FastQCFastQC Report
Fri 27 May 2016
SRR2139213_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139213_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9422098
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA180900.053.0856131
GTATCAA342700.039.0574071
TCAACGC431700.030.5231234
ATCAACG434100.030.4227313
TATCAAC441600.030.1273522
CAACGCA440950.029.8758875
AACGCAG447100.029.4839446
ACGCAGA505850.026.0679057
CGCAGAG511000.025.7807858
CTTATAC110200.024.3307061
TGGTATC72900.024.0146712
GTGGTAT77950.023.3855781
GCAGAGT572850.022.9024039
GTACATG358550.021.9718671
TACATGG355800.021.5684412
TATACAC146950.021.513463
GAGTACT373900.021.10572412-13
ACATGGG358200.020.7714843
CAGAGTA562350.019.95176710-11
AGAGTAC537100.019.5920410-11
Produced by FastQC (version 0.11.5)