##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139212_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8940825
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60921905976238	33.0	33.0	33.0	33.0	33.0
2	32.61345558155987	33.0	33.0	33.0	33.0	33.0
3	32.61545606809215	33.0	33.0	33.0	33.0	33.0
4	36.519362586785896	37.0	37.0	37.0	37.0	37.0
5	36.54106181476541	37.0	37.0	37.0	37.0	37.0
6	36.52996753655283	37.0	37.0	37.0	37.0	37.0
7	36.49555382193478	37.0	37.0	37.0	37.0	37.0
8	36.49309856752593	37.0	37.0	37.0	37.0	37.0
9	36.53556019718538	37.0	37.0	37.0	37.0	37.0
10-11	36.45593678435715	37.0	37.0	37.0	37.0	37.0
12-13	36.45400737627679	37.0	37.0	37.0	37.0	37.0
14-15	38.50961801623452	40.0	37.0	40.0	37.0	40.0
16-17	38.44699845931444	40.0	37.0	40.0	37.0	40.0
18-19	38.46152060911605	40.0	37.0	40.0	37.0	40.0
20-21	38.446930467413495	40.0	37.0	40.0	37.0	40.0
22-23	38.45194277749125	40.0	37.0	40.0	37.0	40.0
24-25	38.4388594952601	40.0	37.0	40.0	37.0	40.0
26-27	38.33119417847004	40.0	37.0	40.0	37.0	40.0
28-29	38.25622628412977	40.0	37.0	40.0	37.0	40.0
30-31	38.2012329754906	40.0	37.0	40.0	37.0	40.0
32-33	38.13085488756418	40.0	37.0	40.0	37.0	40.0
34-35	38.091379652343434	40.0	37.0	40.0	33.0	40.0
36-37	38.024421245061106	40.0	37.0	40.0	33.0	40.0
38-39	37.9601823478215	40.0	37.0	40.0	33.0	40.0
40-41	37.926241727464074	40.0	37.0	40.0	33.0	40.0
42-43	37.8656382491084	40.0	37.0	40.0	33.0	40.0
44-45	37.79082386830164	40.0	37.0	40.0	33.0	40.0
46-47	37.74151347547726	40.0	37.0	40.0	33.0	40.0
48-49	37.70344744822091	40.0	37.0	40.0	33.0	40.0
50-51	37.60502731356645	40.0	37.0	40.0	33.0	40.0
52-53	37.49282584528562	40.0	37.0	40.0	33.0	40.0
54-55	37.29000147003786	40.0	37.0	40.0	33.0	40.0
56-57	37.1992233431566	38.5	37.0	40.0	33.0	40.0
58-59	37.06429054068035	37.0	37.0	40.0	33.0	40.0
60-61	36.91520108205666	37.0	37.0	40.0	33.0	40.0
62-63	36.746617394402065	37.0	37.0	40.0	33.0	40.0
64-65	36.55775654534857	37.0	37.0	40.0	33.0	40.0
66-67	36.34740383704805	37.0	37.0	40.0	33.0	40.0
68-69	36.14354724043483	37.0	37.0	40.0	33.0	40.0
70-71	35.8781926386993	37.0	35.0	37.0	33.0	40.0
72-73	35.670366021778975	37.0	33.0	37.0	33.0	40.0
74-75	35.494115774198264	37.0	33.0	37.0	33.0	40.0
76-77	33.84299020710563	35.0	33.0	37.0	27.0	37.0
78-79	34.98664174879896	37.0	33.0	37.0	33.0	37.0
80-81	35.05856430551551	37.0	33.0	37.0	33.0	37.0
82-83	34.94354762953052	37.0	33.0	37.0	33.0	37.0
84-85	34.785977768494085	37.0	33.0	37.0	33.0	37.0
86-87	34.71503322407753	37.0	33.0	37.0	33.0	37.0
88-89	34.61931469157255	37.0	33.0	37.0	33.0	37.0
90-91	34.51122779921076	37.0	33.0	37.0	33.0	37.0
92-93	34.48065159935708	37.0	33.0	37.0	33.0	37.0
94-95	34.50832661044664	37.0	33.0	37.0	33.0	37.0
96-97	34.5175038370996	37.0	33.0	37.0	33.0	37.0
98-99	34.50539920309837	37.0	33.0	37.0	33.0	37.0
100	34.48660792938689	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	8.0
19	7.0
20	56.0
21	375.0
22	1465.0
23	4214.0
24	9658.0
25	18884.0
26	32644.0
27	51402.0
28	74453.0
29	102957.0
30	137343.0
31	180885.0
