##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139213_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9422098
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63253927097765	33.0	33.0	33.0	33.0	33.0
2	32.6343577619337	33.0	33.0	33.0	33.0	33.0
3	32.627064587950585	33.0	33.0	33.0	33.0	33.0
4	36.53880070022621	37.0	37.0	37.0	37.0	37.0
5	36.56737278682518	37.0	37.0	37.0	37.0	37.0
6	36.556279185378884	37.0	37.0	37.0	37.0	37.0
7	36.52231318332711	37.0	37.0	37.0	37.0	37.0
8	36.52484648323547	37.0	37.0	37.0	37.0	37.0
9	36.55664810533705	37.0	37.0	37.0	37.0	37.0
10-11	36.48639262720469	37.0	37.0	37.0	37.0	37.0
12-13	36.48239468534503	37.0	37.0	37.0	37.0	37.0
14-15	38.536329276133614	40.0	37.0	40.0	37.0	40.0
16-17	38.4806458179484	40.0	37.0	40.0	37.0	40.0
18-19	38.52745943631662	40.0	37.0	40.0	37.0	40.0
20-21	38.52397777009952	40.0	37.0	40.0	37.0	40.0
22-23	38.530547980611075	40.0	37.0	40.0	37.0	40.0
24-25	38.5178924487079	40.0	37.0	40.0	37.0	40.0
26-27	38.405925236975776	40.0	37.0	40.0	37.0	40.0
28-29	38.330475466484025	40.0	37.0	40.0	37.0	40.0
30-31	38.25754504490487	40.0	37.0	40.0	37.0	40.0
32-33	38.181139113367195	40.0	37.0	40.0	37.0	40.0
34-35	38.144813071480456	40.0	37.0	40.0	37.0	40.0
36-37	38.079643072095635	40.0	37.0	40.0	33.0	40.0
38-39	38.025721237010515	40.0	37.0	40.0	33.0	40.0
40-41	38.001413297341145	40.0	37.0	40.0	33.0	40.0
42-43	37.951026420013314	40.0	37.0	40.0	33.0	40.0
44-45	37.885823368185186	40.0	37.0	40.0	33.0	40.0
46-47	37.84546019289275	40.0	37.0	40.0	33.0	40.0
48-49	37.83156781181795	40.0	37.0	40.0	33.0	40.0
50-51	37.75634279117779	40.0	37.0	40.0	33.0	40.0
52-53	37.663800212107816	40.0	37.0	40.0	33.0	40.0
54-55	37.48854481044686	40.0	37.0	40.0	33.0	40.0
56-57	37.419715249733294	40.0	37.0	40.0	33.0	40.0
58-59	37.319719707765046	40.0	37.0	40.0	33.0	40.0
60-61	37.19298417572591	37.0	37.0	40.0	33.0	40.0
62-63	37.04844213001809	37.0	37.0	40.0	33.0	40.0
64-65	36.881083960634584	37.0	37.0	40.0	33.0	40.0
66-67	36.69105911095186	37.0	37.0	40.0	33.0	40.0
68-69	36.50545665893371	37.0	37.0	40.0	33.0	40.0
70-71	36.25269593920606	37.0	37.0	40.0	33.0	40.0
72-73	36.04783957573022	37.0	37.0	37.0	33.0	40.0
74-75	35.86490175508867	37.0	37.0	37.0	33.0	40.0
76-77	34.21901697363532	35.0	33.0	37.0	30.0	37.0
78-79	35.30461280008816	37.0	33.0	37.0	33.0	37.0
80-81	35.35486942282604	37.0	33.0	37.0	33.0	37.0
82-83	35.22993979946098	37.0	33.0	37.0	33.0	37.0
84-85	35.06330943782295	37.0	33.0	37.0	33.0	37.0
86-87	34.98181626670821	37.0	33.0	37.0	33.0	37.0
88-89	34.882485201409736	37.0	33.0	37.0	33.0	37.0
90-91	34.77617304212462	37.0	33.0	37.0	33.0	37.0
92-93	34.75150106473785	37.0	33.0	37.0	33.0	37.0
94-95	34.78135023671	37.0	33.0	37.0	33.0	37.0
96-97	34.77311160712854	37.0	33.0	37.0	33.0	37.0
98-99	34.752811656583	37.0	33.0	37.0	33.0	37.0
100	34.73937315020146	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	3.0
17	5.0
18	3.0
19	17.0
20	69.0
21	389.0
22	1354.0
23	3923.0
24	8911.0
25	17065.0
26	29768.0
27	47081.0
28	69136.0
29	95857.0
30	129784.0
