##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139209_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11897954
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52585310045744	33.0	33.0	33.0	33.0	33.0
2	32.5572322770789	33.0	33.0	33.0	33.0	33.0
3	32.57821151434944	33.0	33.0	33.0	33.0	33.0
4	36.4847806606077	37.0	37.0	37.0	37.0	37.0
5	36.5097250333965	37.0	37.0	37.0	37.0	37.0
6	36.498890649602444	37.0	37.0	37.0	37.0	37.0
7	36.4758439980521	37.0	37.0	37.0	37.0	37.0
8	36.468737566139524	37.0	37.0	37.0	37.0	37.0
9	36.50804961928748	37.0	37.0	37.0	37.0	37.0
10-11	36.428683914898315	37.0	37.0	37.0	37.0	37.0
12-13	36.42964546677521	37.0	37.0	37.0	37.0	37.0
14-15	38.431372906635886	40.0	37.0	40.0	37.0	40.0
16-17	38.38275534600319	40.0	37.0	40.0	37.0	40.0
18-19	38.380313497597996	40.0	37.0	40.0	37.0	40.0
20-21	38.37391410573555	40.0	37.0	40.0	37.0	40.0
22-23	38.373109044728224	40.0	37.0	40.0	37.0	40.0
24-25	38.357369951735095	40.0	37.0	40.0	37.0	40.0
26-27	38.2447386999132	40.0	37.0	40.0	37.0	40.0
28-29	38.17425942379852	40.0	37.0	40.0	37.0	40.0
30-31	38.038822936423614	40.0	37.0	40.0	33.0	40.0
32-33	38.04214276997345	40.0	37.0	40.0	33.0	40.0
34-35	38.00156301249873	40.0	37.0	40.0	33.0	40.0
36-37	37.91642742187189	40.0	37.0	40.0	33.0	40.0
38-39	37.85249137078138	40.0	37.0	40.0	33.0	40.0
40-41	37.85217096361987	40.0	37.0	40.0	33.0	40.0
42-43	37.78418184773567	40.0	37.0	40.0	33.0	40.0
44-45	37.715763345936395	40.0	37.0	40.0	33.0	40.0
46-47	37.66951188430747	40.0	37.0	40.0	33.0	40.0
48-49	37.63147755564678	40.0	37.0	40.0	33.0	40.0
50-51	37.52632498606819	40.0	37.0	40.0	33.0	40.0
52-53	37.41057939297221	40.0	37.0	40.0	33.0	40.0
54-55	37.24766011075394	40.0	37.0	40.0	33.0	40.0
56-57	37.13086473239774	37.0	37.0	40.0	33.0	40.0
58-59	36.94820775654472	37.0	37.0	40.0	33.0	40.0
60-61	36.81725713093168	37.0	37.0	40.0	33.0	40.0
62-63	36.64667656781454	37.0	37.0	40.0	33.0	40.0
64-65	36.46150916611087	37.0	37.0	40.0	33.0	40.0
66-67	36.22758757667923	37.0	37.0	40.0	33.0	40.0
68-69	36.01160825651463	37.0	37.0	38.5	33.0	40.0
70-71	35.76188411009366	37.0	33.0	37.0	33.0	40.0
72-73	35.556266608426256	37.0	33.0	37.0	33.0	40.0
74-75	35.34757470482068	37.0	33.0	37.0	33.0	40.0
76-77	33.63040783463994	35.0	33.0	37.0	27.0	37.0
78-79	34.873303983229235	37.0	33.0	37.0	33.0	37.0
80-81	34.966096067946104	37.0	33.0	37.0	33.0	37.0
82-83	34.8225970542061	37.0	33.0	37.0	33.0	37.0
84-85	34.70199700014742	37.0	33.0	37.0	33.0	37.0
86-87	34.59910661420651	37.0	33.0	37.0	33.0	37.0
88-89	34.459488402853225	37.0	33.0	37.0	30.0	37.0
90-91	34.46318025929189	37.0	33.0	37.0	33.0	37.0
92-93	34.393078736717165	37.0	33.0	37.0	33.0	37.0
94-95	34.372058220401584	37.0	33.0	37.0	33.0	37.0
96-97	34.38975768800776	37.0	33.0	37.0	33.0	37.0
98-99	34.38388210776807	37.0	33.0	37.0	33.0	37.0
100	34.40514878369111	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	15.0
20	105.0
21	543.0
22	2086.0
23	5992.0
24	13407.0
25	26251.0
26	45228.0
27	71390.0
