##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139208_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8002660
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5684304968598	33.0	33.0	33.0	33.0	33.0
2	32.57868308787328	33.0	33.0	33.0	33.0	33.0
3	32.57307432778601	33.0	33.0	33.0	33.0	33.0
4	36.47118533087748	37.0	37.0	37.0	37.0	37.0
5	36.50104577727905	37.0	37.0	37.0	37.0	37.0
6	36.494738249532034	37.0	37.0	37.0	37.0	37.0
7	36.471031507023916	37.0	37.0	37.0	37.0	37.0
8	36.45397155445814	37.0	37.0	37.0	37.0	37.0
9	36.49243126660385	37.0	37.0	37.0	37.0	37.0
10-11	36.44628392309557	37.0	37.0	37.0	37.0	37.0
12-13	36.37805673363607	37.0	37.0	37.0	37.0	37.0
14-15	38.415974813374554	40.0	37.0	40.0	37.0	40.0
16-17	38.37309438361744	40.0	37.0	40.0	37.0	40.0
18-19	38.37654211224768	40.0	37.0	40.0	37.0	40.0
20-21	38.37108341188805	40.0	37.0	40.0	37.0	40.0
22-23	38.367159780034044	40.0	37.0	40.0	37.0	40.0
24-25	38.36691552534135	40.0	37.0	40.0	37.0	40.0
26-27	38.22005498988373	40.0	37.0	40.0	37.0	40.0
28-29	38.1389151229814	40.0	37.0	40.0	35.0	40.0
30-31	38.10177854240992	40.0	37.0	40.0	33.0	40.0
32-33	38.0232967725008	40.0	37.0	40.0	33.0	40.0
34-35	37.98368923947088	40.0	37.0	40.0	33.0	40.0
36-37	37.9474641553067	40.0	37.0	40.0	33.0	40.0
38-39	37.89532928782835	40.0	37.0	40.0	33.0	40.0
40-41	37.860224923074895	40.0	37.0	40.0	33.0	40.0
42-43	37.76295023988489	40.0	37.0	40.0	33.0	40.0
44-45	37.7090852595675	40.0	37.0	40.0	33.0	40.0
46-47	37.668134707047315	40.0	37.0	40.0	33.0	40.0
48-49	37.63441893439894	40.0	37.0	40.0	33.0	40.0
50-51	37.55788230371817	40.0	37.0	40.0	33.0	40.0
52-53	37.45389854758016	40.0	37.0	40.0	33.0	40.0
54-55	37.23624543235027	40.0	37.0	40.0	33.0	40.0
56-57	37.04685636554652	37.0	37.0	40.0	33.0	40.0
58-59	36.97827040751764	37.0	37.0	40.0	33.0	40.0
60-61	36.83564499474203	37.0	37.0	40.0	33.0	40.0
62-63	36.644127421855956	37.0	37.0	40.0	33.0	40.0
64-65	36.48756001551047	37.0	37.0	40.0	33.0	40.0
66-67	36.266478403141264	37.0	37.0	40.0	33.0	40.0
68-69	36.042183736659	37.0	37.0	40.0	33.0	40.0
70-71	35.77552683930512	37.0	35.0	37.0	33.0	40.0
72-73	35.56680214058664	37.0	33.0	37.0	33.0	40.0
74-75	35.389397493487415	37.0	33.0	37.0	33.0	40.0
76-77	33.605010755746314	35.0	33.0	37.0	27.0	37.0
78-79	34.85900494420531	37.0	33.0	37.0	33.0	37.0
80-81	34.92686866252777	37.0	33.0	37.0	33.0	37.0
82-83	34.82456061563918	37.0	33.0	37.0	33.0	37.0
84-85	34.681778567221826	37.0	33.0	37.0	33.0	37.0
86-87	34.56322035131819	37.0	33.0	37.0	33.0	37.0
88-89	34.49023067611893	37.0	33.0	37.0	33.0	37.0
90-91	34.406310110490836	37.0	33.0	37.0	30.0	37.0
92-93	34.340768301170826	37.0	33.0	37.0	30.0	37.0
94-95	34.330478035636624	37.0	33.0	37.0	27.0	37.0
96-97	34.34764861388037	37.0	33.0	37.0	33.0	37.0
98-99	34.36409088640259	37.0	33.0	37.0	33.0	37.0
100	34.373920055024236	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	8.0
20	65.0
21	374.0
22	1371.0
23	4097.0
24	9215.0
25	18104.0
26	31474.0
27	49860.0
28	72679.0
29	100444.0
30	134629.0
31	176502.0
32	230199.0
33	304442.0
34	412933.0
