##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139210_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11889882
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.518240719293935	33.0	33.0	33.0	33.0	33.0
2	32.54805859301211	33.0	33.0	33.0	33.0	33.0
3	32.562138127190835	33.0	33.0	33.0	33.0	33.0
4	36.46767276580205	37.0	37.0	37.0	37.0	37.0
5	36.49653150468608	37.0	37.0	37.0	37.0	37.0
6	36.48823646862097	37.0	37.0	37.0	37.0	37.0
7	36.46464716807114	37.0	37.0	37.0	37.0	37.0
8	36.45770016893355	37.0	37.0	37.0	37.0	37.0
9	36.49683386260688	37.0	37.0	37.0	37.0	37.0
10-11	36.41732352768514	37.0	37.0	37.0	37.0	37.0
12-13	36.418112139380355	37.0	37.0	37.0	37.0	37.0
14-15	38.38752125546746	40.0	37.0	40.0	37.0	40.0
16-17	38.33890832558305	40.0	37.0	40.0	37.0	40.0
18-19	38.35209865833824	40.0	37.0	40.0	37.0	40.0
20-21	38.353095142265595	40.0	37.0	40.0	37.0	40.0
22-23	38.35330937739287	40.0	37.0	40.0	37.0	40.0
24-25	38.33833937313814	40.0	37.0	40.0	37.0	40.0
26-27	38.22304420479689	40.0	37.0	40.0	37.0	40.0
28-29	38.15344029118138	40.0	37.0	40.0	35.0	40.0
30-31	37.99987558392155	40.0	37.0	40.0	33.0	40.0
32-33	38.00133056014445	40.0	37.0	40.0	33.0	40.0
34-35	37.96087586942405	40.0	37.0	40.0	33.0	40.0
36-37	37.874952042948756	40.0	37.0	40.0	33.0	40.0
38-39	37.81786518321397	40.0	37.0	40.0	33.0	40.0
40-41	37.821900225092485	40.0	37.0	40.0	33.0	40.0
42-43	37.75442218694346	40.0	37.0	40.0	33.0	40.0
44-45	37.68637140752628	40.0	37.0	40.0	33.0	40.0
46-47	37.64214537852608	40.0	37.0	40.0	33.0	40.0
48-49	37.616776742046646	40.0	37.0	40.0	33.0	40.0
50-51	37.52457152787827	40.0	37.0	40.0	33.0	40.0
52-53	37.42061193525285	40.0	37.0	40.0	33.0	40.0
54-55	37.27138243503295	40.0	37.0	40.0	33.0	40.0
56-57	37.164717213023806	38.5	37.0	40.0	33.0	40.0
58-59	37.001081590657336	37.0	37.0	40.0	33.0	40.0
60-61	36.88363432399497	37.0	37.0	40.0	33.0	40.0
62-63	36.71950305249236	37.0	37.0	40.0	33.0	40.0
64-65	36.541626842648256	37.0	37.0	40.0	33.0	40.0
66-67	36.316269371624244	37.0	37.0	40.0	33.0	40.0
68-69	36.10037032897691	37.0	37.0	40.0	33.0	40.0
70-71	35.84784830860244	37.0	35.0	37.0	33.0	40.0
72-73	35.63560484776216	37.0	33.0	37.0	33.0	40.0
74-75	35.415904256586174	37.0	33.0	37.0	33.0	40.0
76-77	33.72929601233997	35.0	33.0	37.0	27.0	37.0
78-79	34.93141684814112	37.0	33.0	37.0	33.0	37.0
80-81	35.006692339469154	37.0	33.0	37.0	33.0	37.0
82-83	34.85790344569098	37.0	33.0	37.0	33.0	37.0
84-85	34.73263226035645	37.0	33.0	37.0	33.0	37.0
86-87	34.62160253884197	37.0	33.0	37.0	33.0	37.0
88-89	34.471783502175256	37.0	33.0	37.0	30.0	37.0
90-91	34.46612180173941	37.0	33.0	37.0	33.0	37.0
92-93	34.39879496386628	37.0	33.0	37.0	33.0	37.0
94-95	34.37546476854248	37.0	33.0	37.0	33.0	37.0
96-97	34.38692848638978	37.0	33.0	37.0	33.0	37.0
98-99	34.38940092078341	37.0	33.0	37.0	33.0	37.0
100	34.40830400460226	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	9.0
19	17.0
20	118.0
21	533.0
22	2036.0
23	5848.0
24	13386.0
25	26088.0
26	44917.0
27	70584.0
28	103070.0
29	143971.0
30	194299.0
31	256292.0
32	336756.0
33	446830.0