32	237956.0
33	316626.0
34	434120.0
35	630969.0
36	1134126.0
37	2652697.0
38	2859335.0
39	60644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.12050901342997	19.72414178781041	14.046455444547904	24.108893754211717
2	14.87184907433039	20.89497333859012	41.34820891808083	22.884968668998667
3	18.309742109928333	25.994402082581864	30.130116627939817	25.565739179549986
4	12.57366070804428	18.037250477444754	38.03354835823316	31.355540456277804
5	13.774892138029768	37.31943081315203	35.11852653418448	13.787150514633717
6	30.292731932058043	37.25172266309711	18.59905726641261	13.856488138432233
7	26.75518198823934	32.3437714081195	22.735284495558293	18.165762108082866
8	24.807341604382145	36.2679730338084	20.764839933675024	18.159845428134428
9	25.69554822960968	16.618231539035826	20.878979288824016	36.80724094253047
10-11	24.56358365161432	26.542137162611574	28.250046740368894	20.64423244540521
12-13	25.401769456595947	24.092097104249007	28.35368417944476	22.15244925971029
14-15	22.5071569388094	25.274366797776775	26.117310992025622	26.1011652713882
16-17	22.148403530994063	28.565853822214393	26.61872366364402	22.66701898314753
18-19	22.16922375731546	27.43759105004292	28.418512832988007	21.97467235965361
20-21	23.04678167522542	26.588207305320744	28.434351704458514	21.930659314995317
22-23	22.855229407745302	26.644019814252708	28.199218398907394	22.301532379094596
24-25	22.60894519630288	26.777712669028297	28.434027044527394	22.179315090141433
26-27	22.529567940260108	26.887718769295027	28.37325882410479	22.209454466340073
28-29	22.423036943151853	26.75500727836183	28.386534899046566	22.43542087943975
30-31	22.837369214490895	26.566311684866317	28.35086704285588	22.245452057786906
32-33	22.75867260823224	26.61762131759894	28.33726060652877	22.28644546764005
34-35	22.582756890656867	26.773271919633306	28.399678325265864	22.244292864443967
36-37	22.85358494651124	26.705671879283383	28.02635772699034	22.41438544721504
38-39	22.422833921590577	26.91355806601989	28.254812917193878	22.408795095195654
40-41	22.632034599494474	26.711429070757298	28.229630005468454	22.426906324279773
42-43	22.566681429040536	26.78311767596574	28.026340074715627	22.623860820278097
44-45	22.47649889036185	26.962550195069134	27.840117823522093	22.72083309104692
46-47	22.792078279970156	26.85948870321202	27.469123812340975	22.879309204476847
48-49	22.82965588704525	27.0946049173566	27.28212508191578	22.793614113682377
50-51	22.69357754571874	27.185449825041335	27.35392439494701	22.76704823429291
52-53	22.924418690333376	27.153348894939157	27.331783170276598	22.590449244450873
54-55	22.959806029006387	27.0850042983399	27.13187959030626	22.823310082347454
56-57	23.022733593264	27.308331874147697	26.92086119659291	22.7480733359954
58-59	22.65951935352679	27.32468547376939	27.26312433754046	22.75267083516336
60-61	22.978585076014802	27.41551853636497	27.010081633001576	22.595814754618658
62-63	22.749845795566486	27.527203748017055	27.087721874913655	22.6352285815028
