FastQCFastQC Report
Fri 27 May 2016
SRR2139209_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139209_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11897954
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151550.053.390541
GTATCAA274000.037.4585841
TCAACGC334600.029.9423564
ATCAACG340600.029.4394113
CAACGCA344550.029.1053355
TATCAAC349650.028.9111792
AACGCAG353850.028.4254846
ACGCAGA406350.024.6682427
CGCAGAG439650.022.8001068
GTACATG405000.022.262181
TACATGG405300.021.9379142
CTTATAC116800.021.876361
ACATGGG408550.021.1000733
GCAGAGT487250.020.4408999
GAGTACT255050.020.05424112-13
GTGGTAT72950.019.5769161
TATACAC153900.018.978533
CATGGGG312550.018.9245534
CAGAGTA472750.018.91532510-11
TGGTATC72150.018.6015762
Produced by FastQC (version 0.11.5)