34	609789.0
35	874344.0
36	1489873.0
37	3426571.0
38	3750910.0
39	93641.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.080249408698926	20.032839686718507	14.378124189962524	23.508786714620044
2	14.837127904213009	20.818650681310377	41.995706938050354	22.34851447642626
3	18.635113451924923	26.139014668101833	30.163924250888275	25.061947629084962
4	12.586172007426146	18.14756445858756	38.26924438779123	30.997019146195058
5	13.567914298897163	37.57708444877754	35.05700897620346	13.797992276121832
6	29.8769666434396	37.49886142525588	18.691061673114035	13.933110258190485
7	26.621466890924566	32.65179587148131	22.542309503155707	18.184427734438408
8	24.427197847716233	36.79830464255238	20.815513560185035	17.958983949546344
9	25.697673029892137	16.92821678129354	20.828070455198798	36.54603973361552
10-11	24.536779610993868	26.767808070145012	28.197742414331657	20.497669904529463
12-13	25.533914180287287	24.327716034788914	28.42442719560056	21.71394258932324
14-15	22.56756285643768	25.585856933028143	26.328980332287504	25.51759987824667
16-17	21.828854146744263	28.789234409559324	27.003367232744612	22.378544210951798
18-19	21.84596112896663	27.522035121963363	29.084615810316706	21.547387938753303
20-21	22.78698605748744	26.6201687756367	29.11188178648827	21.480963380387593
22-23	22.495941938727942	26.789996569846853	28.91618684055979	21.79787465086542
24-25	22.221304281693403	26.934011424040964	29.074530021927377	21.77015427233826
26-27	22.146336865593796	27.11644972162068	29.01998233974964	21.717231073035887
28-29	22.035005667293557	27.031489524715607	29.072809725223088	21.860695082767744
30-31	22.222633293173853	27.013340778680373	29.053796789893564	21.710229138252206
32-33	22.047549453361448	27.19003462011793	28.94801005027756	21.81440587624306
34-35	22.278444153567914	27.0871773208376	28.942048323640645	21.692330201953837
36-37	22.110002380985538	27.15604863731884	28.77076696605041	21.963182015645213
38-39	21.97469002370611	27.126203300939604	28.914038430981954	21.985068244372332
40-41	22.18934935352765	26.985302598023175	28.878425557578137	21.946922490871035
42-43	22.162799853906165	27.066895241458987	28.645901507327416	22.124403397307432
44-45	22.194371168987946	27.265370113021	28.363047664319087	22.177211053671968
46-47	22.420403729873804	27.211413337276163	27.903475032633278	22.464707900216755
48-49	22.291559348430987	27.389594778151505	27.820464946882346	22.498380926535162
50-51	22.29279212196428	27.570688325332338	27.684963007554686	22.451556545148694
52-53	22.381877013932122	27.560589768259124	27.707659028210095	22.349874189598655
54-55	22.415594839317208	27.498401616031288	27.609253341327666	22.476750203323842
56-57	22.482772438634	27.62590475149644	27.435548907240058	22.4557739026295
58-59	22.331853477370853	27.719157311858535	27.60077464805539	22.348214562715228
60-61	22.492028355837576	27.812657271163776	27.414794479581477	22.280519893417168
62-63	22.334779678876217	27.933886872371357	27.374917353460038	22.356416095292396