64-65	22.860829705340233	27.345709984947312	27.007439336723348	22.786020972989103
66-67	22.89600278206522	27.36007395714522	26.95058160909444	22.793341651695123
68-69	22.86871760629649	27.381478280214772	26.955644825177572	22.794159288311164
70-71	22.883240890067526	27.20568130672219	26.83116874619451	23.079909057015772
72-73	22.87419081916098	27.255576635051227	26.699955551580924	23.170276994206876
74-75	22.776881406542344	27.33717476625842	26.8597546708574	23.026189156341832
76-77	22.89904029525023	27.195714549422444	26.78634943646852	23.118895718858802
78-79	22.759804874052563	27.21952654231123	26.680101786131168	23.340566797505044
80-81	22.95325537181798	27.324805827000088	26.739042387865574	22.982896413316357
82-83	23.116394474170647	27.1007965482973	26.859009719116216	22.923799258415837
84-85	22.95345265394703	27.111885220111056	26.77125776786419	23.163404358077734
86-87	22.818229137514216	27.37798704047497	26.785447230306236	23.01833659170458
88-89	23.116501497690106	27.236954450230357	26.68083680080698	22.965707251272562
90-91	23.1021852816988	27.262999270225762	26.69349092390731	22.94132452416812
92-93	22.98076907788893	27.166706173595312	26.83663840072082	23.015886347794943
94-95	22.90226930267381	27.4414483584635	26.87151826480424	22.78476407405845
96-97	23.09071497292989	27.322139887144438	26.94731970703504	22.639825432890635
98-99	24.106836096252216	29.34769723056047	27.119917839816114	19.4255488333712
100	22.891444410965597	30.443878203481418	21.059825441392682	25.604851944160306
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2926.0
1	2427.0
2	2880.0
3	3951.0
4	5081.0
5	8207.5
6	11018.5
7	13194.0
8	14741.5
9	14478.0
10	12910.0
11	11319.5
12	12008.5
13	15867.5
14	24937.5
15	37266.5
16	47494.0
17	53431.0
18	54383.0
19	50862.0
20	44760.5
21	38537.5
22	34627.0
23	34894.5
24	39574.5
25	48753.0
26	61253.5
27	74715.5
28	89626.0
29	106752.0
30	123456.0
31	139541.5
32	156922.0
33	174573.5
34	191862.0
35	208599.0
36	227090.0
37	244319.5
38	257767.0
39	271542.5
40	285333.5
41	297866.5
42	306705.0
43	313619.0
44	321763.0
45	334838.5
46	348023.5
47	362299.5
48	371704.0
49	374751.5
50	381310.0
51	379810.5
52	357905.5
53	333747.5
54	318977.0
55	301818.0
56	281310.5
57	256198.0
58	230022.5
59	201738.0
60	170522.0
61	144571.5
62	119031.5
63	96019.5
64	78504.0
65	61443.5
66	49055.0
67	36339.0
68	23740.0
69	17362.5
70	12410.0
71	7569.0
72	5077.0
73	3632.5
74	2796.5
75	2080.0
76	1298.0
77	672.0
78	477.0
79	366.5
80	234.0
81	143.0
82	112.0
83	73.5
84	44.5
85	33.5
86	24.0
87	17.5
88	14.0
89	10.5
90	6.0
91	3.5
92	3.5
93	3.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.901390531634385E-4
7	0.0
8	0.0
9	0.0
10-11	0.005374224414413659
12-13	1.1184650186084617E-5
14-15	0.004199836144874774
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	158.0
20-21	655.0
22-23	1322.0
24-25	2592.0
26-27	3758.0
28-29	6632.0
30-31	14918.0
32-33	18448.0
34-35	25485.0
36-37	35236.0
38-39	40454.0
40-41	44501.0
42-43	54831.0
44-45	65280.0
46-47	63776.0