28	103888.0
29	143690.0
30	191980.0
31	253675.0
32	333379.0
33	444429.0
34	604804.0
35	877191.0
36	1531075.0
37	3497945.0
38	3659466.0
39	91412.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.902448101581165	19.845378457506225	14.032597537358104	24.21957590355451
2	15.02342335497347	20.708190668748593	41.17574332528097	23.092642650996968
3	18.436279044279377	25.753192523689368	29.999048575914816	25.81147985611644
4	12.805277277084784	17.927225134674416	37.755701526497745	31.511796061743052
5	13.966006256201696	37.273248829168445	34.94805913689026	13.8126857777396
6	30.640307374166053	37.01040902704014	18.43619678092228	13.913086817871529
7	27.128353328647936	32.15497387197833	22.486891443688553	18.229781355685187
8	24.950096461963124	36.29291220994803	20.584993016446358	18.17199831164249
9	25.903151079588977	16.53766689634201	20.646524604146226	36.91265741992279
10-11	24.882636092521427	26.306880262737476	28.086072847131334	20.724410797609767
12-13	25.680405935390983	23.851591271691493	28.093060549703825	22.374942243213695
14-15	22.753330724700085	25.07491065375092	25.788701463357523	26.383057158191477
16-17	22.48300422072568	28.24898717880402	26.33026653153979	22.937742068930504
18-19	22.561736244735858	27.31618394221393	27.953381732691184	22.168698080359025
20-21	23.45596061586792	26.405156139479917	27.894387124914903	22.244496119737256
22-23	23.208202286117135	26.523131394849525	27.642536214758888	22.62613010427445
24-25	22.961650121785016	26.72468515399898	27.796229330808792	22.517435393407215
26-27	22.834077619504356	26.868223331605222	27.774306516939212	22.523392531951206
28-29	22.820777563456566	26.804953143816203	27.767761987979007	22.60650730474823
30-31	23.22248843428887	26.505485804082586	27.714161202282323	22.55786455934623
32-33	22.93372002337047	26.67323101604039	27.683154606456878	22.709894354132267
34-35	23.03298664857634	26.64616074543712	27.718018514006836	22.602834091979705
36-37	23.060939287696854	26.70504650871929	27.521575937805213	22.71243826577864
38-39	22.828995370481408	26.731021225254914	27.674169922430398	22.76581348183328
40-41	23.00223723857902	26.512765114098997	27.703495515610292	22.78150213171169
42-43	22.939025163234106	26.60959644987781	27.504115731723534	22.94726265516455
44-45	22.86647202072658	26.758412259095078	27.376012948605	22.999102771573344
46-47	23.11929046962514	26.64995584428039	27.069281095577647	23.161472590516823
48-49	23.114693867644633	26.862389973254274	26.92936550408216	23.09355065501893
50-51	23.00953317049219	26.9315503899006	26.94744270361833	23.111473735988884
52-53	23.19576284391953	26.889726738560384	26.945724690880056	22.968785726640032
54-55	23.200547506605425	26.804822318068904	26.830477149330694	23.16415302599498
56-57	23.266772152056138	27.031020671751083	26.60702475177752	23.095182424415263
58-59	23.02053830090022	27.099180559222596	26.88302005079617	22.99726108908101
60-61	23.20729223420354	27.20760745252529	26.605748950282575	22.97935136298859