31	172556.0
32	228546.0
33	306615.0
34	420009.0
35	606171.0
36	1058463.0
37	2625134.0
38	3485403.0
39	115836.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.8217365177055	20.645157798188897	14.682642867862338	22.85046281624326
2	14.26955015751269	21.668210201167508	42.31620176313173	21.746037878188062
3	17.615758188887444	27.55231372036249	31.227217122980466	23.6047109677696
4	11.774277873144602	18.838458271183338	39.74883300937859	29.638430846293467
5	12.51366733820854	38.460139132494696	35.82001588181316	13.206177647483608
6	27.999561456296234	39.074679153063	19.352566425255365	13.573192965385402
7	24.907998197429066	33.82445183652303	23.72717838426219	17.540371581785713
8	23.898127572012093	36.81901843941763	21.76146968541401	17.52138430315626
9	25.25045908034495	16.900078942078505	21.700411097401023	36.14905088017552
10-11	23.396822618666214	27.410126779912908	29.15485842662951	20.038192174791373
12-13	24.700237924729603	25.171555484328195	29.399616168268267	20.728590422673932
14-15	21.87997554160268	26.38129074829598	27.48367520853161	24.255058501569728
16-17	20.781215606120846	29.613505399752793	28.049872756577145	21.555406237549217
18-19	20.72829214894602	28.190738410914427	30.577903137921087	20.503066302218464
20-21	21.678060491818414	27.159435010619028	30.622436123997932	20.540068373564626
22-23	21.347981969912667	27.368988553370976	30.463776012430355	20.819253464286
24-25	21.08980040490784	27.641726371594128	30.512155065847296	20.75631815765074
26-27	20.99689018208517	27.90403520013053	30.485488932719328	20.61358568506497
28-29	20.88780883637302	27.81500599292752	30.535537356092025	20.76164781460744
30-31	21.353499168257468	27.598981936713297	30.402247149286065	20.64527174574317
32-33	21.004535657093403	27.78889702365097	30.364084155760622	20.842483163495
34-35	21.085751168564745	27.708442273829103	30.440182064662284	20.765624492943864
36-37	21.002933958299906	27.879870319794474	30.226800826252457	20.89039489565316
38-39	20.923282154450863	27.70010948798431	30.366855395289804	21.00975296227502
40-41	21.119659093639523	27.613183056282192	30.280899208541275	20.986258641537013
42-43	21.212271941231172	27.726647904648296	29.99715643164057	21.063923722479956
44-45	21.170690869376255	28.01634022129866	29.6111248052441	21.20184410408099
46-47	21.476279809633077	27.91528348092846	29.054429096964128	21.554007612474336
48-49	21.36509534944299	28.15147965887721	28.932862359861446	21.550562631818355
50-51	21.33723648105484	28.32345270276429	28.80941180520261	21.529899010978255
52-53	21.43127882708574	28.329399604227117	28.823719511461686	21.415602057225456
54-55	21.483941956561313	28.261436751646414	28.634711048882465	21.619910242909803
56-57	21.601850941776082	28.376105084009783	28.41824346694529	21.60380050726884
58-59	21.344655047784048	28.60579650607471	28.650820925609427	21.398727520531818
60-61	21.634729593536917	28.74039417815391	28.338487410049822	21.286388818259347
62-63	21.41626613674116	28.8310111880772	28.322080571209852	21.430642103971792