48-49	56374.0
50-51	47323.0
52-53	44690.0
54-55	50951.0
56-57	55098.0
58-59	36861.0
60-61	59542.0
62-63	59531.0
64-65	62280.0
66-67	67179.0
68-69	60482.0
70-71	59459.0
72-73	56708.0
74-75	58541.0
76-77	57596.0
78-79	62590.0
80-81	64088.0
82-83	63229.0
84-85	64305.0
86-87	69055.0
88-89	74658.0
90-91	79015.0
92-93	82933.0
94-95	104542.0
96-97	327557.0
98-99	568459.0
100-101	6169733.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.86965687101821
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.59143960072154	75.14003297893068
2	7.102106480074928	11.913024670906381
3	1.7616928355246335	4.432577208827464
4	0.6694814474954351	2.2459671713181892
5	0.3014225385242804	1.2640102439611332
6	0.1684958875646532	0.8479015364535077
7	0.11041901099124363	0.6482563194710954
8	0.06908549169708286	0.4635341186719934
9	0.04937312672771974	0.37268164775728374
>10	0.17126869970979244	2.2738560610360126
>50	0.00417191725985984	0.238746623123577
>100	9.696269408413717E-4	0.11346547797832988
>500	7.33367680466802E-5	0.045945941564229806
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.355395055825385E-5	0.0	0.0	0.0
3	0.0	3.355395055825385E-5	0.0	0.0	0.0
4	0.0	1.1184650186084617E-4	0.0	0.0	0.0
5	0.0	1.1184650186084617E-4	0.0	0.0	0.0
6	2.2369300372169234E-5	1.1184650186084617E-4	0.0	0.0	1.1184650186084617E-5
7	2.2369300372169234E-5	1.1184650186084617E-4	0.0	0.0	2.2369300372169234E-5
8	2.2369300372169234E-5	1.342158022330154E-4	0.0	0.0	2.2369300372169234E-5
9	2.2369300372169234E-5	3.019855550242847E-4	0.0	3.355395055825385E-5	2.2369300372169234E-5
10-11	2.2369300372169234E-5	3.131702052103693E-4	0.0	6.71079011165077E-5	2.2369300372169234E-5
12-13	2.2369300372169234E-5	3.131702052103693E-4	0.0	7.270022620955001E-5	2.2369300372169234E-5
14-15	2.2369300372169234E-5	4.5297833253642704E-4	0.0	8.388487639563464E-5	2.7961625465211544E-5
16-17	1.0066185167476156E-4	4.809399580016386E-4	0.0	8.947720148867694E-5	4.473860074433847E-5
18-19	1.0066185167476156E-4	5.312708838390194E-4	0.0	8.947720148867694E-5	4.473860074433847E-5
20-21	1.0066185167476156E-4	5.816018096764001E-4	0.0	8.947720148867694E-5	4.473860074433847E-5
22-23	1.0625417676780386E-4	6.375250606068233E-4	0.0	1.1184650186084617E-4	5.0330925837380774E-5
24-25	1.1184650186084617E-4	7.158176119094155E-4	0.0	1.1184650186084617E-4	6.71079011165077E-5
26-27	1.1184650186084617E-4	7.437792373746271E-4	0.0	2.2369300372169235E-4	6.71079011165077E-5
28-29	1.1184650186084617E-4	7.493715624676694E-4	0.0	5.871941347694424E-4	6.71079011165077E-5
30-31	1.230311520469308E-4	8.724027145146001E-4	0.0	0.0017671747294013694	6.71079011165077E-5
32-33	1.230311520469308E-4	8.724027145146001E-4	0.0	0.004574521926108609	6.71079011165077E-5
34-35	1.230311520469308E-4	0.0010345801422128272	0.0	0.007818070480073148	6.71079011165077E-5
36-37	1.230311520469308E-4	0.0011911652448180119	0.0	0.013063671417346833	6.71079011165077E-5
38-39	1.230311520469308E-4	0.0012359038455623502	0.0	0.02928700651226257	6.71079011165077E-5
