FastQCFastQC Report
Fri 27 May 2016
SRR2139208_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139208_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8002660
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101050.052.2447431
GTATCAA187100.039.0493851
TCAACGC223100.031.924174
ATCAACG225050.031.6670073
CAACGCA228750.031.1553825
TATCAAC234900.030.5068452
AACGCAG236200.030.1541736
ACGCAGA269900.026.4053127
CGCAGAG275650.025.8548268
GTACATG258750.022.1288591
GCAGAGT322100.022.0206039
TACATGG259800.021.6181812
ACATGGG266400.020.6882213
GAGTACT179250.020.54156712-13
AGAGTAC291100.019.47746710-11
CAGAGTA314300.019.46759610-11
GTGGTAT47450.019.4660531
CATGGGG194300.018.834984
GTACTTT203550.017.99278514-15
TGGTATC47550.017.8600582
Produced by FastQC (version 0.11.5)