FastQCFastQC Report
Fri 27 May 2016
SRR2139211_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139211_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9727710
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128650.048.506711
GTATCAA229800.038.52371
TCAACGC272700.031.4711994
ATCAACG275450.031.1409023
CAACGCA277400.030.9246945
AACGCAG285700.030.1194426
TATCAAC291550.029.7559132
ACGCAGA327000.026.3018027
CGCAGAG339500.025.333928
GTACATG307150.022.9451481
TACATGG309400.022.2192232
ACATGGG311650.021.404163
GCAGAGT400300.021.387759
GAGTACT216650.020.81153512-13
CATGGGG227500.019.3396134
CAGAGTA381850.019.1741510-11
AGAGTAC350200.019.0323210-11
GTGGTAT72000.018.7881851
GTACTTT248300.017.9981214-15
TGGTATC70000.017.4241732
Produced by FastQC (version 0.11.5)