64-65	21.441538538274017	28.707658160370592	28.304826474533627	21.54597682682176
66-67	21.559146465099595	28.55051960301035	28.215024127140794	21.675309804749265
68-69	21.552278129553457	28.465282935169274	28.298536998319683	21.683901936957582
70-71	21.571178022099787	28.262792855807255	28.39846791435121	21.76756120774175
72-73	21.592502845313895	28.289641496381286	28.31802126628223	21.799834392022593
74-75	21.539651901914556	28.34628631191734	28.261132451766635	21.852929334401466
76-77	21.70218614279977	28.267575482256863	28.178605368954667	21.851633005988703
78-79	21.711817711185198	28.233149706900495	28.113160529352815	21.94187205256149
80-81	21.68559085390148	28.289729385760698	28.137673681819926	21.887006078517896
82-83	21.716048479543943	28.229439393293887	28.250056349281653	21.804455777880516
84-85	21.871595868502077	28.155948561085793	28.14399299317134	21.828462577240796
86-87	21.71078687363968	28.189763037161914	28.264598302520767	21.834851786677635
88-89	21.75819473507178	28.14442277810126	28.218542562144183	21.878839924682776
90-91	21.931491888654847	28.027720325180738	28.244615946456296	21.79617183970812
92-93	21.86103943856958	28.001760176944845	28.27092817726513	21.866272207220447
94-95	21.74114488197719	28.00297502794708	28.35308241352603	21.9027976765497
96-97	21.82845981700796	28.08248621268922	28.39928273365036	21.68977123665246
98-99	22.86373833603981	29.96300525896437	28.62757841806083	18.545677986934994
100	21.797558311292626	30.88495170787376	22.63903464913243	24.678455331701183
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3756.0
1	3346.5
2	4727.5
3	6868.0
4	9186.5
5	14721.5
6	19612.5
7	24105.5
8	28226.5
9	28221.5
10	24262.0
11	20479.5
12	21435.5
13	28149.5
14	44267.0
15	65847.0
16	82858.5
17	91662.0
18	91618.0
19	84625.5
20	74377.5
21	64533.0
22	58560.0
23	59083.0
24	66610.0
25	80046.0
26	98014.5
27	118755.5
28	140307.5
29	162896.0
30	184633.5
31	204918.5
32	226633.0
33	247104.0
34	265991.0
35	283486.0
36	301695.0
37	317911.0
38	326805.0
39	333365.0
40	340887.5
41	347757.0
42	352252.5
43	357349.5
44	362019.0
45	365306.0
46	371608.0
47	378436.0
48	375951.0
49	365357.5
50	356522.0
51	343348.5
52	321621.0
53	297616.0
54	274366.0
55	250780.0
56	225562.0
57	198947.0
58	173552.5
59	149563.0
60	124852.0
61	101140.0
62	78728.5
63	59369.0
64	44293.0
65	32671.0
66	24054.0
67	17921.0
68	13518.5
69	9407.5
70	6290.0
71	4581.0
72	3232.5
73	2245.0
74	1531.5
75	1038.5
76	689.0
77	443.5
78	318.5
79	250.0
80	165.0
81	109.5
82	77.0
83	49.5
84	38.5
85	29.0
86	19.5
87	14.5
88	11.0
89	9.0
90	6.5
91	3.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	4.245338989256957E-5
7	0.0
8	0.0
9	0.0
10-11	0.005672834224394609
12-13	5.306673736571197E-6
14-15	0.004086138777159822
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	199.0
20-21	738.0
22-23	1651.0
24-25	4223.0
26-27	4776.0
28-29	8318.0
30-31	18620.0
32-33	23709.0
34-35	33055.0
36-37	43301.0
38-39	50937.0
40-41	57994.0
42-43	73555.0
44-45	89816.0
46-47	87060.0
48-49	75913.0
50-51	63073.0