62-63	23.11212103845599	27.297063409979653	26.666052217216613	22.924763334347745
64-65	23.17474376169207	27.143405694798716	26.594967117958777	23.086883425550432
66-67	23.255842220416177	27.13082815173065	26.455502979593305	23.15782664825987
68-69	23.193103122977398	27.036690072656267	26.546358713606967	23.223848090759372
70-71	23.18344706512206	26.878896598946707	26.58490336744064	23.352752968490602
72-73	23.19619944410438	26.945353966283097	26.43129667550571	23.42714991410681
74-75	23.131212120088023	26.986365330500554	26.468627237749427	23.413795311662
76-77	23.231764519908225	26.976868903101135	26.4058724256307	23.38549415135994
78-79	23.147695792357958	26.988681196849946	26.427208098783726	23.43641491200837
80-81	23.183041202789266	27.022158947005494	26.43492597963642	23.359873870568823
82-83	23.3116061387627	26.93241052975531	26.563910039624012	23.19207329185798
84-85	23.31802630296887	26.918001575361476	26.49595372007202	23.268018401597633
86-87	23.171725275417813	27.100121425635287	26.45965251082023	23.268500788126666
88-89	23.29045823001889	26.960068195375484	26.4150446324682	23.33442894213743
90-91	23.281536399910262	27.03385312234966	26.4231857967888	23.261424680951276
92-93	23.32076859369046	27.009175694040742	26.512784634640806	23.157271077627996
94-95	23.112729659113473	27.096737216925	26.600492355131305	23.19004076883022
96-97	23.16376467099307	27.149068382866385	26.656100186368864	23.031066759771676
98-99	24.42308351460977	29.04456455517031	26.757494368602824	19.774857561617093
100	23.166489949920415	30.059708328799434	20.615417123220077	26.15838459806008
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2771.0
1	2437.0
2	3048.0
3	4182.0
4	5444.5
5	8635.0
6	11467.5
7	13390.0
8	14622.5
9	14618.5
10	13699.0
11	12478.0
12	13328.5
13	17341.0
14	26994.5
15	40805.5
16	52559.0
17	59867.5
18	62038.0
19	58926.0
20	52312.0
21	44758.0
22	39490.5
23	39140.0
24	43591.5
25	53175.0
26	66995.0
27	83279.5
28	102569.5
29	124867.0
30	147538.5
31	169974.0
32	194580.5
33	219628.0
34	245060.0
35	269665.5
36	296032.0
37	322198.5
38	344371.0
39	365654.0
40	386093.5
41	404626.0
42	418685.5
43	430667.0
44	444723.5
45	466361.0
46	492131.0
47	517800.0
48	527294.5
49	524883.0
50	525268.0
51	520131.5
52	496999.5
53	464062.0
54	440503.0
55	417888.0
56	391413.0
57	359230.5
58	323430.0
59	284773.0
60	241304.5
61	203762.0
62	167875.0
63	135538.5
64	111084.5
65	87687.5
66	69427.5
67	51866.5
68	35442.0
69	25897.5
70	18554.0
71	12070.0
72	8317.0
73	6019.5
74	4470.0
75	3173.5
76	1972.5
77	1108.0
78	803.5
79	618.0
80	408.0
81	256.0
82	192.0
83	120.5
84	66.0
85	41.0
86	27.5
87	24.0
88	20.5
89	15.5
90	11.5
91	9.0
92	6.5
93	3.5
94	2.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0085767687452817E-4
7	0.0
8	0.0
9	0.0
10-11	0.003786365285997912
12-13	1.2607209609316022E-5
14-15	0.002357548196942096
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	233.0
20-21	725.0
22-23	1738.0