64-65	22.383139329805996	27.848413874191653	27.318048206937096	22.45039858906526
66-67	22.452385597976765	27.786325365195193	27.209238627572972	22.552050409255067
68-69	22.431725132539416	27.72845770357647	27.297379396918714	22.542437766965403
70-71	22.472938878292677	27.515668085057822	27.37425612803684	22.63713690861266
72-73	22.46911906743697	27.508054420980017	27.28706958631315	22.735756925269865
74-75	22.423399501058032	27.647960551696176	27.225155992587986	22.703483954657802
76-77	22.58734908894448	27.55471625500425	27.195697215421905	22.66223744062936
78-79	22.52238544526689	27.525928393246268	27.19229368958201	22.759392471904828
80-81	22.486584881104413	27.616485630064048	27.153814120404284	22.743115368427258
82-83	22.602266326754496	27.53045558218965	27.253540137798694	22.613737953257164
84-85	22.653747074941812	27.527135789325207	27.190087006024843	22.629030129708138
86-87	22.518404475995307	27.52422972195942	27.34573231782943	22.61163348421584
88-89	22.620682337967402	27.45604427771939	27.27035963282819	22.652913751485023
90-91	22.658479938282753	27.430628965720803	27.30782161866291	22.603069477333538
92-93	22.65666904175068	27.389115231129228	27.381414014890247	22.572801712229847
94-95	22.535152982610608	27.46185340083645	27.391954655513977	22.61103896103896
96-97	22.61552520168769	27.521944875267945	27.471641500575927	22.390888422468436
98-99	23.751787190751124	29.43768085748319	27.638295462647218	19.17223648911847
100	22.703748921346158	30.393058263287415	21.490376809741445	25.41281600562498
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2448.0
1	2193.0
2	3109.5
3	4610.5
4	6394.0
5	10808.5
6	14610.0
7	17154.5
8	18840.0
9	18747.5
10	17606.0
11	16285.0
12	17898.0
13	24232.5
14	38947.0
15	59444.5
16	76755.0
17	87334.0
18	89723.5
19	84054.0
20	73347.0
21	60537.0
22	50071.0
23	46505.0
24	50148.5
25	59781.0
26	74724.0
27	91924.0
28	111795.0
29	136108.5
30	161938.0
31	189675.5
32	221403.0
33	252748.5
34	282895.0
35	312103.5
36	342687.0
37	371767.0
38	394614.5
39	412558.0
40	428412.0
41	442549.5
42	453427.0
43	465924.0
44	476545.0
45	487172.5
46	498904.5
47	508188.0
48	509493.5
49	502319.0
50	493980.0
51	480193.5
52	458205.0
53	432156.0
54	406012.5
55	375990.5
56	343793.0
57	311229.0
58	276014.5
59	239781.0
60	201326.5
61	163700.0
62	129777.5
63	99918.0
64	74590.5
65	55106.0
66	41352.5
67	31415.5
68	23632.0
69	16982.0
70	12123.0
71	8726.0
72	6079.5
73	4168.5
74	2842.0
75	1926.0
76	1272.0
77	817.0
78	523.5
79	344.5
80	229.5
81	155.5
82	121.0
83	91.5
84	68.0
85	50.5
86	36.5
87	27.5
88	21.0
89	12.0
90	5.5
91	4.0
92	3.5
93	2.5
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.4297871080638143E-4
7	0.0
8	0.0
9	0.0
10-11	0.0038267831421707967
12-13	2.5231537201126132E-5
14-15	0.0022582225795007888
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	180.0
20-21	735.0
22-23	1373.0
24-25	3865.0
26-27	4552.0
28-29	8709.0
30-31	19852.0
32-33	25361.0
34-35	36799.0
36-37	51922.0
38-39	62231.0
40-41	68807.0
42-43	85886.0
44-45	104346.0
46-47	101633.0
48-49	90053.0
50-51	74774.0
52-53	69218.0
54-55	77653.0
56-57	85702.0