52-53	57778.0
54-55	65332.0
56-57	73317.0
58-59	72297.0
60-61	77173.0
62-63	79366.0
64-65	82255.0
66-67	90249.0
68-69	81006.0
70-71	79713.0
72-73	76883.0
74-75	79334.0
76-77	79940.0
78-79	86859.0
80-81	88776.0
82-83	86268.0
84-85	87780.0
86-87	92446.0
88-89	99732.0
90-91	103839.0
92-93	107243.0
94-95	123311.0
96-97	300759.0
98-99	552371.0
100-101	6061133.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.20793311673754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.04574291952368	72.3805854514167
2	7.859963928146114	12.92302777810011
3	2.200394778894942	5.426697204414416
4	0.8670539045378964	2.8511483757143012
5	0.3990324726108656	1.6401817409900217
6	0.2204695125005269	1.087460576275383
7	0.12807384931868515	0.7370080507155513
8	0.07674825734543389	0.5047452485343672
9	0.04907952851199922	0.36312539375839004
>10	0.1505880496891428	1.858758036914179
>50	0.002416350142901079	0.13005651737877982
>100	3.909741916060624E-4	0.05778449857476773
>500	2.2737298868063757E-5	0.014224508082414048
>1k	2.273728732230234E-5	0.02519661913063736
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.0613347473142393E-5	0.0	0.0	0.0
3	0.0	1.0613347473142393E-5	0.0	1.0613347473142393E-5	0.0
4	0.0	9.552012725828154E-5	0.0	4.245338989256957E-5	0.0
5	0.0	1.0613347473142394E-4	0.0	4.245338989256957E-5	0.0
6	0.0	1.0613347473142394E-4	0.0	4.245338989256957E-5	0.0
7	0.0	1.0613347473142394E-4	0.0	4.245338989256957E-5	0.0
8	0.0	1.3797351715085112E-4	0.0	4.245338989256957E-5	0.0
9	2.1226694946284787E-5	2.5472033935541747E-4	0.0	1.1674682220456632E-4	1.0613347473142393E-5
10-11	2.1226694946284787E-5	2.5472033935541747E-4	0.0	1.4858686462399353E-4	1.0613347473142393E-5
12-13	2.1226694946284787E-5	2.5472033935541747E-4	0.0	2.0165360198970547E-4	1.0613347473142393E-5
14-15	2.1226694946284787E-5	3.23707097930843E-4	0.0	2.3349364440913265E-4	1.0613347473142393E-5
16-17	2.1226694946284787E-5	3.396271191405566E-4	0.0	3.024804029845582E-4	1.0613347473142393E-5
18-19	2.6533368682855985E-5	3.6616048782341256E-4	0.0	3.396271191405566E-4	1.5920021209713588E-5
20-21	3.184004241942718E-5	4.1392055145255335E-4	0.0	4.882139837645501E-4	3.184004241942718E-5
22-23	4.245338989256957E-5	4.722939625548365E-4	0.0	7.85387713012537E-4	3.184004241942718E-5
24-25	4.245338989256957E-5	5.041340049742637E-4	0.0	0.001570775426025074	4.245338989256957E-5
26-27	4.245338989256957E-5	5.200540261839772E-4	0.0	0.0036191514883415566	4.245338989256957E-5
28-29	4.245338989256957E-5	5.200540261839772E-4	0.0	0.018350477781063197	4.776006362914077E-5
30-31	4.245338989256957E-5	5.837341110228317E-4	0.0	0.025732060948633735	5.306673736571197E-5
32-33	4.245338989256957E-5	5.996541322325453E-4	0.0	0.028788705020898742	5.306673736571197E-5
34-35	4.245338989256957E-5	7.429343231199676E-4	0.0	0.03329937769698426	5.306673736571197E-5
36-37	4.245338989256957E-5	8.596811453245339E-4	0.0	0.04006008003737596	5.306673736571197E-5
38-39	4.245338989256957E-5	8.809078402708186E-4	0.0	0.05768885019026548	5.306673736571197E-5