24-25	4336.0
26-27	4939.0
28-29	7907.0
30-31	19245.0
32-33	23945.0
34-35	34266.0
36-37	49709.0
38-39	58383.0
40-41	63267.0
42-43	76581.0
44-45	91637.0
46-47	89380.0
48-49	80575.0
50-51	69915.0
52-53	66598.0
54-55	75566.0
56-57	83629.0
58-59	85376.0
60-61	87830.0
62-63	88182.0
64-65	94007.0
66-67	100864.0
68-69	94154.0
70-71	91758.0
72-73	87229.0
74-75	91084.0
76-77	88670.0
78-79	95810.0
80-81	99373.0
82-83	96550.0
84-85	97610.0
86-87	104118.0
88-89	111194.0
90-91	118158.0
92-93	124717.0
94-95	152217.0
96-97	438322.0
98-99	744737.0
100-101	7903420.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.59173401022176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.44442322292466	73.04778277515274
2	7.93878607898343	13.113562163989018
3	1.972211174655773	4.886650222474698
4	0.7306253076742145	2.4137444429026065
5	0.3324276208536461	1.3727886819597592
6	0.1841836185686113	0.9127226660315331
7	0.11078376151061138	0.640487607433538
8	0.06456874955924874	0.42662759911760767
9	0.04222274646119363	0.31385248604135424
>10	0.1722138875382305	2.3073434059680817
>50	0.005761895828666164	0.31590134525926133
>100	0.0017722344023017513	0.2378625455188326
>500	1.9701039460178008E-5	0.010674058150978577
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.404806406210682E-6	8.404806406210682E-6
2	0.0	5.042883843726409E-5	0.0	1.6809612812421364E-5	8.404806406210682E-6
3	0.0	5.883364484347477E-5	0.0	1.6809612812421364E-5	8.404806406210682E-6
4	0.0	1.428817089055816E-4	0.0	3.361922562484273E-5	8.404806406210682E-6
5	0.0	1.6809612812421362E-4	0.0	4.2024032031053404E-5	8.404806406210682E-6
6	0.0	1.6809612812421362E-4	0.0	4.2024032031053404E-5	8.404806406210682E-6
7	0.0	1.8490574093663498E-4	0.0	5.042883843726409E-5	8.404806406210682E-6
8	0.0	2.0171535374905635E-4	0.0	5.042883843726409E-5	8.404806406210682E-6
9	0.0	4.034307074981127E-4	0.0	9.245287046831749E-5	8.404806406210682E-6
10-11	0.0	4.034307074981127E-4	0.0	1.0506008007763352E-4	8.404806406210682E-6
12-13	0.0	4.118355139043234E-4	0.0	1.0926248328073886E-4	8.404806406210682E-6
14-15	0.0	6.303604804658011E-4	0.0	1.2607209609316023E-4	1.2607209609316022E-5
16-17	0.0	6.639797060906438E-4	0.0	1.5128651531179228E-4	1.6809612812421364E-5
18-19	0.0	7.270157541372239E-4	0.0	2.1852496656147772E-4	1.6809612812421364E-5
20-21	0.0	8.026590117931201E-4	0.0	2.5214419218632045E-4	1.6809612812421364E-5
22-23	0.0	8.825046726521216E-4	0.0	2.773586114049525E-4	1.6809612812421364E-5
24-25	0.0	9.455407206987017E-4	0.0	3.025730306235845E-4	1.6809612812421364E-5
26-27	0.0	9.833623495266498E-4	0.0	3.8241869148258596E-4	1.6809612812421364E-5
28-29	0.0	9.917671559328603E-4	0.0	8.278734310117521E-4	1.6809612812421364E-5
30-31	0.0	0.0011388512680415475	0.0	0.0022650953264737785	1.6809612812421364E-5
32-33	0.0	0.0011640656872601795	0.0	0.006068270225284111	1.6809612812421364E-5
34-35	0.0	0.0013279594121812877	0.0	0.011127963681822943	1.6809612812421364E-5
