG227500.020.3753173
GAGTACT215150.020.21247512-13
TGGTATC54500.020.0171812
AGAGTAC303550.019.75578510-11
CAGAGTA330600.019.3057810-11
GTACTTT238850.018.21639814-15
AGTACTT235000.017.56359112-13
Produced by FastQC (version 0.11.5)