40-41	4.776006362914077E-5	9.870413150022425E-4	0.0	0.07717495615095492	5.306673736571197E-5
42-43	5.306673736571197E-5	0.001024188031158241	0.0	0.08853654462095384	5.306673736571197E-5
44-45	5.306673736571197E-5	0.0011621615483090922	0.0	0.10306091063794921	5.306673736571197E-5
46-47	5.306673736571197E-5	0.0012736016967770873	0.0	0.11811063735486513	5.837341110228317E-5
48-49	7.429343231199676E-5	0.0012736016967770873	0.0	0.13320281746167362	6.898675857542556E-5
50-51	9.552012725828154E-5	0.0014487219300839368	0.0	0.14827377087353583	7.429343231199675E-5
52-53	9.552012725828154E-5	0.001454028603820508	0.0	0.1876864367150501	7.429343231199675E-5
54-55	9.552012725828154E-5	0.0014752552987667927	0.0	0.23778674346201878	7.429343231199675E-5
56-57	9.552012725828154E-5	0.0015070953411862199	0.0	0.28682040878793663	8.490677978513915E-5
58-59	9.552012725828154E-5	0.0015230153623959335	0.0	0.3259783542900955	9.552012725828154E-5
60-61	9.552012725828154E-5	0.0015654687522885032	0.0	0.35333956407585654	1.0082680099485273E-4
62-63	1.1144014846799514E-4	0.001592002120971359	0.0	0.3720137489548506	1.0613347473142394E-4
64-65	1.2736016967770873E-4	0.0017936557229610646	0.0	0.3779678368872835	1.0613347473142394E-4
66-67	1.2736016967770873E-4	0.0017936557229610646	0.0	0.3833222706874838	1.0613347473142394E-4
68-69	1.2736016967770873E-4	0.0018361091128536343	0.0	0.38861833107658184	1.1144014846799513E-4
70-71	1.2736016967770873E-4	0.0018520291340633477	0.0	0.393060016994092	1.2736016967770873E-4
72-73	1.326668434142799E-4	0.0018573358077999189	0.0	0.3970028755803644	1.2736016967770873E-4
74-75	1.3797351715085112E-4	0.0018944825239559171	0.0	0.4001285064112048	1.2736016967770873E-4
76-77	1.3797351715085112E-4	0.0019581626087947714	0.0	0.40336027071677666	1.2736016967770873E-4
78-79	1.3797351715085112E-4	0.001974082630004485	0.0	0.4055307002750343	1.2736016967770873E-4
80-81	1.3797351715085112E-4	0.001974082630004485	0.0	0.40625240790320793	1.326668434142799E-4
82-83	1.3797351715085112E-4	0.0019793893037410564	0.0	0.4066557151071874	1.3797351715085112E-4
84-85	1.485868646239935E-4	0.00199530932495077	0.0	0.4068679820566502	1.3797351715085112E-4
86-87	1.485868646239935E-4	0.002048376062316482	0.0	0.406958195510172	1.4328019088742232E-4
88	1.485868646239935E-4	0.002165122884521048	0.0	0.40697942220511824	1.485868646239935E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18090	0.0	53.085613	1
GTATCAA	34270	0.0	39.057407	1
TCAACGC	43170	0.0	30.523123	4
ATCAACG	43410	0.0	30.422731	3
TATCAAC	44160	0.0	30.127352	2
CAACGCA	44095	0.0	29.875887	5
AACGCAG	44710	0.0	29.483944	6
ACGCAGA	50585	0.0	26.067905	7
CGCAGAG	51100	0.0	25.780785	8
CTTATAC	11020	0.0	24.330706	1
TGGTATC	7290	0.0	24.014671	2
GTGGTAT	7795	0.0	23.385578	1
GCAGAGT	57285	0.0	22.902403	9
GTACATG	35855	0.0	21.971867	1
TACATGG	35580	0.0	21.568441	2
TATACAC	14695	0.0	21.51346	3
GAGTACT	37390	0.0	21.105724	12-13
ACATGGG	35820	0.0	20.771484	3
CAGAGTA	56235	0.0	19.951767	10-11
AGAGTAC	53710	0.0	19.59204	10-11
>>END_MODULE