FastQCFastQC Report
Fri 27 May 2016
SRR2139210_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139210_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11889882
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA197750.053.6800651
GTATCAA359150.037.539311
TCAACGC442950.030.002534
ATCAACG443150.029.940133
CAACGCA452000.029.3847035
TATCAAC456150.029.227782
AACGCAG469200.028.3531886
GTGGTAT83150.027.7283171
TGGTATC83850.026.0657712
ACGCAGA536400.024.8010047
CGCAGAG543750.024.4502058
GTACATG459500.021.544181
GCAGAGT620750.021.2178949
TACATGG462200.021.1865252
GAGTACT356650.020.94483412-13
ACATGGG469950.020.299113
CTTATAC116300.020.0826151
CAGAGTA606000.019.50274310-11
GTACTTT392550.019.02975814-15
AGAGTAC571650.018.474110-11
Produced by FastQC (version 0.11.5)