35	591398.0
36	1013458.0
37	2322141.0
38	2480615.0
39	48650.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.03958433820755	19.752819687453922	14.167064451070019	24.040531523268513
2	14.804589973833702	20.658031204624464	41.65172580117111	22.88565302037073
3	18.513731684215	25.827974698412774	29.935134067922416	25.723159549449807
4	12.60678324457118	17.821824243439057	38.33285432593663	31.238538186053137
5	13.689748158737219	37.63759799866545	35.10420285255152	13.568450990045811
6	30.52611638206751	37.35714591862529	18.56304260871293	13.553695090594264
7	27.008669617352233	32.40728707704688	22.55408826565167	18.029955039949215
8	24.82357116258844	36.580062129342	20.668452739464126	17.927913968605438
9	25.846506036742785	16.796452679484073	20.61334606243424	36.7436952213389
10-11	24.648831961988087	26.535800773841466	28.366125095102575	20.449242169067873
12-13	25.605272210829455	23.973566719678345	28.447128503484915	21.97403256600729
14-15	22.575779492400887	25.253988571100084	26.14630004127051	26.023931895228525
16-17	22.192352292862623	28.405880044885073	26.748431146643743	22.65333651560856
18-19	22.342090005073313	27.49527032261773	28.426023347237045	21.736616325071914
20-21	23.082873656544667	26.69890261766627	28.3425237450644	21.875699980724658
22-23	22.816454788584622	26.6629900350411	28.206238039153124	22.314317137221153
24-25	22.501556788987408	26.821143131841968	28.33463451144324	22.342665567727384
26-27	22.512073930325478	26.945316570344875	28.18809337364499	22.354516125684654
28-29	22.46372103009204	26.97571612499907	28.19144521817539	22.369117626733505
30-31	22.763052849222515	26.819165733844603	28.15243531799193	22.265346098940945
32-33	22.58949368774861	26.90366646820327	28.144167137343644	22.362672706704476
34-35	22.60003998976282	26.97892529457933	28.157462264211336	22.263572451446517
36-37	22.696029120924635	26.899380457649574	28.02522927859819	22.379361142827598
38-39	22.5221871651357	27.070663275478207	28.054492374116265	22.35265718526983
40-41	22.699595725671028	26.92289959866419	27.98394555431231	22.393559121352467
42-43	22.669629233674623	27.001750609704867	27.7596209274536	22.568999229166913
44-45	22.649610802595816	27.12451439058953	27.648262038566333	22.57761276824832
46-47	22.887172720129342	27.06196672442126	27.343168798283337	22.70769175716606
48-49	22.758688168503088	27.16788649384332	27.325454069566472	22.747971268087117
50-51	22.68125238432364	27.357127941821556	27.191619500005505	22.7700001738493
52-53	22.847232039825847	27.291436318699684	27.143651626930204	22.717680014544264
54-55	22.858531299890412	27.149645732062588	27.15020445209153	22.841618515955467
56-57	22.822277917603582	27.333954014527638	27.07904963092816	22.76471843694062
58-59	22.758881998124235	27.334013551751106	27.124405292905585	22.78269915721907
60-61	22.945189256715885	27.252552096419958	27.159372538491112	22.64288610837304
62-63	22.73444461231136	27.400271944402476	27.100159473569352	22.765123969716807
64-65	22.696365108763462	27.407903258416884	27.051796037829078	22.843935594990576