40-41	1.230311520469308E-4	0.0013197887219579848	0.0	0.04687486892988063	6.71079011165077E-5
42-43	1.230311520469308E-4	0.0013421580223301542	0.0	0.05638182158805256	6.71079011165077E-5
44-45	1.286234771399731E-4	0.0014651891743770848	0.0	0.06921620767658465	7.270022620955001E-5
46-47	1.342158022330154E-4	0.0015658510260518465	0.0	0.08210092469095413	9.506952658171925E-5
48-49	1.342158022330154E-4	0.0015658510260518465	0.0	0.09546098933823222	1.0066185167476156E-4
50-51	1.5099277751214234E-4	0.0017224361286570311	0.0	0.10729434923510973	1.0066185167476156E-4
52-53	1.5658510260518465E-4	0.0017336207788431156	0.0	0.14135720137683044	1.1743882695388849E-4
54-55	1.5658510260518465E-4	0.0017559900792152847	0.0	0.18378057953264937	1.342158022330154E-4
56-57	1.5658510260518465E-4	0.001811913330145708	0.0	0.22710432202844816	1.342158022330154E-4
58-59	1.5658510260518465E-4	0.001828690305424835	0.0	0.37445649590501995	1.342158022330154E-4
60-61	1.5658510260518465E-4	0.0018454672807039618	0.0	0.49915975315477035	1.342158022330154E-4
62-63	1.901390531634385E-4	0.0019013905316343852	0.0	0.5052050565803492	1.342158022330154E-4
64-65	1.901390531634385E-4	0.002102714234983908	0.0	0.5101318949873195	1.342158022330154E-4
66-67	2.0132370334952312E-4	0.00213067586044912	0.0	0.5142254769554264	1.398081273260577E-4
68-69	2.1250835353560772E-4	0.0021865991113795427	0.0	0.5183246512486264	1.4540045241910002E-4
70-71	2.1250835353560772E-4	0.0021977837615656274	0.0	0.5216241230535213	1.5658510260518465E-4
72-73	2.1250835353560772E-4	0.0022145607368447544	0.0	0.5243699546742051	1.901390531634385E-4
74-75	2.1250835353560772E-4	0.0022648916626821346	0.0	0.5268529470155159	1.901390531634385E-4
76-77	2.1250835353560772E-4	0.002387922814729066	0.0	0.5292632391306171	1.957313782564808E-4
78-79	2.1250835353560772E-4	0.0024382537405664467	0.0	0.5307899438810177	2.0132370334952312E-4
80-81	2.1250835353560772E-4	0.0024382537405664467	0.0	0.5312149605880889	2.2369300372169235E-4
82-83	2.1250835353560772E-4	0.0024606230409386157	0.0	0.5315225384682063	2.404699790008193E-4
84-85	2.1250835353560772E-4	0.002482992341310785	0.0	0.5317462314719279	2.460623040938616E-4
86-87	2.1810067862865004E-4	0.002516546291869039	0.0	0.5318748549490679	2.572469542799462E-4
88	2.2369300372169235E-4	0.0025948388431716314	0.0	0.531886039599254	2.572469542799462E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11950	0.0	52.023075	1
GTATCAA	22170	0.0	38.133038	1
TCAACGC	27105	0.0	30.453056	4
ATCAACG	27610	0.0	29.911623	3
TATCAAC	28185	0.0	29.47095	2
CAACGCA	28065	0.0	29.428823	5
AACGCAG	28570	0.0	28.954264	6
ACGCAGA	32400	0.0	25.491358	7
CGCAGAG	35085	0.0	23.540806	8
GTGGTAT	5680	0.0	23.098452	1
GTACATG	29345	0.0	22.917175	1
CTTATAC	10510	0.0	22.775772	1
TGGTATC	5570	0.0	22.462679	2
TACATGG	29490	0.0	22.185768	2
ACATGGG	29500	0.0	21.47137	3
GCAGAGT	38685	0.0	21.306978	9
TATACAC	13855	0.0	19.87957	3
GAGTACT	22530	0.0	19.814642	12-13
AGAGTAC	35000	0.0	19.25483	10-11
CAGAGTA	37575	0.0	19.14949	10-11
>>END_MODULE