36-37	0.0	0.0015002579435086066	0.0	0.01958319892647089	1.6809612812421364E-5
38-39	0.0	0.001567496394758292	0.0	0.04041030920106095	1.6809612812421364E-5
40-41	0.0	0.0017818189581166645	0.0	0.06254436687181678	1.6809612812421364E-5
42-43	0.0	0.0018196405869446125	0.0	0.07661821519901657	1.6809612812421364E-5
44-45	0.0	0.002012951134287458	0.0	0.09398254523424784	1.6809612812421364E-5
46-47	0.0	0.0021684400528023556	0.0	0.11121239836697974	1.6809612812421364E-5
48-49	0.0	0.0021684400528023556	0.0	0.12767741411674646	1.6809612812421364E-5
50-51	0.0	0.002370155406551412	0.0	0.14344062853159462	1.6809612812421364E-5
52-53	0.0	0.0023869650193638335	0.0	0.1837290680397655	2.1012016015526702E-5
54-55	0.0	0.002428989051394887	0.0	0.23233826589008497	2.5214419218632044E-5
56-57	0.0	0.0024878226962383614	0.0	0.2817417179457914	2.9416822421737385E-5
58-59	0.0	0.002504632309050783	0.0	0.3142683187378267	3.361922562484273E-5
60-61	4.202403203105341E-6	0.002542453937878731	0.0	0.33535597801100925	4.2024032031053404E-5
62-63	8.404806406210682E-6	0.002597085179519101	0.0	0.34933317106453765	4.2024032031053404E-5
64-65	8.404806406210682E-6	0.0029080630165488957	0.0	0.3544012693274827	4.2024032031053404E-5
66-67	8.404806406210682E-6	0.0029668966613923707	0.0	0.35910796091496067	4.2024032031053404E-5
68-69	8.404806406210682E-6	0.003063551935063793	0.0	0.36381465250243866	4.6226435234158746E-5
70-71	8.404806406210682E-6	0.0031055759670948467	0.0	0.3677733163197639	5.042883843726409E-5
72-73	8.404806406210682E-6	0.0031265879831103736	0.0	0.37105119081818605	7.564325765589614E-5
74-75	8.404806406210682E-6	0.0032022312407662696	0.0	0.3737785504970014	7.564325765589614E-5
76-77	8.404806406210682E-6	0.0033241009336563245	0.0	0.37642606451495775	7.984566085900147E-5
78-79	8.404806406210682E-6	0.003403946594515326	0.0	0.37834656277877693	8.404806406210681E-5
80-81	8.404806406210682E-6	0.003416553804124642	0.0	0.3790357569040862	8.404806406210681E-5
82-83	1.2607209609316022E-5	0.003429161013733958	0.0	0.3795316404820526	8.404806406210681E-5
84-85	1.6809612812421364E-5	0.0034459706265463795	0.0	0.37978378467423896	8.404806406210681E-5
86-87	1.6809612812421364E-5	0.0035216138842022755	0.0	0.3799350711895507	8.404806406210681E-5
88	1.6809612812421364E-5	0.0036560907867016464	0.0	0.3799812976247849	8.404806406210681E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15155	0.0	53.39054	1
GTATCAA	27400	0.0	37.458584	1
TCAACGC	33460	0.0	29.942356	4
ATCAACG	34060	0.0	29.439411	3
CAACGCA	34455	0.0	29.105335	5
TATCAAC	34965	0.0	28.911179	2
AACGCAG	35385	0.0	28.425484	6
ACGCAGA	40635	0.0	24.668242	7
CGCAGAG	43965	0.0	22.800106	8
GTACATG	40500	0.0	22.26218	1
TACATGG	40530	0.0	21.937914	2
CTTATAC	11680	0.0	21.87636	1
ACATGGG	40855	0.0	21.100073	3
GCAGAGT	48725	0.0	20.440899	9
GAGTACT	25505	0.0	20.054241	12-13
GTGGTAT	7295	0.0	19.576916	1
TATACAC	15390	0.0	18.97853	3
CATGGGG	31255	0.0	18.924553	4
CAGAGTA	47275	0.0	18.915325	10-11
TGGTATC	7215	0.0	18.601576	2
>>END_MODULE