66-67	22.801434299914625	27.34637572672554	27.075768892822776	22.776421080537055
68-69	22.717008113070314	27.44487358847847	26.975136330451143	22.862981968000074
70-71	22.781945613984448	27.221700542545328	26.956235487019477	23.040118356450748
72-73	22.760120835494956	27.277598508649074	26.86770940254674	23.094571253309226
74-75	22.599754265775253	27.42323293800296	26.96036268683718	23.016650109384603
76-77	22.83489206133445	27.23610237775228	26.917400692529466	23.01160486838381
78-79	22.84551493349736	27.205935466772452	26.714290401160824	23.234259198569365
80-81	22.891636827998354	27.354295332765272	26.81315127343551	22.94091656580087
82-83	22.89250176266445	27.22755927400455	26.82024747323672	23.059691490094288
84-85	22.918957820513597	27.222220477160953	26.743347256243815	23.115474446081635
86-87	22.75434500709696	27.35557380983691	26.879919884278603	23.010161298787533
88-89	23.04300822092834	27.30833733014006	26.840697916521666	22.80795653240994
90-91	23.015412845224155	27.353174169282358	26.81936056901187	22.812052416481617
92-93	22.84133163321015	27.22322248877384	26.87893383819256	23.056512039823453
94-95	22.846641974289426	27.368402372844606	26.971274568767473	22.81368108409849
96-97	23.064318295669946	27.328368747907472	27.000746701674274	22.606566254748305
98-99	23.990174006255163	29.371070448315244	27.223167486855637	19.415588058573956
100	22.93948680558611	30.37824674887379	21.01791250894548	25.66435393659462
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1088.0
1	1043.0
2	1647.5
3	2463.0
4	3332.5
5	5561.5
6	7561.5
7	8870.5
8	9566.5
9	9270.0
10	8474.0
11	7655.5
12	8329.5
13	11218.5
14	18219.0
15	28117.5
16	36454.5
17	41582.5
18	42653.0
19	39597.5
20	34474.5
21	29213.5
22	25681.0
23	25271.5
24	28065.0
25	34429.0
26	44269.0
27	55930.0
28	68904.0
29	84422.0
30	100757.0
31	117345.5
32	136474.5
33	155721.0
34	173948.5
35	191932.5
36	211965.0
37	230747.5
38	244991.5
39	257061.0
40	270090.5
41	282547.0
42	291305.5
43	299788.5
44	308545.5
45	317770.5
46	323666.0
47	327649.0
48	331498.5
49	331105.0
50	328966.0
51	323127.0
52	311945.0
53	301965.0
54	289479.5
55	269269.5
56	246604.5
57	224086.5
58	201327.0
59	178019.0
60	150526.5
61	122378.5
62	97090.0
63	74404.5
64	55607.5
65	42000.0
66	31684.0
67	22418.5
68	16051.0
69	11275.5
70	7889.0
71	5712.5
72	4036.0
73	2870.0
74	2015.0
75	1326.0
76	843.0
77	543.5
78	377.0
79	243.0
80	156.5
81	109.0
82	84.5
83	67.5
84	54.0
85	46.5
86	37.5
87	28.0
88	20.0
89	12.5
90	7.0
91	5.0
92	3.5
93	1.5
94	0.5
95	1.0
96	1.0
97	1.0
98	1.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.2495845131493777E-5
7	0.0
8	0.0
9	0.0
10-11	0.0014557659578190252
12-13	2.624127477613694E-4
14-15	0.007216350563437657
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	133.0
20-21	433.0
22-23	950.0
24-25	2427.0
26-27	2868.0
28-29	4161.0
30-31	9305.0
32-33	11754.0
34-35	16173.0
36-37	22828.0
38-39	28574.0
40-41	32761.0
42-43	40210.0
44-45	49135.0
46-47	48259.0
48-49	43673.0
50-51	37577.0
52-53	35464.0
54-55	38430.0
56-57	42769.0