58-59	86683.0
60-61	87798.0
62-63	88285.0
64-65	91915.0
66-67	102049.0
68-69	95800.0
70-71	94572.0
72-73	89341.0
74-75	91684.0
76-77	90320.0
78-79	98964.0
80-81	102172.0
82-83	98576.0
84-85	99869.0
86-87	106998.0
88-89	116337.0
90-91	123847.0
92-93	130583.0
94-95	148774.0
96-97	417362.0
98-99	732052.0
100-101	7822250.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.91156907024741
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.01465941920567	72.97433513629771
2	8.268104129041301	13.710429731500112
3	2.072512144574037	5.1550570147133055
4	0.7712846837075458	2.5579369330416837
5	0.35295733728952877	1.4632123324765687
6	0.17868275135737796	0.8888920368497452
7	0.10529382818792749	0.6111053555931879
8	0.0657031784668671	0.43580428956723544
9	0.04020586310506375	0.30001780762779645
>10	0.1270820716803578	1.6201163448664124
>50	0.0028665231510611863	0.16070169521058003
>100	5.776759304781514E-4	0.08598705261932198
>500	7.03943027110717E-5	0.03640426963641886
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.6821024800750755E-5	0.0	8.410512400375377E-6	0.0
3	0.0	1.6821024800750755E-5	0.0	8.410512400375377E-6	0.0
4	0.0	3.364204960150151E-5	0.0	8.410512400375377E-6	0.0
5	0.0	3.364204960150151E-5	0.0	8.410512400375377E-6	0.0
6	1.0092614880450454E-4	3.364204960150151E-5	0.0	8.410512400375377E-6	8.410512400375377E-6
7	1.0092614880450454E-4	3.364204960150151E-5	0.0	8.410512400375377E-6	8.410512400375377E-6
8	1.0092614880450454E-4	4.205256200187689E-5	0.0	8.410512400375377E-6	8.410512400375377E-6
9	1.0092614880450454E-4	1.0933666120487991E-4	0.0	8.410512400375377E-6	8.410512400375377E-6
10-11	1.0933666120487991E-4	1.0933666120487991E-4	0.0	2.9436793401313822E-5	8.410512400375377E-6
12-13	1.1774717360525529E-4	1.0933666120487991E-4	0.0	6.307884300281534E-5	8.410512400375377E-6
14-15	1.2615768600563067E-4	1.8082601660807064E-4	0.0	8.831038020394146E-5	8.410512400375377E-6
16-17	1.6821024800750756E-4	1.8503127280825832E-4	0.0	1.0513140500469223E-4	8.410512400375377E-6
18-19	1.6821024800750756E-4	2.0605755380919677E-4	0.0	1.6400499180731985E-4	8.410512400375377E-6
20-21	1.6821024800750756E-4	2.3549434721051058E-4	0.0	2.3549434721051058E-4	8.410512400375377E-6
22-23	1.6821024800750756E-4	2.649311406118244E-4	0.0	3.8688357041726743E-4	1.2615768600563066E-5
24-25	1.6821024800750756E-4	2.9436793401313823E-4	0.0	9.209511078411039E-4	1.6821024800750755E-5
26-27	1.6821024800750756E-4	3.1118895881388896E-4	0.0	0.002666132430918995	1.6821024800750755E-5
28-29	1.6821024800750756E-4	3.1118895881388896E-4	0.0	0.007148935540319071	1.6821024800750755E-5
30-31	1.6821024800750756E-4	3.5324152081576585E-4	0.0	0.008864680069995648	1.6821024800750755E-5
32-33	1.7241550420769524E-4	3.616520332161413E-4	0.0	0.012645205393964382	1.6821024800750755E-5
34-35	1.89236529008446E-4	4.205256200187689E-4	0.0	0.01825081190881457	1.6821024800750755E-5
36-37	1.934417852086337E-4	4.7098869442102116E-4	0.0	0.027456117731025423	1.6821024800750755E-5
38-39	1.934417852086337E-4	4.8360446302158424E-4	0.0	0.048810408715578506	1.6821024800750755E-5
