FastQCFastQC Report
Fri 27 May 2016
SRR2139212_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139212_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8940825
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119500.052.0230751
GTATCAA221700.038.1330381
TCAACGC271050.030.4530564
ATCAACG276100.029.9116233
TATCAAC281850.029.470952
CAACGCA280650.029.4288235
AACGCAG285700.028.9542646
ACGCAGA324000.025.4913587
CGCAGAG350850.023.5408068
GTGGTAT56800.023.0984521
GTACATG293450.022.9171751
CTTATAC105100.022.7757721
TGGTATC55700.022.4626792
TACATGG294900.022.1857682
ACATGGG295000.021.471373
GCAGAGT386850.021.3069789
TATACAC138550.019.879573
GAGTACT225300.019.81464212-13
AGAGTAC350000.019.2548310-11
CAGAGTA375750.019.1494910-11
Produced by FastQC (version 0.11.5)