58-59	28436.0
60-61	48464.0
62-63	46903.0
64-65	49123.0
66-67	53525.0
68-69	53884.0
70-71	55253.0
72-73	52608.0
74-75	53767.0
76-77	52729.0
78-79	58387.0
80-81	61395.0
82-83	60619.0
84-85	60726.0
86-87	64837.0
88-89	71002.0
90-91	76248.0
92-93	83318.0
94-95	101899.0
96-97	309500.0
98-99	523698.0
100-101	5568455.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.93018669025896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.1863510930924	75.74613448559319
2	7.7266584511223755	13.12453089491381
3	1.7898812153119652	4.560448373094983
4	0.6201603332912592	2.1068133153727944
5	0.2722022083600301	1.1559092186759063
6	0.1330802512556176	0.6781518350355702
7	0.0813525622092664	0.4836501807314731
8	0.053565225534520554	0.3639443683802125
9	0.03247817868547857	0.24825400012055562
>10	0.10177380823483528	1.3257395433106178
>50	0.001953245442620914	0.11065502107822864
>100	5.199808884792704E-4	0.08137150806156182
>500	2.3446571138620554E-5	0.01439725563107775
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2495845131493779E-5
2	0.0	2.4991690262987558E-5	0.0	0.0	2.4991690262987558E-5
3	0.0	2.4991690262987558E-5	0.0	0.0	2.4991690262987558E-5
4	0.0	4.9983380525975116E-5	0.0	0.0	2.4991690262987558E-5
5	0.0	4.9983380525975116E-5	0.0	0.0	2.4991690262987558E-5
6	7.497507078896267E-5	4.9983380525975116E-5	0.0	0.0	4.9983380525975116E-5
7	7.497507078896267E-5	4.9983380525975116E-5	0.0	1.2495845131493779E-5	4.9983380525975116E-5
8	7.497507078896267E-5	4.9983380525975116E-5	0.0	1.2495845131493779E-5	4.9983380525975116E-5
9	7.497507078896267E-5	6.247922565746889E-5	0.0	2.4991690262987558E-5	7.497507078896267E-5
10-11	7.497507078896267E-5	6.247922565746889E-5	0.0	3.7487535394481335E-5	7.497507078896267E-5
12-13	7.497507078896267E-5	6.247922565746889E-5	0.0	3.7487535394481335E-5	7.497507078896267E-5
14-15	7.497507078896267E-5	9.996676105195023E-5	0.0	3.7487535394481335E-5	7.497507078896267E-5
16-17	9.996676105195023E-5	9.996676105195023E-5	0.0	3.7487535394481335E-5	7.497507078896267E-5
18-19	9.996676105195023E-5	1.0621468361769711E-4	0.0	3.7487535394481335E-5	8.747091592045645E-5
20-21	1.0621468361769711E-4	1.2495845131493778E-4	0.0	3.7487535394481335E-5	8.747091592045645E-5
22-23	1.12462606183444E-4	1.4370221901217846E-4	0.0	6.247922565746889E-5	9.996676105195022E-5
24-25	1.12462606183444E-4	1.8743767697240667E-4	0.0	1.2495845131493778E-4	1.12462606183444E-4
26-27	1.12462606183444E-4	1.9993352210390046E-4	0.0	1.68693909275166E-4	1.2495845131493778E-4
28-29	1.12462606183444E-4	1.9993352210390046E-4	0.0	3.1864405085309136E-4	1.2495845131493778E-4
30-31	1.12462606183444E-4	2.1242936723539423E-4	0.0	0.0010496509910454773	1.312063738806847E-4
32-33	1.12462606183444E-4	2.1242936723539423E-4	0.0	0.0031801925859651662	1.4995014157792534E-4
34-35	1.187105287491909E-4	2.5616482519562247E-4	0.0	0.005866799289236328	1.4995014157792534E-4
36-37	1.2495845131493778E-4	2.999002831558507E-4	0.0	0.010046659485720998	1.4995014157792534E-4
38-39	1.2495845131493778E-4	3.248919734188382E-4	0.0	0.023498436769774048	1.624459867094191E-4