40-41	1.9764704140882137E-4	5.130412564228981E-4	0.0	0.07087538799796331	1.6821024800750755E-5
42-43	2.0185229760900908E-4	5.214517688232735E-4	0.0	0.08526997997120578	1.6821024800750755E-5
44-45	2.0185229760900908E-4	5.845306118260888E-4	0.0	0.10276384576398656	1.6821024800750755E-5
46-47	2.0185229760900908E-4	6.391989424285288E-4	0.0	0.12101045241660094	1.6821024800750755E-5
48-49	2.0185229760900908E-4	6.434041986287164E-4	0.0	0.13864309166398792	2.5231537201126135E-5
50-51	2.354943472105106E-4	7.106882978317195E-4	0.0	0.1546020389437002	2.5231537201126135E-5
52-53	2.4390485961088597E-4	7.148935540319071E-4	0.0	0.1893080183638492	2.5231537201126135E-5
54-55	2.481101158110737E-4	7.190988102320948E-4	0.0	0.22869865319100727	2.5231537201126135E-5
56-57	2.5231537201126134E-4	7.317145788326579E-4	0.0	0.26956533294443125	2.5231537201126135E-5
58-59	2.5231537201126134E-4	7.317145788326579E-4	0.0	0.30054545537121397	2.5231537201126135E-5
60-61	2.56520628211449E-4	7.359198350328456E-4	0.0	0.3255120614317283	2.5231537201126135E-5
62-63	3.153942150140767E-4	7.527408598335963E-4	0.0	0.3447721348285879	2.5231537201126135E-5
64-65	3.195994712142644E-4	8.158197028364116E-4	0.0	0.34967966881420687	2.5231537201126135E-5
66-67	3.2380472741445204E-4	8.200249590365994E-4	0.0	0.3544610451138203	2.5231537201126135E-5
68-69	3.2800998361463975E-4	8.368459838373501E-4	0.0	0.3591120584712279	2.5231537201126135E-5
70-71	3.406257522152028E-4	8.494617524379132E-4	0.0	0.3635780405558272	2.5231537201126135E-5
72-73	3.9529408281764274E-4	8.494617524379132E-4	0.0	0.3673543606235958	3.364204960150151E-5
74-75	3.9529408281764274E-4	8.957195706399778E-4	0.0	0.37006254561651664	3.364204960150151E-5
76-77	3.9529408281764274E-4	9.377721326418547E-4	0.0	0.37269083074163395	3.364204960150151E-5
78-79	3.9529408281764274E-4	9.503879012424177E-4	0.0	0.37434770168450787	3.364204960150151E-5
80-81	3.9529408281764274E-4	9.503879012424177E-4	0.0	0.37484392191613003	3.364204960150151E-5
82-83	3.9529408281764274E-4	9.503879012424177E-4	0.0	0.37533173163535183	3.364204960150151E-5
84-85	3.9949933901783046E-4	9.58798413642793E-4	0.0	0.3755461997015614	3.364204960150151E-5
86-87	4.0370459521801817E-4	9.756194384435439E-4	0.0	0.375672357387567	3.364204960150151E-5
88	4.205256200187689E-4	0.0010513140500469221	0.0	0.37568917841236776	3.364204960150151E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19775	0.0	53.680065	1
GTATCAA	35915	0.0	37.53931	1
TCAACGC	44295	0.0	30.00253	4
ATCAACG	44315	0.0	29.94013	3
CAACGCA	45200	0.0	29.384703	5
TATCAAC	45615	0.0	29.22778	2
AACGCAG	46920	0.0	28.353188	6
GTGGTAT	8315	0.0	27.728317	1
TGGTATC	8385	0.0	26.065771	2
ACGCAGA	53640	0.0	24.801004	7
CGCAGAG	54375	0.0	24.450205	8
GTACATG	45950	0.0	21.54418	1
GCAGAGT	62075	0.0	21.217894	9
TACATGG	46220	0.0	21.186525	2
GAGTACT	35665	0.0	20.944834	12-13
ACATGGG	46995	0.0	20.29911	3
CTTATAC	11630	0.0	20.082615	1
CAGAGTA	60600	0.0	19.502743	10-11
GTACTTT	39255	0.0	19.029758	14-15
AGAGTAC	57165	0.0	18.4741	10-11
>>END_MODULE