40-41	1.2495845131493778E-4	3.3738781855033204E-4	0.0	0.03889331797177438	1.624459867094191E-4
42-43	1.2495845131493778E-4	3.436357411160789E-4	0.0	0.04862133340664229	1.624459867094191E-4
44-45	1.2495845131493778E-4	4.061149667735478E-4	0.0	0.06055486550721885	1.624459867094191E-4
46-47	1.2495845131493778E-4	4.623462698652698E-4	0.0	0.07298823141305516	1.624459867094191E-4
48-49	1.2495845131493778E-4	4.623462698652698E-4	0.0	0.08529663886757653	1.8743767697240667E-4
50-51	1.2495845131493778E-4	5.748088760487138E-4	0.0	0.09791744245038525	1.8743767697240667E-4
52-53	1.2495845131493778E-4	5.873047211802075E-4	0.0	0.12805742090754824	2.0618144466964735E-4
54-55	1.2495845131493778E-4	5.873047211802075E-4	0.0	0.16467024714282502	2.24925212366888E-4
56-57	1.2495845131493778E-4	5.873047211802075E-4	0.0	0.20086446256619672	2.24925212366888E-4
58-59	1.2495845131493778E-4	5.873047211802075E-4	0.0	0.3352697728005438	2.4366898006412865E-4
60-61	1.312063738806847E-4	5.935526437459544E-4	0.0	0.45281818795250584	2.7490859289286315E-4
62-63	1.3745429644643157E-4	6.122964114431952E-4	0.0	0.4576853196312226	2.999002831558507E-4
64-65	1.3745429644643157E-4	6.872714822321578E-4	0.0	0.46155278369941993	2.999002831558507E-4
66-67	1.3745429644643157E-4	6.997673273636516E-4	0.0	0.4653827602322228	3.1864405085309136E-4
68-69	1.3745429644643157E-4	7.060152499293985E-4	0.0	0.46940017444199805	3.3738781855033204E-4
70-71	1.3745429644643157E-4	7.122631724951453E-4	0.0	0.4733738531938131	3.3738781855033204E-4
72-73	1.3745429644643157E-4	7.122631724951453E-4	0.0	0.47632912056741133	3.498836636818258E-4
74-75	1.3745429644643157E-4	7.497507078896267E-4	0.0	0.4788845208968018	3.498836636818258E-4
76-77	1.3745429644643157E-4	7.87238243284108E-4	0.0	0.4813024669297459	3.561315862475727E-4
78-79	1.3745429644643157E-4	8.247257786785894E-4	0.0	0.4829144309517086	3.6237950881331957E-4
80-81	1.3745429644643157E-4	8.4346954637583E-4	0.0	0.48348923982775727	3.8737119907630716E-4
82-83	1.3745429644643157E-4	8.497174689415769E-4	0.0	0.48383287556887333	4.123628893392947E-4
84-85	1.3745429644643157E-4	8.622133140730707E-4	0.0	0.48410778416176625	4.123628893392947E-4
86-87	1.3745429644643157E-4	8.684612366388175E-4	0.0	0.48422024676794967	4.123628893392947E-4
88	1.3745429644643157E-4	8.99700849467552E-4	0.0	0.48422649469051543	4.123628893392947E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10105	0.0	52.244743	1
GTATCAA	18710	0.0	39.049385	1
TCAACGC	22310	0.0	31.92417	4
ATCAACG	22505	0.0	31.667007	3
CAACGCA	22875	0.0	31.155382	5
TATCAAC	23490	0.0	30.506845	2
AACGCAG	23620	0.0	30.154173	6
ACGCAGA	26990	0.0	26.405312	7
CGCAGAG	27565	0.0	25.854826	8
GTACATG	25875	0.0	22.128859	1
GCAGAGT	32210	0.0	22.020603	9
TACATGG	25980	0.0	21.618181	2
ACATGGG	26640	0.0	20.688221	3
GAGTACT	17925	0.0	20.541567	12-13
AGAGTAC	29110	0.0	19.477467	10-11
CAGAGTA	31430	0.0	19.467596	10-11
GTGGTAT	4745	0.0	19.466053	1
CATGGGG	19430	0.0	18.83498	4
GTACTTT	20355	0.0	17.992785	14-15
TGGTATC	4755	0.0	17.860058	2
>>END_MODULE